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  • See discussions, stats, and author profiles for this publication at: https://www.researchgate.net/publication/235681814

    ERG induces androgen receptor-mediated regulation of SOX9 in prostate cancer

    Article in The Journal of clinical investigation · March 2013

    DOI: 10.1172/JCI66666 · Source: PubMed

    CITATIONS

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    15 authors, including:

    Changmeng Cai

    University of Massachusetts Boston

    50 PUBLICATIONS 1,418 CITATIONS

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    Sen Chen

    Beth Israel Deaconess Medical Center

    29 PUBLICATIONS 873 CITATIONS

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    Qianben Wang

    The Ohio State University

    64 PUBLICATIONS 4,116 CITATIONS

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    Myles Brown

    Harvard University

    272 PUBLICATIONS 23,664 CITATIONS

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    All content following this page was uploaded by Changmeng Cai on 08 March 2017.

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    https://www.researchgate.net/publication/235681814_ERG_induces_androgen_receptor-mediated_regulation_of_SOX9_in_prostate_cancer?enrichId=rgreq-faafcb728a074c1ceda99ace6eba2cbe-XXX&enrichSource=Y292ZXJQYWdlOzIzNTY4MTgxNDtBUzoxMDE0OTI1OTM3OTA5ODlAMTQwMTIwOTEyODgzNQ%3D%3D&el=1_x_2&_esc=publicationCoverPdf https://www.researchgate.net/publication/235681814_ERG_induces_androgen_receptor-mediated_regulation_of_SOX9_in_prostate_cancer?enrichId=rgreq-faafcb728a074c1ceda99ace6eba2cbe-XXX&enrichSource=Y292ZXJQYWdlOzIzNTY4MTgxNDtBUzoxMDE0OTI1OTM3OTA5ODlAMTQwMTIwOTEyODgzNQ%3D%3D&el=1_x_3&_esc=publicationCoverPdf https://www.researchgate.net/?enrichId=rgreq-faafcb728a074c1ceda99ace6eba2cbe-XXX&enrichSource=Y292ZXJQYWdlOzIzNTY4MTgxNDtBUzoxMDE0OTI1OTM3OTA5ODlAMTQwMTIwOTEyODgzNQ%3D%3D&el=1_x_1&_esc=publicationCoverPdf https://www.researchgate.net/profile/Changmeng_Cai?enrichId=rgreq-faafcb728a074c1ceda99ace6eba2cbe-XXX&enrichSource=Y292ZXJQYWdlOzIzNTY4MTgxNDtBUzoxMDE0OTI1OTM3OTA5ODlAMTQwMTIwOTEyODgzNQ%3D%3D&el=1_x_4&_esc=publicationCoverPdf https://www.researchgate.net/profile/Changmeng_Cai?enrichId=rgreq-faafcb728a074c1ceda99ace6eba2cbe-XXX&enrichSource=Y292ZXJQYWdlOzIzNTY4MTgxNDtBUzoxMDE0OTI1OTM3OTA5ODlAMTQwMTIwOTEyODgzNQ%3D%3D&el=1_x_5&_esc=publicationCoverPdf https://www.researchgate.net/institution/University_of_Massachusetts_Boston?enrichId=rgreq-faafcb728a074c1ceda99ace6eba2cbe-XXX&enrichSource=Y292ZXJQYWdlOzIzNTY4MTgxNDtBUzoxMDE0OTI1OTM3OTA5ODlAMTQwMTIwOTEyODgzNQ%3D%3D&el=1_x_6&_esc=publicationCoverPdf https://www.researchgate.net/profile/Changmeng_Cai?enrichId=rgreq-faafcb728a074c1ceda99ace6eba2cbe-XXX&enrichSource=Y292ZXJQYWdlOzIzNTY4MTgxNDtBUzoxMDE0OTI1OTM3OTA5ODlAMTQwMTIwOTEyODgzNQ%3D%3D&el=1_x_7&_esc=publicationCoverPdf https://www.researchgate.net/profile/Sen_Chen2?enrichId=rgreq-faafcb728a074c1ceda99ace6eba2cbe-XXX&enrichSource=Y292ZXJQYWdlOzIzNTY4MTgxNDtBUzoxMDE0OTI1OTM3OTA5ODlAMTQwMTIwOTEyODgzNQ%3D%3D&el=1_x_4&_esc=publicationCoverPdf https://www.researchgate.net/profile/Sen_Chen2?enrichId=rgreq-faafcb728a074c1ceda99ace6eba2cbe-XXX&enrichSource=Y292ZXJQYWdlOzIzNTY4MTgxNDtBUzoxMDE0OTI1OTM3OTA5ODlAMTQwMTIwOTEyODgzNQ%3D%3D&el=1_x_5&_esc=publicationCoverPdf https://www.researchgate.net/institution/Beth_Israel_Deaconess_Medical_Center?enrichId=rgreq-faafcb728a074c1ceda99ace6eba2cbe-XXX&enrichSource=Y292ZXJQYWdlOzIzNTY4MTgxNDtBUzoxMDE0OTI1OTM3OTA5ODlAMTQwMTIwOTEyODgzNQ%3D%3D&el=1_x_6&_esc=publicationCoverPdf https://www.researchgate.net/profile/Sen_Chen2?enrichId=rgreq-faafcb728a074c1ceda99ace6eba2cbe-XXX&enrichSource=Y292ZXJQYWdlOzIzNTY4MTgxNDtBUzoxMDE0OTI1OTM3OTA5ODlAMTQwMTIwOTEyODgzNQ%3D%3D&el=1_x_7&_esc=publicationCoverPdf https://www.researchgate.net/profile/Qianben_Wang?enrichId=rgreq-faafcb728a074c1ceda99ace6eba2cbe-XXX&enrichSource=Y292ZXJQYWdlOzIzNTY4MTgxNDtBUzoxMDE0OTI1OTM3OTA5ODlAMTQwMTIwOTEyODgzNQ%3D%3D&el=1_x_4&_esc=publicationCoverPdf https://www.researchgate.net/profile/Qianben_Wang?enrichId=rgreq-faafcb728a074c1ceda99ace6eba2cbe-XXX&enrichSource=Y292ZXJQYWdlOzIzNTY4MTgxNDtBUzoxMDE0OTI1OTM3OTA5ODlAMTQwMTIwOTEyODgzNQ%3D%3D&el=1_x_5&_esc=publicationCoverPdf https://www.researchgate.net/institution/The_Ohio_State_University?enrichId=rgreq-faafcb728a074c1ceda99ace6eba2cbe-XXX&enrichSource=Y292ZXJQYWdlOzIzNTY4MTgxNDtBUzoxMDE0OTI1OTM3OTA5ODlAMTQwMTIwOTEyODgzNQ%3D%3D&el=1_x_6&_esc=publicationCoverPdf https://www.researchgate.net/profile/Qianben_Wang?enrichId=rgreq-faafcb728a074c1ceda99ace6eba2cbe-XXX&enrichSource=Y292ZXJQYWdlOzIzNTY4MTgxNDtBUzoxMDE0OTI1OTM3OTA5ODlAMTQwMTIwOTEyODgzNQ%3D%3D&el=1_x_7&_esc=publicationCoverPdf https://www.researchgate.net/profile/Myles_Brown?enrichId=rgreq-faafcb728a074c1ceda99ace6eba2cbe-XXX&enrichSource=Y292ZXJQYWdlOzIzNTY4MTgxNDtBUzoxMDE0OTI1OTM3OTA5ODlAMTQwMTIwOTEyODgzNQ%3D%3D&el=1_x_4&_esc=publicationCoverPdf https://www.researchgate.net/profile/Myles_Brown?enrichId=rgreq-faafcb728a074c1ceda99ace6eba2cbe-XXX&enrichSource=Y292ZXJQYWdlOzIzNTY4MTgxNDtBUzoxMDE0OTI1OTM3OTA5ODlAMTQwMTIwOTEyODgzNQ%3D%3D&el=1_x_5&_esc=publicationCoverPdf https://www.researchgate.net/institution/Harvard_University?enrichId=rgreq-faafcb728a074c1ceda99ace6eba2cbe-XXX&enrichSource=Y292ZXJQYWdlOzIzNTY4MTgxNDtBUzoxMDE0OTI1OTM3OTA5ODlAMTQwMTIwOTEyODgzNQ%3D%3D&el=1_x_6&_esc=publicationCoverPdf https://www.researchgate.net/profile/Myles_Brown?enrichId=rgreq-faafcb728a074c1ceda99ace6eba2cbe-XXX&enrichSource=Y292ZXJQYWdlOzIzNTY4MTgxNDtBUzoxMDE0OTI1OTM3OTA5ODlAMTQwMTIwOTEyODgzNQ%3D%3D&el=1_x_7&_esc=publicationCoverPdf https://www.researchgate.net/profile/Changmeng_Cai?enrichId=rgreq-faafcb728a074c1ceda99ace6eba2cbe-XXX&enrichSource=Y292ZXJQYWdlOzIzNTY4MTgxNDtBUzoxMDE0OTI1OTM3OTA5ODlAMTQwMTIwOTEyODgzNQ%3D%3D&el=1_x_10&_esc=publicationCoverPdf

  • Lysine-Specific Histone Demethylase 1 Inhibitors Control Breast Cancer Proliferation in ERα-Dependent and -Independent Manners Julie A. Pollock,† Michelle D. Larrea,‡ Jeff S. Jasper,‡ Donald P. McDonnell,‡

    and Dewey G. McCafferty*,†,§

    †Department of Chemistry and §Department of Biochemistry, Duke University, Durham, North Carolina 27708, United States ‡Department of Pharmacology and Cancer Biology, Duke University School of Medicine, Durham, North Carolina 27708, United States

    *S Supporting Information

    ABSTRACT: Lysine specific demethylase 1 (LSD1, also known as KDM1) is a histone modifying enzyme that regulates the expression of many genes important in cancer progression and proliferation. It is present in various transcriptional complexes including those containing the estrogen receptor (ER). Indeed, inhibition of LSD1 activity and or expression has been shown to attenuate estrogen signaling in breast cancer cells in vitro, implicating this protein in the pathogenesis of cancer. Herein we describe experiments that utilize small molecule inhibitors, phenylcyclopropylamines, along with small interfering RNA to probe the role of LSD1 in breast cancer proliferation and in estrogen-dependent gene transcription. Surprisingly, whereas we have confirmed that inhibition of LSD1 strongly inhibits proliferation of breast cancer cells, we have determined that the cytostatic actions of LSD1 inhibition are not impacted by ER status. These data suggest that LSD1 may be a useful therapeutic target in several types of breast cancer; most notably, inhibitors of LSD1 may have utility in the treatment of ER-negative cancers for which there are minimal therapeutic options.

    The estrogen receptors (ER) are ligand-inducible tran-scription factors that belong to the nuclear receptor superfamily. The predominant ER subtype (ERα) is expressed in the majority of breast tumors where it enables the mitogenic actions of estrogens. As such, ERα has emerged as a major target for breast cancer therapeutics.1 Unfortunately, up to one- third of breast carcinomas lack ER at the time of diagnosis, and a fraction of cancers that are initially ER-positive apparently lose the expression of the receptor during tumor progression. The evolution of this phenotype obviously reduces the options for anti-estrogen treatment and ultimately negatively impacts survival rates. These trends are compounded by the large number of new breast cancers identified each year, predicted in 2011 to be greater than 230,000 diagnoses and 40,000 fatalities.2 Collectively these data illustrate the pressing need to understand the pathophysiology of breast cancer and to discover and exploit new targets for all forms of breast cancers. The complexity of gene regulation can be attributed not only

    to primary transcription factors but also to the large number of co-regulatory complexes that bind to and manipulate the chromatin landscape. The core histones, subject to many post- translational modifications including methylation, acetylation, phosphorylation, and ubiquitination, supply binding sites for numerous transcription factors. These modifications are present in different combinations within histones and have a powerful effect on chromatin structure. This combinatorial patterning of histone post-translational modifications influences the recruit- ment of factors that regulate gene expression, thus contributing to the “histone code” hypothesis of trancriptional regulation.3,4

    Understanding the role(s) of the enzymes involved in establishing and maintaining the histone code is an essential step in defining the role of epigenetic gene regulation in disease pathology and will be instructive with respect to new drug discovery. The likely impact of this class of targets has been highlighted by the success of inhibitors of histone deacetylases in leukem

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