dr tazeem hussain. mbbs,mcps,fcps ed.ksmc. . common haematology values haemoglobin men: 13–18g/dl...
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LAB. INTERPRETATION CBC,ELECTROLYTES,LFT
VBG /ABG
DR TAZEEM HUSSAIN . MBBS,MCPS,FCPS
ED.KSMC
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Common haematology valuesHaemoglobin men: 13–18g/dL women: 11.5–16g/dL Mean cell volume, mcv76–96fL ↓; ↑Platelets 150–400 × 109/L White cells (total) 4–11 × 109/L Neutrophils 40–75% lymphocytes 20–45% Eosinophils 1–6%If outside this range, consult:
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. Microcytic Hypochromic Anemia (MCV < 83; MCHC < 31)
Condition Serum IronTotal Iron-Binding
Capacity (TIBC)Bone Marrow Iron Comment
Iron deficiency ↓ ↑ 0 Responsive to iron therapy
Chronic inflammation
↓ ↓ ++ Unresponsive to iron therapy
Thalassemia major ↑ N ++++ Reticulocytosis and indirect bilirubinemia
Thalassemia minor N N - ↓ ++ Elevation of fetal hemoglobin and Hb A2, target cells, and poikilocytosis
Lead poisoning N N ++ Basophilic stippling of RBCs
Sideroblastic ↑ N ++++ Ring sideroblasts in marrow
Hemoglobin N N ++ Hemoglobin electrophoresis
↓ = decreased; ↑ = increased; 0 = absent; +'s indicate the amount of stainable iron in bone marrow specimens, on a scale of 0-4; N = normal.
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Macrocytic Anemia (MCV >95)Megaloblastic bone marrow Deficiency of vitamin B-12
Deficiency of folic acid
Drugs affecting deoxyribonucleic acid (DNA) synthesis
Inherited disorders of DNA synthesis
Nonmegaloblastic bone marrow Liver disease
Hypothyroidism and hypopituitarism
Accelerated erythropoiesis (reticulocytes)
Hypoplastic and aplastic anemia
Infiltrated bone marrow
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Macrocyte Larger than normal (>8.5 µm diameter).
Microcyte Smaller than normal (< 7 µm diameter).
Hypochromic Less hemoglobin in cell. Enlarged area of central pallor.
Spherocyte Loss of central pallor, stains more densely, often microcytic. Hereditary spherocytosis and certain acquired hemolytic anemias
Target cell Hypochromic with central "target" of hemoglobin. Liver disease, thalassemia, hemoglobin D, and postsplenectomy
Leptocyte Hypochromic cell with a normal diameter and decreased MCV. Thalassemia
Elliptocyte Oval to cigar shaped. Hereditary elliptocytosis, certain anemias (particularly vitamin B-12 and folate deficiency)
Schistocyte Fragmented helmet- or triangular-shaped RBCs. Microangiopathic anemia, artificial heart valves, uremia, and malignant hypertension
Stomatocyte Slitlike area of central pallor in erythrocyte. Liver disease, acute alcoholism, malignancies, hereditary stomatocytosis, and artifact
Various Forms of RBCs
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.Tear-shaped RBCs Drop-shaped erythrocyte, often microcytic. Myelofibrosis and infiltration of marrow with tumor. Thalassemia
Acanthocyte Five to 10 spicules of various lengths and at irregular intervals on surface of RBCs
Echinocyte Evenly distributed spicules on surface of RBCs, usually 10-30. Uremia, peptic ulcer, gastric carcinoma, pyruvic kinase deficiency, and preparative artifact
Sickle cell Elongated cell with pointed ends. Hemoglobin S and certain types of hemoglobin C and l
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Classification of the Hemolytic Disorders
Hereditary Acquired
Intracorpuscular defect
Hereditary spherocytosis Hereditary elliptocytosis Hemoglobinopathies Thalassemias Congenital dyserythropoietic anemias Hereditary RBC enzymatic deficiencies Rarer hereditary abnormalities
Vitamin B-12 and folic acid deficiency Paroxysmal nocturnal hemoglobinuria Severe iron deficiency
Extracorpuscular defect
Physical agents: Burns, cold exposure Traumatic: Prosthetic heart valves, march hemoglobinuria, disseminated intravascular coagulation (DIC), graft rejection Chemicals: Drugs and venoms
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College of Physicians & Surgeons Pakistan
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urea and electrolytesSodium 135–145 mmol/L Potassium 3.5–5 mmol/L Creatinine 70–150 μ mol/L Urea 2.5–6.7 mmol/L If outside this range, consult:
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LFTBilirubin 3–17 μmol/L Alanine aminotransferase, alt 5–35iu/L
Aspartate transaminase, ast 5–35iu/L
Alkaline phosphatase, alp 30–150iu/L
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.Blood gasespH 7.35–7.45 P aO2 >10.6kPa (75–100mmHg) P aCO2 4.7–6kPa (35–45mmHg) Base excess ±2mmol/L
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Acid–base balanceArterial blood pH is closely regulated in
health to 7.40 ± 0.05 by various mechanisms including bicarbonate, other plasma buffers such as deoxygenated hemoglobin, and the kidney.
a few simple rules are applied, then interpretation and diagnosis are easy. The key principle is that primary changes in
H CO–3 metabolic CO2 respiratory
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A simple method1 Look at the pH: is there an acidosis or alkalosis?CO2 is an acidic gas—is CO2 raised with an
acidosis, lowered with an alkalosis? If so, it is in keeping with the
pH and thus caused by a respiratoryproblem. If there is no change, or an
opposite one, then the change is compensatory.
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.3 Is the HCO 3 abnormal? (Normal
concentration 22–28mmol/L)If so, is the change in keeping with the pH? is alkaline—HCO3 is raised with an alkalosis, lowered with an acidosis? If so, the problem is metabolic
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.Your patient’s blood gas shows: pH 7.05, CO2
2.0kPa, HCO 3 8.0mmol/L.There is an acidosis. The CO2 is low, and thus
it is a compensatory change. TheHCO3is low and is thus the primary change, ie a metabolic acidosis.
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.The anion gapestimates unmeasured plasma anions (‘fixed’
or organic acids such as phosphate, ketones, and lactate, which are hard to
measure directly). It is calculated as the difference between plasma cations (Na & K ) and anions (Cl &HCO3 ).
Normal range: 10–18mmol/L. It is helpful in determining the cause of a metabolic acidosis.
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.Metabolic acidosispH↓, HCO3↓Causes of metabolic acidosis and an increased anion gap:Due to increased production, or reduced excretion, of
fixed/organic acids. HCO 3 falls and unmeasured anionsassociated with the acids accumulate.• Lactic acid (shock, infection, tissue ischaemia)• Urate (renal failure)• Ketones (diabetes mellitus, alcohol)• Drugs/toxins (salicylates, biguanides, ethylene glycol,
methanol)
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Causes of metabolic acidosis and a normal anion gap:
Due to loss of bicarbonate or ingestion of H ions (Cl is retained).
• Renal tubular acidosis• Diarrhoea• Drugs (acetazolamide)• Addison’s disease• Pancreatic fistula• Ammonium chloride ingestion
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.Metabolic alkalosispH↑, HCO 3 ↑• Vomiting• K depletion (diuretics)• Burns• Ingestion of base
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Respiratory acidosis
pH↓, CO ↑• Type 2 respiratory failure due to any lung,
neuromuscular, or physical cause • Most commonly chronic obstructive
pulmonary disease (COPD). Look at the P O2 . It will probably be low.
Is oxygentherapy required? Use controlled O2 (Venturi connector) if COPD is the underlying cause, as too much oxygen may make matters worse
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.Respiratory alkalosispH↑, CO ↓A result of hyperventilation of any cause.CNS causes:Stroke; subarachnoid bleed; meningitis.
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SEE.in VBG.Step 1. PH.Step 2. BICARBONATE.Step 3. CARBON DI OXIDE.Step 4. compensation ?
IF ABG.See oxygen /carbon di oxide.
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THANKS -----------?????