ecography ecog-02908 · 2017. 2. 2. · x084 scytodidae stedocys sp. 17 china hechi, guangxi n/a...

Ecography ECOG-02908Luo, Y. and Li, S. 2017. Cave Stedocys spitting spiders illuminate the history of the Himalayas and southeast Asia. – Ecography doi: 10.1111/ecog.02908

Supplementary material

Appendix 1

Fig. A4. (A) Phylogenetic tree reconstructed using maximum likelihood inference based on the concatenated mitochondria and nuclear gene data. Branch is truncated as indicated by slashes. Blue numbers at nodes indicate maximum likelihood bootstrap support values, and “-” at nodes indicate that the bootstrap values are lower than 50%. Black numbers near branches are in-group divergence time nodes. Branch lengths indicate the expected number of substitutions per site. (B) Divergence times based on fossil calibration under uncorrelated exponential relaxed clock. Nodes are available in Figure 1SA. 95% CI* is 95% highest posterior density. Ma is million years ago.

Table A5. Samples used in this study: specimen label, haplotype, taxon name, sample collection locality with coordinates, and GenBank

accession numbers.

Specimen code Family Genus Species Country Locality Lat/long Altitude Haplotype CO1 28S ITS2 16S H3 18S X001 Scytodidae Stedocys sp. 1 China Guilin, Guangxi 25.309/110.264 164 hap1 submitting submitting submitting submitting submitting submitting X002 Scytodidae Stedocys sp. 1 China Guilin, Guangxi 25.309/110.264 164 hap4 submitting submitting submitting submitting submitting submitting X003 Scytodidae Stedocys sp. 1 China Guilin, Guangxi 25.309/110.264 164 hap7 submitting submitting submitting submitting submitting submitting X004 Scytodidae Stedocys sp. 1 China Guilin, Guangxi 25.309/110.264 164 hap4 submitting submitting submitting submitting submitting submitting X005 Scytodidae Stedocys sp. 1 China Guilin, Guangxi 25.309/110.264 164 hap2 submitting submitting submitting submitting submitting submitting X006 Scytodidae Stedocys sp. 1 China Guilin, Guangxi 25.309/110.264 164 hap8 submitting submitting submitting submitting submitting submitting X007 Scytodidae Stedocys sp. 1 China Guilin, Guangxi 25.309/110.264 164 hap8 submitting submitting submitting submitting submitting submitting X008 Scytodidae Stedocys sp. 1 China Guilin, Guangxi 25.309/110.264 164 hap4 submitting submitting submitting submitting submitting submitting X010 Scytodidae Stedocys sp. 1 China Guilin, Guangxi 25.309/110.264 164 hap5 submitting submitting submitting submitting submitting submitting X011 Scytodidae Stedocys sp. 1 China Guilin, Guangxi 25.309/110.264 164 hap2 submitting submitting submitting submitting submitting submitting X012 Scytodidae Stedocys sp. 1 China Guilin, Guangxi 25.309/110.264 164 hap2 submitting submitting submitting submitting submitting submitting X013 Scytodidae Stedocys sp. 1 China Guilin, Guangxi 25.309/110.264 164 hap3 submitting submitting submitting submitting submitting submitting X014 Scytodidae Stedocys sp. 1 China Guilin, Guangxi 25.513/110.243 175 hap9 submitting submitting submitting submitting submitting submitting X015 Scytodidae Stedocys sp. 1 China Guilin, Guangxi 25.513/110.243 175 hap9 submitting submitting submitting submitting submitting submitting X016 Scytodidae Stedocys sp. 1 China Guilin, Guangxi 25.513/110.243 175 hap9 submitting submitting submitting submitting submitting submitting X017 Scytodidae Stedocys sp. 1 China Guilin, Guangxi 25.513/110.243 175 hap9 submitting submitting submitting submitting submitting submitting X018 Scytodidae Stedocys sp. 2 China Liuzhou, Guangxi 24.297/109.405 85 hap13 submitting submitting submitting submitting submitting submitting X019 Scytodidae Stedocys sp. 2 China Liuzhou, Guangxi 24.297/109.405 85 hap13 submitting submitting submitting submitting submitting submitting X020 Scytodidae Stedocys sp. 2 China Liuzhou, Guangxi 24.297/109.405 85 hap14 submitting submitting submitting submitting submitting submitting X021 Scytodidae Stedocys sp. 2 China Liuzhou, Guangxi 24.297/109.405 85 hap13 submitting submitting submitting submitting submitting submitting X022 Scytodidae Stedocys sp. 2 China Liuzhou, Guangxi 24.297/109.405 85 hap14 submitting submitting submitting submitting submitting submitting X023 Scytodidae Stedocys sp. 2 China Liuzhou, Guangxi 24.297/109.405 85 hap14 submitting submitting submitting submitting submitting submitting X027 Scytodidae Stedocys sp. 2 China Liuzhou, Guangxi 24.297/109.405 85 hap14 submitting submitting submitting submitting submitting submitting X028 Scytodidae Stedocys sp. 2 China Liuzhou, Guangxi 24.297/109.405 85 hap13 submitting submitting submitting submitting submitting submitting X029 Scytodidae Stedocys sp. 2 China Liuzhou, Guangxi 24.297/109.405 85 hap13 submitting submitting submitting submitting submitting submitting X030 Scytodidae Stedocys sp. 2 China Liuzhou, Guangxi 24.227/109.410 130 hap16 submitting submitting submitting submitting submitting submitting X031 Scytodidae Stedocys sp. 2 China Liuzhou, Guangxi 24.227/109.410 130 hap16 submitting submitting submitting submitting submitting submitting X032 Scytodidae Stedocys sp. 2 China Liuzhou, Guangxi 24.227/109.410 130 hap16 submitting submitting submitting submitting submitting submitting X033 Scytodidae Stedocys sp. 2 China Liuzhou, Guangxi 24.227/109.410 130 hap15 submitting submitting submitting submitting submitting submitting X034 Scytodidae Stedocys sp. 2 China Yizhou, Guangxi 24.489/108.570 205 hap12 submitting submitting submitting submitting submitting submitting X035 Scytodidae Stedocys sp. 2 China Laibin, Guangxi 23.727/109.091 89 hap12 submitting submitting submitting submitting submitting submitting X036 Scytodidae Stedocys sp. 2 China Laibin, Guangxi 23.727/109.091 89 hap12 submitting submitting submitting submitting submitting submitting

X037 Scytodidae Stedocys sp. 2 China Laibin, Guangxi 23.727/109.091 89 hap11 submitting submitting submitting submitting submitting submitting X038 Scytodidae Stedocys sp. 2 China Laibin, Guangxi 23.727/109.091 89 hap12 submitting submitting submitting submitting submitting submitting X039 Scytodidae Stedocys sp. 3 China Laibin, Guangxi 23.955/109.662 114 hap20 submitting submitting submitting submitting submitting submitting X040 Scytodidae Stedocys sp. 3 China Laibin, Guangxi 23.727/109.091 89 hap19 submitting submitting submitting submitting submitting submitting X041 Scytodidae Stedocys sp. 3 China Laibin, Guangxi 23.955/109.662 114 hap18 submitting submitting submitting submitting submitting submitting X042 Scytodidae Stedocys sp. 4 China Guilin, Guangxi 24.762/105.867 153 hap10 submitting submitting submitting submitting submitting submitting

X043 Scytodidae Stedocys sp. 5 China Qianxinan, Guizhou 25.433/105.612 1056 hap21 submitting submitting submitting submitting submitting submitting




X047 Scytodidae Stedocys sp. 6 China Wenshan, Yunnan 23.507/104.902 1301 hap23 submitting submitting submitting submitting submitting submitting X048 Scytodidae Stedocys sp. 7 Vietnam Na phong, Bac Kan 22.464/105.650 169 hap25 submitting submitting submitting submitting submitting submitting X049 Scytodidae Stedocys sp. 7 Vietnam 22.464/105.650 169 hap25 submitting submitting submitting submitting submitting submitting

X050 Scytodidae Stedocys sp. 7 Vietnam Tham Thiuh, Bac

Kan 22.401/105.632 146 hap26 submitting submitting submitting submitting submitting submitting

X051 Scytodidae Stedocys sp. 7 Vietnam Tham Thiuh, Bac

Kan 22.350/105.687 193 hap24 submitting submitting submitting submitting submitting submitting

X052 Scytodidae Stedocys sp. 7 Vietnam Ba Be National Park, Bac Kan 22.380/105.613 225 hap29 submitting submitting submitting submitting submitting submitting

X053 Scytodidae Stedocys sp. 7 Vietnam Na phong, Bac Kan 22.390/105.614 179 hap29 submitting submitting submitting submitting submitting submitting X054 Scytodidae Stedocys sp. 7 Vietnam Pac Chan, Bac Kan 22.380/105.613 225 hap29 submitting submitting submitting submitting submitting submitting




X058 Scytodidae Stedocys sp. 7 Vietnam Na phong, Bac Kan 22.390/105.614 179 hap27 submitting submitting submitting submitting submitting submitting X059 Scytodidae Stedocys sp. 10 China Hechi, Guangxi 22.715/107.837 99 hap30 submitting submitting submitting submitting submitting submitting X061 Scytodidae Stedocys sp. 10 China Hechi, Guangxi 22.715/107.837 99 hap30 submitting submitting submitting submitting submitting submitting X062 Scytodidae Stedocys sp. 8 China Daxin, Guangxi 22.965/107.470 256 hap39 submitting submitting submitting submitting submitting submitting

X063 Scytodidae Stedocys sp. 8 China Chong zuo,

Guangxi 22.965/107.470 256 hap39 submitting submitting submitting submitting submitting submitting




Guangxi 22.965/107.470 256 hap39 submitting submitting submitting submitting submitting submitting X066 Scytodidae Stedocys sp. 8 China Chong zuo, 22.965/107.470 256 hap39 submitting submitting submitting submitting submitting submitting

Guangxi X067 Scytodidae Stedocys sp. 8 China N/A N/A N/A hap40 submitting submitting submitting submitting submitting submitting






Guangxi 22.963/107.066 352 hap38 submitting submitting submitting submitting submitting submitting X071 Scytodidae Stedocys sp. 9 China Nanning, Guangxi 22.959/107.616 241 hap34 submitting submitting submitting submitting submitting submitting X072 Scytodidae Stedocys sp. 9 China Nanning, Guangxi 22.959/107.616 241 hap34 submitting submitting submitting submitting submitting submitting X073 Scytodidae Stedocys sp. 9 China Nanning, Guangxi 22.959/107.616 241 hap34 submitting submitting submitting submitting submitting submitting X074 Scytodidae Stedocys sp. 9 China Nanning, Guangxi 22.959/107.616 241 hap34 submitting submitting submitting submitting submitting submitting X075 Scytodidae Stedocys sp. 9 China Nanning, Guangxi 22.959/107.616 241 hap33 submitting submitting submitting submitting submitting submitting X076 Scytodidae Stedocys sp. 9 China Nanning, Guangxi 22.959/107.616 241 hap31 submitting submitting submitting submitting submitting submitting

X077 Scytodidae Stedocys sp. 16 Vietnam Dam Tao National

Park, Phu Quoc 21.472/105.641 1023 hap41 submitting submitting submitting submitting submitting submitting X078 Scytodidae Stedocys sp. 16 Vietnam Puong, Bac Kan 21.472/105.641 1023 hap42 submitting submitting submitting submitting submitting submitting X079 Scytodidae Stedocys sp. 17 China Hechi, Guangxi 24.705/107.936 215 hap51 submitting submitting submitting submitting submitting submitting X081 Scytodidae Stedocys sp. 17 China Hechi, Guangxi 24.705/107.936 215 hap51 submitting submitting submitting submitting submitting submitting X082 Scytodidae Stedocys sp. 17 China Hechi, Guangxi 24.687/107.877 268 hap50 submitting submitting submitting submitting submitting submitting X083 Scytodidae Stedocys sp. 17 China Hechi, Guangxi 24.076/107.671 550 hap54 submitting submitting submitting submitting submitting submitting X084 Scytodidae Stedocys sp. 17 China Hechi, Guangxi N/A N/A hap56 submitting submitting submitting submitting submitting submitting X085 Scytodidae Stedocys sp. 17 China Hechi, Guangxi 24.076/107.671 550 hap54 submitting submitting submitting submitting submitting submitting X086 Scytodidae Stedocys sp. 19 China Baise, Guangxi N/A N/A hap43 submitting submitting submitting submitting submitting submitting X087 Scytodidae Stedocys sp. 19 China Baise, Guangxi N/A N/A hap43 submitting submitting submitting submitting submitting submitting X088 Scytodidae Stedocys sp. 19 China Baise, Guangxi 23.567/107.680 285 Hap43 submitting submitting submitting submitting submitting submitting X089 Scytodidae Stedocys sp. 19 China Baise, Guangxi N/A N/A hap43 submitting submitting submitting submitting submitting submitting X090 Scytodidae Stedocys sp. 19 China Baise, Guangxi 23.567/107.680 285 hap43 submitting submitting submitting submitting submitting submitting X091 Scytodidae Stedocys sp. 19 China Baise, Guangxi 23.567/107.680 285 hap43 submitting submitting submitting submitting submitting submitting X092 Scytodidae Stedocys sp. 19 China Baise, Guangxi 23.567/107.680 285 hap43 submitting submitting submitting submitting submitting submitting X093 Scytodidae Stedocys sp. 19 China Baise, Guangxi 23.567/107.680 285 hap43 submitting submitting submitting submitting submitting submitting X094 Scytodidae Stedocys sp. 18 China Dahua, Guangxi 23.693/107.822 188 hap46 submitting submitting submitting submitting submitting submitting X095 Scytodidae Stedocys sp. 18 China Dahua, Guangxi 23.693/107.822 188 hap45 submitting submitting submitting submitting submitting submitting X096 Scytodidae Stedocys sp. 18 China Dahua, Guangxi 23.693/107.822 188 hap44 submitting submitting submitting submitting submitting submitting X097 Scytodidae Stedocys sp. 18 China Dahua, Guangxi 23.693/107.822 188 hap46 submitting submitting submitting submitting submitting submitting X099 Scytodidae Stedocys sp. 18 China Baise, Guangxi 23.806/107.525 293 hap47 submitting submitting submitting submitting submitting submitting X100 Scytodidae Stedocys sp. 11 China Baise, Guangxi 24.951/106.380 773 hap77 submitting submitting submitting submitting submitting submitting X101 Scytodidae Stedocys sp. 11 China Baise, Guangxi 24.951/106.380 773 hap77 submitting submitting submitting submitting submitting submitting X102 Scytodidae Stedocys sp. 11 China Baise, Guangxi 24.951/106.380 773 hap78 submitting submitting submitting submitting submitting submitting

X103 Scytodidae Stedocys sp. 11 China Baise, Guangxi 24.774/106.542 914 hap79 submitting submitting submitting submitting submitting submitting X104 Scytodidae Stedocys sp. 11 China Baise, Guangxi 24.774/106.542 914 hap79 submitting submitting submitting submitting submitting submitting X105 Scytodidae Stedocys sp. 11 China Lingyun, Guangxi 24.367/106.578 445 hap66 submitting submitting submitting submitting submitting submitting X106 Scytodidae Stedocys sp. 11 China Hechi, Guangxi 24.566/107.041 574 hap75 submitting submitting submitting submitting submitting submitting X107 Scytodidae Stedocys sp. 11 China Lingyun, Guangxi 24.346/106.558 470 hap64 submitting submitting submitting submitting submitting submitting X108 Scytodidae Stedocys sp. 11 China Bama, Guangxi 24.302/107.116 438 hap71 submitting submitting submitting submitting submitting submitting X110 Scytodidae Stedocys sp. 14 China Baise, Guangxi N/A N/A hap87 submitting submitting submitting submitting submitting submitting X111 Scytodidae Stedocys sp. 14 China Baise, Guangxi N/A N/A hap88 submitting submitting submitting submitting submitting submitting X112 Scytodidae Stedocys sp. 14 China Baise, Guangxi 24.870/105.195 941 hap86 submitting submitting submitting submitting submitting submitting X113 Scytodidae Stedocys sp. 14 China Baise, Guangxi 24.870/105.195 724 hap86 submitting submitting submitting submitting submitting submitting X114 Scytodidae Stedocys sp. 14 China Baise, Guangxi 24.742/105.380 724 hap89 submitting submitting submitting submitting submitting submitting X115 Scytodidae Stedocys sp. 14 China Baise, Guangxi 24.742/105.380 724 hap89 submitting submitting submitting submitting submitting submitting X116 Scytodidae Stedocys sp. 14 China Baise, Guangxi 24.742/105.380 724 hap89 submitting submitting submitting submitting submitting submitting X117 Scytodidae Stedocys sp. 12 China Qiannan, Guizhou N/A N/A hap63 submitting submitting submitting submitting submitting submitting X118 Scytodidae Stedocys sp. 15 China Honghe, Yunnan 23.368/103.535 1735 hap92 submitting submitting submitting submitting submitting submitting

X119 Scytodidae Stedocys sp. 21 Laos Khamkeut District,

Bolikhamxay 18.222/104.813 495 hap97 submitting submitting submitting submitting submitting submitting X120 Scytodidae Stedocys sp. 22 China Baoheng, Hainan 18.598/109.427 616 hap106 submitting submitting submitting submitting submitting submitting X121 Scytodidae Stedocys sp. 22 China Baoheng, Hainan 18.598/109.427 616 hap106 submitting submitting submitting submitting submitting submitting X122 Scytodidae Stedocys sp. 22 China Baoheng, Hainan 18.598/109.427 616 hap105 submitting submitting submitting submitting submitting submitting X123 Scytodidae Stedocys sp. 22 China Changjiang, Hainan 19.119/109.152 684 hap104 submitting submitting submitting submitting submitting submitting X124 Scytodidae Stedocys sp. 22 China Wangning, Hainan 18.965/110.422 290 hap100 submitting submitting submitting submitting submitting submitting X125 Scytodidae Stedocys sp. 22 China Dongfang, Hainan 19.100/109.024 291 hap98 submitting submitting submitting submitting submitting submitting X240 Scytodidae Stedocys sp. 11 China Hechi, Guangxi 22.715/107.837 480 hap73 submitting submitting submitting submitting submitting submitting X241 Scytodidae Stedocys sp. 14 China Baise, Guangxi 24.742/105.380 724 hap90 submitting submitting submitting submitting submitting submitting X245 Scytodidae Stedocys sp. 3 China Liuzhou, Guangxi 24.752/109.660 285 hap17 submitting submitting submitting submitting submitting submitting X246 Scytodidae Stedocys sp. 6 China Wenshan, Yunnan 23.545/105.728 1096 hap22 submitting submitting submitting submitting submitting submitting X251 Scytodidae Stedocys sp. 1 China Guilin, Guangxi 25.309/110.264 164 hap6 submitting submitting submitting submitting submitting submitting X252 Scytodidae Stedocys sp. 14 China Baise, Guangxi N/A N/A hap87 submitting submitting submitting submitting submitting submitting X254 Scytodidae Stedocys sp. 11 China Baise, Guangxi 24.774/106.542 914 hap80 submitting submitting submitting submitting submitting submitting X259 Scytodidae Stedocys sp. 1 China Guilin, Guangxi 25.513/110.243 175 hap9 submitting submitting submitting submitting submitting submitting X260 Scytodidae Stedocys sp. 9 China Nanning, Guangxi 22.959/107.616 241 hap32 submitting submitting submitting submitting submitting submitting X263 Scytodidae Stedocys sp. 1 China Guilin, Guangxi 25.309/110.264 164 hap4 submitting submitting submitting submitting submitting submitting X264 Scytodidae Stedocys sp. 1 China Guilin, Guangxi 25.309/110.264 164 hap4 submitting submitting submitting submitting submitting submitting

X265 Scytodidae Stedocys sp. 21 Laos Khamkeut District,

Bolikhamxay 18.222/104.813 495 hap97 submitting submitting submitting submitting submitting submitting X267 Scytodidae Stedocys sp. 5 China Qiannan, Guizhou 25.433/105.612 1056 hap21 submitting submitting submitting submitting submitting submitting X268 Scytodidae Stedocys sp. 5 China Qiannan, Guizhou 25.433/105.612 1056 hap21 submitting submitting submitting submitting submitting submitting X269 Scytodidae Stedocys sp. 17 China Hechi, Guangxi 24.705/107.936 215 hap51 submitting submitting submitting submitting submitting submitting

X271 Scytodidae Stedocys sp. 18 China Dahua, Guangxi 23.693/107.822 188 hap44 submitting submitting submitting submitting submitting submitting X272 Scytodidae Stedocys sp. 12 China Qiannan, Guizhou 25.855/107.161 958 hap63 submitting submitting submitting submitting submitting submitting X275 Scytodidae Stedocys sp. 18 China Dahua, Guizhou 23.693/107.822 188 hap44 submitting submitting submitting submitting submitting submitting X277 Scytodidae Stedocys sp. 1 China Guilin, Guangxi 25.513/110.243 175 hap9 submitting submitting submitting submitting submitting submitting X278 Scytodidae Stedocys sp. 19 China Baise, Guangxi N/A N/A hap43 submitting submitting submitting submitting submitting submitting X295 Scytodidae Stedocys sp. 22 China Wangning, Hainan 18.966/110.417 191 hap102 submitting submitting submitting submitting submitting submitting

X297 Scytodidae Stedocys sp. 23 Thailand Sal Yok District,

Kanchanaburi 14.203/99.028 185 hap122 submitting submitting submitting submitting submitting submitting



X304 Scytodidae Stedocys sp. 26 Thailand Kaeng Koi District,

Saraburi 14.660/100.969 73 hap124 submitting submitting submitting submitting submitting submitting

X309 Scytodidae Stedocys sp. 23 Thailand Kraburi District,

Ranong 10.326/98.765 6 hap115 submitting submitting submitting submitting submitting submitting

X312 Scytodidae Stedocys sp. 24 Thailand Tap Pud District,

Phang Nga 8.5190/98.565 116 hap111 submitting submitting submitting submitting submitting submitting






Kanchanaburi 14.203/99.024 342 hap120 submitting submitting submitting submitting submitting submitting X326 Scytodidae Stedocys sp. 22 China Changjiang, Hainan 19.101/109.020 125 hap99 submitting submitting submitting submitting submitting submitting X328 Scytodidae Stedocys sp. 22 China Changli, Hainan 19.084/109.121 530 hap103 submitting submitting submitting submitting submitting submitting

X329 Scytodidae Stedocys sp. 24 Thailand Muang District,

Krabi 8.013/98.842 27 hap112 submitting submitting submitting submitting submitting submitting





X336 Scytodidae Stedocys sp. 24 Thailand Muang District,

Krabi 8.136/98.807 523 hap107 submitting submitting submitting submitting submitting submitting X337 Scytodidae Stedocys sp. 22 China Wangning, Hainan 18.967/110.418 210 hap101 submitting submitting submitting submitting submitting submitting

X367 Scytodidae Stedocys sp. 20 Vietnam

Cuc Phuong National Park, Ninh

Binh 20.356/105.605 115 hap95 submitting submitting submitting submitting submitting submitting X375 Scytodidae Stedocys sp. 11 China Baise, Guangxi 24.367/106.578 113 hap81 submitting submitting submitting submitting submitting submitting X376 Scytodidae Stedocys sp. 11 China Baise, Guangxi 24.574/106.225 1989 hap77 submitting submitting submitting submitting submitting submitting X387 Scytodidae Stedocys sp. 13 China Mashan, Guangxi 23.556/108.262 2060 hap85 submitting submitting submitting submitting submitting submitting X388 Scytodidae Stedocys sp. 18 China Hechi, Guangxi 23.919/107.625 1939 hap49 submitting submitting submitting submitting submitting submitting X389 Scytodidae Stedocys sp. 18 China Baise, Guangxi 23.806/107.525 340 hap48 submitting submitting submitting submitting submitting submitting X397 Scytodidae Stedocys sp. 9 China Nanning, Guangxi 23.210/107.591 15 hap36 submitting submitting submitting submitting submitting submitting

X398 Scytodidae Stedocys sp. 9 China Baise, Guangxi 23.209/107.584 95 hap35 submitting submitting submitting submitting submitting submitting X399 Scytodidae Stedocys sp. 21 China Yuxi, Yunnan 23.973/101.526 94 hap96 submitting submitting submitting submitting submitting submitting X400 Scytodidae Stedocys sp. 15 China Wenshan, Yunnan 23.399/103.125 33 hap94 submitting submitting submitting submitting submitting submitting X404 Scytodidae Stedocys sp. 15 China Wenshan, Yunnan 23.784/102.816 180 hap93 submitting submitting submitting submitting submitting submitting X405 Scytodidae Stedocys sp. 15 China Hechi, Guangxi 24.338/107.909 23 hap91 submitting submitting submitting submitting submitting submitting

X407 Scytodidae Stedocys sp. 24 Thailand Mueang District,

Phangnga 8.466/98.532 229 hap108 submitting submitting submitting submitting submitting submitting

X410 Scytodidae Stedocys sp. 25 Thailand Khuan Don

District, Satun 6.763/100.064 559 hap123 submitting submitting submitting submitting submitting submitting





X425 Scytodidae Stedocys sp. 23 Thailand Than To District,

Yala 6.208/101.166 400 hap114 submitting submitting submitting submitting submitting submitting

X426 Scytodidae Stedocys sp. 24 Thailand Ao Luk District,

Krabi 8.404/98.729 173 hap113 submitting submitting submitting submitting submitting submitting X434 Scytodidae Stedocys sp. 17 China Hechi, Guangxi 24.039/108.062 446 hap55 submitting submitting submitting submitting submitting submitting X435 Scytodidae Stedocys sp. 17 China Hechi, Guangxi 24.160/108.008 412 hap52 submitting submitting submitting submitting submitting submitting X436 Scytodidae Stedocys sp. 12 China Hechi, Guangxi 25.036/107.417 385 hap58 submitting submitting submitting submitting submitting submitting X437 Scytodidae Stedocys sp. 12 China Hechi, Guangxi 25.036/107.417 726 hap59 submitting submitting submitting submitting submitting submitting X438 Scytodidae Stedocys sp. 12 China Hechi, Guangxi 25.100/107.656 363 hap60 submitting submitting submitting submitting submitting submitting X439 Scytodidae Stedocys sp. 11 China Hechi, Guangxi 24.361/107.392 464 hap69 submitting submitting submitting submitting submitting submitting X440 Scytodidae Stedocys sp. 11 China Baise, Guangxi 24.367/106.578 618 hap67 submitting submitting submitting submitting submitting submitting X441 Scytodidae Stedocys sp. 17 China Hechi, Guangxi 24.073/108.054 449 hap53 submitting submitting submitting submitting submitting submitting X442 Scytodidae Stedocys sp. 12 China Hechi, Guangxi 25.394/108.256 402 hap61 submitting submitting submitting submitting submitting submitting X443 Scytodidae Stedocys sp. 11 China Hechi, Guangxi 24.528/107.104 244 hap74 submitting submitting submitting submitting submitting submitting X444 Scytodidae Stedocys sp. 11 China Hechi, Guangxi 24.448/107.347 635 hap68 submitting submitting submitting submitting submitting submitting X445 Scytodidae Stedocys sp. 11 China Baise, Guangxi 24.239/106.595 325 hap65 submitting submitting submitting submitting submitting submitting X446 Scytodidae Stedocys sp. 11 China Hechi, Guangxi 24.432/106.970 195 hap82 submitting submitting submitting submitting submitting submitting X447 Scytodidae Stedocys sp. 11 China Hechi, Guangxi 24.867/107.192 523 hap76 submitting submitting submitting submitting submitting submitting X448 Scytodidae Stedocys sp. 11 China Hechi, Guangxi 24.501/107.270 210 hap70 submitting submitting submitting submitting submitting submitting X449 Scytodidae Stedocys sp. 11 China Hechi, Guangxi 24.432/106.967 115 hap83 submitting submitting submitting submitting submitting submitting X450 Scytodidae Stedocys sp. 11 China Hechi, Guangxi 24.331/106.987 113 hap84 submitting submitting submitting submitting submitting submitting X451 Scytodidae Stedocys sp. 11 China Hechi, Guangxi 24.310/107.002 1989 hap72 submitting submitting submitting submitting submitting submitting X452 Scytodidae Stedocys sp. 12 China Hechi, Guangxi 24.770/108.390 2060 hap62 submitting submitting submitting submitting submitting submitting X453 Scytodidae Stedocys sp. 12 China Hechi, Guangxi 25.083/110.264 1939 hap57 submitting submitting submitting submitting submitting submitting

A03a Scytodidae Scytodes thoracica Turkey Alanya District,

Antalya 36.543/32.105 110 submitting submitting submitting submitting submitting submitting

L195 Scytodidae Scytodes thoracica Greece Koufovouno,

Komotini 41.353/26.453 56 submitting submitting submitting submitting submitting submitting

L198 Scytodidae Scytodes thoracica Greece Kalo Horio, Crete 35.276/25.362 101 submitting submitting submitting submitting submitting submitting

L199 Scytodidae Scytodes thoracica Greece Agios Nikolaos,

Crete 35.319/25.549 68 submitting submitting submitting submitting submitting submitting L200 Scytodidae Scytodes thoracica Greece Salakos, Rhodes 36.285/27.948 232 submitting submitting submitting submitting submitting submitting L141 Scytodidae Scytodes sp. China Baise, Guangxi N/A N/A submitting submitting submitting submitting submitting submitting L156 Scytodidae Scytodes sp. China Yizhou, Guangxi 24.505/108.665 221 submitting submitting submitting submitting submitting submitting L161 Scytodidae Scytodes sp. China Haikou, Hainan 19.952/110.565 17 submitting submitting submitting submitting submitting submitting

GRS_1 Scytodidae Dictis denticulate China Baise, Guangxi 23.996/106.233 142 submitting submitting submitting submitting submitting submitting G5 Scytodidae Dictis striatipes China Yizhou, Guangxi 24.505/108.665 221 submitting submitting submitting submitting submitting submitting

T-6 Oonopidae Ischnothyreus peltifer China Diaoluoshan,

Hainan N/A N/A submitting submitting submitting submitting submitting submitting T-2 Oonopidae Xestaspis loricata China Sanya, Hainan N/A N/A submitting submitting submitting submitting submitting submitting

Slrlyf374 Sicariidae Loxosceles rufescens China Ganzhou, Jiangxi 27.914/114.191 152 submitting submitting submitting submitting submitting submitting LPisco Sicariidae Loxosceles laeta Peru Pisco N/A GQ279223* GQ279183* N/A GQ279181* N/A N/A

Sicariidae Loxosceles mrazig GQ279179* Sicariidae Loxosceles mrazig FJ986179* KF717027* KF716985* Sicariidae Loxosceles mrazig GQ279185*

dyssilK94 Dysderidae Dysdera silvatica Canary Islands

La Gomera, San Sebastian N/A AF244273* EU139808* EU143842* EU139674* EU139739* N/A

nhm000289 Dysderidae Dysdera silvatica Canary Islands

El Hierro, Cueva de Longueras N/A JN689050*

Dysderidae Dysderocrates silvestris Croatia Zadari AF244236* AF244151* EU139755* CRBA677

Dysderidae Dysdera erythrina fervida France Iles d'Hyeres GQ285630* GQ285615* GQ285606* GQ285625*

Dysderidae Harpactocrates radulifer Spain

La Rioja,Iberian

Peninsula AF244235* EU139821* EU139753*

qradl181 Dysderidae Harpactocrates radulifer Spain Burgos, Castillay

Leon KM219561* nhm000300 Dysderidae Harpactocrates radulifer Spain La Rioja, Varganon JN689052*

Dysderidae Harpactea hombergi Spain Montseny, Iberian

Peninsula AF244233* EU139820* AF244148* EU139752* 86 Dysderidae Harpactea hombergi Belgium KF929033*

Dysderidae Rhode scutiventris Spain Iberian Peninsula EU139636* EU139822* EU139684* EU139754* Segestriidae Ariadna fidicina FJ607555* FJ607520* FJ607447* FJ607594* FJ607481* Segestriidae Segestria bavarica Germany Bonn AY560802* AY560765*

DNA100446446 Pholcidae Leptopholcus sp. Haiti Le Prete JX024078* JX023627* JX023968*

DNA100446457 Pholcidae Leptopholcus hispaniola Dominican Republic La Vega JX023558* JX023625* JX023966*

DNA100446103 Pholcidae Leptopholcus baoruco Dominican Republic Barahona JX023626* JX023967*

Plectreuridae Plectreurys tristis USA AY560768* AY560682*

hetjugLR56 Sparassidae Heteropoda jugulans Australia QLD: Daintree N/A KF442813* KF372792* KF432881* KF372702* KF442888* N/A arglob0160 Araneidae Argiope lobata Spain ASK MATJAZ N/A KJ957971* KJ958019* N/A arglob0160 Araneidae Argiope lobata N/A DQ018853* N/A arglob0160 Araneidae Argiope lobata Caribbean N/A KJ157103* KJ156988* N/A

FAPDNA003 Araneidae Zygiella x-notata EU003311* EU003431* EU003251* EU003341* EU003367* Araneidae Araneus diadematus FJ607553* FJ607518* FJ607445* FJ607592* FJ607479* Araneidae Argiope argentata FJ607554* FJ607519* FJ607446* FJ607593* FJ607480*

orsmalG16 Linyphiidae Orsonwelles malus Hawaiian

Island Kauai: Nualolo tr. N/A AY078697* AY078687* AY078795

* AY078730* AY078708* AY078676

* Atypus

heterothecus Atypidae Atypus heterothecu

s China Luohu, Shenzhen 22.578/114.170 KP208882* KP229789* N/A KP229783* KP229799* N/A Conothele

taiwanensis06 Ctenizidae Conothele taiwanensis Laos Oudom Xia

Province 20.705/101.972 KP208890* KP229788* KP208878* KP229780* N/A N/A Latouchia typica03 Ctenizidae Latouchia typica China Zhangqiu,Shandong 36.670/117.553 KP208884* KP229792* N/A KP229785* KP229797* N/A

NazeAmami 3278 Liphistiidae Heptathela amamiensis Japan Amami, Kyushu 28.363/129.502 KP229858* KP230179* KP230399* KP230039* KP229940* N/A

Fanelaos 3265 Liphistiidae Liphistius laoticus Laos Fane waterfalll,

Pakse 15.182/106.127 KP229896* KP230235* KP230289* KP230101* KP229944* N/A

Wanzhou 2256 Liphistiidae Songthela bristowei China Wanzhou, Chongqing 30.757/108.380 KP229808* KP230204* KP230324* KP230061* KP230025* N/A

* sequences from GenBank

Table A6. Primer sequences used in this study.

Gene Primers code Primer sequence Reference

COI LCO1490 5΄-GGT CAA CAA ATC ATA AAG ATA TTG G-3΄ Folmer et al. 1994

HCO2198

5΄-TAA ACT TCA GGG TGA CCA AAA AAT

CA-3΄ Folmer et al. 1994

CO1jerry 5΄-CAA CAT TTA TTT TGA TTT TTT GG-3΄ Folmer et al. 1994

C1N2776 5΄-CTT CTG GAT GAC CAA AAA ATC-3΄ Hedin and Maddison 2001

F42 5΄-GCT TTC CCG CGG ATG AAT AA-3΄ Present study

R675 5΄-ACG CTC GAG TAT CAA CGT CT-3΄ Present study

R847 5΄-GAA TTA GCC AAG ATC ACA CCT GT-3΄ Present study

H3 H3aF 5′-ATGGCTCGTACCAAGCAGACVGC-3′ Colgan et al. 1998

H3aR 5′-ATATCCTTRGGCATRATRGTGAC-3′ Colgan et al. 1998

16S 16S1471-mod 5΄-GCC TGT TTA WCA AAA ACA T-3΄ Crandall and Fitzpatrick 1996

16Sbr-H-mod 5΄-CCG GTY TGA ACT CAR ATC AYG T-3΄ Palumbi et al. 2002

16SA 5΄-CGC CTG TTT ATC AAA AAC AT-3΄ Tan et al. 1999

16SB2 5΄-CTC CGG TTT GAA CTC AGA TCA-3΄ Tan et al. 1999

18S 5F 5′-GCG AAA GCA TTT GCC AAG AA-3′ Giribet et al. 1999

9R 5′-GAT CCT TCC GCA GGT TCA CCT AC-3′ Giribet et al. 1999

28S 28S"O" 5΄-GAA ACT GCT CAA AGG TAA ACG G-3΄ Hedin and Maddison 2001

28S"C" 5΄-GGT TCG ATT AGT CTT TCG CG-3΄ Hedin and Maddison 2001

ZX1 5΄-ACC CGC TGA ATT TAA GCA TAT-3΄ Bond and Hedin 2006

A58OP1 5΄-AGA GCC AAT CCT TGT CCC GA-3΄ Bond and Hedin 2006

Internal primers for sequencing

65-w1r 5΄-GGC AGG TGA GTT GTT ACA CA-3΄ Present study

ITS2 CAS5p8SFC

5΄-TGA ACA TCG ACA TTT YGA ACG CAC

AT-3΄ Ji et al. 2003

CAS28SB1d

5΄-TTC TTT TCC SCT TAY TRA TAT GCT

TAA-3΄ Ji et al. 2003

Supplemental references：

Folmer, O. et al. 1994. DNA primers for amplification of mitochondrial cytochrome

coxidase subunit I from diverse metazoan invertebrates. Mol. Mar. Biol.

Biotechnol., 3: 294–299.

Crandall, K. A. and Fitzpatrick, J. E. 1996. Crayfish molecular systematics: using a

combination of procedures to estimate phylogeny. Syst. Biol., 45: 1–26.

Palumbi, S. R. et al. 2002. The simple fool’s guide to PCR, Version 2.0. Honolulu:

privately published.

Tan, A. M. et al. 1999. Paraphyly of the Enoplognatha group (Araneae, Theridiidae)

based on DNA sequences. J. Arachnol., 27: 481–488.

Bond, J. E. and Hedin, M. C. 2006. A total evidence assessment of the phylogeny of

North American euctenizine trapdoor spiders (Araneae, Mygalomorphae,

Cyrtaucheniidae) using Bayesian inference. Mol. Phylogenet. Evol., 41: 70–85.

Ji, Y. J. et al. 2003. Evolutionary conservation and versatility of a new set of primers

for amplifying the ribosomal internal transcribed spacer regions in insects and other

invertebrates. Mol. Ecol. Notes, 3: 581–585.

Hedin, M. C. and Maddison, W. P. 2001. A combined molecular approach to

phylogeny of the jumping spider subfamily Dendryphantinae (Araneae: Salticidae).

Mol. Phylogenet. Evol., 18: 386–403.

Giribet, G. et al. 1999. Internal phylogeny of the Chilopoda (Myriapoda, Arthropoda)

using complete 18S rDNA and partial 28S rDNA sequences. Philos. Trans. R. Soc.

London, Ser. B-Biol. Sci., 354: 215–222.

Colgan, D. J. et al. 1998. Histone H3 and U2 snRNA DNA sequences and arthropod

molecular evolution. Aust. J. Zool., 46: 419–437.

Table A7. Parameters of the best-fitting substitution model for each codon base or

gene partition selected under the Akaike information criterion (AIC). COI-1, COI-2

and COI-3 represent the first, second and third base of each codon of COI gene,

respectively; H3-1, H3-2 and H3-3 represent the first, second and third base of each

codon of H3 gene, respectively.

gene/codon base model partition -InL p AIC deltaAIC weight CumWeight

16S GTR+I+G 012345 10027.9544 420 20895.9089 0.0 0.9999 1

18S K80+I+G 010010 1464.7743 413 3755.5485 0.0 0.3217 0.322

28S GTR+I+G 012345 7342.3087 420 15524.6174 0.0 0.8064 0.806

ITS2 GTR+G 012345 6091.2903 419 13020.5807 0.0 0.876 0.876

COI-1 GTR+I+G 012345 2792.4608 420 6424.9217 0.01 0.9999 1

COI-2 GTR+I+G 012345 1041.4467 420 2922.8934 0.0 0.9995 1

COI-3 GTR+I+G 012345 11390.5325 420 23621.065 0.0 0.5141 0.514

H3-1 GTR+I 012345 372.3733 419 1582.7467 0.0 0.5411 0.541

H3-2 JC 000000 141.1392 410 1102.2783 0.0 0.2961 0.296

H3-3 GTR+I+G 012345 2173.9995 420 5187.9991 0.0 0.6276 0.628

Table A8. Divergence times of major Araneidea and Haplogynae taxa based on fossil

calibration under uncorrelated Lognormal relaxed clock. The main in-group dating

times of Stedocys were shown in Figure S1B. 95%HPD is 95% highest posterior

density. Ma is million years ago.

Taxa Fossil calibration (Prior distribution = Normal) Median (95%HPD) (Ma) Scytodes crown Initial value = 33.9, mean = 36, stdev = 1.2 36.81 (34.51–39.10) Loxosceles crown Initial value = 13.82, mean = 17, stdev = 1.9 19.48 (16.13–22.76) Dysderidae crown Initial value = 35, mean = 37, stdev = 1.2 37.46 (35.23–39.78) New word Leptopholcus crown Initial value = 20, mean = 30, stdev = 6.0 21.92 (12.91–32.46) Haplogynae crown Initial value = 173, mean = 173, stdev = 0.01 173 (172.98–173.02) Araneoidea stem Initial value = 176, mean = 176, stdev = 0.01 176 (175.98–176.02)

ecography ecog-02908 · 2017. 2. 2. · x084 scytodidae stedocys sp. 17 china hechi, guangxi n/a...

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