e coli testing
TRANSCRIPT
Coliquant EZ Gel
What it is
How to do it
What it tells you
What it is
Petri Dish culture testFor E coli and Coliform Bacteria
Indicator Species
Feces come from animalsFeces can’t be detected (spread out)Certain bacteria are found
exclusively with feces= indicator bacteria
Bacteria
How do bacteria reproduce?AsexuallyClusters of bacteria therefore form,
known as coloniesColonies can be counted (bacteria
too small alone)
Field Sampling
A small bottle of water is filledExtra care is taken to:
Keep the sample bottle cleanKeep the sample bottle cold
The small bottle then gets poured onto petri dish back at lab
Incubation
A measured amount of water sample is then poured onto a medium
The medium feeds the bacteria.This is called “culturing” bacteriaWith food and warmth they multiply
and form colonies
Analysis
Colonies/100 mLColor of colony indicates if it’s a
strain of coliform and e coli
Colors
Deep purple= E coliTeal= not E coli
Pink= not E coli