TG|WT_06 TG|WT_08 TG|WT_10 TG|WT_12 TG|WT_14 TG|WT_18 TG|WT_22 TG|WT_26

Adipic acid, 2-amino- 0.949 0.749 0.851 1.416 1.145 1.070 4.129 1.730

Cysteine 1.271 1.658 0.668 2.429 1.116 1.843 0.568 0.606

Methionine 1.749 0.668 0.545 2.197 1.003 0.921 0.624 1.149

Tryptophan 0.784 0.253 0.235 0.748 0.454 1.099 2.081 1.622

Phosphoenolpyruvic acid 0.259 NaN 1.353 1.232 1.278 1.486 1.455 2.064

Asparagine 0.845 1.178 1.105 1.535 1.004 0.687 0.889 0.719

Aspartic acid 1.181 0.597 0.670 1.455 0.955 0.643 0.439 0.762

Threonine 0.901 0.650 0.585 1.430 1.086 0.731 0.612 1.106

Homoserine 0.969 0.761 0.557 1.647 0.912 0.663 0.605 1.237

Ascorbic acid 0.572 0.594 1.152 0.624 0.545 0.671 0.336 0.658

Serine 0.959 0.853 0.598 1.123 0.918 0.841 0.691 0.747

Valine 0.929 0.800 0.494 1.191 0.977 1.116 0.616 0.657

Phenylalanine 0.818 0.641 0.386 1.266 0.712 0.800 0.596 0.485

Isoleucine 0.875 0.462 0.366 0.997 0.825 0.989 0.816 0.771

Maltose 1.051 0.651 0.566 0.742 0.924 0.790 0.656 0.902

Erythronic acid-1,4-lactone 0.576 0.828 0.746 0.812 0.955 1.051 0.924 0.881

Erythronic acid 0.440 0.649 0.658 0.873 0.948 1.175 0.878 0.844

Propane, 1,2-diamino- or 1,3-diamino- 0.686 0.649 0.748 0.877 1.027 0.998 0.712 1.045

Threonic acid 0.707 0.787 0.595 0.899 1.023 1.236 0.834 0.968

Glycine 0.781 0.756 0.932 1.290 0.880 0.940 0.905 0.749

Adenosine, 5-methylthio- 0.987 0.881 0.835 1.035 0.925 1.001 0.736 0.835

Inositol-phosphate 0.889 0.953 0.834 1.015 0.915 1.026 0.758 0.847

Glyceric acid 0.838 0.818 0.858 0.950 0.835 0.931 0.738 0.856

Fructose-1-phosphate NaN 0.620 0.986 1.009 0.836 1.180 0.920 0.985

Trehalose, alpha,alpha'- 0.879 0.567 0.880 0.940 0.891 1.000 1.063 0.977

Proline 0.942 0.505 0.489 1.065 1.954 1.146 0.747 0.634

Alanine 0.968 1.257 1.293 1.549 1.670 1.141 1.153 1.017

Uracil 1.651 1.227 1.304 1.366 1.454 1.109 1.020 0.985

Malic acid, 2-isopropyl- 1.071 0.965 1.358 1.079 1.201 1.163 0.959 0.851

Pyroglutamic acid (Glutamine, Glutamic acid)* 1.193 1.045 0.980 1.157 1.214 1.241 0.923 1.148

Glyceric acid-3-phosphate 1.290 0.883 0.987 1.186 0.911 1.236 0.727 1.155

Glycerophosphoglycerol 1.364 0.745 0.867 1.331 1.201 1.168 0.908 0.868

Butyric acid, 4-amino- 0.859 0.741 1.001 0.989 1.362 0.997 1.254 1.132

Galactonic acid 0.895 0.827 0.989 0.967 1.222 1.049 1.122 1.024

Lysine 0.966 0.976 0.688 1.177 0.956 1.077 1.739 1.174

beta-D-Fructofuranosyl-(2,1)-beta-D-Fructofuranose 0.848 0.972 0.945 1.028 0.897 1.312 1.532 1.298

Leucine 0.997 0.851 0.610 0.855 0.975 0.958 1.441 1.622

Tyrosine 1.204 0.979 0.624 0.877 0.914 0.944 1.297 1.323

Phosphoric acid monomethyl ester 0.907 1.034 0.782 0.766 0.965 1.164 0.931 1.579

Ornithine (Arginine, Citrulline)* 1.213 1.241 0.856 1.119 0.987 0.921 1.134 1.185

Arginine (Citrulline)* 1.094 1.385 0.939 1.261 0.930 0.771 1.205 1.230

Glutamine 1.160 1.228 0.936 1.178 1.183 0.999 1.072 1.385

Indole-3-acetic acid NaN NaN NaN 1.087 1.217 0.973 1.181 1.477

Glutaric acid, 2-oxo- 1.273 0.930 0.882 0.952 0.930 1.072 1.150 1.447

Glycerol-3-phosphate 1.223 0.918 1.015 0.998 1.038 0.926 1.128 1.412

Saccharic acid 1.456 1.246 0.968 0.910 0.959 1.058 0.881 1.449

Galactinol 1.316 1.391 0.957 0.869 1.126 1.282 1.315 1.465

Trehalose-6-phosphate 1.107 1.187 0.757 0.692 0.584 0.742 0.692 1.188

Malonic acid 1.229 0.746 0.841 0.957 0.765 0.920 0.862 1.067

Ethanolaminephosphate 1.169 0.765 0.766 0.984 1.155 0.843 1.000 1.084

Spermidine 0.835 0.700 0.558 0.893 0.911 0.986 1.061 1.048

Succinic acid 0.959 0.810 0.840 0.933 0.985 0.842 1.191 1.021

Sorbitol 0.888 0.736 0.730 0.911 0.905 1.002 1.217 0.874

Putrescine (Agmatine)* 0.899 0.867 0.875 1.033 0.951 1.301 0.939 0.937

Erythritol 0.879 0.914 0.839 0.959 0.913 1.114 0.935 0.966

Alanine, beta- 1.006 0.948 1.059 1.185 0.954 0.995 0.902 1.035

Glucopyranoside, 1-O-methyl- 1.102 1.098 0.956 1.061 0.867 1.159 0.917 1.132

Fumaric acid 1.057 1.080 0.957 0.947 1.102 0.923 1.055 1.058

Malic acid 1.086 1.111 0.958 1.000 1.160 1.084 0.970 1.052

Aconitic acid, cis- 1.107 1.247 1.094 0.871 0.977 1.022 0.964 0.971

Gluconic acid 1.135 0.959 0.923 0.724 0.962 1.133 0.946 1.152

Oxalic acid 1.222 1.003 0.889 0.837 0.804 0.909 0.994 0.969

Citric acid 1.048 1.085 0.834 0.799 0.917 0.991 0.970 0.778

Ethanolamine 0.981 0.966 0.921 0.907 0.871 0.941 1.094 0.884

Glutamic acid 1.125 0.883 0.806 1.020 0.896 0.894 0.896 0.794

Phosphoric acid 1.214 1.068 0.858 1.114 0.983 0.924 0.866 0.908

Glucose-6-phosphate 1.213 0.913 0.914 1.029 0.840 1.161 0.852 0.899

Fructose-6-phosphate 1.142 0.881 0.892 1.047 0.849 1.189 0.828 0.937

Quinic acid 1.108 1.049 1.082 0.821 0.814 1.107 0.827 0.896

Shikimic acid 1.176 0.983 0.943 0.801 0.902 1.029 0.784 0.994

Sucrose 1.134 0.979 0.954 0.865 0.813 0.970 0.806 0.951

Inositol, myo- 0.902 1.134 0.938 0.713 1.022 0.877 1.150 0.831

Pyridine, 2-hydroxy- 1.117 1.158 1.180 0.807 0.696 0.788 1.043 0.983

Fructose 0.939 1.216 0.957 0.941 0.824 1.057 1.099 1.128

Glucose 0.909 1.152 0.985 0.953 0.919 1.004 1.120 1.050

Kestose, 1- 0.966 1.136 0.915 0.818 0.774 0.945 0.881 1.181

Suppl. Table 4, Metabolite profiling based on gas chromatography:mass spetroscopy. Data are ratios HOSUT10 grains:wildtype grains. Values in bold, significant differences (P < 0.1, in italics and P < 0.05 upright).Supplemental Table SIII Metabolite profiling, based on gas chromatography:mass spectroscopy. Data are ratios

HOSUT10 grains:wildtype grains, values in bold, significant differences (P < 0.1, in italics and P < 0.05 upright).