editorial promotions for life scientists...

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Promotions for Life Scientists ISSUE 13 - MAY 2009 VALID UNTIL 31ST AUGUST 2009 CHECK OUT WWW.VWR.COM FOR FULL SPECIFICATIONS DE Editor VWR International Europe bvba Researchpark Haasrode 2020 Geldenaaksebaan 464 3001 Leuven Belgium Copywriting VWR International Layout and typesetting Marketing Services VWR Printing Stork, Germany No part of this publication may be reproduced or copied without prior permission by writing of VWR International Europe. Run 77 000 copies Publication date: May 2009 Due to the high sales volume of promoted articles some items may be temporarily out of stock - VWR Terms and Conditions of Sale apply. http://de.vwr.com/lifescience ISSUE 13 - MAY 2009 WWW.VWR.COM Local price agreements apply. EDITORIAL IN THIS ISSUE Welcome to the first edition of the VWRbioMarke shop of 2009. This year is a very exciting year for our BDH Prolabo brand – the BDH part is now 100 years old! We were very proud to merge BDH and Prolabo brands in 2007 combining the best quality and price competitive characteristics of the existing range yet still retain our molecular biology grade basics that have proven so useful to life scientists. Throughout the year, watch out for special promotions as we celebrate this very special event - our first product is water - simple yet indispensable. One amongst our many great offers in this issue - enjoy! Electrophoresis ClearPAGE pre-cast gels from CBS Molecular Biology BDH Prolabo mol. biol. grade water VWR Collection DOPPIO and UNO cyclers CapitalBio Luxscan 10K PerfeCTa qPCR Supermixes from Quanta Fast Plasmid mini kit from 5Prime Applichem DNA-ExitusPlus TM IF E.Z.N.A. TM gel extraction kit from Omega Biotek BTX 96 well electroporation Proteomics Complete your chromatography tool box - Pall Life Sciences Avoid over-biotinylation and confirm sufficient labelling without time consuming assays - SoluLinK ProteaseArrest™ inhibitor cocktails - G biosciences GE illustra products for sample preparation - GE Healthcare Easy to use custom peptide online order tool! - Thermo Scientific Liquid Handling Be ready for any dispensing challenge! - Thermo Scientific New ART ® Pipette Tips Reload System- Molecular Bioproducts Sample Preparation Wheaton CRIMPenstein™ electric crimper/decapper Spectrum Tube-A-Lyzer™ Cell Biology Trust Corning for laboratory miniaturisation and automation - Corning BD Falcon™ individual cell culture inserts BRAND plates ® Nunc cell culture flasks Nalgene aPES filter units Electrophoresis ClearPAGE™ precast gels Try these gels and experience the technology yourself! Unique acrylamide polymer for exceptional protein or DNA separations Sharp, clean, consistently straight bands with unsurpassed resolution Incredibly strong and elastic gels won’t tear or crack Simple buffer system - a single buffer system is used for all SDS gels Lower anode buffer can be recycled to save time and money Minimum 1 year shelf life with no deterioration of performance No trimming of gel is required for blotting in these units 2 gels can be electrophoresed under identical running conditions 10 x 10 cm cassette design results in functionally longer path length than competitors Well fingers extend above cassette and are anchored so that they remain vertical for easy loading of sample Coloured beads in loading area provide easier well visualisation FREE unit The DCX-700 is a dual function, mini-vertical slab gel/blotting system in one unit with capacity for two runs at a time. It has a unique design that makes it easy to use, ensures great results and a fast throughput of samples. For more information on these revolutionary gels see the VWRbioMarke Magazine 22. Gel choices: Type Single % Gradient % Well format SDS 8, 10, 12, 16 4-8, 4-12, 4-20, 10-20 12, 17, 2D, 1 DNA/Native 10 2-8, 3-20 12, 17 Special promotion kit includes: DCX-700 with blotting cassettes & freezer blocks 50 gels of your choice either SDS or DNA/Native, but NOT a combination 500 ml (20X) Running Buffer 10 ml (4X) Sample Buffer 1 ml (10X) DTT Reducer To benefit from this promotion contact us at [email protected] Molecular Biology Over 100 years of excellence – BDH Prolabo chemicals Our fantastic bag-in-box packaging for the molecular biology grade water means that you can buy in an affordable 10 l pack and be assured that the inner bag will collapse as the product is used keeping every drop as fresh as the first. DNase, RNase and protease free. Description Pack Size Cat. No. Price Promo Price € Molecular biology grade water 10 l 445847DP 45,50 25,00 Ultimate PCR flexibility! The UNO cycler is a 96 or 384 format gradient cycler with standard or FAST blocks- your choice! The DOPPIO accommodates two independent 48 well gradient blocks both with sprung heated lids that adapt to both tubes and high profile 48 well plates. DOPPIO on it’s own is already an incredible price but this super combo gives you all the choices you could need! Description Cat. No. Price € Promo price € UNO & DOPPIO thermal cycler Combo 732-1200PROP 7805,00 5823,00 96 well standard block for UNO Cycler 732-1203P 1785,00 96 well FAST block for UNO Cycler 732-1205P 3081,00 384 well standard block for UNO Cycler 732-1204P 2112,00 384 well FAST block for UNO Cycler 732-1206P 3407,00 UNO thermal cycler 732-1200P 4040,00 DOPPIO thermal cycler 732-1210P 3565,00 The microarray scanner that you can afford! CapitalBio Luxscan TM 10K A dual channel confocal microarray scanner This compact high performance scanner system for microarray imaging and data analysis of DNA, protein and cell arrays, integrates many advanced optical, signal processing and motion control systems. LuxScan™ 10K has two excitation lasers (532 nm and 635 nm) and provides high detection sensitivity, accuracy, dynamic range and linearity. Advanced and optimised signal acquisition and processing elements, mean it is an ideal tool for profiling microarray-based gene expression assays. *Software and other training not included - this is a chargeable extra depending on specific requirements promo code 1 Limit of 1 kit per customer Promotion runs January 2009 – December 2009. So if you don’t need one now - keep this for later! promo code 3 promo code 4 promo code 2

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Page 1: EDIToRIAL Promotions for Life Scientists Electrophoresisde.vwr-cmd.com/ex/downloads/life_science/biomarke/... · ultimate PCR flexibility! The uNO cycler is a 96 or 384 format gradient

Promotions for Life Scientists

ISSUE 13 - MAY 2009 • VALID UNTIL 31ST AUGUST 2009 • CHECK OUT WWW.VWR.COM FOR FULL SPECIFICATIONS

DE

EditorVWR International Europe bvbaResearchpark Haasrode 2020Geldenaaksebaan 4643001 LeuvenBelgium

CopywritingVWR International

Layout and typesettingMarketing Services VWR

PrintingStork, Germany

No part of this publication may be reproduced or copied without prior permission by writing of VWR International Europe.

Run77 000 copiesPublication date: May 2009

Due to the high sales volume of promoted articles someitems may be temporarily out of stock - VWR Terms andConditions of Sale apply.

http://de.vwr.com/lifescience

ISSuE 13 - MAY 2009

www.vwR.Com

Local price agreements apply.

EDIToRIAL

In ThIs IssuE

Welcome to the first edition of the VWRbioMarke shop of 2009. This year is a very exciting year for our BDH Prolabo

brand – the BDH part is now 100 years old! We were very proud to merge BDH and Prolabo brands in 2007 combining the best quality

and price competitive characteristics of the existing

range yet still retain our molecular biology grade basics that have proven so useful to life scientists. Throughout the year, watch out for special promotions as we celebrate this very special event - our first product is water - simple yet indispensable.

One amongst our many great offers in this issue - enjoy!

ElectrophoresisClearPAGE pre-cast gels from CBS•

molecular BiologyBDH Prolabo mol. biol. grade water•VWR Collection DOPPIO and uNO cyclers•CapitalBio Luxscan• ™ 10KPerfeCTa qPCR Supermixes from Quanta•Fast Plasmid mini kit from 5Prime•Applichem DNA-ExitusPlus• TM IFE.Z.N.A.• TM gel extraction kit from Omega BiotekBTX 96 well electroporation•

ProteomicsComplete your chromatography tool box •- Pall Life SciencesAvoidover-biotinylationandconfirm•sufficientlabellingwithouttimeconsuming assays - SoluLinKProteaseArrest™ inhibitor cocktails - •G biosciencesGE illustra products for sample •preparation - GE HealthcareEasy to use custom peptide online order •tool!-ThermoScientific

Liquid handlingBe ready for any dispensing challenge! - •ThermoScientificNew ART• ® Pipette Tips Reload System- Molecular Bioproducts

sample PreparationWheaton CRIMPenstein™ electric •crimper/decapperSpectrum Tube-A-Lyzer™•

Cell BiologyTrust Corning for laboratory •miniaturisation and automation - CorningBD Falcon™ individual cell culture inserts•BRAND plates• ®

Nunccellcultureflasks•NalgeneaPESfilterunits•

ElectrophoresisClearPAGE™ precast gels Try these gels and experience the technology yourself!

unique acrylamide polymer for exceptional protein or DNA separations•Sharp, clean, consistently straight bands with unsurpassed resolution•Incredibly strong and elastic gels won’t tear or crack•Simple buffer system - a single buffer system is used for all SDS gels•Lower anode buffer can be recycled to save time and money•Minimum 1 year shelf life with no deterioration of performance•No trimming of gel is required for blotting in these units •2 gels can be electrophoresed under identical running conditions•10 x 10 cm cassette design results in functionally longer path length than competitors•Well fingers extend above cassette and are anchored so that they remain vertical for easy loading of sample•Coloured beads in loading area provide easier well visualisation•

FREE unitThe DCX-700 is a dual function, mini-vertical slab gel/blotting system in one unit with capacity for two runs at a time. It has a unique design that makes it easy to use, ensures great results and a fast throughput of samples. For more information on these revolutionary gels see the VWRbioMarke Magazine 22.

Gel choices:Type single % Gradient % well format

SDS 8, 10, 12, 16 4-8, 4-12, 4-20, 10-20 12, 17, 2D, 1

DNA/Native 10 2-8, 3-20 12, 17

special promotion kit includes:DCX-700 with blotting cassettes & freezer blocks•50 gels of your choice either SDS or DNA/Native, •but NOT a combination500 ml (20X) Running Buffer•10 ml (4X) Sample Buffer•1 ml (10X) DTT Reducer•

To benefit from this promotion contact us at [email protected]

molecular Biologyover 100 years of excellence – BDh Prolabo chemicalsOur fantastic bag-in-box packaging for the molecular biology grade water means that you can buy in an affordable 10 l pack and be assured that the inner bag will collapse as the product is used keeping every drop as fresh as the first. DNase, RNase and protease free.

Description Pack size

Cat. no. Price €

Promo Price €

Molecular biology grade water 10 l 445847DP 45,50 25,00

ultimate PCR flexibility!The uNO cycler is a 96 or 384 format gradient cycler with standard or FAST blocks- your choice! The DOPPIO accommodates two independent 48 well gradient blocks both with sprung heated lids that adapt to both tubes and high profile 48 well plates.

DoPPIo on it’s own is already an incredible price but this super combo gives you all the choices you could need!

Description Cat. no. Price € Promo price €

uNO & DOPPIO thermal cycler Combo 732-1200PROP 7805,00 5823,0096 well standard block for uNO Cycler 732-1203P 1785,0096 well FAST block for uNO Cycler 732-1205P 3081,00384 well standard block for uNO Cycler 732-1204P 2112,00384 well FAST block for uNO Cycler 732-1206P 3407,00uNO thermal cycler 732-1200P 4040,00DOPPIO thermal cycler 732-1210P 3565,00

The microarray scanner that you can afford! CapitalBio LuxscanTm 10K A dual channel confocal microarray scanner

This compact high performance scanner system for microarray imaging and data analysis of DNA, protein and cell arrays, integrates many advanced optical, signal processing and motion control systems. LuxScan™ 10K has two excitation lasers (532 nm and 635 nm) and provides high detection sensitivity, accuracy, dynamic range and linearity. Advanced and optimised signal acquisition and processing elements, mean it is an ideal tool for profiling microarray-based gene expression assays.

*Software and other training not included - this is a chargeable extra depending on specific requirements

promo code

1

Limit of 1 kit per customerPromotion runs January 2009 – December 2009. so if you don’t need one now - keep this for later!

promo code

3

promo code

4

promo code

2

Page 2: EDIToRIAL Promotions for Life Scientists Electrophoresisde.vwr-cmd.com/ex/downloads/life_science/biomarke/... · ultimate PCR flexibility! The uNO cycler is a 96 or 384 format gradient

Place your order today! visit our website http://de.vwr.comLocal price agreements apply.

offers valid from 1st may to 31st August 2009Promotions in the VWRbioMarke SHOP may not be used in combination, VWR reserves the right to refuse invalid orders.2

ISSuE 13 - MAY 2009

molecular BiologyExpand your genotyping projects and contain costs with PerfeCTa qPCR supermixes Quanta Biosciences qPCR SuperMixes give superior accuracy at one-half the primer and probe concentration meaning you can run twice as many samples with your valuable assays.

PerfeCTa qPCR SuperMixes are optimised for use on any real-time instrument. Select Low-ROX (ABI 7500), ROX (all other ABI Instruments) or no reference dye.

Description Pack Cat. no. Price €

PerfeCTa qPCR SuperMix 100 733-1183P 156,00PerfeCTa qPCR SuperMix 500 733-1184P 743,00PerfeCTa qPCR SuperMix ROX 100 733-1187P 156,00PerfeCTa qPCR SuperMix ROX 500 733-1188P 743,00PerfeCTa qPCR SuperMix Low-ROX 100 733-1191P 156,00PerfeCTa qPCR SuperMix Low-ROX 500 733-1192P 743,00

DnA-ExitusPlus™ IFDNA-ExitusPlus™ IF is AppliChem’s novel patented decontamination solution for the removal of DNA and RNA contamination on instruments and surfaces such as PCR workstations, electrophoresis equipment, pipettes, reaction tubes etc.

DNA-ExitusPlus™ IF doesn’t contain aggressive mineralic acids or alkaline substances. All components of the solution are readily biologically degradable and not harmful or toxic to humans. Catalytic and cooperative effects of DNA-ExitusPlus™ IF cause a very rapid non-enzymatic degradation of nucleic acids.

Description Pack Cat. no. Price €

DNA-ExitusPlus™ IF 500 ml A7409.0500P 38,60

Test of the DNA degradation potential for selected conventional DNA decontamination reagents (X1 – X4) in comparison with DNA- ExitusPlus™ IF (D+). Only DNA- ExitusPlus™ permits complete DNA removal.

Plasmid DnA in just 9 minutes A winning combination for plasmid DnA mini and maxi preps

5 PRImE FastPlasmid mini Kit gets results in just 9 minutes. This innovative technology provides sequencing-grade plasmid DNA in half the time of an alkaline lysis kit - perfect for screening for positive clones.

how much plasmid DnA do you need?The 5 PRImE PerfectPrep EndoFree maxi Kit enables purification of up to 1000 µg of ultrapure plasmid DNA in under one hour. Efficient endotoxin removal ensures that the resulting plasmid DNA is ideally suited for demanding applications, including transfection of sensitive mammalian cells.

Description Preps Cat. no. Price €

FastPlasmid Mini Kit - 100 100 733-0175P 121,00FastPlasmid Mini Kit - 250 Including Free PerfectPrep EndoFree Maxi Kit 250 733-0176P 270,00

PerfectPrep EndoFree Maxi Kit 100 733-1942P 133,00

see the Advantage of BTX 96 well Electroporation Fast library development •Simplified method optimisation for high yields•no need for expensive kits•

The BTX 25 or 96 well HTS system is a powerful tool for optimising experimental variables in minutes. Run 48 samples in duplicate and in minutes be ready to analyse the results; for example, five different cell densities, three Nucleic acid concentration, eight different buffers and up to 8 varied electrical conditions in just minutes. The BTX HT systems allow you to get more data rapidly, optimise for highest yields and save time without expensive reagent kits. Run 96 wells of duplicates 48 samples for fast library development in minutes.

Description no. of wells

Gap (mm)

Cat. no. Price €

HT 830/200 System 732-0344P 17760,00HT 630/200 System 732-0475P 15140,00HT-P96-4 96 4 732-0477P 341,00HT-P96-2 96 2 732-0476P 341,00

Complete your chromatography tool box new Acrosep chromatography column chemistries from Pall Life sciences - for ion exchange, affinity, and mixed-mode chromatography applications.

The Acrosep columns offer:True scale - up - the same resin chemistry •for small-scale discovery to biopharmaceutical manufacturingVersatile - luer inlet and outlet for use with syringe, pump or automated •chromatography systemHigh binding capacities, fast flow rates•Colour coded and labelled by chemistry type •Hexagonal collar so columns will not unexpectedly roll off lab surface.•

Find out more at www.pall.com/acrosep

1 ml Acrosep column type Qty/Pk Cat. no. Price € Promo Price €

HyperD F CM 5 569-1000P 69-1000 88,00 74,80HyperD F S 5 569-1001P 1001 88,00 74,80HyperD F Q 5 569-1002P 88,00 74,80HyperD F DEAE 5 569-1003P 88,00 74,80PROTEIN A 5 569-1004P 416,00 353,60IMAC 5 569-1008P 110,00 93,50SDR HyperD 5 569-1010P 145,00 123,25Blue Trisacryl M 5 569-1009P 127,00 107,951 ml Ion Exchange AcroSep Variety 4 555-0086P 87,50 74,35

Purchase an E.Z.n.A.™

Gel Extraction kit

The E.Z.N.A.® Gel Extraction kit uses HiBind®

DNA spin-column technology to recover DNA bands 100 bp - 20 kb in length from all grades of agarose gel with yields up to 85%. The whole process can be completed in less than 15 minutes. The E.Z.N.A.® Gel Extraction kits can also used to purify DNA fragments from PCR products and enzymatic digestions.

Description Pack Cat. no. Price €

Gel Extraction kit Q spin 50 OMEGD2501-01P 50,80Gel Extraction kit Q spin 200 OMEGD2501-02P 176,00Gel Extraction kit v spin 50 OMEGD2500-01P 52,00Gel Extraction kit v spin 200 OMEGD2500-02P 183,00

Avoid over-biotinylation and confirm sufficient labelling without time consuming assays The ChromaLink™ Biotin labelling kit uses biotin labelling reagent with built-in signal traceability to rapidly calculate the exact number of biotins attached to a protein or antibody. ChromaLink™

ensures your results will always be clear and precise.

Biotinylate and quantify conjugated •biotin with one kitEasy to use - all protocols are on usB •stick

Description Cat. no. Price € Promo Price €

ChromaLink Biotin labelling kit 733-2500P 311,00 233,25

promo code

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Proteomicspromo

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Page 3: EDIToRIAL Promotions for Life Scientists Electrophoresisde.vwr-cmd.com/ex/downloads/life_science/biomarke/... · ultimate PCR flexibility! The uNO cycler is a 96 or 384 format gradient

Place your order today! visit our website http://de.vwr.comLocal price agreements apply.

offers valid from 1st may to 31st August 2009Promotions in the VWRbioMarke SHOP may not be used in combination, VWR reserves the right to refuse invalid orders. 3

ISSuE 13 - MAY 2009

ProteomicsProteaseArrest™

inhibitor cocktails

General protease inhibitor cocktail of reversible and irreversible inhibitors of serine, cysteine, calpain, and metalloproteases, that achieves >95% inhibition.

An optional EDTA solution is provided separately as it would inhibit proteins that require divalent cations or protein purification using immobilised metal affinity chromatography (IMAC).

Suitable for the protection of proteins from animal tissues, plants, yeast, and bacteria.

Description size Cat. no. Price €

ProteaseArrest™ inhibitor cocktails 100X concentrated, ready to use solutionProteaseArrest™ 2 ml 733-1943P 166,00OneQuant™ ProteaseArrest™ (24 single aliquots for 10 ml buffer) 24 x 100 µl 733-1944P 179,00

FOCuS™ ProteaseArrest™ (2D electrophoresis compatible cocktail, alternative metalloprotease inhibitor) 1 ml 733-1945P 169,00

Species specific ProteaseArrest™ inhibitor cocktails (dry format) Bacterial ProteaseArrest™ [100X] 2 ml 733-1946P 173,00Mammalian ProteaseArrest™ [100X] 2 ml 733-1947P 173,00Plant ProteaseArrest™ [100X] 2 ml 733-1948P 173,00Yeast ProteaseArrest™ [100X] 2 ml 733-1949P 173,00ProteCEAsE™ inhibitor cocktailsProteCEASE™-50, with EDTA 10 vials, 1 vial for 50 ml buffer 733-1950P 205,00ProteCEASE™-50, with EDTA 20 vials, 1 vial for 50 ml buffer 733-1951P 409,00ProteCEASE™-50, without EDTA 10 vials, 1 vial for 50 ml buffer 733-1952P 205,00ProteCEASE™-50, without EDTA 20 vials, 1 vial for 50 ml buffer 733-1953P 409,00ProteCEASE™-100, with EDTA 10 vials, 1 vial for 100 ml buffer 733-1954P 407,00ProteCEASE™-100, without EDTA 10 vials, 1 vial for 100 ml buffer 733-1955P 407,00

Easy to use custom peptide online order tool! Test how easy it is to order Thermo Scientific peptides on vwR.com. Mention the code vwR39 when placing your order and win one of our 2 GB-uSB Sticks.*

1. Go to VWR.com and enter your account.2. Click on the biopolymers configurator link “Order Thermo Biopolymers” to configure your peptides.3. Finish your order on the VWR lab shop.

Contact your local vwR Lifesciences specialist for more information. * Dissemination is at the entire

discretion of Thermo Fisher Scientific GmbH

GE illustra products for sample preparation selection guide for protein and nucleic acid sample preparation.

Liquid handlingBe ready for any dispensing challenge!

The Thermo Scientific Multidrop Combi reagent dispenser offers unrivalled levels of performance, versatility and simplicity for pharmaceutical and biotech laboratories.

The Thermo Scientific FILLit software provides increased functionality and the SMART feature enhances QC procedures.

Description Cat. no. Price €

Multidrop Combi SMART with FILLit software 735-0288P 13 689,00

new ART® Pipette Tips Reload system The only Aerosol Barrier Tip available that guarantees 100% protection against aerosol and liquid contamination of your sample and pipette is now available in environmentally friendly packaging.

Hinged rack with flip lid offers flexibility by •allowing the option of a hinged rack or lift-off lidPatented interactive barrier prevents •aerosols and aqueous based solutions passing throughReload insert packed in a blister tub •for easy insertion Safe lock latch provides ergonomic •one-handed opening and closingCertified free of RNase, DNase, ATP •and Pyrogens

Supplier in pack of 10 racks of 96 tips each

ART barrier tips in hinged Rack packs:

Type vol. (µl) Cat. no. Price € (1 pack)

Price for 3 packs including free tips

ART 10 10 732-1139P 138,00 414,00ART 10 REACH 10 732-1140P 138,00 414,00ART 20E 10 732-1141P 138,00 414,00ART 20P 20 732-1142P 138,00 414,00ART 100 100 732-1143P 138,00 414,00ART 100E 100 732-1144P 138,00 414,00ART 200 200 732-1145P 138,00 414,00ART 1000 1000 732-1146P 171,00 513,00

sample Preparationwheaton CRImPenstein™ electric crimper/decapper

A new and innovative piece of laboratory equipment to save time and make work easier

This lightweight, ergonomically designed unit enables handheld semi-automatic process for extended period of time. CRIMPenstein™ is offered with quick change jaw-sets available in 8, 11, 13 and 20 mm sizes, with the easily regulated jaw action providing the perfect crimp. Eliminates the need to purchase separate crimper and decapper.

CRImPenstein™ unit and Power Cord*

Jaw set size (mm) Cat. no. Price € Promo price €

8 548-0139P 1610,00 1288,0011 548-0148P 1610,00 1288,0013 548-0170P 1610,00 1288,0020 548-0180P 1610,00 1288,00

CRImPenstein™ stand

Description Cat. no. Price €

CRIMPenstein™ Stand 548-0137P 260,00

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Full range of vials available -

please enquire

great value

Page 4: EDIToRIAL Promotions for Life Scientists Electrophoresisde.vwr-cmd.com/ex/downloads/life_science/biomarke/... · ultimate PCR flexibility! The uNO cycler is a 96 or 384 format gradient

Place your order today! visit our website http://de.vwr.comLocal price agreements apply.

offers valid from 1st may to 31st August 2009Promotions in the VWRbioMarke SHOP may not be used in combination, VWR reserves the right to refuse invalid orders.4

ISSuE 13 - MAY 2009

order from the Customer serviceCentre or your vwR Primary Distributor:These offers cannot be used in conjunction with anyother pricing agreements from VWR International.Prices exclude VAT.

VWR International GmbHHilpertstr. 20A64295 Darmstadt

Place your order today!Tel: 0180-570 2000*Fax: 0180-570 2222*http://de.vwr.come-Mail: [email protected]*14 Cent/Minute aus d. dt. Festnetzggf. abweichende Mobilfunktarife

sample Preparation BRAnDplates®

Tissue culture-treated (sterile)96 well plates are available with u, V, F or C-Bottom•96 well standard plates bear a coloured •alphanumeric code (TC plates orange)BRANDplates• ® are non cytotoxic according to ISO 10993, free of endotoxins (< 0.01 E.u./ml), and free of DNA, DNase and RNase. Sterile Products comply with ISO 11137 and AAMI guidelines. A •SAL of 10-6 is reached. Supplied individually wrapped with lid.Packed 50 to a case•

cellGrade™ :• standard surface for adherent cell linescellGrade™ plus:• recommended for more fastidious adherent cell lines, enhanced cell attachment and growthcellGrade™ premium: • nonbiological surface that imitates Poly-D-Lysine for weakly adherent cells (primary cells, HEK, HepG2)

384 versions also available on promotion, please enquire

Descriptionwell shape/volume(µl)

Approx. growth area (cm2)

Cat. no. Price € Promo price

cellGrade™96 Well, Clear u/330 0.30 735-2097P 107,40 75,1096 Well, Clear V/350 0.30 735-2098P 107,40 75,1096 Well, Clear F/350 0.31 735-2099P 107,40 75,1096 Well, Clear C/350 0.25 735-2100P 107,40 75,1096 Well, White F/350 0.31 735-2101P 200,00 140,0096 Well, Black F/350 0.31 735-2102P 200,00 140,0096 Well, White/Clear F/330 0.31 735-2103P 489,50 342,0096 Well, Black/Clear F/330 0.31 735-2104P 489,50 342,00cellGrade™ plus96 Well, Clear F/350 0.31 735-2116P 117,90 82,5096 Well, White F/350 0.31 735-2117P 220,00 154,0096 Well, Black F/350 0.31 735-2118P 220,00 154,0096 Well, White/Clear F/320 0.31 735-2119P 515,80 361,0096 Well, Black/Clear F/320 0.31 735-2120P 515,80 361,00cellGrade™ premium96 Well, Clear F/350 µl 0.31 735-2124P 208,50 145,0096 Well, White F/350 µl 0.31 735-2125P 373,70 261,0096 Well, Black F/350 µl 0.31 735-2126P 373,70 261,0096 Well, White/Clear F/320 µl 0.31 735-2127P 756,80 529,0096 Well, Black/Clear F/320 µl 0.31 735-2128P 756,80 529,00

Thermo scientific nunc cell culture flasks The Nunc™ range of cell culture flasks offers consistent quality and the guarantee of best reproducibility in cell culture research. They are made of highly characterised,virginpolystyrenewiththepatentedNunclon™∆surface modified for excellent attachment and proliferation of cells.

QC tested with 4 different cell lines to ensure unfailing •monolayer formation and consistent cloning efficiencyVolume graduations on both sides•Nunc certified uSP Class VI, gamma •sterile (SAL 10-6) and non-pyrogenic

Standard Flask with Nunclon™∆ Surface

Culture area (cm2)

neck style Closure Qty/Pk Cat. no. Price €

80 straight Filter 50 734-2131P 70,7480 straight Vent/close 50 734-2046P 69,52

EasYFlask™ with Nunclon™∆ Surface Culture area (cm2)

neck style Closure Qty/Pk Cat. no.

Price €

175 angled Filter 30 734-2167P 76,17175 angled Vent/close 30 734-2168P 75,09

Many other formats and sizes are available, please enquire

Thermo scientific nalgene aPEs filter units new 0.1 µm pore size protects from mycoplasma contaminationThe Nalgene® range of MF75™ Filters with Supor® machV PES membrane for the cold sterilisation of all your liquid cell culture media and sera. The asymmetric PES membrane gives very high filtration rates and high throughput but ensures that your valuable proteins are not retained by the membrane.

Non-cytotoxic, non-pyrogenic & •radiation sterilised filter units and receiver bottlesProduct and lot identification printed •on the upper part for full traceability, full certificate in every boxRNase/DNase-free, 5-year shelf life•Leakproof receiver bottles, preventing •pH-shift of stored solutions

size (ml)

membrane Dia. (mm) Qty/Pk Cat. no. Price €

Filter unit, 0.1 µm aPEs membrane – nEw150 50 12 514-0612P 70,52250 50 12 514-0614P 78,54500 75 12 514-0613P 127,00Conical filter tube, 0.2 µm aPES membrane50 50 12 514-0590P 79,99Filter unit, 0.2 µm aPEs membrane115 50 72 514-0023P 54,12150 50 12 514-0024P 70,52250 50 12 514-0027P 78,54500 75 12 514-0025P 127,00500 90 12 514-0028P 150,661000 90 12 514-0026P 161,81Bottle top filter, 33 mm neck, 0.2 µm aPES500 75 12 514-0029P 73,93150 50 12 514-0030P 73,931000 90 12 514-0031P 52,83Bottle top filter, 45 mm neck, 0.2 µm aPES500 75 12 514-0032P 52,83150 50 12 514-0033P 141,551000 90 12 514-0034P 141,55

Trust Corning for laboratory miniaturisation and automationCorning offers a complete range of 96 well assay microplates – sBs standard

General assays – not treated, NBS™, covalent binding, high •bind, flexible vinyl (PVC), and uV microplates Cell-based assays – tissue culture treated, Corning• ® CellBIND® Surface, poly-D-lysine, and ultra low attachment polystyrene microplates Immunoassays – EIA/RIA polystyrene plates (medium and high binding) •supplied in packs of 100•

Assay plate, 96 well, polystyrene, flat bottom

Colour sterile Cat. no. Price €

Medium binding Clear No 732-5567P 170,07TC-treated, with lid Clear Yes 734-1611P 209,35High binding Clear No 732-5568P 176,36Non treated, no lid White No 734-1662P 299,24High binding, no lid White No 734-1666P 333,24Non treated, no lid Black No 734-1663P 359,35High binding, no lid Black No 734-1667P 333,24

Cell BiologyBD Falcon™ individual cell culture inserts

Clear PET membranesBroad range of pore sizes:- High density 0.4 or 1.0 µm PET membranes: for a maximum diffusion into, out of, and through cell monolayers - 1.0 and 3.0 µm membranes: best suited for establishing barrier function models, investigating cellular

migration, and studying transport of larger molecules (e.g., lipoproteins)- 8.0 µm membranes: useful for studying migration, chemotaxis, and tumor invasion/metastasisBD Falcon™ FluoroBlok™ cell culture inserts- Fluorescence blocking membranes

BD Falcon™ Individual Cell Culture Inserts used in conjunction with Multiwell Companion Plates enable the evaluation of many experimental conditions simultaneously.All supplied in packs of 48

BD Falcon™ individual cell culture inserts (6 well format)

Pore size (µm) Density Cat. no. Price €

0.4LD Transparent 734-0032P 241,00HD Translucent 734-0061P 241,00

1.0 Transparent 734-0040P 241,00

3.0LD Transparent 734-0033P 241,00HD Translucent 734-0034P 241,00

8.0 Transparent 734-0035P 241,00

BD Falcon™ individual cell culture inserts (12 well format)

Pore size (µm) Density Cat. no. Price €

0.4LD Transparent 734-0051P 189,00HD Translucent 734-0062P 189,00

1.0 Transparent 734-0041P 189,00

3.0LD Transparent 734-0052P 189,00HD Translucent 734-0060P 189,00

8.0 Transparent 734-0053P 189,00

BD Falcon™ and BD Falcon™ FluoroBlok™

individual cell culture inserts (24 well format)

Pore size (µm) Density Cat. no. Price €

0.4LD Transparent 734-0036P 160,00HD Translucent 734-0063P 160,00

1.0Transparent 734-0042P 160,00BD FluoroBlok™ 734-0369P 299,00

3.0LD Transparent 734-0037P 160,00HD Translucent 734-0397P 160,00BD FluoroBlok™ 734-0370P 299,00

8.0Transparent 734-0038P 160,00BD FluoroBlok™ 734-0371P 299,00

The First Disposable & Ready-to-use Dynamic-Dialysis Device for Fragile samples Tube-A-Lyzer™ is a self-contained ready to use mass transfer system that incorporates a semi-permeable, tubular dialysis membrane inside a clear reservoir housing separating the sample chamber from the surrounding buffer chamber. This revolutionary disposable dialysis device

designed is ideal for a rapid yet gentle dialysis and separation of larger sample volumes; exhibiting higher efficiency as well as product recovery.

supplied in packs of 3

sample volume

mwCo (Dalton)

Cat. no. Price € Promo price €

8 – 10 ml.

100 – 500 734-1447P 214,00 171,203 500 – 5 000 734-1448P 214,00 171,208 000 – 10 000 734-1449P 214,00 171,2020 734-1450P 214,00 171,2050 734-1451P 214,00 171,20100 734-1452P 214,00 171,20

25 – 30 ml.

100 – 500 734-1453P 231,00 184,803 500 – 5 000 734-1454P 231,00 184,808 000 – 10 000 734-1455P 231,00 184,8020 734-1456P 231,00 184,8050 734-1457P 231,00 184,80100 734-1458P 231,00 184,80

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