explanatory words · web viewannex – part ii member state supplementary submissions under article...

ANNEX – Part II

Member State supplementary submissions under Article 54(1) of Directive 2010/63/EU – Part II

Explanatory words

This annex contains summaries of additional information, corrections and clarifications provided by Member States to complement their EU Survey submissions of 2018 on the implementation of the Directive as required in Article 54(1), and further specified in Annex I of Commission Implementing Decision 2012/707/EU. Further clarification was requested bilaterally due to missing information in the original EU Survey submission, due to unclear or incoherent information, or when likely errors were detected in these submissions.

Part II of this annex contains supplementary or corrected data that were provided in the form of additional files.

Further submissions by Member STates in the order of numbered questions in the EU Survey

C – 2.i Animals bred, killed and not used in procedures including genetically altered (GA) animals not otherwise reported in the annual statistics. This section covers animals only from the last calendar year preceding submission of information for the 5-year implementation report, that is 2017.

FR

IT

UK

D – 3.2 Provide representative information on approximate numbers, species, types of methods and their related severities of tissue sampling for the purposes of genetic characterisation carried out with and without project authorisation

AT

FR

IT

SE

UK

F – 5 Please upload here Excel template on data from 2013 to 2017

BE

BG

CZ

DK

EE

EL

FI

FR

HU

IE

LV

NL

PL

SK

UK

1

IT_C-2.i.pdf

1.Nome della specie

2.Numero animali

allevati, soppressi e non

utilizzati in procedure

esclusi quelli utilizzati

nella creazione o

mantenimento di una

linea GM

3. Numero animali

geneticamente normali

(prole wildtype) prodotti,

allevati e soppressi per la

creazione di una linea GM

4. Numero degli animali allevati

e soppressi per il mantenimento

di una linea GM stabilizzata

5. Totale degli animali allevati, soppressi e

non utilizzati in procedure e non riportati

nelle statistiche annuali (totale delle colonne

2, 3 e 4)

Topi (Mus musculus) 1 3 0 4



Pesci zebra (Danio rerio) 353 0 0 353









Ratti (Rattus norvegicus) 5 0 0 5


Altri pesci (altri Pisces) 40 0 0 40





























Polli domestici (Gallus gallus domesticus) 1170 0 0 1170



Rane (Rana temporaria e Rana pipiens) 50 0 0 50

Porcellini d India (Cavia porcellus) 55 0 0 55

















































Altri mammiferi (altri Mammalia) 191 0 0 191

















Conigli (Oryctolagus cuniculus) 17 0 0 17











Suini (Sus scrofa domesticus) 0 19 0 19



























Suini (Sus scrofa domesticus) 11 0 0 11


Criceti (Siriani) (Mesocricetus auratus) 4 0 0 4


































































Altri anfibi (altri Amphibia) 4 0 0 4








UK_C-2.i.pdf

Animal Species Specify Other Total exc

creation/maint

enance of a

GA line

Killed due

to creation

of new GA

line

killed for

maintenance

of

established

GA line

Total

[A1] Mice (Mus musculus) 0 0 141 141





[A2] Rats (Rattus norvegicus) 9 0 0 9




[A34] Zebra fish (Danio rerio) 336 0 0 336

[A17] Cattle (Bos primigenius) 4 0 0 4

[A14] Pigs (Sus scrofa domesticus) 22 0 0 22

[A16] Sheep (Ovis aries) 3 0 0 3

[A8] Rabbits (Oryctolagus cuniculus) 4 0 0 4


[A28] Domestic fowl (Gallus gallus domesticus) 617 0 0 617

[A12] Other carnivores (other Carnivora)Badger 4 0 0 4





[A11] Ferrets (Mustela putorius furo) 524 0 0 524

[A3] Guinea-Pigs (Cavia porcellus) 2596 0 0 2596





[A7] Other Rodents (other Rodentia)Mus Caroli 774 0 0 774

[A35] Other Fish (other Pisces)Atlantic salmon (Salmo salar)4778 0 0 4778

[A35] Other Fish (other Pisces)Rainbow trout (Oncorhynchus mykiss)4482 0 0 4482


[A35] Other Fish (other Pisces)Three-spined stickleback (Gasterosteus aculeatus)680 0 0 680


The annual publication on scientific procedures on living

animals includes data on sub-threshold breeding. This data

is removed when the annual data is sent to the EU each year.

It has been added in here, as requested for the additional

collection submission. Therefore, the total number

presented here will not match the total figure published in

the UK's report for the additional collection.


[A32] Xenopus (Xenopus laevis and Xenopus tropicalis) 10 0 0 10

[A35] Other Fish (other Pisces)Gadus morhua 2511 0 0 2511

[A35] Other Fish (other Pisces)Solea solea 7 0 0 7

[A35] Other Fish (other Pisces)Scophthalmus maximus 81 0 0 81

[A35] Other Fish (other Pisces)Dicentrarchus labrax 41 0 0 41

[A35] Other Fish (other Pisces)Pleuronectes platessa 1005 0 0 1005

[A35] Other Fish (other Pisces)Anguilla anguilla 1 0 0 1

[A35] Other Fish (other Pisces)Raja clavata 3 0 0 3

[A35] Other Fish (other Pisces)Syngnathus acus 1 0 0 1

[A35] Other Fish (other Pisces)Notesthes robusta 2 0 0 2

[A21] Rhesus monkey (Macaca mulatta) 4 0 0 4







[A35] Other Fish (other Pisces)Sheepshead Minnow 72 0 0 72

[A35] Other Fish (other Pisces)Rainbow Trout 100 0 0 100


[A20] Cynomolgus monkey (Macaca fascicularis) 8 0 0 8










[A19] Marmoset and tamarins (eg. Callithrix jacchus) 78 0 0 78


[A29_2] Other birds (other Aves)Duck 4 0 0 4

[A29_2] Other birds (other Aves)Bobwhite or Japanese Quail 60 0 0 60




[A10_1] Beagles (Canis lupus familiaris) 1 0 0 1



[A35] Other Fish (other Pisces)sheepshead minnow 280 0 0 280

[A35] Other Fish (other Pisces)fathead minnow 1344 0 0 1344


[A4] Hamsters (Syrian) (Mesocricetus auratus) 949 0 0 949





[A35] Other Fish (other Pisces)Atlantic salmon (Salmo salar) 525 0 0 525



[A7] Other Rodents (other Rodentia)Grey squirrel 5 0 0 5

[A35] Other Fish (other Pisces)Cyprinodon variegatus 971 0 0 971




[A35] Other Fish (other Pisces)rainbow trout 178 0 0 178

[A35] Other Fish (other Pisces)lumpfish 34 0 0 34








[A29_2] Other birds (other Aves)chicken 33 0 0 33








[A35] Other Fish (other Pisces)Trout 186 0 0 186










[A29_2] Other birds (other Aves)Gouldian Finch 4 0 0 4






[A7] Other Rodents (other Rodentia)Grasshopper 14 0 0 14


































[A21] Rhesus monkey (Macaca mulatta) 18 0 0 18







[A29_2] Other birds (other Aves)zebra finch 53 0 0 53






[A35] Other Fish (other Pisces)Pimephales promelas 1058 0 0 1058

[A35] Other Fish (other Pisces)Cyprinodon variegatus 63 0 0 63

[A35] Other Fish (other Pisces)Oncorhynchus mykiss 416 0 0 416

[A35] Other Fish (other Pisces)Cyprinus carpio 98 0 0 98





[A35] Other Fish (other Pisces)fathead minnow 1236 0 0 1236

[A35] Other Fish (other Pisces)Medaka 265 0 0 265

[A35] Other Fish (other Pisces)Rainbow trout 243 0 0 243

[A35] Other Fish (other Pisces)Lumpfish 2168 0 0 2168

[A35] Other Fish (other Pisces)Tilapia 405 0 0 405

[A35] Other Fish (other Pisces)Atlantic Salmon 832 0 0 832

[A35] Other Fish (other Pisces)Killifish 263 0 0 263


[A35] Other Fish (other Pisces)Brown trout 19 0 0 19

[A35] Other Fish (other Pisces)Bullhead 54 0 0 54

[A35] Other Fish (other Pisces)Minnow 5 0 0 5

[A35] Other Fish (other Pisces)Stoneloach 1 0 0 1







[A27] Other Mammals (other Mammalia)Laboratory opossum 91 0 0 91














[A7] Other Rodents (other Rodentia)wood mice 310 0 0 310












[A29_1] Quail (Coturnix coturnix) 2 0 0 2

[A29_2] Other birds (other Aves)Pheasant 1 0 0 1

[A29_2] Other birds (other Aves)Guinea Fowl 1 0 0 1




[A6] Mongolian gerbil (Meriones unguiculatus) 29 0 0 29

[A29_2] Other birds (other Aves)Indian runner ducks 8 0 0 8



[A29_2] Other birds (other Aves)Mallards 11 0 0 11

[A29_2] Other birds (other Aves)Aylesbury ducks 8 0 0 8


[A35] Other Fish (other Pisces)Sticklebacks 279 0 0 279



[A33] Other Amphibians (other Amphibia)Axolotl 44 0 0 44


[A7] Other Rodents (other Rodentia)Siberian Hamsters 93 0 0 93









[A35] Other Fish (other Pisces)Clarias. gariepinus 946 0 0 946


[A35] Other Fish (other Pisces)Oreochromis. aureus 90 0 0 90

[A35] Other Fish (other Pisces)Oreochromis. karongae 100 0 0 100

[A35] Other Fish (other Pisces)Oreochromis. mossambicus 191 0 0 191

[A35] Other Fish (other Pisces)Oreochromis. niloticus 2415 0 0 2415

[A35] Other Fish (other Pisces)Oryzias celebensis 10 0 0 10

[A35] Other Fish (other Pisces)Tilapia. zillii 10 0 0 10












[A35] Other Fish (other Pisces)

guppies /

Astyanax

mexicanus

294 4 0 298













[A7] Other Rodents (other Rodentia)Naked mole rat 2 0 0 2






[A35] Other Fish (other Pisces)Mixed species of Fish (Stickleback, Pulcher, Mantis Shrimp376 0 0 376
























































































[A35] Other Fish (other Pisces)Poecilia reticulata 16349 0 0 16349

[A35] Other Fish (other Pisces)Three spined stickleback 283 0 0 283


[A35] Other Fish (other Pisces)Fathead Minnow 34 0 0 34

[A35] Other Fish (other Pisces)Goodied 44 0 0 44

[A35] Other Fish (other Pisces)Knifefish 77 0 0 77


[A35] Other Fish (other Pisces)European Seabass 163 0 0 163

[A35] Other Fish (other Pisces)Arabian Killifish 2 0 98 100

[A35] Other Fish (other Pisces)Mangrove Killifish 5 0 34 39

[A35] Other Fish (other Pisces)Sheepshead Minnow 1 0 0 1

[A35] Other Fish (other Pisces)Xiphophrus hellerii 27 0 0 27

[A35] Other Fish (other Pisces)Heterandria formosa 10 0 0 10

[A35] Other Fish (other Pisces)Limia nigrofasciata 9 0 0 9

[A35] Other Fish (other Pisces)Xenoteca eiseni 1 0 0 1

[A35] Other Fish (other Pisces)Gobius paganellus 1 0 0 1






[A29_2] Other birds (other Aves)Taeniopygia guttata 5 0 0 5


[A35] Other Fish (other Pisces)Three spined stickleback 2537 0 0 2537








[A35] Other Fish (other Pisces)Carp 45 0 0 45


































[A35] Other Fish (other Pisces)stickleback 844 0 0 844







[A35] Other Fish (other Pisces)CARP 478 0 0 478

[A35] Other Fish (other Pisces)TROUT 2408 0 0 2408

[A35] Other Fish (other Pisces)LUMPSUCKER 104 0 0 104






















[A7] Other Rodents (other Rodentia)Meriones lybicus 37 0 0 37

[A7] Other Rodents (other Rodentia)Meriones shawii 5 0 0 5




[A7] Other Rodents (other Rodentia)Voles 31 0 0 31















































[A35] Other Fish (other Pisces) 0 0 133 133

AT_D-3.2.pdf

D - 3.2 Provide representative information on approximate numbers, species, types of methods and their

related severities of tissue sampling for the purposes of genetic characterisation carried out with and

without project authorisation.

Mandatory question to those Member States that use, breed and/or supply genetically altered

animals- please complete the requested information below

If the number of lines is not sufficient, please upload a separate document for any remaining entries just below.

1. Name of species

2. Number of animals

3. Used tissue sampling

method

4. Mild

5. Moderate

6. Severe

7. Non-invasive /

without a project

authorisation

8. Surplus material from

identification

1 Mus musculus 100050 distal phalanx biopsy x

2 Mus musculus 52364 Ear punch x

3 Mus musculus 40123 Tail tip biopsy x

4 Danio rerio 2812 Fin clip x

5 Mus musculus 1038 Blood x

6 Rattus norvegicus 891 Tail tip biopsy x

7 Rattus norvegicus 625 Ear punch x

8 Mus musculus 372 Visualization under blue/green light

x

9 Oryzias latipes 316 Fin clip x

10 Danio rerio 149 Fin clip x

11 Mus musculus 69 Visual judging of incisors

x

12 Mus musculus 38 Visualization under laser light

x

13 Mus musculus 10 Hair bulbs x

14 Oryctolagus cuniculus 6 Blood x

FR_D-3.2.pdf

1. Name of

species

2. Number

of animals

3. Used tissue

sampling method

4.

Mild

5.

Moderate

6.

Severe

7. Non-

invasive /

without a

project

8. Surplus material from

identification

1 [A1] Mice (Mus musculus) 58.706 Biopsie auriculaire x oui (ADN)

[A1] Mice (Mus musculus) 240.623 Biopsie caudale x oui (ADN)

[A1] Mice (Mus musculus) 274 Biopsie de peau (poinçonnage) x oui (ADN)

[A1] Mice (Mus musculus) 149 Biopsie follicule pileux (poils) x non

[A1] Mice (Mus musculus) 362 Biopsie sanguine x oui (ADN)

[A1] Mice (Mus musculus) 106.242 Phalangectomie x x x x oui (ADN)

[A1] Mice (Mus musculus) 25.840 Prélèvement post-mortem x oui (ADN)

TOTAL 432.196

2 [A2] Rats (Rattus norvegicus) 3.106 Biopsie auriculaire x oui (ADN)

[A2] Rats (Rattus norvegicus) 1.721 Biospie caudale x oui (ADN)

[A2] Rats (Rattus norvegicus) 160 Phalangectomie x oui (ADN)

[A2] Rats (Rattus norvegicus) 2.182 Prélèvement post-mortem x oui (ADN)

TOTAL 7.169

3 [A8] Rabbits (Oryctolagus cuniculus) 133 Biopsie auriculaire x oui (ADN)

TOTAL 133

4 [A32] Xenopus (Xenopus laevis and Xenopus tropicalis) 88 Prélèvement post mortem x oui (ADN)

TOTAL 88

5 [A34] Zebra fish (Danio rerio) 1.406 Biopsie de nageoire caudale x oui (ADN)

TOTAL 1.406

Mandatory question to those Member States that use, breed and/or supply genetically altered animals : Provide representative information on approximate numbers, species, types of methods and their

related severities of tissue sampling

1/1

IT_D-3.2.pdf

1. Specie 2. Numero animali 3. Metodo di prelievo tessutale 4. Lieve 5. Moderata 6. Grave7. Non cruento/senza

autorizzazione progetto8. Materiale di scarto dall’identificazione

Topi (Mus musculus) 1767

genotipizzazione mediante prelievo della parte

terminale della coda (<5mm) 1767 0 0 0


Genotipizzazione mediante prelievo della parte

terminale della coda ( 263 0 0 0


Genotipizzazione mediante prelievo della parte

terminale della coda ( 62 0 0 0

Topi (Mus musculus) 593 taglio coda ( 593 0 0 0

Topi (Mus musculus) 258 0 0 0 258

Raccolta materiale deciduo da numerazione

auricolare

Topi (Mus musculus) 400 biopsia della coda 400 0 0 0

Topi (Mus musculus) 1055 taglio coda 1055 0 0 0

Topi (Mus musculus) 428 Lampada UV 428 0 0 0

Topi (Mus musculus) 399 Prelievo coda con anestesia locale 0 399 0 0

Topi (Mus musculus) 118 Biopsia orecchio 118 0 0 0 Biopsia orecchio

Topi (Mus musculus) 2919 biopsia caudale 2919 0 0 0

Topi (Mus musculus) 1825 prelievo ematico 1825 0 0 0

Topi (Mus musculus) 2554 biopsia auricolare 2554 0 0 0 biopsia auricolare

Topi (Mus musculus) 600 Taglio coda 3-5mm 600 0 0 0


Topi (Mus musculus) 1158 prelievo ematico 1158 0 0 0



Taglio dell'estremità della coda al momento dello

svezzamento previa anestesia generale ottenuta

mediante inalazione di isoflurano alla

concentazione alveolare di 1,5%(induzione 4%)

per il minor tempo possibile 0 253 0 0

Topi (Mus musculus) 1121 Prelievo di tessuto epidermico 1121 0 0 0

Topi (Mus musculus) 212 prelievo bioptico caudale ¿3 mm 212 0 0 0

Topi (Mus musculus) 16 prelievo pellet fecali 0 0 0 16

Topi (Mus musculus) 528 campione bioptico 2mm punta della coda 0 0 0 528

Ratti (Rattus norvegicus) 409 Prelievo tessuto 409 0 0 0

Topi (Mus musculus) 896 tail, ear biopsy 896 0 0 0

Topi (Mus musculus) 1200 Taglio delle dita o della coda a P7-P10 0 1200 0 0

Topi (Mus musculus) 102 taglio estremità coda effettuato una sola volta 102 0 0 0

Topi (Mus musculus) 3000 taglio coda < 3 mm 3000 0 0 0

Ratti (Rattus norvegicus) 100 taglio coda < 3 mm 100 0 0 0

Topi (Mus musculus) 300 tail <= 3mm 300 0 0 0

Ratti (Rattus norvegicus) 39 tail <= 3mm 39 0 0 0


Taglio apice coda, meno di 3mm, entro 8gg dalla

nascita 59 0 0 0


Taglio apice della coda, meno di 1mm, emtro il 21

gg dalla nascita 15 0 0 0

Topi (Mus musculus) 20 biopsia auricolare 20 0 0 0

Topi (Mus musculus) 158 prelievo tessuto auricolare 158 0 0 0


Topi (Mus musculus) 103 Analisi PCR su DNA di biopsie caudali 0 0 0 103

Topi (Mus musculus) 49 Biopsia Orecchio 0 0 0 49


taglio delle falangi dopo anestesia locale entro i

10 giorni di vita 0 773 0 0

taglio delle falangi dopo anestesia locale entro i

10 giorni di vita

Topi (Mus musculus) 1422 Taglio dell'estremità della coda <3mm 1422 0 0 0


Biopsia dell'orecchio. Acquistato "ear punch"

diametro inferiore di 2mm nel gennaio 2018 come

refinement del nostro stabilimento di utilizzo 579 0 0 0

Topi (Mus musculus) 5920 prelievo tessuto coda 5920 0 0 0

Topi (Mus musculus) 515 prelievo tessuto coda 0 515 0 0

Topi (Mus musculus) 137 pellet fecale 0 0 0 137

Topi (Mus musculus) 417 biopsia caudale < 3mm 0 417 0 0

Topi (Mus musculus) 818 Prelievo di tessuto caudale 0 818 0 0


Biopsia caudale (lieve). La maggioranza degli

animali viene genotipizzata con il materiale di

scarto (biopsia auricolare) dell'identificazione. 76 0 0 0

Topi (Mus musculus) 2599 taglio coda a 24 ore o nei primi 15 giorni di vita 2599 0 0 0

Topi (Mus musculus) 651 Taglio della coda 0 651 0 0

Topi (Mus musculus) 63 Ear punch 63 0 0 0 Ear punch

Suini (Sus scrofa domesticus) 194 Caudectomia parziale 194 0 0 0


biopsia caudale con taglio di lunghezza inferiore a

3mm entro 21 giorni dalla nascita con

applicazione di crema anestetica locale 100 0 0 0


il meteriale di scarto proveniente dalle

identificazioni tramite ear punch del diametro

inferiore a 2 mm viene utilizzato per la

genotipizzazione

Topi (Mus musculus) 288 taglio coda per genotyping 288 0 0 0

Topi (Mus musculus) 2908 Biopsia caudale 2908 0 0 0


utilizzato esclusivamente per tutte le

genotipizzazioni

Topi (Mus musculus) 1101 Taglio falangi 0 0 1101 0

Topi (Mus musculus) 492 Prelievo frammento coda 492 0 0 0

Topi (Mus musculus) 71 Taglio di una parte di coda 71 0 0 0

Topi (Mus musculus) 140 0 0 0 140 marcatura auricolare

Topi (Mus musculus) 30 taglio porzione distale della coda 30 0 0 0

Topi (Mus musculus) 390 prelievo venoso plesso retrorbitale 0 390 0 0


Topi (Mus musculus) 45 prelievo cutaneo 45 0 0 0

Topi (Mus musculus) 50 prelievo cutaneo 50 0 0 0


Topi (Mus musculus) 253 tail biopsy 253 0 0 0


prelievo di tessuto al fine della genotipizzazione:

taglio coda, 547 0 0 0


Biopsia dell'orecchio mediante ear punch

(diametro < 2mm.) associato al tag. In futuro

identificheremo tutti gli animali o mediante ear

punch oppure attraverso tag più prelievo di un

bulbo pilifero per estrarre il DNA. 3210 0 0 0

Pesci zebra (Danio rerio) 700

fin clipping oppure mediante riconoscimento

visivo allo stadio larvale Considerando che la

porzione di cosa amputata ricresce nell'arco di

poche settimane al momento non abbiamo

sistemi alternativi migliori 700 0 0 0

Topi (Mus musculus) 110 punch auricolare 110 0 0 0


Biopsia caudale a 3 settimane di eta' Viene

prelevata una porzione molto piccola (pochi mm)

dell'estremita' della coda ad un eta' alla quale le

vertebre coccigee non sono ancora ossificate.

Questo metodo e' in fase di progressiva

sostituzione l'utilizzo di materiale di scarto di

pratiche di identificazione (foro nel padiglione

auricolare - ear punching) 4113 0 0 0


Per i 6897 topi indicati come "non cruento" la

getopizzazione e' stata effettuata su materiale di

scarto di pratiche di identificazione (foro nel

padiglione auricolare - ear punching).


Topi (Mus musculus) 150 PCR su DNA di biopsie caudali 150 0 0 0

Topi (Mus musculus) 12 biopsia della coda 12 0 0 0


biopsia di un piccolissimo frammento di tessuto

previa applicazione di anestetico locale,

proveniente da pratiche di identificazione 168 0 0 0

Pesci zebra (Danio rerio) 164

Fin clipping. E' un metodo abbastanza affinato

poichè la pinna si rigenera nel corso delle

settimane successive 164 0 0 0


taglio della coda in animali p7-p9 di età inferiore

ai 14 giorni 4577 0 0 0


prelievo 3 mm tessuto caudale o padiglione

auricolare sotto anestesia (Refinement:

prevediamo l'introduzione di prelievo materiale

bioptico tramite ear puncher con diametro 713 0 0 0

Topi (Mus musculus) 246 prelievo di frammento di coda minore di 5 mm 246 0 0 0

Topi (Mus musculus) 52 identificazione mediante lampada UV 0 0 0 52



taglio della falange entro 2 mm di lunghezza e 12

giorni dalla nascita con applicazione di pomata

anestetica 0 111 0 0


Il metodo utilizzato ai fini dell'esecuzione della

tecnica di genotipizzazione consiste nel prelievo ,

al 14 esimo giorno di vita, di una porzione

terminale della coda, di lunghezza minore o

uguale a 3 mm, post apposizione di una pomata

anestetica (luan 2.5%). Tale tecnica presenta un

livello di sofferenza classificato come lieve. 2340 0 0 0


biopsia caudale con taglio di lunghezza inferiore a

3mm entro 21 giorni dalla nascita con

applicazione di crema anestetica locale 70 0 0 0


il meteriale di scarto proveniente dalle

identificazioni tramite ear punch del diametro

inferiore a 2 mm viene utilizzato per la

genotipizzazione


taglio della falange entro 2 mm di lunghezza e 12

giorni dalla nascita con applicazione di pomata

anestetica 0 133 0 0


Marcatura auricolare effettuata con Anestesia

gassosa a base di isoflurano e ossigeno. 318 0 0 0

Topi (Mus musculus) 1752 genotipizzazione dopo taglio della coda 1752 0 0 0


Prelievo coda (Prelievo cutaneo dell'apice della

coda) 62 0 0 0


PCR su frammento di coda. Il frammento viene

prelevato prima delle 3 settimane di età secondo

Linee Guida 652 0 0 0


PCR su frammento di orecchio. Il frammento

viene ottenuto durante la marcatura per

l¿identificazione dell¿animale 174 0 0 0


Estrazione DNA da tessuto del padiglione

auricolare tramite pinze per taglio idonee 2301 0 0 0


Taglio punta coda, in seguito di anestesia a

frigore. 615 0 0 0


prelievo di tessuto dall'orecchio (ear punch): non

prevede altro metodo di marcatura e permette

l'identificazione dell'individuo oltre alla

genotipizzazione dello stesso 38 0 0 0

Altri anfibi (altri Amphibia) 4

Bagno di immersione con 1% tricaina

metansolfonato (MS-222). 0 4 0 0

Topi (Mus musculus) 291 taglio dell'apice della coda 0 291 0 0


taglio della terza falange dopo anestesia locale

con lidocaina topica al giorno P7 0 32 0 0


Asportazione di un piccolo frammento della coda

(<3mm) 1282 0 0 0

SE_D-3.2.pdf

SWEDEN

ADDITIONAL DATA THAT COULD NOT BE ENTERED IN THE TABLE IN THE EU SURVEY

1. name of species 2. number of animals 3. used tissue sampling method 4. Mild 5. moderate 6. severe 7. non-invasive/without a project authorisation 8. surplus material fråm identification

mouse (mus musculus) 5336 tail clipping x

mouse (mus musculus) 18134 toe clipping x

mouse (mus musculus) 1544 ear clipping x

mouse (mus musculus) 178212 ear clipping x

UK_D-3.2.pdf

Animal Species Specify other Number of

animals

Re-sampling for genotyping Genotyping method

used

Specify other Severity Invasive Surplus

material from

identification

[A12] Other carnivores (other Carnivora) Badger 17 [G1] Not previously sampled for genotyping [M4] Hair/fur sample

Sub-

threshold [N] No [N] No

[A1] Mice (Mus musculus) 40 [G1] Not previously sampled for genotyping [M2] Ear notching [SV2] Mild [Y] Yes [N] No



[A1] Mice (Mus musculus) 103 [G2] Previously sampled for genotyping [M2] Ear notching [SV2] Mild [Y] Yes [N] No

[A1] Mice (Mus musculus) 54 [G1] Not previously sampled for genotyping [M10] Blood sample [SV2] Mild [Y] Yes [N] No

[A1] Mice (Mus musculus) 33 [G2] Previously sampled for genotyping [M10] Blood sample [SV2] Mild [Y] Yes [N] No

[A1] Mice (Mus musculus) 231 [G1] Not previously sampled for genotyping [M2] Ear notching

Not

applicable

Not

applicable [Y] Yes


Not

applicable [N] No [Y] Yes

[A1] Mice (Mus musculus) 7 [G1] Not previously sampled for genotyping [M1] Tail tip [SV2] Mild [Y] Yes [N] No

[A1] Mice (Mus musculus) 98 [G2] Previously sampled for genotyping [M2] Ear notching [SV2] Mild [N] No [N] No


Not



Not

applicable [Y] Yes [Y] Yes





[A1] Mice (Mus musculus) 147 [G2] Previously sampled for genotyping [M1] Tail tip [SV2] Mild [Y] Yes [N] No

[A2] Rats (Rattus norvegicus) 138 [G1] Not previously sampled for genotyping [M2] Ear notching

Not



[A1] Mice (Mus musculus) 12350

[G3] Do not know if previously sampled for

genotyping [M2] Ear notching

Sub-

threshold [Y] Yes [N] No


[A2] Rats (Rattus norvegicus) 386 [G1] Not previously sampled for genotyping [M1] Tail tip [SV2] Mild [Y] Yes [N] No

[A2] Rats (Rattus norvegicus) 369



Sub-




Not



Not



Not



Not



Not


[A1] Mice (Mus musculus) 1571 [G2] Previously sampled for genotyping [M2] Ear notching

Not



Not



Not




Not

applicable

Not

applicable [Y] Yes



Not

applicable

Not

applicable [Y] Yes



[A1] Mice (Mus musculus) 80 [G1] Not previously sampled for genotyping [M8] Toe removal [SV2] Mild [Y] Yes [N] No





[A2] Rats (Rattus norvegicus) 45 [G2] Previously sampled for genotyping [M10] Blood sample [SV2] Mild [Y] Yes [N] No

[A2] Rats (Rattus norvegicus) 44 [G1] Not previously sampled for genotyping [M2] Ear notching [SV2] Mild [Y] Yes [N] No


Not

applicable

Not

applicable [Y] Yes

[A34] Zebra fish (Danio rerio) 8 [G1] Not previously sampled for genotyping [M6] Fin clip [SV2] Mild [Y] Yes [N] No




Sub-



Not

applicable

Not

applicable [Y] Yes





Not

applicable

Not

applicable [Y] Yes



Not

applicable

Not

applicable [Y] Yes


[A1] Mice (Mus musculus) 25 [G1] Not previously sampled for genotyping [M1] Tail tip [SV2] Mild [N] No [N] No

[A1] Mice (Mus musculus) 38 [G2] Previously sampled for genotyping [M2] Ear notching [SV2] Mild [N] No [N] No


Not


[A34] Zebra fish (Danio rerio) 4568 [G1] Not previously sampled for genotyping [M6] Fin clip [SV2] Mild [N] No [N] No


Not




Not

applicable

Not

applicable [Y] Yes




Not



Sub-


[A1] Mice (Mus musculus) 727 [G1] Not previously sampled for genotyping [M2] Ear notching [SV2] Mild [N] No [N] No


Not




Not

applicable

Not

applicable [Y] Yes


Not

applicable

Not

applicable [Y] Yes


Not

applicable

Not

applicable [Y] Yes


Not

applicable

Not

applicable [Y] Yes


Sub-



Sub-



[A17] Cattle (Bos primigenius) 86 [G1] Not previously sampled for genotyping [M10] Blood sample [SV2] Mild [Y] Yes [N] No


Not


[A1] Mice (Mus musculus) 402 [G1] Not previously sampled for genotyping [M9] Other

Detection of fluorescent

gene markers using

Nightsea ™ goggles

Sub-




[A35] Other Fish (other Pisces) Medaka 51 [G1] Not previously sampled for genotyping [M6] Fin clip [SV2] Mild [Y] Yes [N] No

[A17] Cattle (Bos primigenius) 42 [G1] Not previously sampled for genotyping [M10] Blood sample [SV2] Mild [Y] Yes [N] No





Not




[A1] Mice (Mus musculus) 56 [G1] Not previously sampled for genotyping [M8] Toe removal

Not



[A34] Zebra fish (Danio rerio) 82 [G1] Not previously sampled for genotyping [M7] Mucus sample

Sub-


[A34] Zebra fish (Danio rerio) 479 [G1] Not previously sampled for genotyping [M9] Other

fluorescence screen of

embryos

Sub-


[A34] Zebra fish (Danio rerio) 70 [G1] Not previously sampled for genotyping [M9] Other

intercross and embryo

genotyping

Sub-


[A27] Other Mammals (other Mammalia)

Laboratory

opossum 15 [G1] Not previously sampled for genotyping [M2] Ear notching [SV2] Mild [Y] Yes [N] No



Not

applicable

Not

applicable [Y] Yes


Not



Not


[A28] Domestic fowl (Gallus gallus domesticus) 103 [G1] Not previously sampled for genotyping [M10] Blood sample [SV2] Mild [Y] Yes [N] No

[A28] Domestic fowl (Gallus gallus domesticus) 1873 [G1] Not previously sampled for genotyping [M9] Other Chorioallentoic membrane

Sub-


[A28] Domestic fowl (Gallus gallus domesticus) 155 [G2] Previously sampled for genotyping [M10] Blood sample [SV2] Mild [Y] Yes [N] No




Not




genotyping [M1] Tail tip [SV2] Mild [N] No [N] No




Not


[A34] Zebra fish (Danio rerio) 1771


genotyping [M6] Fin clip [SV2] Mild [N] No [N] No


Not

applicable

Not

applicable [Y] Yes



[A14] Pigs (Sus scrofa domesticus) 6 [G1] Not previously sampled for genotyping [M3] Saliva

Sub-


[A10_1] Beagles (Canis lupus familiaris) 24 [G1] Not previously sampled for genotyping [M3] Saliva

Sub-


[A10_1] Beagles (Canis lupus familiaris) 11 [G2] Previously sampled for genotyping [M10] Blood sample [SV2] Mild [Y] Yes [N] No




Not






Not

applicable

Not

applicable [Y] Yes

[A32] Xenopus (Xenopus laevis and Xenopus

tropicalis) 25 [G1] Not previously sampled for genotyping [M8] Toe removal [SV2] Mild [Y] Yes [N] No


[A32] Xenopus (Xenopus laevis and Xenopus

tropicalis) 160 [G1] Not previously sampled for genotyping [M1] Tail tip [SV2] Mild [Y] Yes [N] No





Not


[A1] Mice (Mus musculus) 95 [G1] Not previously sampled for genotyping [M10] Blood sample [SV2] Mild [N] No [N] No


Not



Not




Not

applicable

Not

applicable [Y] Yes


Not

applicable

Not

applicable [Y] Yes










Not

applicable

Not

applicable [Y] Yes






Not

applicable

Not

applicable [Y] Yes



[A1] Mice (Mus musculus) 95 [G1] Not previously sampled for genotyping [M8] Toe removal

Not

applicable

Not

applicable [Y] Yes

[A1] Mice (Mus musculus) 44 [G1] Not previously sampled for genotyping [M9] Other Fluorescence

Sub-


[A1] Mice (Mus musculus) 27 [G1] Not previously sampled for genotyping [M1] Tail tip

Not

applicable

Not

applicable [Y] Yes



Not

applicable

Not

applicable [Y] Yes





[A35] Other Fish (other Pisces)

Astyanax

mexicanus 4 [G1] Not previously sampled for genotyping [M6] Fin clip [SV2] Mild [Y] Yes [N] No


Not

applicable

Not

applicable [Y] Yes





Not




genotyping [M1] Tail tip [SV2] Mild [Y] Yes [N] No



genotyping [M1] Tail tip

Not




genotyping [M9] Other GFP goggles

Sub-




genotyping [M7] Mucus sample [SV2] Mild [N] No [N] No

[A35] Other Fish (other Pisces) Medaka 186


genotyping [M7] Mucus sample [SV2] Mild [N] No [N] No


Not




genotyping [M2] Ear notching [SV2] Mild [Y] Yes [N] No



Not





Not







genotyping [M2] Ear notching [SV2] Mild [N] No [N] No







Not





Not




genotyping [M10] Blood sample [SV2] Mild [N] No [N] No




Not





Not











Not





Sub-





Not






Not





Not





[A19] Marmoset and tamarins (eg. Callithrix

jacchus) 37


genotyping [M4] Hair/fur sample

Sub-








Not








Not





Not











Not





Not








Not





Not








Not








Not





Not





Not




genotyping [M6] Fin clip [SV2] Mild [N] No [N] No



genotyping [M6] Fin clip [SV2] Mild [Y] Yes [N] No




Not








Not





Not





Not





Not





Not







genotyping [M6] Fin clip [SV2] Mild [Y] Yes [N] No




Not





Not





Sub-





Sub-





Not








Not





Not








Not





Not








Not








Not








Not








Not



Not

applicable

Not

applicable [Y] Yes


Not



[A1] Mice (Mus musculus) 15 [G2] Previously sampled for genotyping [M4] Hair/fur sample [SV2] Mild [N] No [N] No


Not



Not



Not


[A34] Zebra fish (Danio rerio) 40 [G1] Not previously sampled for genotyping [M6] Fin clip [SV2] Mild [N] No [N] No

[A34] Z

explanatory words · web viewannex – part ii member state supplementary submissions under article...

Documents