• Exosomes are released from tumors in high abundance

• Exosome cargo reflects the origin and physiological state of the source cells

• MicroRNAs are found in high abundance in circulating exosomes

• Precipitate exosomes from patient biofluids easily

• Discover novel disease-specific biomarkers

• ExoQuick works with serum, plasma, and tumor ascites

microRNA Research

Highlights

ExoQuick™ Exosome precipitationSimple one-step solution for isolating exosomes from biofluidsRobust method for protein and microRNA biomarker discoveries

We Also O�er Custom Services

Exosomes are 40 –100 nm membrane vesicles secreted by most cell types in vivo and in vitro. Exosomes are found in blood, urine, amniotic fluid, malignant ascite fluids and contain distinct subsets of microRNAs depending upon the tumor from which they are secreted. SBI's ExoQuick exosome precipitation reagent makes microRNA biomarker discovery simple, reliable and quantitative. Enrich for circulating exosomal microRNAs with ExoQuick and accurately profile them using SBI’s SeraMir™ or QuantiMir™ qPCR arrays.

Isolate exosomes with ease

• No time-consuming ultracentrifugation

• Less expensive than costly DynaBeads

• More effective than any other method

• Use as little as 100l of serum or bio-fluid

Supe

rnat

ant

ExoQ

uick

Pelle

t

MW

mar

ker

300200

100

Nuc.

SmallRNAs

15% PA RNA Gel - SYBR green stain

Exosome RNAAnalysis

Exosome MarkerProtein Analysis

+Serum or Bio-fluid

Supernatant

Exosome pellet

ExoQuickSolution

Simple one-stepprecipitation

www.systembio.com/exoquick

Discover MicroRNAand Protein Biomarkers

in Patient Samples

Accelerating discoveries through innovationsSystem Biosciences

100nm

Data coutesy of Dr. Vladimir Baranov

kDa

55

70

3525

MW100

ExoQuick Exosome Serum Western Analysis

CD6

3

CD9C

D81

HSP

70

ExoAbs

System Biosciences offers a wide-range of custom services to support your research, allowing you to spend less time making tools, and moretime making discoveries. To learn more, visit our website at www.systembio.com/service or call us at 888-266-5066.

We Also Offer Custom Services

Isolate Exosomes from Ovarian Tumor Ascites fluid

ExoQuick™ Exosome precipitation solution

[FU]

60

50

40

30

20

10

0

25 30 35 40 45 50 55 60 65 [s]

Small RNA: 5,297.3 pg/lmicroRNA: 1,119.5 pg/lRatio: 21%

[FU]30

25

20

15

10

0

25 30 35 40 45 50 55 60 65 [s]

Small RNA: 11,116.0 pg/lmicroRNA: 5,255.4 pg/lRatio: 47%

5

Who

le A

scite

sD

ynaB

eads

[FU]30

25

20

15

10

0

25 30 35 40 45 50 55 60 65 [s]

5

ExoQ

uick

Small RNA: 16,660.5 pg/lmicroRNA: 8,904.1 pg/l

Ratio: 53%

2.0

1.6

1.2

0.8

0.4

0.0

Exos

omal

Pro

tein

(mg/

ml)

Ascites 1 Ascites 2 Ascites 3

ChromatographyDynaBeadsUltracentrifugationExoQuick

Isolate more exosomal microRNAsEnrich for more exosomal proteins

As1 As2 As3 As1 As2 As3 As1 As2 As3 As1 As2 As3Chromat. DynaBeads Ultracent. ExoQuick

Placental Alkaline Phosphatase Biomarker Enrichment

PLAP

While each method purifies exosomal material, circulating exosomes isolated by ExoQuick precipitation produces exosomal RNA and protein with greater purity and quantity than chromatography, ultracentrifugation, and anti-EpCAM magnetic DynaBeads. This will enhance the sensitivity and accuracy of down-stream analyses, such as qRT-PCR profiling of microRNA and mass spectrometric and electrophoretic analyses of exosomal proteins. Identify new diagnostic and prognostic biomarkers with ExoQuick.

Use ExoQuick to isolate exosomes from:• Patient Serum and Plasma

• Patient Tumor Ascites fluids

• Compatible with Biofluids from Any Species

• For Media and Urine - use ExoQuick-TC

microRNAs

microRNAs

microRNAs

NanoSight Particle Analysis

Particle Size (nm)0 100 200 300 400 500 600 700 800 900

90 nm

BUILD 0329 1.2 noisreV )ATN( sisylanA gnikcarT elcitraponaN

NANONANOSIGHT

2.92

x 1

0^8

part

icle

s/m

l

Time

Time

Time

Tight particle size distribution with average exosome size measured at 90nm.

• Precipitate exosomes from cell culture media or urine samples easily

• Exosomes are released from tumors in high abundance

• Exosome cargo reflects the origin and physiological state of the source cells

• MicroRNAs are found in high abundance in circulating exosomes

• Discover novel disease-specific biomarkers

microRNA Research

Highlights

ExoQuick-TC™ Exosome precipitationOptimized one-step solution for rapidly isolating exosomes from tissue culture media and urine for biomarker analysis

ustom Services

Exosomes are 60 –120 nm membrane vesicles secreted by most cell types in vivo and in vitro. Exosomes are found in blood, urine, amniotic fluid, malignant ascite fluid, tissue culture media and contain distinct subsets of microRNAs and proteins depending upon the cell-type from which they are secreted. SBI's ExoQuick-TC exosome precipitation reagent is a special polymer formulation distinct from the original ExoQuick reagent for serum. ExoQuick-TC has been optimized for exosome isolation from media and urine samples. This technology makes microRNA and protein biomarker discoveries simple, reliable and quantitative.

Isolate exosomes with ease

• No time-consuming ultracentrifugation

• Less expensive than costly DynaBeads

• More effective than any other method

• Use as little as 5ml of cell culture media or urine

www.systembio.com/exoquick

Accelerating discoveries through innovationsSystem Biosciences

ExoExoQuickQuick-TC-TC™0

0.01

0.02

0.03

0.04

0.05

0.06

0.07

0.08

0.09

0 5 10 15 20 25 30 35 40 45

Amount of Urine exosomes added (l)

OD

450

CD9

CD9 (~22 kDa)

ExoQuick-TC Urine ExosomesCD9 ELISA Assay

ExoQuick-TC precipitates urine exosomesTen milliliters of normal human urine was combined with 2ml ExoQuick-TC to precipitate urine exosomes.The exosome pellet was resuspended 175l buffer and increasing amounts of the exosome suspension was loaded onto an ELISA-ready plate. The CD9 protein was detected using SBI’s rabbit anti-CD9 p r i m a r y a n t i b o d y a n d S B I ’ s H R P - c o n j u g a t e d secondary goat anti-rabbit antibody. The size of urine CD9 proteins was determined using Western blot analysis with the same set of antibodies (see inset).

Time saving, cost-effectivesolution for studying

exosomes from culture media

Wes

tern

blo

t

R² = 0.9812

time making discoveries. To learn more, visit our website at www.systembio.com/service or call us at 888-266-5066.

ExoQuick-TC™ Exosome precipitation solution

NanoSight Analyses on Exosomes from Tissue Culture Media and Urine

1.74 x 10^9 particles/ml

133

Conc

entr

atio

n (x

10^9

/ml)

0.5

1.0

1.5

2.0

Particle Size (nm)

Screen shot of NanoSight exosomeNTA tracking data collection

Screen shot of NanoSight exosomeNTA tracking data collection

3.33 x 10^8 particles/ml

137

Conc

entr

atio

n (x

10^8

/ml)

0.75

1.5

3.0

Particle Size (nm)

Media from HT1080 cells

4.80 x 10^9 particles/ml

107

Particle Size (nm)

Conc

entr

atio

n (x

10^9

/ml)

1.2

2.4

3.6

4.8

Media from HEK293 cells

Human Urine Screen shot of NanoSight exosomeNTA tracking data collection

Human HT1080 lung sarcoma cell line was cultured in conditioned media (serum-free) for 72 hours. Ten milliliters of the media was combined with 2ml ExoQuick-TC to pellet the exosomes overnight. The exosome pellet was resuspended in 1ml PBS, diluted 1:40 and visualized on the NanoSight LM10 instrument. The analysis shows that ExoQuick isolated 133nm exosomes with a recovery of 1.74 x 10^9 particles/ml.

Human embryonic kidney (HEK) cell line was cultured in conditioned media (serum-free) for 72 hours. Ten milliliters of the media was combined with 2ml ExoQuick-TC to pellet the exosomes overnight. The exosome pellet was resuspended in 1ml PBS and visualized on the NanoSight LM10 instrument undiluted. The analysis shows that ExoQuick isolated 137nm exosomes with a recovery of 3.33 x 10^8 particles/ml.

A Normal human urine sample was used. Five milliliters was combined with 2.5ml ExoQuick-TC to pellet the exosomes overnight. The exosome pellet was resuspended in 1ml PBS, diluted 1:50 and visualized on the NanoSight LM10 instrument. The analysis shows that ExoQuick isolated 107nm exosomes with a recovery of 4.80 x 10^9 particles/ml.

Diluted 1:40

Undiluted

Diluted 1:50