ecoguard(oil-degrading microbial agent)english

8/9/2019 EcoGuard(Oil-Degrading Microbial Agent)English

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Ecophile Co., Ltd


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Items Our product Other product Remark

1. Microbes mixMix by hydrocarbon

intake mechanism

Mix by oil-degrading

capacity

Harmony with native microbes

(Environment-friendly )

2. Feasibility of

weathered oil

Adding the microbesdegrading metabolite,

Adding the microbesdegrading lubricant

-Excellent feasibility in chroniccontaminated soil and lubricant

contaminated soil

3. Condition of

large scale culture

Adding thehydrocarbon

Adding generalnutrients

Excellence of initial oil-degrading velocity

Characteristics


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The products are officially certified by the MOE (Ministry of Environment) and KCG

(Korea Coast Guard) on efficiency & safety


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EfficiencyEfficiency--increasing technology by hydrocarbon uptake mechanism of oilincreasing technology by hydrocarbon uptake mechanism of oil--degrading microbesdegrading microbes

0

0. 2

0. 4

0. 6

0. 8

1

1. 2

1. 4

1. 6

0 20 40 60 80 100

ydrophobicity %

EmulsifyingActi

ity

D

at610nm

WL- 1

WL

- 1

WL

- 2

WL

- 3

WL- 2

DJ-1

DJ-3

L180

K

3- 2

DJ-2

YS- 7

- 10

0

0. 2

0. 4

0. 6

0. 8

1

1. 2

1. 4

1. 6

0 20 40 60 80 100

ydrophobicity %

EmulsifyingActi

ity

D

at610nm

WL- 1

WL

- 1

WL

- 2

WL

- 3

WL- 2

DJ-1

DJ-3

L180

K

3- 2

DJ-2

YS- 7

- 10


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Group 1 Group 2 Group 3 Group 4

Group 1

Group 2 23 5

Group 3 4

41

Group 4 9

91 93

Division

trains CharacteristicUptakeMode

Group 1

H-2,3

DJ-3EA o, HP q

Pseudo-solubilized

Group 2

-1, 2 EA q, HP oDirectContact

Group 3

H3-2, DJ-2 EA q, HP q

olubilized

Group 4

H-1, DJ-1

C

1

, IC-1

-7

MIXED MIXED


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Adding of metaboliteAdding of metabolite--degrading microbesdegrading microbes

-- TwoTwo microbes deposit (oversea) ,microbes deposit (oversea) , -- Patent pending/registrationPatent pending/registration

!

+"

2

+ N/P +

!

+"

2

+ N/P +

Biotrans formation

(Acids ,#

etons ,Alco hols ..)

Biotrans formation

(Ac ids ,#


$

es id% e s

(:!

& clics , PA

s ..)

$

es id% e s

( :!

& clics , PA

s ..)

'

ineralization

! "

2 +

2

"

'

inera lizatio n

! "

2 +

2

"BiomassBiomas s

Fee dback

Inhibition

!

+"

2

+ N/P +

!

+"

2

+ N/P +

Biotrans formation

(Acids ,#


Biotrans formation

(Ac ids ,#


$

es id% e s

(:!

& clics , PA

s ..)

$

es id% e s

( :!

& clics , PA

s ..)

'

ineralization

! "

2 +

2

"

'

inera lizatio n

! "

2 +

2

"BiomassBiomas s

Fee dback

Inhibition


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SA AR

IS

SA AR

IS

SA AR

IS

ASP

SA AR

IS

ASPA) Initial

(

) After 18 days


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FinalFinal oiloil--degradingdegrading raterate ((1616 days)days))

A 0 R)

A 0 R ((5959..2211

))

InIn thethe casecase ofof metabolitemetabolite--degradingdegrading microbesmicrobes adding,adding, TPHTPH concentrationconcentration waswas reducedreduced asas

averageaverage 566566ppmppm everydayeveryday..

CostCost reductionreduction byby shortenshorten treatmenttreatment periodperiod


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Test itemsTest items UnitsUnits Standard valueStandard value Result valueResult value RemarkRemark

OilOil--degrading capacitydegrading capacity

(TPH)(TPH)mg/kgmg/kg

Soil Environment Conservation2

awSoil Environment Conservation2

awee

3

a3

a 44 regionregion

(2

ess than 2,000mg/kg)(2

ess than 2,000mg/kg)

2

ess than 600mg/kg2

ess than 600mg/kg

5

orea resources5

orea resources

corporationcorporation

Measurement o 4 totalMeasurement o 4 total

microbes3

o.microbes3

o.CFU/mlCFU/ml > 10> 1088 2.4 X 102.4 X 101010

5

orea Testing and5

orea Testing and

Research InstituteResearch Institute

Measure o 4 oilMeasure o 4 oil--degradingdegrading

microbes3

o.microbes3

o.MP

3

/mlMP3

/ml 5X105X1077 ~ 10~ 1088 5.2 X 105.2 X 1099 SelfSelf--test resulttest result


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After sampling, suspension

according to each dilution

rate

Measurement of total

microbial6

o.:

Spread on the agar plate

Oil-degrading microbial6

o.:

Inoculated on 96 well micro

plate

- Total microbial6

o :

Cultured on 30 for 3days

- Oil-degrading microbial6

o :

Cultured on 30 for 10days

Total microbial6

o. :

Colony6

o.

(Units : CFU/g-soil)

Oil-degrading microbial6

o :

( : MP6

/g-soil)Oil-degrading activity is indicated by red color.


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Sample extraction Sample sonication Sample Cleaning Sample

concentration

Adding internal

standard

Sample7

oading Data analysis Results


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(A) Sample roading, (8

) Sample developing, (C) Evaporation of solution,(D) Rod hold, (E) Analysis screen, (F) T

9

C/FID system

Fig. T9

C/FID analysis of fractionated resin componentseparated by modified method

Primary developing

Secondary developing

Tertiary developing

Developing solution: n-hexaneAliphatic : 10cm developing

Developing solution : n-hexane : toluene(1:4)Aromatic : 5cm developing

Developing solution :dichloromethane:methanol(19:1)Resin developing


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\ \ \


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0

10

20

30

40

50

60

70

80

90

Diesel-contaminated soil

Crude oil-contaminated soil

Lubricant oil-contaminated soil

Crude oil-contaminated soil (the coast)

0

10

20

30

40

50

60

70

80

90

100

Ga region Na region Da region

A Co. (Domestic)

F Co. (Foreign)

EcoGuard


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Division Contents Remark

Development

periods

-Ministry of Environment (G7 Project, @ 92. ~ c 02)

(A

ORDI, Technology transfers)

-Ministry of Environment (Eco-technopia 21 , @ 01 ~ @ 09)

-Ministry of Commerce (Project, @ 00 ~ @ 02)

-Ministry of Science an @ Technology (Project, @ 01 ~ @ 02)

Development costs\1,620,000,000

(A

ORDI @ evelopment costs : \2,200,000,000)

A

orean 100B

Manpower 8 personnel (Total 65 personnel input)

Designer 2 personnel (Facility @ esign)

TechnologyC

evelopment

ecoguard(oil-degrading microbial agent)english

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