Use of biostimulants for a sustainable horticulture:
mitigation of thermal and salt stress in tomato
Phase 1: Leibniz- Institut für Gemüse und Zierpflanzenbau Großbeeren/Erfurt
Researcher: IGZ Supervisor:Tiziano Celli Dr. D. Schwarz
Tuscia University Supervisor:Dr. G.Colla
Index presentation
• Introduction: “Torno Subito 2015”, project “Use of biostimulants for a sustainable horticulture”
• Materials and Methods:– Biostimulants general introduction– Trainer (LISIVEG technology)– Hydrolysates Protein and cold tolerance– Effectiveness of TRAINER in lettuce and tomato– Materials and methods experiment IGZ
• Acknowledgement
Torno Subito 2015• Regione Lazio improving
knowledge and professionality of young citizens- two parts
• My case: experiment carried out in tomato with partnership IGZ Großbeeren/Universita’ della Tuscia
• Improving the tomato cultivation practice in Lazio Region (specifically Latina’s province)
Biostimulant
Any substance or microorganism applied to plants or the rhizosphere:1. Enhance/benefit nutrient uptake, use and
efficiency 2. Enhance quality traits 3. Tolerance to abiotic stresses 4. Furthermore important:• Mixtures of such substances and/or microorganisms
• Action regardless nutrients content. Du Jardin 2015, Scientia Horticulturae 196 (2015): 3-14
…With the aim to achieve a (more) sustainable agriculture
• Feeding a growing population.
• Extreme meteorological events
• Facilitating nutrient assimilation and use
• Products quality attributes
• Improve soil health
Classification of biostimulants• Humic and fulvic acids• Protein hydrolysates and
other N-containing compounds
• Seaweed extract and botanicals
• Chitosan and other biopolymers
• Inorganic compounds (Si, Al, Se)
• Beneficial fungi and bacteria (biofertilizer)
Protein hydrolysates and other N-containing compounds
• Definition: Mixture of polypeptidized, oligopeptidized and aminoacids that are manufactured from protein sources using partial hydrolysis.
• Origin: Animal or vegetal protein, chemical or enzymatic hydrolyzation• Main function: growth, nutrient uptake, tolerance to abiotic stresses,
product quality• Weakness: Animal for vegan and Islamic and Hindus population,
biological production• Strength: Better efficiency of the plant, higher production, higher
secondary methabolites content, waste disposal (animal)
HP biostimulants and cold tolerance • Heavier fruits• Improving of the cold
tolerance and less damage under frost conditions
• Unclear mechanism of action of the biostimulant to enhance the tolerance
Bogunovic et alt. Hort.Sci. 42, 2015 (3): 132-140
Previous experiments with TRAINER
• Hormone like action (Auxin, gibberellin)• Accelerate metabolic pathway N (NR,
GS)• Bigger root apparatus High N uptake
• Reduce loss of production under salt stress (Proline)
• Higher photochemical activity (Antioxidant)• Better nutritional status (N,P)• Depending on the way of application
Lucini L. et alt., Sc. Hort. 182 (2015): 124-133 Colla G. et alt., Front. in plant Sc. (2014) Vol. 5:, Art. 448, 1-5
Trainer (Italpollina)• TRAINER is based on 100%
vegetal aminoacids and peptides
• LISIVEG® innovative treatment technology preserves weak amino-acids (ex. Tryptophan) and maintains their activity unaltered.
• Ammynoacids in Levorotatory form (selective hydrolization)
Hypotheses
Hypothesis 1- Different varieties will react differently under cold stress at the use of the plant biostimulant.Hypothesis 2- Sensitivity to cold stress is alleviated by using of biostimulants due to a greater uptake efficiency, and use, of nutrientsHypothesis 3- The low nutrient supply for the plant in the scarce solution will be compensated by greater root surface caused by the application of the biostimulant
Material and Methods • NFT system • 2 different temperatures-(15 ˚C and 20 ˚C)• 2 different varieties-Komett and Super
Sweet• 2 different nutrient concentration of the
nutrient solution- 0.7 dSm¯² and 3.5 dSm¯² • Trainer/Control
Continuous measurements and operations
• First treatment with biostimulant dipping the plant
• Biometric measures (number of leaves, lenghtLeaf area, number of trusses)
• Photosynthesis gas exchange measurement
• Nutrient solution uptake• Other routine operations:
change of the nutrient solution in the 0.7 dSm¯², daily check EC and pH in the tanks, suckers removal , polinization, Treatments with biostimulant each 10 days dosis 3ml/l)
Additional measurements harvest• Yield (green fruits)• FM, DM of shoots and
roots• Leaf sample 5th leaf for
analyses of nitrate reductase (NR) and glutamine synthetase (GS)
• Sugars and starches in the leaf
• Root morphology (Scan)• Mineral analysis: NO3, NH4,
total N, P, K, Ca, Mg, Mo, B, Fe, Mn, Cu, B.
Danke für Ihre Aufmerksamkeit
Acknowledgement
• Regione Lazio and Torno Subito 2015• Dr. D. Schwarz • Prof. Dr. G. Colla• Prof. Dr. Youssef Rouphael• Frau Gundula Aust• Thomas Runge, Christian Jorgiz, Oskar Lindner, Joss
F. Kurz• All the other technical assistant for the kind support