entering the lab (a fresh beginning) -...
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Entering the lab (A fresh beginning)
Andrei Leitão
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Types of pipets
Pasteur Transfer Volumetric Serological Mohr
To contain (TC) To deliver (TD)
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Types of pippetors Micropippetor
Manual Electronic Robotics
Electronic pippetor
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Flasks Beaker Erlenmeyer Graduated cylinder Buret Volumetric
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Electronic balances
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Small tissue culture laboratory
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Laminar flow hood
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Liquid dispensers
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Common light microscopes
Magnification: Resolution:
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CO2 incubator
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Sterilizing the material Vertical autoclave Horizontal autoclave
Sterilizing oven Bunsen burner
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Microplate readers - spectrometers
Colorimetry Fluorescence Fluorescence polarization Luminescence Microscopy
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Probability of contamination
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Reading material
Gallagher, S.R.; Wiley, E.A. “Current Protocols in Essential Laboratory Techniques” Volume/Weight Measurement Reagent Preparation Cell Culture Techniques Freshney, Y. “Culture of animal cells” Laboratory Design, Layout, and Equipment Equipment and Materials Aseptic Technique Safety, Bioethics, and Validation
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Planning the assay
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