escv 2008 cnrs biozone
DESCRIPTION
Présentation conjointe CRNS/Biozone Europe au congrès européen de virologie en 2008.TRANSCRIPT
Cold oxygen plasma technology efficiency against airborne respiratory viruses
Terrier O.1, Colin S. 1, VanMechelen D.2, Canu L. 2, Kurtz P. 2 , Ferraris O.1, Lina B.1 and Moules V.1
1VirPath Laboratoire de Virologie et Pathologie Humaine, CNRS FRE 3011, Université Claude Bernard Lyon 1, Faculté de Médecine Laënnec, 69372 Lyon France
2Biozone Europe, France
Cold oxygen plasma technology efficiency against airborne respiratory viruses
Terrier O.1, Colin S. 1, VanMechelen D.2, Canu L. 2, Kurtz P. 2 , Ferraris O.1, Lina B.1 and Moules V.1
1VirPath Laboratoire de Virologie et Pathologie Humaine, CNRS FRE 3011, Université Claude Bernard Lyon 1, Faculté de Médecine Laënnec, 69372 Lyon France
2Biozone Europe, France
What is plasma?
Plasma as a sterilizing agent (Menashi, 1968)
Oxygen plasma efficiency on B. subtilis and Clostridium sporogenes (Nelson and Berger, 1989)
Fourth state of matter
Plasma = ionized gas
Man-made plasma: many ways to make plasma
“Cold” plasma
Energy by radio-frequency, microwave or UV-light…
BiozoneTM technology
Cutting edge air & surface purification technology
Combination of : - Deep ultraviolet light,
- Photo-catalytic oxidation
- Cold oxygen plasma (COP)
Photo-Catalytic oxidation process destroys air pollutants on surfaces
COP eliminates reproductive capability of micro-organisms
Deep UV light sterilizes micro-organisms that pass through the machine
Negative ions decrease total concentration of harmful chemicals in the air
Ozone produced, obliterates bacteria and fungi and remove odour from the air
Airborne respiratory viruses
RSV and hPIV-3
Most important pathogens encountered in low respiratory tract infections in infants and small children (bronchiolitis and pneumonia)
Common reinfections among children and adults (Upper respiratory tract infection).
Infection transmitted by contact with infectious material and by aerosol (droplets)
Influenza
Responsible of recurrent annual epidemics causing acute febrile respiratory illness
Emerging of avian Influenza H5N1: potential threat for a pandemic
Mild to severe illness, all age group
Infection by aerosol (droplets)
Objective
Quantify the impact of deep ultraviolet light and cold oxygen plasma (COP) technologies on viral decontamination of several respiratory viruses
Classic germicide UV-light lamp
BioZoneTM UV-light Lamp (Cold 02 plasma + germicide UV)
External BioZoneTM UV-light Lamp (Cold 02 plasma only)
Human respiratory syncytial virus (RSV)
Human parainfluenza type 3 virus (hPIV-3)
Avian Influenza A/H5N2
Device
Study Design
BSL3 Lab
Viral production Purification/concentration
Viral titration (TCID50/mL)
RSV-A Long strain (ATCC VR-26)
hPIV-3 C-243 strain (ATCC VR-93)
H5N2 A/Finch/England/2051/91 strain
Upstream outlet
Downstream outlet
Nebullization Droplets
Air flow
Flow velocity 0,9m/s Reaction Time 0,44s
UV/COP Device
Results/1
1,00E+00
1,00E+01
1,00E+02
1,00E+03
1,00E+04
1,00E+05
1,00E+06
1,00E+07
1,00E+08
1,00E+09
1,00E+10 InitialUpstream
outlet
Downstream outlet
BioZoneTM
COP onlyClassic UV
No treatment
Impact of UV and cold oxygen plasma on viral decontamination of hPIV-3
TICD50/mL
Comparable effects of UV and COP (4 and 3,6 log destruction rate)
Combination of UV and COP (BioZoneTM Lamp) allow high destruction of hPIV-3: 7 log destruction rate, >99,99% in 0,44s
7 log
BioZoneTM
COP and UV
Results/2
1,00E+00
1,00E+01
1,00E+02
1,00E+03
1,00E+04
1,00E+05
1,00E+06
1,00E+07
1,00E+08
1,00E+09 InitialUpstream
outlet
Downstream outlet
No treatment
Impact of UV and cold oxygen plasma on viral decontamination of RSV
TICD50/mL
Comparable effects of UV and COP (2,1 and 2 log destruction rate)
Combination of UV and COP (BioZoneTM lamp) allow high destruction of RSV: 3,8 log destruction rate, >99,99% in 0,44s
3,8 logClassic
UVBioZoneTM
COP only
BioZoneTM
COP and UV
1,00E+00
1,00E+01
1,00E+02
1,00E+03
1,00E+04
1,00E+05
1,00E+06
1,00E+07
1,00E+08
1,00E+09
Results/3Impact of UV and cold oxygen plasma on viral decontamination of H5N2
Initial
Upstream outlet
Downstream outlet
No treatment
Classic UV
BioZoneTM
COP and UV
BioZoneTM
COP only
Comparable effects of UV and COP (3,5 and 2,9 log destruction rate)
Combination of UV and COP (BioZoneTM lamp) allow high destruction of H5N2: 5,5 log destruction rate, >99,99% in 0,44s
5,5 log
TICD50/mL
Conclusions and Perspectives
Difference of results between viruses could be explain by virus properties (structure of viral envelope) or viral suspension preparation.
High efficiency of BiozoneTM lamp in viral decontamination
Future tests with other viruses (rhinovirus, hepatitis virus…) Surface decontamination, Photocatalytic oxidation parameter
Cold oxygen plasma technology could be an innovatory and efficient way to decontaminate air to protect human populations against respiratory viruses
=> Decontamination of hospital wards, BSL3 labs…
Acknowledgements
We thank all the Staff at Centre de biologie et de Pathologie Est clinical virology lab (Lyon, France) and all the staff of VirPath lab (Bruno Lina’s group, Lyon, France)