elisa tests
TRANSCRIPT
ELISAvirology
Hamid Afkhami
EL Enzyme-LinkedEnzyme catalysis the substrate and produce colored species.
IS ImmunoSorbentAntigen or antibody are sticked in wells
About ELISA
Types of the ELISA1. Direct2. Indirect3. Sandwich4. competitive
Direct ELISASTEPS:
Antigen stick to well with charge + casein or albumin(BSA).
Add sample to well Wash Add substrate acid measure OD
Advantages Quick Eliminate Cross-
reactivity of secondary Ab
Disadvantages the signal is less
amplified Labeling has destructive
effects on ab and consuming time.
Direct ELISA
STEPS: Antigen stick to well with
charge + casein or albumin(BSA).
Add sample + Wash well Add detector ab + wash Add substrate and
measure OD
Indirect ELISA
Advantages: High sensitivity Flexibility This format of indirect ELISA is
suitable for determining total antibody level in samples
Cost saving Several Epitopes for secondary
Ab and signal amplification
Indirect ELISA
Antigen detection STEPS:
Stick ab to well + BSA Add sample (ag) + wash Add detector ab + wash Add substrate + measure OD
Advantages High specificity Suitable for complex samples
SANDWICH ELISAcontain at least two antigenic
COMPETITIVE ELISA
COMPETITIVE ELISA
Advantages Suitable for complex samples Flexibility and sensitivity
COMPETITIVE ELISA
http://www.elisa-antibody.com/ELISA-Introduction https://www.bio-rad-antibodies.com https://www.youtube.com http://arian-andish.com https://en.wikipedia.org/wiki/ELISA
Sources
Have a nice day