electronic supporting information · web viewbo dai,a,1 shujing chen,a,1 wei li,a,1 lulu zheng,a...

Supplementary Material Fully-functional semi-automated microfluidic immunoassay platform for quantitation of multiple samples Bo Dai, a,1 Shujing Chen, a,1 Wei Li, a,1 Lulu Zheng, a Xiaodian Han, b Yongfeng Fu,* c Jiandong Wu, d Francis Lin, d Dawei Zhang,* a and Songlin Zhuang a a Engineering Research Center of Optical Instrument and System, the Ministry of Education, Shanghai Key Laboratory of Modern Optical System, University of Shanghai for Science and Technology, Shanghai, 200093, China. b Department of Laboratory Medicine, Shanghai Cancer Center, Fudan University, Shanghai, 200032, China c Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China. S1

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Page 1: Electronic Supporting Information · Web viewBo Dai,a,1 Shujing Chen,a,1 Wei Li,a,1 Lulu Zheng,a Xiaodian Han,b Yongfeng Fu,*c Jiandong Wu,d Francis Lin,d Dawei Zhang,*a and Songlin

Supplementary Material

Fully-functional semi-automated microfluidic immunoassay

platform for quantitation of multiple samples

Bo Dai,a,1 Shujing Chen,a,1 Wei Li,a,1 Lulu Zheng,a Xiaodian Han,b Yongfeng Fu,*c

Jiandong Wu,d Francis Lin,d Dawei Zhang,*a and Songlin Zhuang a

a Engineering Research Center of Optical Instrument and System, the Ministry of Education,

Shanghai Key Laboratory of Modern Optical System, University of Shanghai for Science and

Technology, Shanghai, 200093, China.

b Department of Laboratory Medicine, Shanghai Cancer Center, Fudan University, Shanghai,

200032, China

c Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences,

Fudan University, Shanghai, 200032, China.

d Department of Physics and Astronomy, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada

*Corresponding authors:

E-mail: [email protected] (D. Zhang); [email protected] (Y. Fu)

Page 2: Electronic Supporting Information · Web viewBo Dai,a,1 Shujing Chen,a,1 Wei Li,a,1 Lulu Zheng,a Xiaodian Han,b Yongfeng Fu,*c Jiandong Wu,d Francis Lin,d Dawei Zhang,*a and Songlin

Fig. S1. The algorithm of the image processing for establishing the standard curve and

calculating the concentration of the samples. sTrans and yTrans are the transmittance for

standards and unknowns in each well. sAbs and yAbs are the absorbance for standards and

unknowns. sBlank and yBlank are the transmittance in the blank well. sCal and yCal are the

absorbance for standards and unknowns after the calibration by deducting the

absorbance in the blank well.

Page 3: Electronic Supporting Information · Web viewBo Dai,a,1 Shujing Chen,a,1 Wei Li,a,1 Lulu Zheng,a Xiaodian Han,b Yongfeng Fu,*c Jiandong Wu,d Francis Lin,d Dawei Zhang,*a and Songlin

Fig. S2. (a) The design of the stopper. (b) and (c) The photos of the stopper when the

rod is lifted upwards and pulled downwards.

Page 4: Electronic Supporting Information · Web viewBo Dai,a,1 Shujing Chen,a,1 Wei Li,a,1 Lulu Zheng,a Xiaodian Han,b Yongfeng Fu,*c Jiandong Wu,d Francis Lin,d Dawei Zhang,*a and Songlin

Fig. S3. Images captured by the AMI. Test 1, Test 2 and Test 3 are the assays of the

standards. Test 4, Test 5 and Test 6 are the assays of 33 clinical samples.

Page 5: Electronic Supporting Information · Web viewBo Dai,a,1 Shujing Chen,a,1 Wei Li,a,1 Lulu Zheng,a Xiaodian Han,b Yongfeng Fu,*c Jiandong Wu,d Francis Lin,d Dawei Zhang,*a and Songlin

Fig. S4. The response of the reaction product from the assays conducted in the 384-

well plate and the microfluidic chip. The absorbance of the three color channels of the

camera (the red, blue and green solid curves) is plotted against the optical density

obtained by the commercial plate reader (the black dashed curve). The incubation time

is 120 minutes and VChip=20 μL.

Page 6: Electronic Supporting Information · Web viewBo Dai,a,1 Shujing Chen,a,1 Wei Li,a,1 Lulu Zheng,a Xiaodian Han,b Yongfeng Fu,*c Jiandong Wu,d Francis Lin,d Dawei Zhang,*a and Songlin

Table S1 The parameter values for LOD calculation

96-well plate Microfluidic chipA 0.060 4.325B 2.443 193.659C 107.917 39.480D –1.357 –1.406

yBlank 0.062 4.877❑Blank 0.002 0.310LOD 1.349 1.262

Table S2 The absorbance of the blue channel of the camera for the assays in Fig. S3

Sample1 2 3 4 5 6 7 8 9 10 11

Test 1 5 19 29 57 105 144 176 189 6 5 5Test 2 6 21 31 62 99 143 173 191 5 6 6Test 3 5 19 28 58 102 142 170 187 7 5 5Test 4 25 60 160 55 110 24 171 50 53 56 62Test 5

139 145 130 169 72 51 130 142 27 70180

Test 665 22 66 150 135 20 23 127 49 138

169

Table S3 The concentration (pg/mL) of the clinical samples in Fig. S3

Sample1 2 3 4 5 6 7 8 9 10 11

Test 46.79

18.93

101.50

17.1242.5

76.43

134.49

15.36