epithelium and junctions vibs 443/602. em 3: region of basal lamina and connective tissue beneath...
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Epithelium and Junctions
VIBS 443/602
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EM 3: region of basal lamina and connective tissue beneath intestinal epithelial absorptive cells
1. Plasma membrane
2. Basal lamina
3. Connective tissue
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EM 17; capillary in the lamina propria of the intestine (duodenum); 13,500x
1. Smooth muscle cell
2. Capillary
3. Endothelial cell
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Renin granules in Kidney (PAS)
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Ileum, monkey (PAS)249
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Trachea, monkey
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Esophagus and trachea, monkey – glands in trachea
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Larynx (Gallego's stain)
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SPECIALIZATION OF EPITHELIA
MAINTAIN EXTENSIVE
CONTACTS AMONG CELLS
STRUCTURALLY AND FUNCTIONALLY POLARIZED
JUNCTIONSZONULA OCCLUDENS - TIGHT
JUNCTION (BELT)
ZONULA ADHERENS –
ADHERING BELT
DESMOSOME (MACULA
ADHERENS) - SPOT ATTACHMENT
GAP JUNCTIONS - COMMUNICATION
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Terminal bars
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32409
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EM 6b: Region showing zonula occludens, zonula adherents, and macula adherents junctions between cells.
1. Zonula occludens
2. Zonula adherens
3. DesmosomeEM 6b
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EM 6b
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EM 6c
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EM 4: Continuity between junctional complex and terminal web
1. Terminal web
2. Interdigitation of plasma membrane
3. Terminal bar
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EM 4a: Ridges and grooves of zonula occludens visualized by freeze cleave microscopy
1. Zonula occludens
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Inside the cell Outside the cell
P = Protoplasmic side E = Ectoplasmic side
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EM 4a
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EM 2a: Particles and pits of gap junction visualized by freeze cleave microscopy
1. Gap junction
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EM 2a
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Triad with bile duct Liver & spleen with colloidal carbon, rat
118
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EM 2: portion of two cells including gap junction
1. Desmosome
2. Gap junction
3. Rough endoplasmic reticulum
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absorptive cells of Ileum, monkey250
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Ileum, monkey (PAS)
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argentaffin cells of Ileum, monkey
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Large intestine or Colon, monkey
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EM 4c: intestinal absorptive cell (super nuclear region); 60,000x
1. Budding RER
2. Coated vesicle
3. Golgi
4. Mitochondria
5. Nucleus
6. Plasma membrane
7. Primary lysosome
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Endothelial cells lining small blood vessel lumen with smooth muscle cells and fibrolasts in the vessel wall.
1. Lumen
2. Endothelial cell
3. Fibrolast
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EM 10a: Endothelial cells lining capillary with pericyte in the vessel wall.
1. Lumen
2. Endothelial cell
3. Tight junction
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Skeletal muscle in spermatic cord UT 196
196
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UT196 valves in large vein of spermatic cord
195
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EM 10f: arteriolar wall; 30,000x
1. Apical caeolae
2. Collagen
3. Dense bodies
4. Elastic fiber
5. Endothelial cell
6. Nuclear pore
7. RER
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EM 8h: macrophage in testis; 30,000x.
1. Enlarged basal lamina
2. Heterophagic vacuole
3. Leydig cell cytoplasm
4. Nucleolus
5. Myoid cell
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Skin abdomen
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Skin, scalp
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Skin hand monkey109
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Skin hand monkey
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Skin111
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Skin, foot
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Vagina
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EM 8g: epidermis; 7,000x. Note desmosomal connections between cells
1. Keratinocyte
2. Desmosome
3. Melanocyte
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EM 16: surface mucosa of stomach; 13,500x
1. Granules of surface mucous cells
2. Mitochondria
3. Nuclei
4. Lumen
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Urinary bladder, monkey160
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Ureter – transitional epithelium and smooth muscle
262
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Penis – transitional epithelium and surrounding spongy cavernous of penal urethra
277
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Penis - transitional epithelium of penal urethra 277
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