Physical Sample Management Training
Typeofsamples
RockOutcrop
SoilCore
Coral
RockCore
Module:RockOutcrop
EarthCube – iSamples Sample Management Training Module
EarthCube – iSamples Sample Management Training Module
RockOutcrop Collec1on Processing Management
PartIRockOutcropSampleCollec:on
EarthCube – iSamples Sample Management Training Module
RockOutcrop Collec1on Processing Management
RockOutcropSampleCollec:onOutlineofthissec:on:
I. Ra1onaleforsampling• Whatareyourfieldobjec1ves?• Whatabout“old”samples?
II. Protocolforsamplecollec1on• Collec1ngthesample• Collec1ngtherightinforma1on• Incorpora1ngexis1ngsamples
(“previouslycollected”)
III. ProtocolforSampleInforma1onSynthesis• PreparingforIGSNRegistra1on
EarthCube – iSamples Sample Management Training Module
RockOutcrop Collec1on Processing Management
I.Ra:onaleforsampling:“FieldBased”metamorphicpetrologyandtectonicsstudiesgo
throughseveralstagesofplanningbeforefieldworkcommences.Thereareseveraltypesoffieldcampaignsthatyieldsamples:
1. ReconnaissanceFieldSampling
2. TargetedFieldSampling
3. Acquisi1onofsamplesfromcollaborators/othersources(fieldworkdonebyothers).
EarthCube – iSamples Sample Management Training Module
RockOutcrop Collec1on Processing Management
ReconnaissanceFieldSampling: unmappedorlarge-scalemappedareas
• Samplestoberepresenta1veofdifferentlithologies,• maybemixedintermsofcollec1onpurposeandtypeof
deriva1vedata• Collec1onismoreforcoverageandregionalgeologic
interpreta1on,ortorepresentimportantfieldrela1onships.Forexample,igneousintrusionsmaybesampled,unconformablesedimentaryrocks,andperhapsmetamorphicrocksofdifferentprotoliths/composi;ons,thoughthesecouldbelessusefulinstudiesofspecificmetamorphicprocessescondi;ons.
EarthCube – iSamples Sample Management Training Module
RockOutcrop Collec1on Processing Management
ReconnaissanceFieldSampling: Alargevaria1onofsampledensityandsizeofasuiteispossibleinsuchascenario,oZenreflec1ng:(1) costsofsampleprocessing(2) payloadconstraints(3) viabilityofperformingusefulanalyses(4) personalpreference/philosophy
• Itispossible/likelythat“targeted”fieldsamplingtypestudycanbeperformedorderivedfromasubsetofsuchareconnaissancemapping/survey.Ifsamplesuitesize/densitypermi]edsamplinglithologiesdeemedusefulforspecificpurposes(keyfieldrela1onshipsorrepresenta1vecomposi1on,mineralogy,metamorphicgrade)meaningthistypeoffieldcampaigncanservemul1plepurposes.
EarthCube – iSamples Sample Management Training Module
RockOutcrop Collec1on Processing Management
Targetedfieldsamplingofpreviouslymappedareas:• Thistypeoffieldsurveytypicallyisfocusedonanareathathas
previouslyhaddetailedmappingandlikelysamplingperformed• Sampledensityislikelyhighlyvariablebecausespecific
lithologiesorfieldrela1onshipsaretargetedandsampledingreaterdensity
• Sampledensitys1llmustdependinparton:(1) costsofsampleprocessing(2) payloadconstraints(3) viabilityofperformingusefulanalyses(4) personalpreference/philosophy• Thissamplingmaybecombinedwith
detailedmappingatveryfinescalestorepresentfieldrela1onships.
EarthCube – iSamples Sample Management Training Module
RockOutcrop Collec1on Processing Management
Studentconsidera:ons:Iden1fythetypeoffieldworktheyareperformingandconsiderwhysamplesaretobecollected.• Willtherebeaspecificques1onguidingfieldworkorwillamore
broadques1onofregionalgeologybethefocus?• Thismaybethebestwayofconsideringappropriatesample
density,andbasicallyhowtobudget1meinthefield—amajorchallengefornew(andexperienced)geologists.
EarthCube – iSamples Sample Management Training Module
RockOutcrop Collec1on Processing Management
Studentconsidera:onsforundergraduatestudents:• itislikelythatfieldworkisinpartalearningexperience
whereac1veresearchiscombinedwitheduca1onallearningac1vi1es:learningtomakemeasurements,sketches,interpreta1ons,etc.
• Inthissitua1on,itisimportanttobeawareofwhataspectsofthefieldworkaremostcri1caltotheresearchprojectanditsgoals/purpose.
EarthCube – iSamples Sample Management Training Module
RockOutcrop Collec1on Processing Management
II.ProtocolforSampling:1. Chooseasamplenamingprotocolforyourownorganiza1on.
Consideranamingschemethatmakessensetoothers.Thisislargelyapersonalpreference,butitisworthplanningahead.
2. Iden1fythesamplingsite.Keyconsidera1onsinclude:a. Coverage:areyouinaloca1onthat
wouldhavesignificanceinyourstudyarea?
b. Rocktype:Canyoureasonablyiden1fywhatyouwillsampleandisitappropriateforfurtheranalysis(isthereanintendedpurpose)?
c. Availabilityofasample:canyouactuallycollectanin-placesamplehere?
EarthCube – iSamples Sample Management Training Module
RockOutcrop Collec1on Processing Management
ProtocolforSampling:(con:nued)3.Recordnotesaboutwhere,bywhom,andwhensamplingwasperformed.• Alsohelpfulisinforma1onaboutotherfeaturesthatwouldaid
someonetofindthisexactsite(trees,paths,roadnames,buildings,etc.)
• UseaGPSforthespecificsamplingloca1on(within1meter).RecordLa1tude/Longitudeinnotes(inaddi1ontoyourGPSdevice--Itisbesttohaveawri]enbackup).
• Measureandrecordanystructuraldatatoassociatewiththesample.
EarthCube – iSamples Sample Management Training Module
RockOutcrop Collec1on Processing Management
ProtocolforSampling:(con:nued)4.Photographthesamplesite,keepingnotesaboutthefilenameforthephotos.Besuretoincludearepresenta1onofscale.Ifyouhaveaddi1onalphotos/observa1onsfromthesamesiteyousample,recordtheseaswellinthesameplace.
Bluerockhammeris~20inchesinlength.
EarthCube – iSamples Sample Management Training Module
RockOutcrop Collec1on Processing Management
ProtocolforSampling:(con:nued)5.Ifyouareinterestedinmicrostructures,followstepstocollectanorientedsample.6.Carefullybreakoffanintactrocksample.Assesswhethertheremovedsamplemeetsyourcriteriaforaviablesample.Ifpossible,returnthesampletoitsoriginalsite/orienta1onandphotographitinplace.Thisispar1cularlyimportantifthesamplerepresentsanykeyfieldrela1onships(youmaynotknowatthispoint,soallthebe]ertobeprepared).
EarthCube – iSamples Sample Management Training Module
RockOutcrop Collec1on Processing Management
Awordaboutfloatsampling:Floatistobeavoidedasonecan’tbecertainitisinplace.! Theonly1mestoconsiderfloatsamplingwouldbe:
• Floatexactlymatchestheadjacentoutcropintermsoflithologyandyouwouldnotbeabletosampletheoutcropforotherreasons.
• Floatrockspresentanopportunitytostudyaprocessthatisnotrepresentedinoutcrop,andisdecoupledfromregional/localgeology.
• Floatrepresentstheonly“exposed”rockinanareaofsignificantreliefthatwasnotlikelyglaciatedormodifiedbyhumans.
EarthCube – iSamples Sample Management Training Module
RockOutcrop Collec1on Processing Management
ProtocolforSampling:(con:nued)7.Labelthesamplewithyourchosensamplenumber,in2ormoreplacesusingablueormetallicsharpie(thesearemorevisibleonearth-tonedrocks,especiallywhenwet—avoidblacksharpie).8.Modifyyoursamplingnotestoreflectthesamplesyoucollectandactuallykeep.IfyoudiscardsamplesaZernamingthem,donotgobacktofillinnumbersinyournamingscheme(therewillalwaysbesamplesthatdonotsurvivethroughcollec1on,processing,archive,etc.)Thiswayyourincrementalsamplenumberschemewillbeachronologicalorderaswell.
EarthCube – iSamples Sample Management Training Module
RockOutcrop Collec1on Processing Management
ProtocolforSampling:(con:nued)PreviouslyCollectedSamplesAcquisi1onfromotherInves1gatororCollaborator• Fieldworkperformedinthepast,samplessharedwith
documenta1on• Sampleinforma1onmaybethorough/completeormore
likelypar1al(duetodifferentsamplingprotocolormanagementphilosophy)
ProtocolforIncorpora:ngsampleseffec:velyintoworkflow:• Gatherallsampleinforma1on.Considerwhatispreserved/
availableandwhatmaytakeeffort/1metoobtain.• ArethesamplesalreadyregisteredwithIGSN’s?Ifso,request
anddownloadsampleinforma1on.• Otherwise,preparesampleinforma1onforuploadtocreate
IGSN’sforthesesamples.
EarthCube – iSamples Sample Management Training Module
RockOutcrop Collec1on Processing Management
III.ProtocolforSampleInforma:onSynthesis:1.Beginningwithyoursampleinforma1onspreadsheetfromthefield,copytheworksheettoanewsheetandmodifythecolumnheaderstobereadbytheIGSNsystem.Besurethefollowingcolumnsareincluded(somerequiredbySESAR):
SampleNameCollectorLa1tudeLongitudeCollec1onDateCommentRockType
"fieldname(informalclassifica1on)State/ProvinceLocalityLoca1onDescrip1onCountry
MetamorphicGrade"Comment
PresentSampleLoca1on"CurrentArchive
AddnecessarycolumnsforIGSN(consulttheSESARQuickGuide):Naviga1onType=GPSSub-ObjectType=SpecimenCollec1ondateprecision=dayClassifica1on=MetamorphicMaterial=Rock
EarthCube – iSamples Sample Management Training Module
RockOutcrop Collec1on Processing Management
III.ProtocolforSampleInforma:onSynthesis:2.DownloadaBatchRegistra1onTemplatefromSESAR,whichcontainsnecessaryformamng.Pasteallinforma1onintoSESARforma]edspreadsheetforupload.• Choosepublicsamples• Giveyourselfaweekbeforesamplesappearinsearches• Choosethefieldsaslistedabove
Note:Ifsampleinforma1onistobeuploadedtoanotherdatabasesystemsuchasMetPetDB,consideracommonformatforconsistentsamplemetadata.
EarthCube – iSamples Sample Management Training Module
RockOutcrop Collec1on Processing Management
III.ProtocolforSampleInforma:onSynthesis:Checkwithyoursupervisorastowhichaccountwillbetheownerofthesampleinforma1on.Obtainaloginfortheowneraccountorclarifywhetheryoushoulduseapersonallogin(andperhapstransferownershiptoyoursupervisor—seeSESARdocumenta1on).3.Withacompleted/savedbatchuploadfile,logintotheappropriateSESARaccount.4.Correctanyerrorsinthepreviewsystemanddownloadtheupdatedfiletoremaincurrent.Makethisfileaworksheetinthespreadsheetofyoursampleinforma1onandfielddata.
EarthCube – iSamples Sample Management Training Module
RockOutcrop Collec1on Processing Management
III.ProtocolforSampleInforma:onSynthesis:5.OnceIGSN’sareassignedforeachsample,updatethefieldsampledataspreadsheetforthisbatchwithIGSN’s.6.PrintQRCodes1ckersfortheregisteredsamplesfromSESAR.7.Preparethesampledataspreadsheettouploadsampleinforma1on(includingIGSN)toMetPetDB,thedatabaseforMetamorphicPetrology:www.metpetdb.rpi.edu8.Forinforma1onaboutpreparingsampledataforMetPetDB,seetheuserhelpinforma1on.
EarthCube – iSamples Sample Management Training Module
RockOutcrop Collec1on Processing Management
""PuJngNewSamplesawayinthelab:IGSNandMetPetDBregisteredsamplesshouldnext:• moveontothesampleprocessing(ThinSec1ons)step.
or
• bestoredinthesamplecabinetswithpreparedziplockbagsandIGSNQRcodes1ckersortags.
IGSN: BWH000001Name: EH01AKA: Collection Start Date:2009-07-09
EarthCube – iSamples Sample Management Training Module
RockOutcrop Collec1on Processing Management
PartIIRockOutcropSampleProcessing
EarthCube – iSamples Sample Management Training Module
RockOutcropSampleProcessingOutlineofthissec:on:
I. Ra1onaleforprocessing• Whatareyourobjec1ves?• Whatwillbedonewithyoursamples?
II. Protocolforsampleprocessing• Preparingthesampleforthinsec1ons• Preparingthesampleforrockchiporgrain
moun1ng• Preparingthesampleformineralseparates
RockOutcrop Collec1on Processing Management
EarthCube – iSamples Sample Management Training Module
I.Ra:onaleforsampleprocessing:Samplesacquired/collectedinthefieldshouldbecategorizedby
thetypeofdatatobeacquiredfromthem.Beforebeginningsampleprocessing,samplesshouldbepriori1zedas1meandcostconstraintsoncontractedexternallabworkmaybelimi1ng.
1. PreparingThinSec1ons
2. Preparingchip/grainmounts
3. Preparingmineralseparatestoeventuallybemountedforanalysis.
RockOutcrop Collec1on Processing Management
EarthCube – iSamples Sample Management Training Module
RockOutcrop Collec1on Processing Management
II.ProtocolforSampleProcessing:ThinSec:ons• Samplestobeprocessedforthinsec1onsareremovedfromthesamplecabinetsandplacedontherockcart.
1. Beforecumngsamples,preparealabeledziplockbagwithsamplenumberandits’IGSNs1ckera]ached.
• Putallderiva1vepiecesofsampleinthisbagunlesstheyarethinsec1ons,mounts,orseparates.
IGSN: BWH000001Name: EH01AKA: Collection Start Date:2009-07-09
EarthCube – iSamples Sample Management Training Module
RockOutcrop Collec1on Processing Management
Processingrockoutcropsamples:ThinSec:ons• Chooseathin-sec1onorienta1onforcumngaslabfromyoursample.Typicallyalinea1on-parallel,folia1on-normalcutispreferred.2. Drawtheplanarintersec1onalong
thesurfaceofyoursample.Besureyouaremakingaslargeaslabaspossible.
3. Cutslabusingauto-feedingsawor1lesaw.
4. Soakinsoapywatertoremoveanycumngoil.
5. Dryinwarmoven(~60ºC).
EarthCube – iSamples Sample Management Training Module
RockOutcrop Collec1on Processing Management
Processingrockoutcropsamples:ThinSec:ons
6. Scanwetslabonflatbedscanner,ataminimumof600dpi.• Becarefulwithwateronthe
scanner,dabanyexcesswaterandcleantheglassplatenaZeruse.
7. Chooseandmarkthinsec1onloca1on.Documentthisloca1ononslabscanimages.
Detailedslabscan.Scalebaris2cm
EarthCube – iSamples Sample Management Training Module
RockOutcrop Collec1on Processing Management
Processingrockoutcropsamples:ThinSec:ons
8. Trimslabtodesiredbilletforthinsec1on.SavealltrimmedpiecestoreturntothesamplebagwithIGSNlabel.
Slabisreadyfortrimmingtobillet.
9. Labeldrybilletonbacksidewithblue
Sharpie.Usethinsec1onnamingconven1on:“SampleNumber”+a,b,c…
EarthCube – iSamples Sample Management Training Module
RockOutcrop Collec1on Processing Management
PartIIIRockOutcropSampleManagement
EarthCube – iSamples Sample Management Training Module
RockOutcrop Collec1on Processing Management
Processingrockoutcropsamples:ThinSec:ons
10. Choosesetofbilletsforthinsec1onprocessing.Compilealistonathinsec1ons(tracking)worksheetinyoursamplemanagementspreadsheetfileforthissamplesuite.
11. Carefullywrapbillets,boxthemwithalotofbubblewrap,andshipoutforthinsec1onprepara1on.
EarthCube – iSamples Sample Management Training Module
RockOutcropSampleManagementOutlineofthissec:on:
I. Ra1onaleforsamplemanagement• Whatareyourobjec1ves?• Howwillsamplesandsampleinforma1onbe
properlymanaged?
II. Protocolforsamplemanagement• SampleAc:vityCodes• Storageofthinsec1onsandothersample
materialforinac1vesuites• Localarchivingofinac1vesamplesuites• Samplesfromthecollec1onthatareoff-site
RockOutcrop Collec1on Processing Management
EarthCube – iSamples Sample Management Training Module
I. Ra:onaleforsamplemanagementStatementofPhilosophy:
Physical samples are the basis for field-based geologic and petrologic study. To the investigator, samples represent the ability to make laboratory observations that are impossible in situ, and they should be treated as the sensitive “currency” of geology.
Time and effort managing a growing collection of samples means preserving this currency, and investing in its potential in the future. As much as scientific inquiry is built upon the past discoveries in our field, our investigative power is greatly strengthened by being able to return to the original source of our detailed analyses—our samples. With the swift advance of analytical power it should be clear that the future holds new ways of exploring information stored in our sample collections.
Samples collected as part of publicly funded research belong, in part to the public, and therefore should be ultimately available.
RockOutcrop Collec1on Processing Management
EarthCube – iSamples Sample Management Training Module
I. Ra:onaleforsamplemanagement:
Whatareyoursamplemanagementobjec1ves?• Helpourownresearchteam/collaboratorsbemoreefficientin
dealingwithsamples,fromcollec1on,throughanalysis,publica1on,andarchiving
• Makeoursamplecollec1onaccessible.• Trackandlocatesamplesandsubsamples(onsiteandoffsite)at
differentstagesofuse.• Ul1mately,topreservetheusefulnessofagivensample,
par1cularlyforapplica1onsthatmaynothavebeenconsidered.
RockOutcrop Collec1on Processing Management
EarthCube – iSamples Sample Management Training Module
I. Ra:onaleforsamplemanagement:
Howwillsamplesandsampleinforma1onbeproperlymanaged?• AsamplemanagementprotocolispresentedforRockOutcrop
samples.• Samplesenteringthelabworkflowwillfollowtheprotocolsfor• Samplecollec1onanddocumenta1on• Sampleprocessing(preparingforandperforminganalysis)• Samplemanagement(storing,trackingandarchiving)
RockOutcrop Collec1on Processing Management
EarthCube – iSamples Sample Management Training Module
II.ProtocolforSampleManagementAllsamplesinthelabaremanagedwithansimpleac1vitycode.Ac:vityCodes:1. Cut2. Examine3. Analyze4. Store5. Archive6. Teaching7. Out
Ac1vitycodingforprocessedsamplescanbetrackedontheIGSNQRcodeprintoutstoredwiththehandsample.
RockOutcrop Collec1on Processing Management
EarthCube – iSamples Sample Management Training Module
II.ProtocolforSampleManagement
Ac1vityCodeExplana1ons:• Cut:SamplesareintheprocessofbeingregisteredwithIGSN’s
andcuttopreparethinsec1onsorothersubsamples.Thisincludessampleswithslabsbeingscannedoranysamplenotyethavingathinsec1on.
RockOutcrop Collec1on Processing Management
EarthCube – iSamples Sample Management Training Module
Ac1vityCodeExplana1ons:• Examine:Samplesnowhaveatleastonethinsec1onprepared
andarebeingexaminedpetrographicallybuthavenothadanyanalysesperformed.• Thesesamplesexistas(1)thecutpiecesofthehand
sampleorcore,plus(2)athinsec1on(s)storedinlabthinsec1onboxeswiththeircollectedbatch.
RockOutcrop Collec1on Processing Management
RR14-12a
EarthCube – iSamples Sample Management Training Module
Ac1vityCodeExplana1ons:• Analyze:Sampleshaveatleastonethinsec1onpreparedand
arebeingorhavebeenanalyzedbygeochemicaltechniques(e.g.electronmicroprobe,SEM,LA-ICPMS).
RockOutcrop Collec1on Processing Management
• Polishedthinsec1onsmaybecarboncoatedandshouldbestoredwithotherAnalyzeSamplesthataretobebroughttotheexternallab.
• Analyzesamplesmaybearrangedbysuitebutareseparatedfromthefullsuitesatthisstageforanalysis
EarthCube – iSamples Sample Management Training Module
Ac1vityCodeExplana1ons:• Store:WorkisconsideredcompleteonStoresamples.• Thinsec1onsarecompiledforthecompletesuite,including
sec1onsthatwereanalyzed.• Thesamplematerialisinventoriedandcondensedinthe
labeleddrawersinthestoragecabinets.• Storageisconsideredlong-termun1larchivingisnecessary
becauseofspaceissues.
RockOutcrop Collec1on Processing Management
EarthCube – iSamples Sample Management Training Module
Ac1vityCodeExplana1ons:• Archivesamplesaretobeboxedandmovedfromstorage
cabinetstoalongtermloca1on.• Thesesamplesarecollec1onsmanagedbythelocal
department.ThePIisnolongerac1velydoingresearch.• Archivesamplesshallberemainlabeledandbaggedjustas
Storesamplesare,butwillbeboxedassuites.• Boxedsuitesshouldincludeasampleinventorylistand
printedsheetofIGSNQRcodes.
RockOutcrop Collec1on Processing Management
EarthCube – iSamples Sample Management Training Module
Ac1vityCodeExplana1ons:• Teachingsamplesaretreatedandstoredseparatelyfromother
samples,inteachingcollec1onsunless• Thesesamplesarecollec1onsmanagedbythelocal
department.Theyshouldnotbemanagedbyanyonebutinstructors.
• TeachingsamplesmayservetwopurposesandmayalsobeArchiveorStoresamplesremovedfromtheirrespec1vesuitesandmanagedasTeachingsamples.Inthiscase,specialnotesshouldbemadeintheArchiveorStoresampleinventories.
RockOutcrop Collec1on Processing Management
EarthCube – iSamples Sample Management Training Module
Ac1vityCodeExplana1ons:• Outsamplesarenotpresentlyin
thecollec1onsthoughtheyares1llconsideredpartoftheirrespec1vesuites.SamplesmaybesentOutfordifferentreasons,butshouldbe:• Listedasoutonthewhiteboard
iftheycomefromExamineorAnalyzesuites.
• NotedonsampleinventorysheetsiftheyareoutfromStoreorArchivesuites.
RockOutcrop Collec1on Processing Management