Dr. Marisa N. Fernandes

BiologicalBiologicalAreaArea

Researches in the Dept of Physiology Sciences covers most areas of Biology

Molecular BiologyNeurobiologyEndocrinologyPhysiology of ExerciseZoophysiology and Comparative Biochemistry

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Prof. Dr. Marisa Narciso FernandesProf. Dr. Francisco Tadeu RantinProf. Dr. Ana Lúcia Kalinin

Topics covered

Studies on the adaptation of aquatic animals to environmental changes, pollutants and stress conditions.

Prof. Dr. Francisco Tadeu Rantin Prof. Dr. Ana Lúcia Kalinin

Cardiac physiology

In vitro

excitation-contraction coupling and contractility of ventricle stripes after changes in temperature, O2 e pH, Ca2+ availability

In vivo

Heart frequency (fH ) and electrocardiogram characterization

The research cover in vivo and in vitro studies

Prof. Dr. Marisa Narciso Fernandes Prof. Dr. Francisco Tadeu Rantin Prof. Dr. Ana Lúcia Kalinin

Respiratory physiology and Nervous control

The research covers:

respiratory responses of fish and other vertebrates to environmental variables such as dissolved oxygen, pH, temperature and pollutants

Prof. Dr. Marisa Narciso Fernandes Prof. Dr. Francisco Tadeu Rantin Prof. Dr. Ana Lúcia Kalinin

Comparative Biochemistry

The research cover the intermediate metabolism, physiological stress and oxidative stress

Prof. Dr. Marisa Narciso Fernandes

Functional Morphology

Researches cover

Integrative laboratory studies of gills and accessory respiratory organs on fish presenting different mode of respiration with emphasis to the respiratory surface area and ion- and osmoregulation (ion transport and Na+/K+-ATPase)

Integrative environmental studies involving the environmental physiology and aquatic toxicology of aquatic animals including gills and liver, oxidative stress for the protection of aquatic and human health and of the environment.

Laboratory infra- structure

Aquarium

Aquarium room

Laboratory infra- structure

Multidisciplinary LaboratoryFor general biological materials processing

Biochemistry room

Microtome and ultramicrotome rooms

Microscopy room

Laboratory infra- structure

Electron microscopy centerDepartment of Material Sciences

Laboratory GroupDr. Cleoni S. Carvalho (UFSCar/ Campus Sorocaba)Dr. Marise M. Sakuragui (CNPq-INCT-TA)

Graduated StudentsMSc. Hugo Ribeiro (PhD Program in Physiological Sciences) - UNICEPMSc. Cleverson A. Ramos (PhD Program Ecology and Natural Resources) - CNPqMarcelo G. Paulino (MSc Program in Physiological Sciences) - CNPqNatália S. Shiogiri (MSc Program in Ecology and Natural Resources) - FAPESPIara C. Souza (MSc Program in Ecology and Natural Resources) - CNPqAndressa Reis (MSc Program in Ecology and Natural Resources) - Pref Vitoria (ES)

Undergraduate studentsTayrine P. Benze (UNIARA) – IC-CNPqPamella Gabriel (UNIARA)Graziella A. Andriani (UFSCar) – PET-CapesNaiara E.S. Souza (UFSCar) – IC-CNPQ/INCT-TAJakeline F. Ferreira (UFSCar) – BA-UFSCar

Technical assistance - Angelo Carmosi

Research Colaboration

Universidad Nacional de Córdoba (CONICET) Dr. Daniel A. WunderlinUniversidad de Cadiz Dr. Juan Miguel Mancera RomeroUniversidade do Porto Dr. Eduardo RochaUniversity of Bonn Dr. Steven S Perry

Universidade Estadual de Londrina, PR Dr. Claudia B R MartinezStudents: MSc. Juliana D Simonato (PhD Prog. Ecology and Natural Resources) - CNPq

MSc. Lindalva P. Manduenho (PhD Prog. Ecology and Natural Resources)- CNPq

Universidade de São Paulo/C. São Carlos, SP Dr. Evaldo L. G. EspíndolaStudent: MSc. Fabiano Tonissi (PhD Program in Environmental Engeneer) – CAPES

Universidade Estadual do Estado de São Paulo (UNESP/Jaboticabal) Dr. Claudinei Cruz

Universidade Federal do Amazonas, AM Dr. Oscar T F Costa/Dr. Wallice P Duncan

Inst Nacional de Pesquisas na Amazonia (INPA), AM Dr. Elizabeth Affonso

Universidade Federal de Tocantins Dr. Sandro E. Moron

Universidade Federal da Bahia Dr. Wilfried Klein

Centro Universitário de Vila Velha, ES Dr. Adriana R Chippari Gomes

Universidade Federal do Espírito Santo, ES Dr. Camila R. D. Milanez

Universidade Federal de Santa Maria, ES Dr. Bernardo Baldisserotto

Research in progress

Partitioning of diffusion capacity of fish with bimodal and multimodal mode of respiration

1. What are the physiological significance of anatomical phenotypes evolved to supplement gas exchange in aquatic animals?

2. Is the total diffusion capacity of respiratory organs of fish with bimodal and multimodal respiration similar in fish phylogenetic different but similar mode of life?

3. Is the maintenance of skin and/or gills as respiratory organs in air-breathing fish particularly related to CO2 excretion rather than O2 uptake?

Liposarcus pardalis

Electrophorus electricus

Hoplerythrinus unitaeniatusArapaima gigasLepidosiren paradoxa

M.N. Fernandes, S.F. Perry and collaborators

gills

lung

Research in progress

Amazonian freshwater stingrays: habitat, osmorregulation and gill morphology

Potamotrygon sp (cururu)

P. orbignyiP. leopoldi

P. schoroederi

Paratrygon aiereba

Potamotrygon motoro

Evaluate the phenotypic plasticity in potamotrigonids related to osmoregulatory variables, gill morphology and habitat in different water types of Amazon basin: Rio Negro (black water), Rio Solimões/Amazonas (white water) and Rio Branco (clear water)

M.N. Fernandes, W.P. Duncan and collaborators

Potamotrygon sp(cururu ray)

Research in progress

Aquatic Toxicology (INCT-TA/CNPq)Toxicity and changes in the gill morphology and function of curimbatá, Prochilodus lineatus exposed to sublethal concentrations of atrazine

Fish exposure: 48h and 14 days2 µgL-1 (CONAMA 357/2005 resolution)

10 µgL-1 ( ½ concentration applied in agriculture)25 µgL-1 (concentration applied in agriculture, Vitale et al., 1999)

AnalysesMorphology: Histopathology, chloride cell density (imunohistochemistry) and fractional area

(scanning electron microscopy)

Enzyme activity: oxidative stress (SOD, CAT, GPx, GR, GST), GSH, lipid peroxidation (LPO)Na+/K+-ATPase, H+-ATPase, carbonic anhydrase

Plasma osmolality and ions

Research in progress

Toxicity and changes in the gill morphology and function of curimbatá, Prochilodus lineatus exposed to sublethal concentrations of phenanthrene

Aquatic Toxicology (INCT-TA/CNPq)

CL50 determination

Fish exposure: sublethal concentrations: 48h and 14 days

AnalysesMorphology: Histopathology, chloride cell density (imunohistochemistry) and fractional area

(scanning electron microscopy)

Enzyme activity: oxidative stress (SOD, CAT, GPx, GR, GST), GSH, lipid peroxidation (LPO)Na+/K+-ATPase, H+-ATPase, carbonic anhydrase

Plasma osmolality and ions

Research in progress

Aquatic Toxicology (INCT-TA/CNPq)

Morphological and functional changes in pacu (Piaractus mesopotamicus) exposed to ROUNDUP® READY herbicide

Sensibility test

Acute toxicity test: 48h-CL50(I)

Chronic toxicity test: three concentrations CL50/5, CL50/10, CL50/50 and CL50/10014 days (samples at 1, 3, 5, 7, 11, 14 day)

AnalysesMorphology: gill and liver histopathology, chloride cell densityEnzyme activity: oxidative stress (SOD, CAT, GPx, GR, GST), GSH, lipid peroxide (LPO)

Na+/K+-ATPase

Plasma osmolality and ions

M.N. Fernandes; Claudinei da Cruz and collaborators

Environmental Field StudiesRio Monjolinho (São Carlos, SP) environmental evaluation

M.N. Fernandes, C. S. Carvalho, E. L. G. Espíndola and collaborators

Environmental Field StudiesWater monitoring and evaluation of health status of fish from Furnas Hydroelectric Power Station reservoir, MG

Furnas Hydroelectric Power Station reservoir

Flooded area : 1,440 km2

Water volume : 22 millions m3

Perimeter: 3,500 km

Financial support

Furnas Hydroelectric Power Station reservoir

Astyanax fasciatus Pimelodus maculatus

Water – organochlorine contamination in sites with high agriculture activity

Fish from contaminated sites

- no evidence of oxidative stress

• - Gills showing low to moderated lesions, chloride cell proliferation, increased Na+/K+-ATPase activity suggest a reduction on the respiratory function

- Liver showing severe lesions indicates a reduction of normal function

Environmental Field StudiesMonitoring and water quality control of Vitória Bay, Vitória, ES

main goal: to control the pollution level and all aspect of environmental degradation

Financial support

SecretariaMeio ambiente

Research includes: water, sediment, plankton, plants and animals (mollusks and fish)

Coordenation: Dr. Camila R. D. Milanez

Centropomus parallelus

Perna perna

Short coursesSummer Course in Ecology and Natural Resources

Biomarkers and Toxicology MSc. Juliana D Simonato and MSc. Lindalva P. Manduenho

Aquatic Toxicology Course during XVII Scientific Meeting of UFSCarDr. Marise M. Sakuragui, Marcelo G. Paulino, Natalia S. Shiogiri, Iara C. Souza

Scientific meetingsStructural and Functional characterization of hemoglobins of Lepidosiren paradoxa.

Effects of salinity on the osmoregulation of freshwater stingray Potamotrygon motoro (Chondrichthyes:Potamotrygonidae).

Rodlet cells in the gills of obligatory air-breather Arapaima gigas during its transition from aquatic to air-breather behavior.

Hepatic histopathology in two teleost species, Astyanax fasciatus and Pimelodus maculatus from Furnas Powerplant reservoir.

Na+/K+-ATPase activity and chloride cell density in the fish gills as biomarkers of water quality.

Na+/K+-ATPase activity changes in Oreochromis niloticus gills after cadmium, copper and zinc exposure.

White blood cells changes in Oreochromis niloticus exposed to cadmium, copper and zinc mean concentrations of the Monjolinho river, São Carlos, SP.

Acute toxicity of glyphosate in pacu (Piaractus mesopotamicus).

Histopatological changes in the liver of pacu (Piaractus mesopotamicus) exposed to glyphosate.

Histopatological changes in the gills of pacu (Piaractus mesopotamicus) exposed to glyphosate Roundup Ready.

The research of LBZC is reflected by the awards of

Fundação de Amparo à Pesquisa do Estado de São Paulo

CNPqCNPq Conselho Nacional de Desenvolvimento Científico e Tecnológico

C A P E SC A P E S

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

Financiadora de Estudos e Projetos

FURNAS Centrais Elétricas S.A.

Companhia Energética de Minas Gerais

WOTRO Sciences for Global Development

Prefeitura de Vitória, ES