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“Spike sorting”
Kenneth D. HarrisUniversity College London
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Brain contains billions of neurons
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United states BRAIN initiative: $1billion
EU Human Brain project: €1billion
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Silicon Microelectrodes
www.neuronexustech.com
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Extracellular voltage field
C. Gold et al, J. Neurophysiol 2006
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“Tetrode effect”Different cells have different waveform/amplitude profiles across channels
Allows about 15 neurons to be distinguished by a single tetrode
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Traditional pipeline
Einevoll et al, Curr Opp Neuro 2012
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Traditional clustering approach
https://github.com/klusta-team/klustaviewa
1. Mixture of Gaussians fit (12 dimensions)2. Manual verification and adjustment
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Error rates
Harris et al, J. Neurophysiol, 2000
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High density probes
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One approach• Local spike detection based on spatiotemporal continuity
• “Masked EM algorithm” for cluster analysis based on local features
http://arxiv.org/abs/1309.2848https://github.com/klusta-team/klustakwik
“Mask vectors”show which
channels have data
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Thanks!UCL:Cyrille Rossant (KlustaViewa)Shabnam Kadir (KlustaKwik, SpikeDetekt)
Harvard: Dan Goodman (KlustaKwik, SpikeDetekt)
Berkeley:John Schulman (SpikeDetekt)
NYU:Gyorgy Buzsaki, Mariano Beluscio (Data)