Mechatronics Project

EMMET SystemElectroMechanical Membrane-Enabled

Transfer System

Carlo YuviencoKeeshan Williams

Alberto Giacomello

C. Yuvienco; K. Williams; A. GiacomelloEMMET System

Process

Objective: ProteinPurification

Present Implementation

C. Yuvienco; K. Williams; A. GiacomelloEMMET System

C. Yuvienco; K. Williams; A. GiacomelloEMMET System

C. Yuvienco; K. Williams; A. GiacomelloEMMET System

C. Yuvienco; K. Williams; A. GiacomelloEMMET System

Vallejo, L. F. & Rinas, U. Strategies for the recovery of active proteins through refolding of bacterial inclusion body proteins. Microb Cell Fact 3, 11, doi:10.1186/1475-2859-3-11

The Electrode SurfaceC. Yuvienco; K. Williams; A. Giacomello

EMMET System

The Electrode Surface –Equivalent Circuit

C. Yuvienco; K. Williams; A. GiacomelloEMMET System

C. Yuvienco; K. Williams; A. GiacomelloEMMET System

Our Proposal

C. Yuvienco; K. Williams; A. GiacomelloEMMET System

1. Digital level sensor2. Reservoir3. Power strip4. Signal generator5. Reservoir valve

(servo controlled)6. µ controller and circuit boards7. Reaction vessel8. Stir bar

Primary System Components

C. Yuvienco; K. Williams; A. GiacomelloEMMET System

1. Digital level sensor2. Reservoir3. Power strip4. Signal generator5. Reservoir valve

(servo controlled)6. µ controller and circuit boards7. Reaction vessel8. Stir bar

Primary System Components

C. Yuvienco; K. Williams; A. GiacomelloEMMET System

1. Digital level sensor2. Reservoir3. Power strip4. Signal generator5. Reservoir valve

(servo controlled)6. µ controller and circuit boards7. Reaction vessel8. Stir bar

Primary System Components

C. Yuvienco; K. Williams; A. GiacomelloEMMET System

1. Digital level sensor2. Reservoir3. Power strip4. Signal generator5. Reservoir valve

(servo controlled)6. µ controller and circuit boards7. Reaction vessel8. Stir bar

Primary System Components

Current Source

C. Yuvienco; K. Williams; A. GiacomelloEMMET System

ElectrodeInstrumentation

Amplifier

Micro Controller

Valve Actuator

Full Wave Rectifier

VACI

A2D Converter

VDC

C. Yuvienco; K. Williams; A. GiacomelloEMMET System

User Interface and Usage1. User is prompted to input 3-4 parameters that defines the

sensitivity and operation of the system2. System reports measured voltage differential and status of

valve and reservoir liquid level3. System is automated to control and maintain the

concentration gradient of solutes across the membrane

C. Yuvienco; K. Williams; A. GiacomelloEMMET System

Advantages• saving on time, materials, manpower• enables a precise control of the process• completely automatizable, customizable• dyalisis may follow desired protocols

Future Improvements• use of Ion Selective Electrodes• improvement of the user interface• concentration readings on LCD