Extraction of Nicotine By: DJ Hall and Brittany Hicks

PurposeDetermine if nicotine is extractable by means of

supercritical fluidRun more than one sample type to determine if

this makes a significant difference

SFESupercritical Fluid Extraction

Uses high pressure CO2

Used to extract organics from different types of matrixes

Allows for easy separation

ProblemsAll soluble organics will be separated form matrix

GC-MSGas Chromatography Mass Spectroscopy

Separation of violate organicsDetermine the analyte separated at specific

retention times using MS

Nicotine (162.12g/mol)Poison (parasympathomimetic alkaloid)Formed in nightshade family of plantsAntiherbivor chemicalSmall doses

StimulantRelaxation

Large dosesFatal

Extraction2 Cigarettes Marlboro blend 272 Pieces Nicorette Gum 2mg50 Drops of e-cigarette liquid 2.4% by weight

Extraction cont.All extractions were obtained by bubbling into

methanol and using SFE methanol modifier pumpCigarettes

Ground using mortar and pestle

Nicorette GumCut into small pieces

E-cigarette liquidCelite as inert matrix

Determination of NicotineAll samples were diluted to 50ml of methanolA standard was created by diluting 4mg of

Nicotine in 50ml of methanolStandards and Samples were run using the GC-MS

Reference Mass Spectra

Standard

Nicotine peak

Cigarettes

Nicotine peak

Cigarettes cont.

E-Cigarette Liquid

Nicotine peak

E-Cigarette Liquid Cont.

Nicorette Gum

Chromatogram ComparisonE-Cigarette Liquid

Nicorette Gum

Cigarette

Standard

ConclusionIt is possible to extract nicotine using the SFEBoth the E-Cigarette and Cigarette samples

contained nicotineThe Nicorette Gum showed undetectable amounts

of nicotineNicotine may have be contained in the gum residue

that was filtered before dilution

Future ProjectQuantify the amounts of nicotine extractedDetermine the extraction efficiency

Work CitedFischer, M. and Jefferies, T. Optimization of

Nicotine Extraction from Tobacco Using Supercritical Fluid Technology with Dynamic Extraction Modeling. J.Agric. Food Chem. (1996), 44, 1258-1264.