dr. carmen e. rexach microbiology 1 spring 2013 1/pdf micro... · 1) medical microbiology, kayser,...

14
Dr. Carmen E. Rexach 1 Microbiology 1 SPRING 2013 Instructor: Dr. Carmen E. Rexach webpage: http://instruction2.mtsac.edu/crexach email: [email protected] office: 60-2109 phone: (909)274-4223 office hours: TBA and by appointment Lecture : M/W 11:30-12:55 Rm 11-2312 Lab : M/W 1:15-4:25 Rm 60-2506 Course Description : Microbiology 1 covers the fundamental concepts of microbiology with emphasis on bacteria. Main aspects of the course are surveys of microbial classification, morphology, physiology and genetics; beneficial and pathological aspects; growth and control of microbes; virology, immunology, and host-microbe interactions. Important infectious diseases of humans are discussed. Laboratory exercises examine microbial morphology, physiology and genetics, as well as environmental influences of microorganisms. Laboratory techniques include culturing, examining, and identifying microorganisms. Prerequisites : Chem 10 or Chem 40. The Chem 50/51 sequence is preferred for biology and most pre- health professions majors. (Please check with the transfer institution to determine the correct course(s) for your major). Required textbooks: 1) Medical Microbiology, Kayser, FH, et al, Theime publishers 2) A Photographic Atlas for the Microbiology Laboratory, Michael LeBoffe and Burton Pierce 3) Laboratory Manual, Dr. Carmen E. Rexach, 2011 (this is a revised edition) 4) Micro 1 Reader ( will be handed out in class) 5) Control of Communicable diseases, current edition Recommended 1) A good medical dictionary, such as Taber’s. Additional required materials and supplies: 1) 20 #882 Scantrons (due by Wed, March 7th) 2) Lab coat (must be long sleeved, knee length, and must close in front) 3) Gloves 4) Safety goggles 5) Closed toed shoes that completely cover the top of the foot 6) 3 unopened packages of Petri dishes (due by Wed, March 7th) 7) 3 ring binder 8) Laboratory journal (must be purchased from bookstore ) 9) Fine point permanent marker 10) #2 pencils 11) A good eraser 12) Clear millimeter ruler 13) Calculator 14) Slide box Lecture format : Lecture outline is available online at http://instruction2.mtsac.edu/crexach in pdf format and can be printed out at science computer center. Please review slides for upcoming lectures prior to lecture whenever possible. These are intended to provide an overview of the lecture and serve as a guide for assigned reading. Although the lecture will be presented using PowerPoint, the lecture itself will be interactive and students are expected to prepare in order to participate. Group activities, problem solving, animations, and hands-on activities will also be used in order to engage students in active learning.

Upload: others

Post on 29-May-2020

3 views

Category:

Documents


0 download

TRANSCRIPT

Dr. Carmen E. Rexach

1

Microbiology 1

SPRING 2013

Instructor: Dr. Carmen E. Rexach webpage: http://instruction2.mtsac.edu/crexach email: [email protected] office: 60-2109

phone: (909)274-4223 office hours: TBA and by appointment

Lecture: M/W 11:30-12:55 Rm 11-2312

Lab: M/W 1:15-4:25 Rm 60-2506

Course Description: Microbiology 1 covers the fundamental concepts of microbiology with emphasis on bacteria. Main aspects of the course are surveys of microbial classification, morphology,

physiology and genetics; beneficial and pathological aspects; growth and control of microbes; virology, immunology, and host-microbe interactions. Important infectious diseases of humans

are discussed. Laboratory exercises examine microbial morphology, physiology and genetics, as

well as environmental influences of microorganisms. Laboratory techniques include culturing, examining, and identifying microorganisms.

Prerequisites: Chem 10 or Chem 40. The Chem 50/51 sequence is preferred for biology and most pre-

health professions majors. (Please check with the transfer institution to determine the correct course(s) for your major).

Required textbooks:

1) Medical Microbiology, Kayser, FH, et al, Theime publishers

2) A Photographic Atlas for the Microbiology Laboratory, Michael LeBoffe and Burton Pierce 3) Laboratory Manual, Dr. Carmen E. Rexach, 2011 (this is a revised edition)

4) Micro 1 Reader (will be handed out in class)

5) Control of Communicable diseases, current edition

Recommended 1) A good medical dictionary, such as Taber’s.

Additional required materials and supplies: 1) 20 #882 Scantrons (due by Wed, March 7th)

2) Lab coat (must be long sleeved, knee length, and must close in front) 3) Gloves

4) Safety goggles 5) Closed toed shoes that completely cover the top of the foot

6) 3 unopened packages of Petri dishes (due by Wed, March 7th)

7) 3 ring binder 8) Laboratory journal (must be purchased from bookstore)

9) Fine point permanent marker 10) #2 pencils

11) A good eraser

12) Clear millimeter ruler 13) Calculator

14) Slide box

Lecture format: Lecture outline is available online at http://instruction2.mtsac.edu/crexach in pdf format

and can be printed out at science computer center. Please review slides for upcoming lectures prior to lecture whenever possible. These are intended to provide an overview of the lecture and

serve as a guide for assigned reading. Although the lecture will be presented using PowerPoint, the lecture itself will be interactive and students are expected to prepare in order to participate.

Group activities, problem solving, animations, and hands-on activities will also be used in order to engage students in active learning.

Dr. Carmen E. Rexach

2

Exams and Quizzes: Lecture Exams: There will be 3 lecture exams during the semester. The lecture exams will be

multi-format (essay, short answer, multiple choice, matching, etc.) and will be worth 120 points each. Each lecture exam will cover a specific unit, but will require an understanding of previous

information (this course builds on your understanding of concepts….you cannot “erase your disc”

after each exam!)

Pre-final: To help you prepare for the final, I will give a mini-final in class the week before finals week. It will be in the same format as the final (all multiple choice), and will be worth 120 points

(60 multiple choice questions). This test will not count against you, but if you do better on the pre-final than on one of the previous 3 lecture exams, you may use the score

to replace the lower grade.

Lecture final: The lecture final is the only exam I give each semester that is all multiple choice.

This exam is worth two lecture exams (120 x 2 = 240 pts) and is comprehensive. The study guide for the lecture final is already posted on the class website and is a great place to start

NOW preparing for the final exam.

Lab Quizzes: During the semester you will be given 12 lab quizzes worth 15 points each as

indicated on the attached lab schedule. The lowest lab quiz score will be dropped, so that the total number of points possible is 165 (11 x 15). The lab quizzes are given at the beginning of

lab and cannot be made up.

Lab Challenges: Your skills in lab will be tested by various methods, including a pure culture

challenge and the identification of two unknowns, one strictly by morphology and staining, the other using biochemical methods.

Lab Journal: You are expected to record your work in a lab journal as described in your lab

manual. The lab journals are available at the bookstore. Each lab journal will be evaluated

during the semester according to the rubric in your lab manual. Evaluation dates will be announced in class. PLEASE SEE LAB JOURNAL EXPECTATIONS AND GRADING RUBRIC.

Pathogen Challenge: Case studies are presented and you are asked to identify the disease and

the causative agent by scientific name. To assist you, you may use the pathogen sheets that you

will be preparing throughout the semester. The pathogen list for Micro 1 is posted on the website, and the pathogen sheets are also posted and can be printed. Every pathogen list

includes all pathogens that are included in the lecture, plus additional pathogens that we do not cover in class. It is your responsibility to complete the pathogen sheets in a timely fashion as no

other tool will be permitted for the pathogen challenge. All pathogen sheets must be your own work and information is restricted to each space and page as indicated. No photographs may be

included, however, students may draw images in the space provided. The pathogen challenge is

worth 50 points.

Lab Practicum/Lab final: The lab final is a practicum and involves identifying organisms, specific types of techniques, and results of experiments utilized during the semester. It is comprehensive

and also includes a written portion.

Case studies: Case studies will be included with each lecture exam. These will be drawn from the

pathogen sheets assigned up to the date of each exam. The case studies will be administered separately during the test period and students will be allowed to use their prepared pathogen

sheets for the case studies only. This portion of the exam will be timed.

Journal Club: Scientific information is reported in peer reviewed journals. It is often difficult for students

to keep up with all of this new information by reading each of these articles and doing the necessary research to understand what is being reported. Therefore, colleges and universities

hold journal clubs once a week during which students present articles to their fellow students and

Dr. Carmen E. Rexach

3

professors. Each student will choose an article; obtain approval from the instructor, then

research and report on the article in a power point presentation to the group. Additional information on criteria for the presentation and a grading rubric will be provided in class. The

journal club presentation is worth a maximum of 50 points. PLEASE NOTE: ALL STUDENTS MUST PARTICIPATE IN THIS ACTIVITY! IT IS YOUR RESPONSIBILITY TO SIGN UP

FOR A DATE AND TIME IN ADVANCE THAT FITS WITH YOUR SCHEDULE. SPECIAL

ARRANGEMENTS CAN ONLY BE MADE IN ADVANCE FOR OTHER TIMES WITH WRITTEN PERMISSION FROM YOUR INSTRUCTOR AND VERIFICATION THAT THERE

IS A CONFLICT WITH ALL AVAILABLE JOURNAL CLUB TIMES AND DATES. This assignment cannot be made up in any other way.

Evaluation and Grading Policies: The grade students have earned will be computed as follows:

Lecture grade (52% of course total):

Three lecture exams at 120 pts each 360 pts. Journal Club presentation 50 pts

Pathogen Challenge 50pts Comprehensive final lecture exam 240 pts.

Lecture total: 700 pts

Lab grade (48% of course total): Twelve Lab quizzes (14 pts each) 165 pts

Unknown #1 40 pts Unknown #2 60 pts

Lab Journal 100 pts Report Lab 33 40 pts

Lab Practicum (lab final) 120 pts

Lab total: 525 pts

Total pts 1225

Attendance Policy: Attendance in class and in lab is crucial to your success. You are expected to attend

every class on time! If you are tardy or absent, please do not disturb the class to find out where we are in the lecture, or what you have missed. It is your responsibility to approach other

students after class and obtain missing material. Quizzes are given at the beginning of class and cannot be made up. Exams are scheduled and cannot be made up, except under extremely

compelling circumstances with documentation!

Registration policies (adds and drops): If there is space to add students, these students must

bring proof of “adding” to the next class meeting in order to begin attending the class. If you decide to drop the class, it is your responsibility to go to the office and drop. If

you stop attending and do not drop, you will be given the grade you earned, which is usually an F. This grade remains on your transcript even if you retake the class in the future. Please don’t

put yourself into this position!! Drop deadlines are available on line and in your class schedule.

Grades will be based on the following:

A ≥ 90% 1102-1225

B ≥ 80% 980-1101

C ≥ 70% 857-979 D ≥ 60% 735-856

F < 60% <735

Rules, rules, and more rules:

1. I do not accept late assignments. 2. Safety is a priority in lab. You are expected to follow all rules and regulations and to behave in a

responsible manner.

Dr. Carmen E. Rexach

4

3. You are responsible for the equipment you use in lab. Use it with care.

4. NO TEXT MESSAGING IN MY CLASS! 5. Be courteous and respectful to each other. Do not carry on conversations with each other during

lecture. It prevents other students from hearing what is being said. Do not interrupt lecture if you come in late. Do not answer your cell phone during lecture.

6. We will be working with pathogens and with organisms that are not pathogenic under ordinary

conditions, but could be pathogenic to those who have certain medical conditions. Please alert me to any potential condition that might affect your participation in lab. I will try to ensure your

safety as much as possible! 7. Students arriving in lab without proper protective equipment (lab coat, safety goggles, closed

toed shoes, gloves) will not be allowed to participate and will be asked to leave the lab. 8. Your attendance is determined by your signature on the attendance sheet. If you forget to sign,

you weren’t there!

9. I will not accept any handwritten reports or case studies! Computers are available for all students in the computer lab and they have very generous hours to accommodate any schedule.

If you do not have a computer available at home, please visit the computer lab asap so that you can determine the optimal time for you to use the facilities.

10. Scheduled field trips are part of the course. If you do not attend, you are counted as

absent. 11. Remember that your performance in class is directly correlated with the amount of time you

spend in lab and in study. Do not schedule appointments during lab on the assumption that you will finish early!

12. The most common cause of failure in this course is procrastination. Keep up with your work. 13. I consider cheating a form of theft. I will not tolerate it. Please read the academic integrity

policy below and the Mt SAC Biology Department Policy on Student Cheating attached to this

syllabus. I will hold you to these documents and will act in accordance with these policies.

Academic Integrity Policy: As stated in the Mt. San Antonio College catalog “Honesty is primarily the responsibility of each student. The College considers cheating to be a voluntary act for which

there may be a reason, but for which there is no acceptable excuse. It is important to

understand that collaborative learning is considered cheating unless specifically allowed for by the professor.” The term cheating "… includes but is not limited to plagiarism, receiving or

knowingly supplying unauthorized information, using unauthorized material or sources, changing an answer after work has been graded and presenting it as improperly graded, illegally accessing

confidential information through a computer, taking an examination for another student or

having another student take an examination for you, and forging or altering grade documents". In any act of academic dishonesty, the student will automatically receive a zero on that test or

assignment and the incident will be reported to the Dean of Students Services, Director of Student Life, Director of Admissions and Records, and the Dean of Natural Sciences. In the case

of more egregious offenses, a grade of "F" in the course may be assigned (regardless of the student's average) as per College and department policies (refer to Mt SAC College Catalog for

further information).

Remember that you are ultimately responsible for your success in class! Review your lecture outlines

each day before class, complete the assigned reading before you come to lecture, spend an adequate amount of time studying (about 3 hours outside of class per hour of lecture), attend all

study sessions, and ask lots of questions!! Microbiology is lots of fun!

Dr. Carmen E. Rexach

5

Score Sheet: Please record all of your scores on this sheet as your personal record. Remember that

one of the labs will be dropped (lowest score). Keep all returned papers/exams until the end of the semester should there be any question about your scores.

EXAM POINTS

POSSIBLE

YOUR SCORE YOUR % GRADE

Lecture Ex #1 120

Lecture Ex #2 120

Lecture Ex #3 120

Journal Club 50

Lab quiz #1 15

Lab quiz #2 15

Lab quiz #3 15

Lab quiz #4 15

Lab quiz #5 15

Lab quiz #6 15

Lab quiz #7 15

Lab quiz #8 15

Lab quiz #9 15

Lab quiz #10 15

Lab quiz #11 15

Lab quiz #12 15

Report lab 33 40

Lecture Final 240

Unknown #1 40

Unknown #2 60

Pathogen challenge 50

Lab journal 100

Lab Practicum Final 120

Extra credit

Grand total

**The instructor reserves the right to amend the assignments/exams in this syllabus as needed.

Dr. Carmen E. Rexach

6

Micro 1 Lecture Schedule Spring 2013

Date Lecture Topic

2/25 Introduction to Microbiology 1

2/27 Introduction to Microbiology, history

3/4 Chemical principles and microscopy

3/6 Cell Structure: Prokaryotic vs. Eukaryotic cells

3/11 Cell Structure: Prokaryotic vs. Eukaryotic cells

3/13 Prokaryotic vs. Eukaryotic cells, Classification of microorganism

3/18 The Prokaryotes: Domains Bacteria and Archae

3/20 The Prokaryotes: Domains Bacteria and Archaea

3/25 The Prokaryotes: Domains Bacteria and Archaea

3/27 Introduction to Viruses, Viroids and Prions

4/1 Viruses, Viroids, Prions

4/3 Lecture Exam 1

4/8 Microbial Growth

4/10 The Control of Microbial Growth

4/15 Antimicrobial Drugs

4/17 The Eukaryotes: Fungi, Protozoa, Helminths

4/22 The Eukaryotes: Fungi, Protozoa, Helminths

4/24 Vectors

4/26 7TH ANNUAL HEALTH PROFESSIONS CONFERENCE

4/29 Microbial Metabolism

5/1 Microbial Metabolism

5/6 Lecture Exam 2

5/8 Microbial Genetics, Biotechnology and bioremediation

5/13 Immunology and Vaccinology

5/15 Microbial Mechanisms of Pathogenicity

5/20 Principles of Disease and Epidemiology (Case studies handed out)

5/22 Bioterrorism

5/27 HOLIDAY…NO CLASSES Outbreak analysis (case studies)

5/29 Emerging Infectious Diseases (Case studies due)

6/3 Lecture Exam 3

6/5 Prefinal

6/10 Final Exam 10:30am-1pm; Lab exam review

**Schedule is subject to adjustment or amendment at the discretion of the instructor.

Dr. Carmen E. Rexach

7

Micro 1 Lab Schedule Spring 2013

Week Date Lab# Topic Quizzes

1 2/25 1,2,3 Orientation/Introduction/You’ve got WHAT?, Microscopy, Calibration

2/27 3,4,5,6 Metric system, Aseptic Technique, Preparing smears, simple stain & negative stain

2 3/4 7 Differential stains: Gram stain #1 (labs 1,2)

3/6 8 Differential stains: Acid fast stain

3 3/11 9,10 Spore stain and capsule stain

3/13 11 Motility: hanging drop, flagella stain & motility agar; intro to Bergey’s #2 (labs 4-7)

4 3/18 Morphological unknown day # 1 #3 (labs 8-10)

3/20 Eval 1 Morphological unknown day # 2

5 3/25 Field Trip #1: City of Hope

3/27 12 Eukaryotic Microbes: Mycology I #4 (labs 11)

6 4/1 13 Eukaryotic microbes: Mycology II: Biobrew 102* Field Trip #2: Bootleg Brewery

4/3 14 Eukaryotic microbes: Parasitology I (protozoa)

7 4/8 15 Eukaryotic microbes: Parasitology II (worms) #5 (labs 12-14)

4/10 17(pt I) 19,22 Pure culture techniques: streak plate and pour plate isolation methods, stock cultures, bacterial population counts

8 4/15 20,21 Bacterial growth characteristics: oxygen requirements (cultivation of anaerobes), temp, pH & microbial growth

#6 (lb 15,17,19)

4/17 23,24 Control of microbial growth: osmotic pressure, effects of uv light, oligodynamic action, Evaluation of antiseptics & alcohol, antimicrobial sensitivity testing (Kirby-Bauer test)

#7 (labs 20,21,22)

9 4/22 16,17(pt 2),18 Creating a dichotomous key (beginning of unknown #2 eval), Media preparation.

4/24 25 Selective and differential media: blood agar, desoxycholate, phenol alcohol agar

4/26 7th Annual Health Professions Conference @ Mt. SAC

10 4/29 26, 27 Hydrolysis of starch, lipids, proteins, indole, urease, & phenylalanine tests

#8 (labs16,17,18)

5/1 28 Biochemical assays: oxidation & fermentation tests #9 (labs 23-25)

11 5/6 29 Biochemical assays: multiple test media

5/8 Eval 2 Unknown 2: Read out and completion of identification Overview of outbreak investigation

#10 (labs 26, 27)

12 5/13 30, 31,32 (pt I) Biotechnology: Introduction, Bacterial transformation Biotechnology: Restriction Enzyme Analysis of DNA (part I)

#11 (labs 28, 29)

5/15 32, cont 33

Biotechnology : Restriction Enzyme Analysis of DNA (part II) Begin outbreak investigation

13 5/20 33 Outbreak investigation #12 (lab 30-32)

5/22 33 Outbreak investigation

14 5/27 HOLIDAY…NO SCHOOL!

5/29 33 Outbreak investigation

15 6/3 Pathogen test (REPORT DUE)

6/5 Lab Practicum

16 6/10 Final exam and lab test review

Dr. Carmen E. Rexach

8

Microbiology Lab Journal Instructions

General information:

Your lab journal is a detailed, accurate, unabridged record of all of the work that you do in lab during the

semester. The key words are DETAILED, ACCURATE, and UNABRIDGED!

1) DETAILED: Absolutely everything you do in lab should be recorded in your lab journal (please see the

formatting notes below for specific information). This includes the purpose of your lab, any changes to the published lab protocol, and all of your results, including a detailed written description as well as

drawings and tables, your conclusions based on your results, and a discussion of how your results refer back to the original purpose of the lab.

2) ACCURATE: You must write exactly what you observe in your lab manual. You are not supposed to write what you think you should observe.

3) UNABRIDGED: If you make a mistake, draw a line through it. NEVER ERASE OR WHITE OUT

ANYTHING IN YOUR LAB JOURNAL! Sometimes what you think was wrong turns out to be right, or

provides important information that you can use for a future experiment. If you draw a line through it, it indicates that you think it is an error, but it is still there for you to read.

Remember: Anyone else should be able to pick up your journal and reproduce the experiment that you

conducted…and hopefully get the same, or similar, results. GET IN THE HABIT OF NOT WRITING ANYTHING IN YOUR LAB MANUAL….WRITE EVERYTHING IN YOUR LAB JOURNAL!

Lab Journal Format:

1. The required lab journal for Micro 1 is available in the Mt SAC Book Rac. It is bound and contains a blue flexible front and back cover. The pages are white graph paper that is already numbered.

2. Write your name and contact information on the inside front cover. Since all of these lab manuals look exactly alike, they can easily get confused. Please also write your name in black indelible ink

(Sharpee) on the upper right hand corner of the outside of the front cover.

3. When you open your lab journal, you will find preprinted page numbers in the Table of Contents.

Please complete the Table of Contents as you go so that labs can be easily located.

4. Because it is so important to have a complete record of everything you do, and also important to have a permanent formal record of the lab, the journal is divided into two parts.

5. The right facing pages are used for the formal right up only. The left facing pages are for notes! DO

NOT WRITE ANYTHING ON THE LEFT SIDE THAT YOU EXPECT ME TO GRADE!

6. At the top of each page, write the lab number and complete title.

7. Write the date on the designated spot on each page of the lab write up

8. YOU MUST INCLUDE THE PAGE NUMBERS OF YOUR NOTES AT THE TOP OF EACH COMPLETED LAB WRITE UP! 9. Any images you draw must be accompanied by a descriptive paragraph. This is because our ability to

accurately represent an image in a drawing is not always that precise. A written description, therefore, adds an additional dimension to your work.

10. If your lab journal DOES NOT contain the notes on the left sided pages in addition to your formal write up, you will be deducted an automatic 25 points.

Dr. Carmen E. Rexach

9

11. If a lab is not complete (ie, purpose, m&m’s describing any exceptions or lack thereof, results including a written description of any drawings, discussion, and conclusions) it will not be graded.

The general format for each lab is as follows.

I. Purpose : Write a brief statement of purpose that describes why you are performing this lab. Please write only one purpose for each lab. If the lab has several parts, you may write several sentences for the

purpose.

II. Materials and methods: Although you want to record everything in your lab journal, it is not necessary to reproduce all of the materials and methods published in the lab manual. If you did this, it

would take up lots of space and it is not necessary, since you can find the protocols and the materials in

the manual. What you DO want to record is any changes. For example, let’s say that you are writing up Lab 6, and the published lab included E. coli and Proteus mirabilis, but you were given Proteus vulgaris and Helicobacter pylori instead. You would write: “As stated in the Micro 1 Lab Manual, Lab #6 Negative Stains, pages 37-39, for 2011-12, with the

following exceptions; Proteus vulgaris and Helicobacter pylori were used instead of E. coli and Proteus mirabilis.”

If there were no changes in the lab, you would write: “As stated in the Micro 1 Lab Manual, Lab #6 Negative Stains, pages 37-39, for 2011-12, with no

exceptions.”

III. Results : Record all of your data. You may use drawings for an observational lab, or tables to help

you organize data. Whichever you use, you must also summarize your results in a written descriptive paragraph. A table or drawings by themselves are insufficient.

IV. Discussions: Tell me what your results mean relative to your purpose. You can also include

comments about the lab in general and how it can be improved. Any suggestions for future work on this

topic?

V. Conclusions : What conclusions can you draw from your results?

Frequently Asked Questions:

1) Only the purpose should be recorded before you start the lab. Once you begin to work, record any

notes or raw data on the left facing pages. This information can then be used to complete the other sections of your formal write up at another time.

2) Don’t wait too long to complete the lab write up!! You will forget important things and this could be a

problem. Also, don’t hesitate to write down as much raw data as you need. Don’t count on your

memory, or your lab partners notes.

3) Sometimes your lab will span several days. If you need more space, just continue your formal write up on another page of your lab journal.

4) Do not fabricate data. If you are absent, you cannot make up a lab. I have read and understand the proper format for maintaining a lab journal in Micro 1.

Name ________________________________

Date __________________________________

Dr. Carmen E. Rexach

10

Lab Journal Grading Rubric

Lab # Topic Point value

1 Intro and lab safety 0

2 You’ve got what 3

3 Microscopy and aseptic technique 3

4 Calibrating the microscope 0

5 Preparing smears and simple stains 3

6 Negative stain 3

7 Gram stain 3

8 Acid fast stain 3

9 Spore stain 3

10 Capsule stain 3

11 Motility stain 3

12 Mycology I 4

13 Microbrew/field trip 5

14 Protozoa 3

15 Helminthes 3

16 Dichotomous key 5

17 Culture characteristics 3

18 Media preparation 0

19 Streak plate and pour plate isolation 3

20 Oxygen and anaerobes 3

21 Temperature and pH 3

22 Quantitative techniques 3

23 UV light and osmotic pressure 3

24 Antimicrobials 3

25 Blood agar 3

26 Hydrolysis of starch, lipids, NA 3

27 Indole production 3

28 Oxidation and Fermentation tests 3

29 Multiple test medium 3

30 Biotechnology 0

31 Transformation 3

32 Restriction enzymes 3

33 Outbreak investigation 3

FT City of Hope 5

Unk #2 Biochemical unknown 5

Total points possible 100

Dr. Carmen E. Rexach

11

Micro 1 Lecture Schedule Spring 2012

Date Lecture Topic

2/27 Introduction to Microbiology 1

2/29 Introduction to Microbiology, history

3/5 Chemical principles and microscopy

3/7 Cell Structure: Prokaryotic vs. Eukaryotic cells

3/12 Cell Structure: Prokaryotic vs. Eukaryotic cells

3/14 Prokaryotic vs. Eukaryotic cells, Classification of microorganism

3/19 The Prokaryotes: Domains Bacteria and Archae

3/21 The Prokaryotes: Domains Bacteria and Archaea

3/26 The Prokaryotes: Domains Bacteria and Archaea

3/28 Introduction to Viruses, Viroids and Prions

4/2 Viruses, Viroids, Prions

4/4 Lecture Exam 1

4/9 Microbial Growth

4/11 The Control of Microbial Growth

4/16 Antimicrobial Drugs

4/18 The Eukaryotes: Fungi, Protozoa, Helminths

4/23 The Eukaryotes: Fungi, Protozoa, Helminths

4/25 Vectors

4/27 6TH ANNUAL HEALTH PROFESSIONS CONFERENCE

4/30 Microbial Metabolism

5/2 Microbial Metabolism

5/7 Lecture Exam 2

5/9 Microbial Genetics, Biotechnology and bioremediation

5/14 Immunology and Vaccinology

5/16 Microbial Mechanisms of Pathogenicity

5/21 Principles of Disease and Epidemiology (Case studies handed out)

5/23 Bioterrorism

5/28 HOLIDAY…NO CLASSES Outbreak analysis (case studies)

5/30 Emerging Infectious Diseases (Case studies due)

6/4 Lecture Exam 3

6/6 Prefinal

6/11 Final Exam 10:30am-1pm; Lab exam review

**Schedule is subject to adjustment or amendment at the discretion of the instructor.

Dr. Carmen E. Rexach

12

Mt. San Antonio College Biological Sciences Department Policy on Student Cheating

POLICY

1. No dictionaries, reference materials, notes, or programmable calculators may be used during any exam or quiz unless authorized by the professor.

2. No electronic devices, of any type, may be used during any exam or quiz unless authorized by

the professor. a. Electronic devices include, but are not limited to: cell phones, PDAs (personal digital

assistants, earphones, cameras, MP3 players, IPods, translation devices, and electronic

dictionaries.

3. No talking, signaling, sharing of note cards, calculators or other materials is allowed during any exam or quiz.

4. Only the materials required or authorized for an exam or quiz should be taken out of your notebook, backpack, pocket, or purse. All other materials should be put away as instructed, this

includes electronic devices.

5. Students may not leave the classroom during an exam or quiz unless authorized by the professor. If a student leaves the room without permission, the test or quiz will be forfeited at that time.

6. This policy will be strictly enforced by all professors in all classes taught in the Department.

CONSEQUENCES:

7. A single act of cheating or academic dishonesty in any form may result in as much as receiving

an “F” in the course.

8. Action taken by the professor will be consistent with the college policy on cheating and academic dishonesty. In addition, a report regarding the violation will be submitted to the Director of

Student Life for further action, which may also result in further disciplinary action, including, but

not limited to suspension or expulsion from the college.

WHAT IS CHEATING?

Some examples of cheating include, but are not limited to:

a. Plagiarism, which is the use of materials authored by another person or obtained from a commercial source or the use of passages without proper acknowledgment.

b. Having or using unauthorized materials during any exam or quiz

c. Notes concealed in or written on clothing, hats, or skin (as examples).

d. Looking at another student’s work during any exam or quiz.

e. Changing answers on a returned exam in order to claim there had been a grading error.

f. Sharing any content of exams or quizzes with individuals who have not yet taken it.

g. Removing an exam or quiz from the classroom without the professor’s approval.

Dr. Carmen E. Rexach

13

h. Taking photos of exams, quizzes, completed ScanTrons®, or exam keys.

i. Turning in work that was generated by other individuals or by the same individual but in a prior semester, including but not limited to: lab report data, lab report or homework

questions, homework assignments, and extra credit assignments.

j. Working together on a lab experiment when told to work individually.

k. Falsifying lab data.

l. Allowing another student to look at your exam or quiz, or allowing another student to

copy your homework, lab reports, or other assignments. (If that work is duplicated you may also receive the same penalties listed above for violation of the biology department

policy on cheating, and the college policy on cheating and academic dishonest.)

m. Falsifying documents, including signatures.

If you are unclear about what constitutes cheating in your class or for a particular assignment, please

contact your instructor for clarification before the assignment is due.

Sign the Student Acknowledgment form and return it to you professor.

Keep this policy for records.

Dr. Carmen E. Rexach

14

Mt. San Antonio College

Biological Sciences Department Policy on Student Cheating Student Acknowledgement

I have received, read, and understand the Biological Sciences Department Policy on Student

Cheating.

____________________________________ _________________________________

Professor Course Title

________________________________________

Print Last Name, First Name

________________________________________ ____________________ Signature Date