C H R O M A T O G R A P H YP
rod
uct S
pe
cificatio
ns
Thermo Scientific Dionex UltiMate 3000 Diode Array and Multiple-Wavelength Detectors Fast, sensitive, and rugged UV-Vis detection
Integrating hardware, software and separation chemistry, Thermo Fisher Scientific offers UHPLC to everyone – for all needs – establishing the new standard in conventional LC.
The Thermo Scientific™ Dionex™ UltiMate™ DAD 3000 detector is a high-resolution, 1024-element diode array detector (DAD) available in Rapid Separation (200 Hz) and Standard (100 Hz) versions. It operates with the Thermo Scientific™ Dionex™ Chromeleon™ Chromatography Data System software to provide a variety of spectra views, including 3-D plotting and automated chromatogram handling. The high resolution and low-noise performance of the DAD-3000 family makes it ideal for the most sensitive and accurate library searches and peak purity analyses.
The detector is also available as a multiple wavelength detector (MWD) in Standard (100 Hz) and Rapid Separation (200 Hz) versions.
• Datacollectionatupto200Hzusingamaximumofeightsingle-wavelengthdatachannelsandone3-Dfield(3-DonlywithDAD-3000(RS))forbestsupportofultrafastseparations
• Standardversionsoperateatupto100Hzdatacollectionrateforoptimumsupportof62MPa(9000psi)UltiMate3000Standardsystems
• Accuratecompoundconfirmationwitha1024-element,highresolutionphotodiodearray
• FlexibilityinbothUVandVisapplicationswith190–800nmwavelengthrange
• Low-noiseoverthefullspectralrangeusingdeuteriumandtungstenlamps
• Fastandaccuratewavelengthverificationusingabuilt-inholmiumoxidefilter
• ThedetectorcanbeupgradedwiththeThermoScientific™Dionex™UltiMate™3000pHandConductivityMonitor(PCM-3000)foraccuratemonitoringpHgradients
• Excellentreliabilityandreproducibilitywithlowbaselinedrift(typically<500µAU/h)
• Simplifiedroutinemaintenancewithfrontaccesstopre-alignedcellsandlamps
• IDchipsonflowcellsandlampsforidentificationandlife-spanmonitoring
• ChromeleonCDSsoftwareforfullcontrolandflexibledatahandling
• Front-paneldisplayforeasymonitoringofdetectorstatustomaximizeuptime
• Flowcellsforsemi-micro,semi-analytical,analytical,andsemi-preparativeapplications
• Flowcellsavailableinstainlesssteelandbiocompatibleversions
2 Table1.DAD-3000/MWD-3000designfeaturesandhighlights
DAD-3000/MWD-3000 Series Design Features and Highlights Their Benefit to You
Deuteriumandtungstenlampssupportawidedetectionwavelengthrangefrom190nmto800nm.
Goodsignal-to-noiseratioacrossthewavelengthrange.Highapplicationflexibility.
A1024-elementphotodiodearrayoptimizesspectralresolution. Accuratecompoundconfirmation.
Uptoeightchannelsofsingle-wavelengthdatacollection.Simultaneously,theDADcanacquire3-Ddata.
Simultaneousquantitationunderindividually-optimizedconditions.
Upto200Hzdatacollectionrate,including3-Ddata(underChromeleon7.1softwarecontrol).
Bestchromatographicseparationefficiencyevenwithultrafastseparations.
Excellentlinearity,typicallyupto2.0AU.Combinesdrugassayandimpurityprofilinginpharmaceuticalanalysiswithnoneedtoreinjectatadifferentdilution.
VariableslitforRapidSeparationversionsoftheDADandtheMWD.Achievebestspectralresolutionwiththenarrowslitsetting,improvenoiseperformancewiththewideslitsetting.
Flowcellrangecoverssemi-micro,semi-analytical,analytical,andsemi-preparativeflowrateapplications,instainlesssteelandbiocompatibleversions.
Flowcelleasilyadaptabletothecolumnformatinuse.
UpgradeanMWDtoaDADfor3-DUVfieldacquisitionsupport.Splityourinvestmentindiodearraytechnology.Purchasetheinstrumentyouneedtodayandadaptittoyourfutureapplicationneedslater.
Fastandtool-freeexchangeoflampsandflowcellsfromthedetectorfront.
Maintenancetasksareeasytodo.
LampsandflowcellsequippedwithIDchipsforidentification(e.g.,typeandserialnumber)andlifespanmonitoring.
HistoryistraceableevenwhentheflowcellorlampisplacedinanotherDAD-3000orMWD-3000seriesmodule.
Chromeleonsoftwaremonitorsthelifespanofthelampsandalertsuserswhentochangethem.
Enablesuserstoplanaheadforrequiredmaintenanceincreasingoverallproductivity.
Qualificationperiodissavedinthemodule.Avoidstheuseofunqualifiedequipment,evenwhenthemoduleisswitchedtoanothersystem.
PowerfulChromeleonsoftwaretoolsallowimmediateon-linereviewof3-Ddataandextractionofsinglewavelengthchannelsfromthe3-Dfield.Thesoftware’sadvancedsearchandpeakpurityalgorithmshelpyoutoquicklycreatespectralibrariesandidentifyunknowncompoundsaswellasimpurities.
Makesiteasy,fast,andconvenienttoextracttheinformationyouneed.
PCM-3000upgradeforpHandconductivitymonitoring.ThecombinationofUV-VisdetectorandPCM-3000isidealformonitoringsalt-orpH-gradientseparationsfore.g.monoclonalantibodiesorotherbiomolecules.
3Design OverviewTheDAD-3000opticalbenchdesignincludestwolightsources–deuteriumandtungstenlamp–togetherwith1024diodesandpowerfulelectronics(Figure1).Polychromaticlightpassesthroughtheflowcell.Thedispersedwavelengthsaresimultaneouslydetectedbythediodearray.Thisensuresthesamesensitivityforsingle-andmulti-wavelengthoperation,providingconvenient,optimized,andsensitivequantificationofmaincompoundsandrelatedimpurities.
Reliable QuantificationNoiselevelswhenusingthewideslitare±8 µAUwithatypicalbaselinedriftof<0.5 mAU/hat254nm(flowingmobilephase).Thelownoiseandbaselinedriftallowfordetectionofweaksignals(goodsignal/noiseratio)andconsistent,reproducibleintegration.Atypicaldetectorlinearityupto2.0AUshowscorrelationcoefficientsof>0.999andaresidualstandarddeviationbelow5%withcaffeine.Thewidelinearityrangeallowsreliablequantificationofprimaryandsideproductsinthesamerun.
Sensitivity and Spectral ResolutionWhenacquiringspectraldataforpeakidentificationand/orpeakpurityanalysis,highresolutionspectraareessential.Atthesametime,itisimportanttoobtaingoodsignal-to-noiseratiosfordetectionofweaksignals.TheDAD-3000’sfamilyelectronicsaredesignedtoprovidehighspectralresolutionincombinationwithlownoise.
Figure2showsanexampleofanexplosiveresidueanalysis.Onesamplewasautomaticallyinjectedontwocolumnsofvaryingselectivity.Targetanalyteswereconfirmedbyspectralmatch,evenatverylowconcentrations.Chromeleon’scustomizablereportingfunctionsprovideinstantaneousaccesstoresults,listconfirmedorunconfirmedtargetanalytes,quantifythesubstances,anddefinetheirpeakpurity.
Figure2.DAD-3000examplefollowingEPAmethod8330forexplosivesresidues.Acustomizedreporttakesresultsfrombothseparationsandvisualizesifacompoundisconfirmedandpure.
Figure1.Lightfromthetungstenlampisfocusedthroughanapertureinthedeuterimlamp.Thecombinedlightthenpassesthroughtheflowcellanddiffractiongratingtothediodearray.
Holmium OxideFilter 1024-Element
Diode Array
Flow Cell
Deuterium Lamp
Tungsten Lamp
Grating
Variable Slit*
*Only available on DAD-3000RS and MWD-3000RS
–1
15
mAU
2
7
89
10 11
12
13
14
254 nm
0 5 10 15 20 25 30 35 40–1
15
mAU
Minutes
2
7
10
12
13
14
8 9
254 nm
Contaminated soil sampleThermo Scientific™ Acclaim™ Explosives Column E1
Contaminated soil sampleAcclaim Explosives Column E2
4 Rapid Separation LC DetectorsAtypicalpeakrequires30 datapointsforaccurateandpreciseintegration.Up-to-dateUHPLCseparationscanresultinverynarrowanalytepeaks.Thesecanevenreachwidthsofonly0.25s,requiringmorethan100Hzdatacollectionrate.
TheRapidSeparationdiodearraydetectorseasilymeetthisrequirementwithdatacollectionratesofupto200Hz.Thisdetectorperformanceprovideshighlyaccurate,preciseintegrationandretentiontimerecognitionforanyseparationchallenge.
Figure3showshowdetectorswithnon-sufficientdatacollectionratescompromisethepeakintegration.Peakareaprecisionandretentiontimerecognitionareadverselyaffectedatthesametime.
UV Spectra AcquisitionTheDAD-3000RSprovidesuptoeightsingledatachannelsandfullspectralscansat200Hzforeventhemostchallengingapplications.Figure4showstheseparationof10analytes,uracilandninealkylphenones,in10seconds.Thefullspectralscancaneasilykeepupwiththisspeed.PeakidentificationandpeakpurityanalysisareasconvenientandpowerfulasforconventionalLCseparations.Combiningthesefeatureswiththelownoisespecification,theDAD-3000RSisidealforultrafastanalysesandreadytomeetfuturechallenges.
Optimize for Best Sensitivity or Spectral ResolutionBothrapidseparationversions,theDAD-3000RSandtheMWD-3000RS,provideavariableslitattheentranceofthespectrograph.Thisisaneffectivetooltofurtheradaptthedetectortotheapplicationrequirements.Anarrowslitprovidesthehighestspectralresolution,resolvingevenveryfineabsorbancespectra,suchasforpyrene(Figure5).However,UVabsorbancebandsaretypically30nmandwider.Inthesecases,abroadslitresultsinthehighestdetectionsensitivity.Morelightpassesthroughthespectrograph,reducingbothshort-termnoiseandthelimitofdetection.
Figure3.UltrahighdatacollectionratesperfectlysupporttheintegrationofthesharpestUHPLCpeaksforhighestprecisionofquantificationandretentiontime.
Figure4.200HzUVspectraacquisitioneasilysupportstheseparationof10peaksin10seconds.
Figure5.ThevariableslitoftheDAD-3000RSandtheMWD-3000RSisatooltooptimizeforbestspectralresolutionorbestdetectionsensitivity.
0
10
Seconds
0
60
190
Typically 30% noise reduction
400
%
nm
Narrow Slit
Wide Slit
200 Hz 100 Hz 50 Hz 20 Hz
0.25 seconds
5Rapid Multiple Channel DetectionTheRapidSeparationversionoftheMultipleWavelengthDetectoroffershighspeedandsensitivityforbothsingleandmultiplewavelengthdataacquisition.Itsdiodearraytechnologyachievesmulti-channeldetectionofuptoeightchannelswithoutmovingparts.
Regardlesshowmanywavelengthsarerecorded,thedetectorcanmeasureallwavelengthsatthesamehighsensitivityandatamaximumdatacollectionrateof200Hz.Up-to-datevariablewavelengthdetectorsachievemultiplewavelengthdetectionbyrapidlyswitchingbetweenalternatewavelengths,butthisadverselyaffectsshort-termnoiseandreducesthemaximumdatacollectionratesignificantly.TheMWDdoesnothavethislimitation.
Upgrade MWD to Full DAD FunctionalityMWDdetectorsarebasedonphotodiodearraytechnology.TheonlydifferencefromDADdetectorsisthattheMWDdoesnotsupporttheacquisitionof3-Dspectra.
These3-Dspectracapabilitiesarefield-upgradable,turningyourMWDintoafull-blownDAD.Youmaythereforestaggeryourinvestmentindiodearraytechnology,purchasetheinstrumentyouneedtoday,andadaptittoyourfutureapplicationneedslater.
Ergonomic Design and Advanced System MonitoringAllfluidicpartsoftheMWDandDADdetectorseriescanbeaccesseddirectlyfromthefront.Thedoorfoldsdown,servingasaminiaturelabbenchtoplacetoolsandpartsduringmaintenance(Figure7).
Thedeuteriumandtungstenlampsofthedetectorcanalsobeaccessedfromthefrontbyopeningthefingertightnutsofthelampcover.Changingtheselampsisequallyfastandeasy.ModificationsofflowcellsorlampsareautomaticallytrackedbyintegratedIDchips.AllrelevantinformationisstoredinthelampandmoveswiththedetectorifitistransferredtoanotherLCsystem.Chromeleonconstantlymonitorstheseandvariousothercounterstoprovideyouwithearlywarningswhenapartisdueforreplacement.Inaddition,ChromeleonsoftwaremonitorsandverifiestheinstrumentqualificationstatustomeetyourGLPcompliancegoals(Figure8).
Figure7.Ergonomicinternalfrontdesign(afterremovalofflowcell/lampscovers):directaccessto1)theD2andtungstenlamps,2)theflowcell,and3)theflowcelladapterblockforeasyoperationandmaintenance.
Figure6.Sub-1minsoftdrinkanalysiswithsimultaneousacquisitionofeightdifferentUVchannels,measuredwiththeMWD-3000RS.
Figure8.DAD-3000automatedqualificationmonitoring.
1
2
3
0 10 20 30 40
87
65
43
21
Seconds
6 Easy Screening with Chromeleon Spectral LibrariesChromeleonsoftwaresupportseasycreationofspectrallibrariesofknowncompounds(Figure10).
Spectraofunknownpeakscanbecomparedautomaticallyagainstallspectrainalllibraries.Chromeleonsoftwarewillreporttheclosestmatches.Thelibrarycanincludefields,suchassolventcompositionandwavelengthresolution,toprovidemoreadvancedsearchcriteria.
Fast Access to Data and Results via the Peak Purity Analysis ViewViewalldatainasinglesummarywindow,usingadisplaystyleofyourchoice(Figure9).DragtheverticallinetoviewtheassociatedUVspectraatdifferentpointsofthechromatogram.Dragthehorizontallinetoseetheassociatedchromatogramfortheselectedwavelength.Thismakesiteasytofindthebestsignalresponse.Infact,Chromeleonsoftwarecanevenperformthistaskforyouautomatically.Afterthat,itisasimplesteptoextracteitherthechromatogramorthespectrumyouneed.
Getting the Most from Your Spectral Data – with EaseAtypicaldiodearray3-Dfieldcontainsavastamountofinformation.Effectivetoolsareneededtoworkwithsuchvolumesofdata.PowerfulChromeleonsoftwareputsthesetoolsatyourfingertipsandmakesiteasytoextracttheinformationyouneed.WiththeDAD-3000family,youcan:
• Reviewyourresultsinasinglewindow
• Extractsingle-wavelengthchannelsfromyour3-Dfield
• Quicklycreatespectralibrariesforknowncompounds
• Automaticallyidentifyunknownpeaksbasedonasearchofoneormorespectrallibraries
• Easilyidentifypeaksthatcontainunderlyingimpurities,usingadvancedpeakpurityalgorithms
• Effectivelyreporttheresultsinaformatofyourchoice
Figure9.Convenientextractionofasinglewavelength(250nm)from3-DfielddataofaUHPLCseparation.
Figure10.Spectrallibraryscreening.
7Assess Specificity in Seconds with Peak Purity IndexThePeakPurityIndexcanbeoverlaidwithachromatogramforstraightforwardidentificationofpeaksthathaveanunderlyingimpurity(Figure11).
Apurepeakhasaconsistentpeakpurityindex.Anydeviationsindicateanunderlyingimpurity.Thisat-a-glancetechnologycanspeedmethoddevelopmentandhelptoensurespecificity.
Application FlexibilityFourdifferentflowcellsizesareavailablefortheDAD/MWD-3000series:
• AnalyticalflowcellssuitableforHPLCandsomeUHPLCapplications
• Semi-microflowcellsforbestchromatographicresolutionwithsmallIDcolumnsandnarrowanalytepeaks
• Thesemi-analyticalflowcelldesignedfor3mmIDcolumnseparations
• A0.4mmopticalpathlengthsemi-preparativeflowcellforUVdetectionofhigh-concentrationanalytes
Thiswiderangeofflowcellsmeansyoucanadaptyoursystemeasilytoyouranalyticalneeds.
Accurate pH and Conductivity MonitoringThePCM-3000isanupgradeoftheDAD-3000andMWD-3000seriesforaccurateon-linepHandconductivitymonitoring.ThecombinationofUV-ViswiththePCM-3000isidealforpHorsaltgradiention-exchangechromatographyofcompounds,e.g.monoclonalantibodies,conductivitymonitoringingradientseparationsofbiomolecules,andthereforeagreatadditiontotheThermoScientific™Dionex™UltiMate™3000BioRSsystems.
Figure11.Peakpurityexamples.
Figure12.FlowcelldesignofUltiMate3000DiodeArrayDetectors.
Part Number Description Volume (µL)
Optical Path Length (mm)
6082.0100 AnalyticalFlowCell,StainlessSteel 13.0 10.0
6082.0200Semi-AnalyticalFlowCell,StainlessSteel
5.0 7.0
6082.0300 Semi-MicroFlowCell,StainlessSteel 2.5 7.0
6082.0400 AnalyticalFlowCell,PEEK™ 13.0 10.0
6082.0500 Semi-MicroFlowCell,PEEK 2.5 7.0
6082.0600 Semi-PreparativeFlowCell,PEEK 0.7 0.4
Table2.DAD-3000andMWD-3000flowcelloptions
Figure13.ThePCM-3000isahighperformance,easy-to-use,andcosteffectivesolutionforpHandconductivitymonitoring.
7.5 9.0
Minutes
Straight lineindicates purepeaks
7.5
9.0
Minutes
Indicates underlyingco-elutingpeak
Australia+61397574486Austria+43810282206Belgium+3253734241Brazil+551137315140China+85224283282Denmark+4570236260
Finland+358932910200France+33160924800Germany+4961034081014India+912267429494Italy+3902950591Japan+81668851213
Korea+82234208600Netherlands+31765795555Norway+46855646800Singapore+6562891190Sweden+46855646800Switzerland+41617167700
Taiwan+886287516655UK/Ireland+441442233555USA and Canada+8472957500
Thermo Fisher Scientific, Sunnyvale, CA USA is ISO 9001:2013 Certified.
www.thermoscientific.com/dionex©2013ThermoFisherScientificInc.Allrightsreserved.PEEKisatrademarkofVictrexPLC.ISOisatrademarkoftheInternationalStandardsOrganization.PEEKisatrademarkofVictrexPLC.AllothertrademarksarethepropertyofThermoFisherScientificInc.anditssubsidiaries.Specifications,termsandpricingaresubjecttochange.Notallproductsareavailableinallcountries.Pleaseconsultyourlocalsalesrepresentativefordetails.
Pro
du
ct Sp
ecifica
tion
s
PS70286_E07/13S
Specifications
Dimensions(h×w×d) 16×42×51cm(6.3×16.5×20in.)
DetectionType Single-beam,reverse-opticsdesignwithconcaveholographicgrating
MaximumData 200Hz:DAD-3000RS(including3-Dacquisition)CollectionRate) andMWD-3000RS(underChromeleon7.1software
control)100Hz:DAD-3000(including3-Dacquisition)andMWD3000
WavelengthRange 190to800nm
Noise Wideslit:<±8μAUat254nm.Narrowslit:<±10μAUat254nmresponsetime2s(accordingtoASTMtimeconstant~1s),4nmbandwidth,waterat1.0mL/min
Drift <1mAU/h(typically<0.5mAU/h)at254and520nmwithwaterat1.0mL/min
Linearity <3%RSDandcorr.coeff.>0.9995upto1.5AU,typically<5%RSDandcorr.coeff.>0.999upto2.0AU
LightSource Deuteriumlamp,tungstenlamp,temperaturecontrolforbothlamps
WavelengthAccuracy ±1.0nm,self-calibrationwithD-alphaline,verificationwithholmiumoxidefilter
WavelengthPrecision ±0.1nm
PixelResolution <1nm
SlitWidth Narroworwideslit,settablefortheDAD-3000RSandMWD-3000RS
PCConnection AllfunctionscontrollableviaUSB2.0;integratedUSBhubwiththreeUSB2.0ports
GLPFeatures AutomaticInstrumentQualification(AutoQ),SystemWellnessandQualificationMonitoringwithChromeleonsoftware,lampandcellIDchips
UserInput/Display LCDindicatingsystemparameters,standbybutton,threeLEDsforstatusmonitoring,fourfunctionkeysforinitialoperationandmaintenance
PowerRequirements 85-260V,50/60Hz,max.150W
Ordering Information
ToorderintheU.S.,call1-800-346-6390,orcontacttheThermoFisherScientificofficenearestyou.OutsidetheU.S.,orderthroughyourlocalThermoFisherScientificofficeordistributor.Refertothefollowingpartnumbers.
Detectors Part Number
DAD-3000RSRapidSeparationDiodeArrayDetector 5082.0020(withoutflowcell)
DAD-3000DiodeArrayDetector(withoutflowcell) 5082.0010
MWD-3000RSRapidSeparationMultipleWavelengthDetector 5082.0040(withoutflowcell)
MWD-3000MultipleWavelengthDetector(withoutflowcell) 5082.0030
Accessories Part Number
Analyticalflowcell,stainlesssteel,13μL 6082.0100
Semi-analyticalflowcell,stainlesssteel,5μL 6082.0200
Semi-microflowcell,stainlesssteel,2.5μL 6082.0300
Analyticalflowcell,PEEK,13μL 6082.0400
Semi-microflowcell,PEEK,2.5μL 6082.0500
Semi-preparativeflowcell,PEEK,0.7μL 6082.0600
UpgradeMWD-3000RStoDAD-3000RS 6082.3045
UpgradeMWD-3000toDAD-3000 6082.3035
PCM-3000forDAD-3000orMWD-3000Series 6082.2000(ShippedwithapHandconductivityflowcellandapHelectrode)
Deuteriumlamp 6074.1110
Tungstenlamp 6074.2000