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ISOLATION OF ANTIBIOTIC-PRODUCING
MICROORGANISMS
T.Monirah Alhammadi
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There are several ways to isolate microbes
that produce antibiotics from the soil. Below are some methods used to isolate microbes produce antibiotics effective against some microbes
PRINCIPLE
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Random Isolation in pure
cultur
crowded-plate
Catrer and lockwood method
Isolation of Antagonistic soil microorganisms
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#soil isolation agar plate
pure culture test each culture against Plant Pathogenic
microbes inhibition zone # Demonstrates the effectiveness of
the microbe isolated from the soil against the microbe pathological
1-Random Isolation in pure cultur
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1-Random Isolation in pure cultur
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#fungus pathological pollination
growth media Incubate plates
#Then sprinkle dishes of soil dilution 1 \ 1000 to 1 \
100000 approximately 0.6 cm Incubate plates
Tset the plate inhibition zone
2 -Catrer and lockwood method
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Catrer and lockwood method
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]In this experiment , we will use the
crowded-plate technigue for isolation of antibiotic-producing microorganisms from soil
3-crowded-plate
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#Soil suspensions(1:500 dilution of soil
sample suspension) #cultures of E.coli, S.aureus, (P.aeruginosa
#test tube #petri dishes
#1-ml and 5-ml pipettes #bunsen burner
#sterile water
Materials
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1 -label three test tubes 1,2and3. with apipette , add 5ml of tap water to eash tube.
2 -shake the provided 1:500 soil sample thoroughly for 5 minutes to effect a uniform soil-water suspension.
3 -using a 5-ml pipette , transfer 5 ml from the 1:500 dilution to tube 1 and mix . The final dilution is 1:1000
4-using another pipette , transfer 5 ml from tube 1 to tube 2 and mix.
The final dilution is 1:20005-using another pipette , transfer 5 ml from tube 2
to tube 3 and mix. The final dilution is 1:4000
Methods:
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.6-Using separate 1-ml pipettes , transfer 1 ml of the 1:1000, 1:2000
Dilutions to their appropriately labeled petri dilutions
agar ) trypticase soy ) 7- pour one tube of molten
8 -incubate all plates in an inverted position for 2 to 4 days at 25C
Methods:
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THE END