eu fp7 cartardis project overview april 2015
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CarTarDis
Cardiovascular Target Discovery
EU FP7 Health call 2013 CarTarDis
www.cartardis.eu
Discovery of novel cardiovascular drug targets
CarTarDis = Cardiovascular Target Discovery Public-private partnership, 13 partners, 8 countries, project budget 8.0M Eur Start 1 Oct 2013 for 4 years
(Coordinator)
CarTarDis
Discovery of novel cardiovascular drug targets
(Coordinator)
CarTarDis
A strong interdisciplinary team of experts in: Cardiovascular disease biology, drug discovery processes, translational medicine, population studies, Omics, imaging, bioinformatics, applied technology
CarTarDis = Cardiovascular Target Discovery
Public-private partnership (4 years) Project budget 8.0M Eur Start 1 Oct 2013 13 partners from 8 countries: research-driven SME’s, universities, research & technology institutes, large pharma
Unique cohort and sample data CarTarDis
A global network of human cohort study data, biobanks and clinical scientists.
Unique translational CVD models CarTarDis
CVD mouse CVD patient
CVD mouse and cellular models used in >200 studies. Intensive phenotyping and molecular analyses.
Unique CVD samples CarTarDis
Unique human biobanks with samples across the cardiovascular disease spectrum.
Unique molecular detection methods CarTarDis
Immunohistochemistry (protein)
In situ hybridisation (miRNA, mRNA)
MS Imaging (lipids, metabolites)
Human/mouse CVD samples
Laser capture microdissection + targeted omics (RNA, proteins)
Histology
Rational framework for target selection
The 5R’s assessment:
• Right Target
• Right Tissue
• Right Safety
• Right Patients
• Right Commercial Potential
CarTarDis
Target identification & selection process CarTarDis
1. Generate a comprehensive candidate target database
• GWAS, literature, proprietary
2. Review all candidate targets
• Automatic scoring
• Expert review
3. Rank targets (following 5R’s)
• 1. Drugability
• 2. Novelty
• 3. Omics/genetics
• 4. Feasibility (within CarTarDis)
• 5. Pathways (expertise within CarTarDis)
4. Select shortlist of candidate targets for validation
Target View: web interface CarTarDis
http://146.107.41.240/db/CarTarDisApp/
GWAS cohort data 302
Other literature 43
Partners research 35
Total: 380
Target View: Details about each target
• Annotations
• Cross references
• Auto-collected data
• Reviewed data from
publications
• Hyperlinks to public
databases (PubMed, GWAS,
proteinatlas, etc)
• Personal comments by
experts
Ordered by ‚Target Name‘
CarTarDis
CarTarDis target reviewing process CarTarDis
• Build and improve database as central knowledge base (month 3)
• Decide on target prioritisation criteria and review procedure (month 6)
• Fill database and apply automated scoring (month 6-12)
• Discussed during various live target review jamborees (months 6, 12)
• Off line expert reviewing in small groups (email, telephone)
• Finish target selection during selection meeting (month 14)
• Prioritise targets for validation activities (month 18)
• Draft target validation plans (month 14-18)
380 candidate targets → 23 pre-selected targets → 11 selected targets
CarTarDis target evaluation dossiers CarTarDis
Knowledge sharing using Google Sites
All
selected
targets
Potential drug target
Links to
target
information
in external
databases
• Target summary - key points of pro’s and con’s of the candidate target
• Target details - description, relevant data, selection scores following 5R’s criteria
• Gap analysis - lacking data
• Validation planning - directing activities in WP1, WP2, WP3
• References - relevant literature
CarTarDis on www CarTarDis
CarTarDis website CarTarDis
www.cartardis.eu