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HEMCITNDRACHARYA NORTH GUJARAT UNIVERSITYPA7]AN - 384265

NAAC Accreditation Grade -

FACULTY OF SCIENCE

B. Sc. - BIOTECIINOLOGYNew Syllabus and Exam Scheme

Semester= f TO IIWith Semester / CBCS / Grading pattern

W.E.F.June- 2011.

t>

^'(/v/Date:30-06-2011 TOTAL PAGE. 13

C

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HemchandracharvaNorth Gujarat Universitv

PATAN _ 384 265

Syllabus According To CBCS Semester pattern

B. Sc. (Biotechnology) Syllabus

(Semester I & ID(With Effect From June 20tl)

$}c. (Biotecnnouey)

B . S". (Biotechnology)

(With effect from June 2011)

Semester ICore Compulsory Course (CCC)CBT l-I Introduction to Biotechnology and Cell biology

Elective Course (EC) for Biotechnolog5,

EBT I Biological evolution

PBT - I Intsdisciprinafy rereva*e and Advancement of Biotechnorogy Semester Ir

Practical core course (pCC)

Semester II

Core Compr,ltqry Course (CCC)CBT l-II Molecules of life

Elective Course (EC) for BiotechnologrEBT I I BiodiversitvEBT 1t Biocomputing

Practical core course (pCC)

lhmdrandracharya ilo.ilt Guiarat University, pATAN gS4 Gz

t5N.Sc. ( Biotechnologl,)

PAPER STYLECore Course Paper (Biotechnology)

70 marks question paper

Q.1 MCQ type questions (one mark each ) 10 marks(In Q.1 there must be minimum two questions from each unit)

Q.2 Give very brief answers ( Two marks each) 10 marks(ln Q.2 there must be minimum two questions from each unit)

OR

Q.2 Give very brief answers( Two marks each) 10 marks

Q.3 Short note type question (Attend any four out of eight) 32 marks(In Q.3 there must be minimum two questions from each unit)

Q.4 Detailed note type question (Attend any two out of four) 18 marks

(In Q.4 there must be one question from each unit)

PAPER STYLE' Elective Course Paper (Biotechnology)

50 marks question PaPer

Q.l MCQ type questions (one mark each ) 10 marks

(In Q.1 there must be minimum five questions from each unit)

Q.2 Short note type question (Attend any four out of eight) 24 marks

(In Q.2 there must be minimum four questions from each unit)

Q.3 Detailed note type question (Attend any two out of four) l6marks

(In Q.3 there must be minimum two questions from each unit)I

4tlemchandracharya North Guiarat University' PATAN 384 265

(&. sc. 6rot"rnrotogl, I

Course pattern Subject :Bioteclrnology

Semester IB.Sc

Semester Paper Instruction(

hr per u'eck)

Marks Credits

Internal External total

Core Course compulsory(CCC)

I Core course

ccc-r-r

4 30 70 t00 4

Core course 2

ccc-Il-r4 30 70 r00 4

Core course 3

ccc-[r-1

4 30 7o t00 4

Practical core course (PCC)

Practical core course

(For biotechnology)

PCC-I.1

4 50 50 2

Practical core course2-

PCC-[-1

4 50 50 2

Practical core

PCC-UI-1

50 50 2

Foundation Course (FC)

.50Foundation | 2(Compulsory) course

I

(Generic) - English I(L.L.) |FCG-I I

t5 35

Elective Course (EC)

Elective | 2(Generic) Course -I

I

ECG-I I

50 50 2

Elective (Subjecr)

Course -IECS-1

2 50 .50 2

30 llr5 495 6{10 24

Hemchandracharya North Guiarat University, PATAN g84 265

ll'

M. S r: - ( B iot ec hno Log1, )

Semester 2(First year B.Sc)

Semester Paper Instruction(

hr per l'eek)

Marks Credits

Internal External total

Core Course compulsory'(CCC)

2 Core course

ccc-Ir

4 30 70 r00 4

Core course 2

CCC.II-II

4 30 70 r00 4

Core course 3

CCC.TI.II

4 30 70 t00 4

Practical core course (PCC)

Practical core course

(For biotechnology)

PCC-r-1I

4 50 50 2

Practical core coursez-

PCC-II-1I

4 50 .50 2

Practical core

PCC-UI-1I

4 50 50 2

Foundation Course (FC)

Foundation(Conpulsory) course

(ceneric) - English(L.1.)FCG.1I

2 I5 35 50

Elective Course (EC)

Elective(Ceneric) Course -IECG.lI

2 50 -50 2

Elective (Subjec0

Course -IECS-1I

2 50 50 2

30 105 495 600 u

Hemchandracharya North Guiarat University, PATAN 384 265

^An. r\ r"fr.5c. (Biotechnobgyl

a

B.Sc Biotechnology

SEMESTER I

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lhmohandtachayr lbrtr cuia.at University, plmfl aSa mi6

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vM-Sc. (Biotechrology)

SMESTER 1

Core Course Compulsory (CCC l-l)

cBT t-l

Inkoduction to Biotechnology and Cell Biology

Unit 1

1.1. Introduction to Biotechnology

1.2. Domains of Biotechnology

1.3. Applications of Biotechnology.: Agriculture ,Pharmaceutical, Environment, Fermentation

1.4, State, nationaland international level commercial opportunilies in Biotechnology sector.

Unit 2

2.1. Microscopy: Fundamental of microscope, light microscopy and specimen preparation Bright field

microscopy, Dark field mictoscopy.

2.2. Morphology of Bacterial cell: Size, shape and arrangement of bacterial cells ,External structure:

Flagella, Pili, Fimbriae, Prosthacate

2.3. Boundary layen Capsule, cell wall , cell membrane

2.4. Dormant forms: Spores and cyst

Unit 3

3.1. General organization of eukaryotic cell External stfuctures: Flagella, cilia The cell envelope: boundary

- layer: cell wall, cell membrane

3.2. Intelnal structures: Cytoplasm, cytoskeleton, nucleus and nucleolus

3.3. Endoplasmic Reticulum, Golgi apparatus, Mitochondria Lysosome, Micro bodies (Glyoxysome and

Peroxisome) ChloroPlast,.

3'4. chromosome: Size, shape, types and basic structure of chromosome, euchromatin and

heterochromatin Gianl chromosome: Polytene chromosome and lamp brush chromosome

Unit 4. . 4.1. Cell cycle and overuiew of its regulation.

4.2, Mitosis and meiosis

4.3. Cell -Cell interaction

4.4. Endocytosis and exocytosis

D

Hemchandracharya North Guiarat University, PATAN 384 265

W-Sc- 1Biot"rt notogyl

Elective Course (EC)

EBT 1

Biological evolution

Unit 1

1.1. Theories of evolution: Charles Daruin, Lamark and Wdlace

1.2. Ch€mical and biological evoluiion.,

1.3. Five kingdom classification system.

1.4. Understanding Species: Concept of Species and Speciation, Morphological and Biological explanation

for species, Types of Speciation, Rates of Speciation

Unit 2

2.1. lsolation: Concept of lsolation, Mechanism of lsolation,

lsolation.

2.2. Reproductive isolation, Types of Reproductive isolation,

formation.

2.3. Adaptation: Concept of Adaptation, Types ol Adaptation

2.4. Adaptation and predators, adaptation and population.

Factor responsible for isolation, Types of

Role of Reproductive isolation in species

Elective Course (EC): EBT1

Interdisciplinary relevance and Advancement of BiotechnologyUnit-1

1.1 What is interdisciplinary areas?

1.2 Biotechnology and relevance with Chemistry, Physics and Maths1.3 Biotechnology and relevance with Agriculture,Medical,pharmaceuticals1.4 Advantage of Interdisciplinary subject

Unil-2

2.1' Advancement of Biotechnology in crop lmprovement for edible vaccine and biopestiside.2.2. Advancement of Biotechnology in Fermentation for organic acids2.3. Advancement of Biotechnology in Health care lor vacci2.4. Advancement of Biotechnology in Sustainable development for

Environment

l'^

Hemchandracharya North cuiarat University, pATAN 3S4 265

.n(\$.5c. ( Biotechnotogl. )

Semesler I

Practlcal Core courso (PCCFI)

1. Introduction to lab environment-satety measures and introduction to lab equipments, glass wares and

accessories ,Disposal of laboratory waste and cuftures

2. Microscopy : Simple, compound and phase contrdst; Basic components of microscope and their

wofting principle

3. Staining techniques :Simple-Monochrome and Negalive Diffsrential- Grams and SpecialCapsule,

Spore, Cell wall.

4. Study of Bactedal Motility

5. Micromstry: Measurement of given biological sample

6. Use of Heamocytometer and determination of cell densities of Yeast cell

7. Preparation ol pemanent slides showing different stages of cell division - Meiosis and Mitosis

llemdrandracharya ll,orth Guiarat Univercily, PATAN 384 265I

0-,

&.5c. Giotechrclosyl

B.Sc Biotechnology

SEMESTER II

Hemchandr.duye tlor0r Guiant tfidvrrsity, PATAN gS4 265

7C,

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F.Sc. ( Biotechnology)

SRMESTER II

Core Compulsory course (CCC l-ll)

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Molecules of life

Unit 1

1.1. Overview of major elemenls involved in formation of biomolecules: C,N,P,S,O,H Water: chemical

composition, role ol hydrogen bonds, interactions with polar and non polar molecules, Water as

reactivate, ionization of water, Solvenl properties of water and importance

1.2. Buffers: Buffer systems and buffer system of blood, weak acid and weak base, dissociation constant of

weak acid and base ,

1.3, pka values and their importance, pH and pH scale, acid dissociation constant pka and tilration curve,

Handerson-Hasselbalch eouation

1'4.structureofatomsandmo|ecu|esandchemica|bondS(coVa|ent'ionic,Hydrogen,vanderwaa|'s,hydrophobic).

Unit 2

2.1. Carbohydrates Monosaccharides: Nomenclature and Classification, Hawarth and fischer projection.

2.2. Monosaccharide as reducing agent, slereoisomerism

2.3. Disaccharides formation and its biological rmportance.

2.4. Poly saccharide : types and biological importance

Unit 3

3.1. Amino acid: Classilication and properties.

3.2, Proteins: Primary and secondary structure of proteins, tertiary and quaternary structure of proteins

3.3. Vitamins: water soluble and fat soluble vitamins and their biological significance.

3.4. Lipids: Classification, properties and biological importance.

Unit 4

4.1. Nucleotides: structure, chemical properties and functions,

4.2. Structure of DNA double helix

4.3. Altemalive forms of DNA.

4.4. Types, structure and biological functions of RNA.

Hemchandracharya North Guiarat University, PATAN 384 2651L

C

t5fl.5c. (Biotechnotogy)

Elective Course (EC2)

EBT II

Biodiversity

Unit 1

1.1. Definition, Introduction

1'2. Types of biodiversity Genetic Diversity ,species Diversity ,Ecological diversity and functionaldiversity

1.3. overview of microbial diversity

1.4. overview of plant diversity

Unit 2

2,1, lmportance if biodiversity Applications ol internet in societv.2.2. Biodiversity conservation

2.3. Loss of biodiversity.

2.4. Role of biotechnology in biodiversity conseruation.

Elective Course (EC2)

EBT II

Biocomputing

Unit 1

1,1. Introduction to compuler scpnce.1.2. History and generations of Computer.1.3. Basics of Hardware components ol computer.1.4. Basics Software components of computer

Unit 2

2.1. Concepts of internet.

2.2. Applications of internet in societv2.3. Concept of HTML,HTTp,URL,Domain,Search engine2.4. Computer and Internet in Biotechnology

l^

Hemchandracharya North cuiarat University, PATAN 384 265

u

A.

.Sc. (Biorechnology)

Semester ll

Practical Core Course(PCCI'lD

1. PreDaration of shndard solutions and buffer solutions

2. Preparation ol buffer solutions

3. Operation of pH meter and measurement of pH

4. Qualitative lesb lor cabohydrates

5. Qualitative tests for Amino acids

6. Titration cu e ol amino acids and determination of pl, pk1 and pk2

7. Estimalion of reducing sugar.

8. Estimation of non reducing sugar.

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HEMCHNDRACHARYA NORTH GUJARATP A T A N . 384 265

NAAC Accreditation Grade - ', B ''

BIOTECH].{OLOGYSyallbus / Scheme

( In Continuation )

DATE:25-04-2O12

TOTAL PACE.13.

B.Sc. Biotechnology'rremester_ 3 and 4

[Wittr Effect from /une 20LZ)

lnstructionhrs/weed

lnternal€xam

Uni. Exam Total Credit

Semester-IV

Core Compulsory Course (CCC)

ccc-I-5 Core Course-l (Paper-5)

(Biotechnolo$/)3 30 70 .100 3

ccc-r-6 Core Coursel (Paper-6)

(Biotechno logy)3 30 70' 100 3

ccc-Ir-5 €ore Coirrse.ll @gper-5) 3 30 .70. 100. 3

ccc-II-6 Cgre Course- II(Pap-r-B- l 30 7t) 100 3

' PEctieal Core CourSe (pC(l'

)PCC-r-5 Practicai eodCGS-i--

(Paper-5)

(Biotechnoloev)

l

3 50 50 1.5

PCC-I-6 Pracr.lcal Qre Coursi-J(Pbper-6)

(Biotechnology)

3 50 50 1.5

PCC-II-53 50 50 1.5

PCC-rI-63 50 50 1.5

ECG-4 |I

22 15 35 t_j

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Core compulsory Coun;e- I paper 3 .

Paper: Celluiar metabolism.l

Unit-1 Bioenergietics and Thermodynamics

1.

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change,

potential, standard

1. xnzymes: Enzyrne as a .biocataryrt,

."o.ury,nu, cofactor, Nomenclature and^

llassification of enzyme, Basic concept of enzyme substrate reaction.2. Factor affecting on enzyme catalyze reaction3. Overview of catillytic meihanrsms of enzyme:4. Enzyme kinetics: : M-M kinetics , Double reciprocal plot

Unit-3 Enzyme.kinefi cs and regulation

1. Inhibition of gnzyme.

1 3::.:'.l.rrrtrudure of prorein: Hemogiobinr. xegutations ofenzymes_ allosteric and iovalent regulation:4. Basic concept of metabolism.

Unit-2 Basics of EnZyme

Unit:4 Glucose Metabolism

l. flflll.il t"d fate of pvruvate (Arcohor and racic acid rermenration.)

3. Pentose phosphate pathway4. G luco ne ogen esis.

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- B.Sc..Biotechnology

Practical Core Course (PCC-[-3 & pCC-l-4)

[Courrse I (paper 3 and 4))Semester- 3

Practical

Estimation ofprotein & Sugar

l.'

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Assaying Qfvarious enzymes (any threeJ:

5. Amylases.

6. Phosphatases

7. Invertase, ' I8. Protpolyticenzymes.

9. Lipases

10. Effect of Substrate concentration^(Determiniti6n of Km and Vmax).

l], ?:r"*t"e temperarure oprima of rhe enzym12. Effect ofpH on enzyme activity.l3. .Effect of enzyme concentration

Analytical techniques .

. 14. To determine maximum absorption spectra of colored solution.t5. Paper Chrqmatography of Amino acids. ru. rru unromatogrdphy of Amino.acids

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Unit-3 Control of microbial growth

1. Introduction of terms:. Sterilization,Chemotherapy, Antjbiotic etc.

f. Physical agene Mode of acrron.and appFiltration.

3. Chemical agent: Mode of actron and annti

Unit-4 Microbial Diseases and prevention

.,1. ::::vlel on Origin of chemotherapy.

', lJ,lT:,:tt' class of antibiotics based on mode ofaction, Antirungal & Antivirar

3. Introduction of terms: in

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Germicide,

Radiation and

B.Sc. Biotechnology- Elective Course(EC)

Biotechnology(Subjective)

- Semester- 4: introduction of Svstem sner of human

Unit -1 : General

. 1. Respiratory

Pneumonia

Tract. infection':..Rhinitis (common coldl, perti.rssis, Tuberculosis,

. 2. Urogenital Tract infe-ction: Vulvovaginiti, Gonorrhea, Sypirilis

' 3. Infection,bf Digestive Grands and ieritoneum : hepatitis, yeirow fever (liverJ

. 4. Ei,es andears infectlon : Conluncqivitis/scleritis, Otitis mejiaUnit -2 .

'

1' Gastrointestinar rract infections :-shigellosis (dysentery) cholera, Salmonenosis,Amebosis i - '

2. Infections ofNervous System : Meningitis, Tetanus, Botulism

3. Infections ofHematopoietic and Lymphoreticular Sy{tem : plague, HIV infection,Malaria

4. Infections <!f ikin ind Subc.utaneous Cgnnective Tissue : Smallpox, Herpes, Measles

. Elective Course[EC)

. .. . Biotechnology(SubjectiveJ

paper: llant Hormones

Unit -1 Plant hormones_i

1. Definition, General Funcrjohs, Types ofplant Flormones:

2.. Auxins.

3. Gibberellins

+. Lytokinins (= Kinins],

Unit -2 crowth Inhibitors

1. Abscisic Acid

2. Morphactins

3. Oligosaccharins and Other Natural Growth Hormones In plants4. Plant Hormones Versus Animal Hormones

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NOACADEMICCOUNCIL

I Dated : 14/ 05/ 2013,

Resoluation Nos- ]

SUBJECT SYLLABUS&

SCIIEME

SEMESTER

4 5IL 28 Chemistry B. Sc. Sem.- 5 & 6

z 29 Mathematics B. Sc. Sem.- 5 & 6

J 3l Biotechnology B. Sc. Sem.- 5 & 6

A.' )z Zoology B. Sc. Sem.- 5 & 6

5 J.) Botany B. Sc. Sem.- 5 & 6

6 -).+ Micribiology B. Sc. Sem.- 5 & 6

35 Physics B. Sc. Sem.- 5 & 6

8 25 Statestics B. Sc. Sem.- 3 & 4

F:UACADISH\CIRCULAR.doc

HEMCHNDRACEARYA NORTH GAJARAT UNIVERSITY,/ffi\ PATAN_384265

NAAC Accreditation Grade - " B "

FACULTY OF SCIENCE

B. Sc. PROGRA1IIME

BIOTECIINOLOGYSyallbus / Scheme

SEMESTER- V & VI

WITH SEMESTER/CBCS /GRADING PATTERN

W. E. F. JUNE .2013 ( In Continuation )

TOTAL PAGE _ 4.DATE 25-09-20t2

ts*6HEMCHANDRACHARYA NORTH GUJARAT UNIVERSITY' PATAN

B'Sc' Programme with 144 credits

CBCS-semester-Grading Pattern

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(CC) Course

cc-l-7 Core Course-l (PaPer-7) 3 30 '10 100'3

CC-I-8 Crr" C"u.t"-l (PaPer-8) 3. 30 70 100 3

CC-II.9 Core Coursell (PaPer-9) 3 30 70 t00 J

cc-II-10 Core Course-ll (PaPer- l0) 3 30 70 100 3

Practical Core (PC) Course

PC-I.

7,8,9 &

l0

Practical Core Course-l

(Paper-7,8,9 & l0)

t1 200 200 o

Foundation Course (FC)

FC-31Compulsory English (L.L.)

2 t5 35 50 2

Elective Course (E)

EG-31 Elective (Generic) Course 2 50 50 2

ES-3 Elective (Subject) Course 2 50 50 2

30 135 615 750 24

Q

(n

e=lx(tE

?xE}-\2a

Examination

a)

Q0)

'Ag.b;.>5 F

| (,)

lu)

l>ll

lc)ta

co-6-n-"o-put.o.y(CC)eourse

cc-I-11 Core Course-l (PaPer-l l) 3 30 70 t00 3

cc-t-12 Core Course-I @aPer-I2) 3 30 70 100 3

cc-II-r3 Core Coursell (Paper-13) 3 30 70 100 3

cc-II-14 Core Course-ll (PaPer-14) 3 30 70 100 3

Practical Core (PC) Course

PC-I.

tl,l2,l3

&14

Practical Core Coursel

(Paper-l 1,12,13 & l4)

t2 200 200 6

Foundation Course (l'C)

FC-32Compulsory English (L.L,)

2 15 15 50 2

Elective Course @)

2 )U 50 2EG-32 Elective (Generic) Course

2 )U 50 2ES-32 Elective (Subject) Course

30 135 615 750 24

@B-g-

' cc-F7

PAPER.T

BIOPROCESS AND BIOCHEMICAL ENGINEERING

- UNIT.I

Primary and secondarY screening.

. Strain hprovement : Nature of mutation' mutagenesis' isolation of mutants'

.StrainImprovement:App|icationofrecombinantDNAtechniqueinstrainconstruction..'-

. Techniques for preservation and storage of cultures'

UNIT-2

' . Fermenter and bioieactor : Design and types of various fermenters'

. Introduction to Aeration and agitation.

. Basic concept of growth.

. Batch, fed-batch and continuous culture operations, chemostat and turbidostat

. Starter culture, its importance and preparation.

UNIT-3

. Introduction and types of fermentation media

: o Raw materials used in fermentation media.

o Media ootimization.

. Sterilization of media, air and equipments.

UNIT-4

. Overview of downstream processing.

. Fermentationeconomics.- . Fermentation process of alcohol.

. Fermentation process of antibiotlc (penicillin).

nereRrruigs

. L.E. Casida. Industrial Microbiology by.

. Stanbury and Whitaker. Principles offermentation technology.

. Sikyta. Methods in tndustrial microbiology. Ellis Hardwood Ltd

. Krysman. Product recovery in bioprocess technology.

. T.K. Ghose. Bioprocess computation in biotechnology, Ellis Hardwood Ltd,

o bemain et al. (ED) 1999. Manual of industial Microbiology and Biotechnology. Asin Press.

. Do;an (D). Bioprocess Engineering Principles; Academic Press, 1998'

. Cooney, A.E. HumphreY, Comprehensive Biotechnology : The principles and Regulation of Biotechnology

in Industry, Agriculture and Medicine. Vol'2, Pergamon Press' 1985'

@6'^

.

cc-l-8

PAPER-8

MOIECUtAR GENETICS

UNIT.1

. Overview of nucleic acids.

o Alternative forms of DNA'

' o Gehomic organization of prokaryotic and eukaryotic cells'

.DNAasgeneticmateria|:Experimenta|evidences-Transformationprincip|es,Viruses,

Watson and Crick Model.

o Concept of central dogma.

UNIT.2

o Erizymes involved in DNA replication'

o Process of replication : lnitiation, Elongation and Termination'

o Replication of entire DNA molecule; Distinguishing features of DNA replication between

prokaryotes and eukarYotes'

.UNIT-3

o Types of RNA molecules.

.Transcription-lnitiation;RNApo|ymerase;e|ongationandtermination,thedistinguishing

features of the processes in prokaryotes and enkaryotes'

. RNA Processing

. The genetic code

UNIT-4

o Translation-lnitiation, elongation and termination of translation'

o Poittranslationalmodifications'

. Regulation of gene expression, lac-operon'

o Transposable elements : Structure and mechanism of transposition' and Transposable

elements.

. RNA interference : a mode of gene regulation'

66;

-_74frlu/-

R€FERENGES

r tewin 8. (2(x!01 Gene vll IRL Press, OxfordUnh'erslty Press Odord'

. watson, J.D.. Hopkins, Roberts, stie4 w.$H. (1$4 lb|€cular Eiology of the Gene' (4- Edl The

B€niamln/CummllEs Publlshin8Co. Inc California'

. oavis, D.B. Dutbecco, R., Rtsen, H-l{, Ghsbag, lts. (t!t9o} Midoblolo8y, (46 Ed} Harper & Row Publishers,

Slngapore.

. T.A. Bmwn Genome,

o S.B. Prlmms€ Pdnciple of gene maniBdatbn.

/t\

n

/11 ..M A-U'

R,::''ffiaPAPER-9

Principles of Biotechnology Applied to Plants

UNIT.1

o Principles of tissue culture : Historica'l perspectives and development of plant tissue

culture techniques.

. Cell growth and differentiation - morphogenesis.

. concepts of totipotency of cells.

. Laboratory requirements for tissue culture'

UNIT-2

a

a

UNIT-3

a

Culture media : preparations / constituents and concepts of sterilization'

Preparation, lsolation and selection of explants,

LiqLid cell suspension cultures; pollen culture and protoplast culture production and

uses of haploids.

Gene transfer techniques using Agrobacterium.

DNA mediated gene transfer, basics of GMO'

Transgenic plants.

Crop improvement (viral resistance, insect resistance, microbial resistance' herbicide'

tolerance and stress resistance).

UNIT4

. Preservation techniques of germplasm.

. Plant tissue culture and secondary metabolite production'

o Production of svnthetic seeds.

. BT cotton.

REFERENCES

. H.S, Chawla. Intr'oduction to Plant Biotechnologv

. lganacimatha.Basicbiotechnology.

. oas and Mookeriee. Outline of biology.

. David Bourgaize. Biotechnology, Demystifying the concepts Alp 2o0o'

. Eric. S. Grace. Biotechnology unzipped : Promises and realities'

Cohn and Stumph. Outline of BiodEmistrY' Wil€ry eastern'

Miglani. Dictionary of plant tenetics and molecular biology' M\'"a Books'

lganacimatha. Appl. Plant Blotechnology'

K.K. oe. Plaht tissue culture.

Radint and Bholwani. Plant and Ussue qrl&re.

Diion and Gonzales. Plant cell culture. A pracdcal approacl|' IRL Press'

Verpoorte, R. (Ed.l 2qD. Metabolic er€ineering of plant secondary methabolism'

Bernard, R. Gtick and Pasternak. Molecular blotednology.

Eulter and Dawson. cell culture'

n

rA\J/

cc-l-10

PAPER.lO

Principles of Biotechnology Applied to Animals

UNIT.1

o Animal Tissue Culture: History and Scope of Animal Tissue Culture'

. Culture media, Natural and chemically defined media' Serum and Serum free media'

other supplements in media and their use'

. Primary cultures: Primary cultures, Cell lines and lts Maintenance'

. Finite and continuous cell lines, Tissue Disaggregating by Mechanical and Enzymatic

methods, Subculturing'

UNIT.2

o Secondary Culture -transformed animal cells and continuous cell lines'

. Organ culture : Methods of Organ culture' utility of organ culture' Culture of adult

organs.

. Crvopreservation and transport of animal tissue and cell lines'

. Bioreactors: Bioreactor for large scale culture of cells'

UNIT.3

o Expression vector for Animal cell'

o Expression of Cloned proteins in animal cell'

. Overproduction and downstream processing of the expressed proteins'

. Cloning: Overview, Methods of Cloning' Application and Ethics' 'n

vitro fertilization and

embryo transfer, APPlication'

uNtT- ^ ,-.1^.J

. Hybridoma Technology : Hybridoma and monoclonal antibodies' Production' Methods'

Types of Monoclonal Antibodies & Applications'

. Vaccines: Production of Vaccines in animal Cells' Methodology' Application

and limitation.

. Transgenic animals: Techniques for the production of Transgenic Mice' Fish and ship'

Products produced from Transgenic Animals

o Stdm CellTechnology: Overview and Types of Stem Cell' Characteristics of Stem Cell'

Application of Stem cell in Therapy'

REFERENCES

. lganacimatha.Basicbiotechnology'

. Das and Mookeriee. outline of biologY'

. Roy and De. @ll biology.

. Daiid Bourgrize. Biotechnology, Demystlfvhg the concepts' Alp' 2o0o'

Eric. s. Grace. Biotedlnology unzipped : Promises and tealities'

Jan kav' Induction to Animal physiologY' Viva Boo*s'

. Bablnnk and phillp6. 1989. Animal Blotedlnology' Pergamonn'

. Gibert. Devdopmmtal blology.

Jenklus N. 1999. Animal cell biotedrnologl' M€dids and protocols Humana press'

.But|erandwa|ter,1997.Anima|cr||qr|turesandtedfology:Thebasics.|RLpress'

. Masters JRw {ED.) Animal cell culture: A prddlcal appr€d!. 2000' oUP'

. Elements ofElotedlnology : P.K. Gupta'

. Molecular biotechnology : Bernard, R. Glid and Pastemak

. Animal cellculture: Morgan.

Cell culture : gulterand Dawson.

n

B.Sc. BIOTECHNOTOGY

Elective Course (EcXSubiecive)

Semester- 5

PaPer: ECOLOGY AND ECOSYSTEMS

UNiT-1

o Teirestrial Biomes - Grasslands and Forests'

. Aquatic Biomes - Freshwater and Saline water'

. Biogeochemical Cycles-Carbon and Nitrogen cycle'

. . Interaction Within. Between and Among.Populations'.

uNlT-2

. Experimentai Ecosystem Models - Batch system' Flow-Through system'

. Experimental Ecosystem Models - Microcosms'

o MicrobeswithinMacro-communities'

. Structure and Function of some Microbial Communities'

@@

B.Sc. BIOIECHNOIOGY

Elective Course lECXsubjectivel

Semester' 5

Paper : INDUSTRIAI BIOTECHNOTOGY

_ uNlr-l

. Fermentation processes of Amylase.

. Fefmentation processes of Protease.

o Fermentation processes of citric acid.

a . Fermentatign processes of Streptomycin.

UNIT-2

. Fermentation processes of l-Lysine.

. Fermentation processes of Vitamin-Brz.

o Mushrooms

. Single cell Protein.

@

I

B.Sc. BIOTECHNOLOGY

PRACTICAT CORE COURSE

(PAPER-7,8,9 & 101

SEMESTER-5

IIST OF EXPERIMENTS

1". lsolation, Screening and characterization of Amylolytic microbes and Enzymes'

2. lsolation, Screening and characterization of Proteolytic microbes and Enzymes'

3. lsolation,.screening and characterization of Lipolytic microbes and Enzymes'

4. Screening of antibiotic producing microorganisms by Crowded Plate Technique'

5. Screening of antibiotic producing rnicrborganisms by Wilkin's method'

6. BioassaY of Penicillin.

7. ODtimization of medium parameters for the production of Biomass'

8. Optimization of medium parameters for the production Enzyme (Amylases)'

9. Typical fermentation of Alcohol'

10. Typical fermentation of Gluconic acid'

. 11. lsolation of antibiotic resistant mutant(s) bacterium by dir-ect selection (Gradient Plate

Technique)

12.lsolationofantibioticresistantmutant(s)bacteriumbyindirectselection(ReplicaPlate

Technique)

13. sterilitY testing.

14. Sterilization and related techniques used in tissue culture'

- Autoclaving

- Hot Air Oven

Filter Sterilization

- Surfacesterilization

Laminar Air Flow'

15. Preparation of Media and media composition'

16. Introduction of explants for Callusing'

17. characterization of Callus'

18. Sub culturing of Callus'

19. lsolation of genomic DNA from bacterial cells'

20. lsolation of Plasmid DNA'

@

cc-l-11

PAPER-11

Fundamentals of lmmunologY

, UNIT-1

. lnnate.and Acquired immunity'

. Interrelationship between Innate and Acquired immunity'

. Characteristics of the immune response'

. cells & Organs involved in the immune response'

U N tT.z

. Aniigens : Forbignness, High molecular weight,. chemical complexity, Degradability,

Haotens.

. Antigens: Primary and Secondary responses'

.AntigenicityandAntigenbindingsite,EpitopesrecognizedbyB.ce||sandT-cel|s.

. Major classes of antigens, lmmunogenic adjuvant'

uNlT-3

. Antibodv structure and functions.

o Structural features and biological properties of lgc, lgM, lgA, lgD & lgE'

. Generation of antibody diversity'

o Monoclonal antibodY.

UNIT.4

. Antigen-antibody Interactions : Lattice Hypothesis, Agglutination and Precipitation'

. Antigen-antibody interactions : ln vivo and /n vitro interactions between Ag & Ab.

. T-Cell generation, activation and differentiation.

. B-cell generation, activation and differentiation.

REFERENCES

. lM Roitt,J. Brostoff and DK Male (1993). lmmunology. BMP,l-ondon'

. J. Kuby (1991). lmmunology. Freeman and company.

.A.K.Abbas,A.H.Uchtman,J.s.Pober(1994).ce||uIarMo|ecularimmuno|ogy-w,g.saundersco.

Philadelphia.

I

@^

. V.R. Muthukkaruppan, S. Baskar and F. Sinigagtia (1986) Hybridome techniques : A Laboratory Course -'

Macmillan India Limited.

. V.E. Cells (1994). cell Biology Vot-l lmmunology to lll - Academic Press

. lacqueline Sharon. Easic lmmunology by.

@g

cc-l-L2

PAPER-12

GENETIC ENGINEERING

UNIT-1

. . Mechanisms of gene transfer (Processes gene recombination); Transformation,

Transduction and Coniugation.

. Prgteins and enzymes involved in r-DNA technologies'

. Cloning vectors : Plasmids, Phages, Cosmids, YACs'

t Application of linker, adaptor and homopolymer tail in joining diverse DNA molecules'

UNtT.2

. Sanger's method for DNA iequencing.

. Automated DNA sequencing.

. PYrosequencing'

o Microarray based sequencing.

o Chemical and automated DNA Svnthesis.

UNIT.3

. Southern blotting, Western blotting, Northern blotting.

. colony blotting, Dot blotting.

. Hybridization and detection of probe using autoradiography (FISH).

. Cloning strategies: Construction of genomic and cDNA library.

o Screening of Gene in library.

UNIT.4

' . Introduction to genome mapping: use of RFLP, SNP and AFLP.

r Chromosome walking.

- . Applications of rDNA technology: Gene therapy, Expression of therapeutic proteins,

Forensic science.

. Polymerase chain reaction techniques: Basic PCR technique, Variation of PCR techniques

and Applications of PCR.

REFERENCES

o tewin B. (2000) Gene VIL IRL Press, Oxford University Press Oxford.

. Watson, J.D., Hopkins, Roberts, Stiez, Weiner. (1987) Molecutar Biolo8y of the Gene. (4'h Ed) The

Eenjamin/Cummings Publishing Co. Inc. california,

. Davis. D.B. Dulbecco, R., Risen, H.N, Ginsberg. H.S., (1990) Microbiology, (4" Ed) Harper & Row Publishers,

singapore.

T.A. Brown. Genome.

5.8. Primrose. Principle of gene manipulation'

William Bains. Biotechnology from A to Z

Molecular biotechnology, 2nd Ed. Blackwell

Mickios and Freyer. ONA science. A first course in recombinant DNA

Milechell. Introduction to Genetic Algorithms Prentice-Hall

cc-l-13

PAPER-13

Environmental Biotechnology

@'^

UNIT.1

. pollution and contamination of natural components of environment: Define pollution

and contamination; sources of pollutants'

. Tr!nsport and fate of contamination in the environment'

. lsolation and screening of microbes degrading contamination (pollutants) : selective

and enrichment cultivation techniques'

o Biodegradation: Definitions-Ready biodegradability' Ultimate biodegradation'

lnferential biodegradability, Recalcitrant compound' Anthropogenic compounds

(Xenobiotics).

UNIT.2

. An overview of selected compounds : Petroleum hydrocarbons; Alkenes' Cycloalkeanes'

Aromatics, Polycycliq Aromatics & Pesticides'

. Transformation of pesticides - DDT (Dechlorination) to DBP and Biomagnification'

. Reductive dechlorination of PCE & TcE'

. Reductive dechlorination of Petroleum hydrocarbons'

UNIT-3

o Water purif ication-Dwelling supply and Municipal Supply'

o Microbiological analysis of drinking water'

o Role of indicator organisms' W'H'O' microblological standards for drinking water'

o Physical, chemical & Biological properties of Wastewater'

@@

. primary & secondary treatment, (Biological oxidation processes) & T€rtiary treatment

process.

o Treatment of solid wastes (Anaerobic digestion and composting)'

UNIT.4

oBioremediation:typesandoverviewofbioremediationofair,soi|andwater.

. Biofertilizers.

. Bioplastics.

o Bioleaching and MEOR (Microbially enhanced oil recovery)'

REFERENCES

Z-\ :

. . preScott, 1.M., Harley, J.P., Klein. DA., (2002) Microbiology (5$ Ed Y Mccraw Hiil. lnternational Ed.

Tortora, GJ., Funke,8.R., Cate, C.L. (2OoU MicrobiologY: An Introduction (7th Ed) Beniamin cummlngs

N.Y.

. Atlas & Bartha. Microbial Ecology.

. Bruce E. Rittmann and perry L. Mccarty. Environmental Biotechnology: Principles and application,

McGraw- Hill International.

. christson. Manual of Environmental Microbiology, AsM press'

. Eutenia J. Olguin, Gloria Sanchez and Elizabeth Hernandez. Environmental Biotechnology and cleaner

BioProcess, Taylor and Francis.

. Martine Alexander. Biodegradation and Bioremediation'

.PeterMorris(Editor),RikiTherive|.MethodsofEnvironmenta||mpactAssessment.

. Arciwala, S. J. waste water treatment for pollution control' Tata Mccraw'Hill

Publications, New Delhi,

. APHA. standard Methods for the Examination of Water and Wastewater 22nd Ed' (2012)'

o@^

cc-l-14

PAPER.14

Analytical Techniques in Biotechnology

UNIT.1

o Concept of Good Laboraiory Practice and Quality Management'

. Analysis: StePs of Analysis.

. Basic Aspects of Qualitative Analysis'

. Basic Aspect of Quantitative Analysis'

UNIT-2

. Mass Spectroscopy, MALDI.

. Light microscopy Differential interference contrast microscopy'

. Electron microscopY: TEM and SEM'

. Atomic force microscopy and Confocal scanning laser microscopy'

UNIT-3

o lntroductaon to Bioinformatics: History and Overview, Scope of Bioinformatics In

Biotechnology, Bioinformatics and Internet'

. Cotnoonents of Bioinfoimatics : Biological Databases (DNA Database' Protein

Database), overview of Biological sequence analysis - ( Pair wise and Multiple

Alignment), Biological Software' Rasmol'

r Human Genome Project.

.overviewotBioinformaticsApp|ication:Phy|ogenetic,Pharmacogenomics

. (Drug Discovery), Crop Genomics (Agroinfomatics)' Metabolomics ' Chemoinformatics

UNIT.4

. Biosensors: Principles and definition' characteristics of ldeal biosensors'

. . Basic measuring procedure, Biochemical components of biosensors'

. APPlications of Biosensors'

oImmobi|ization:Basicconceptofimmobi|izationinbiotechno|ogy,Princip|esand

mechanism ot lmmobilization' Methods of lmmobilization'

. Bioreactor for lmmobilization: Bioreactor and their Types'

REFERENCES

.WiIson&WaIker.1995.PrincipIesandtechniquesofpracticaIBiochemistry.cambridgeUniv.Press.

. Davidson V.L. & Sistman. 1993 Biochemistry'

@@

Blood et at' 1996' l-aboratorY DNA science. Benjamin'

goyer, 2001. Modem Experimentat biochemistry, 3/e, Addison'

. Becker. 1996. Biotedlnology: A laboratory course. Alp.

. Plummer. An introdudion to practical Biochemistry.

. J. Jayraman,l-ab Manualin Biochemistry.

. Tinoco land et al. 1995. Physical chemistry Principles and applications in biological Sciences, Prentice-Hall,

Switzer and Gauity' 1995. €xperimental Biochemistry. W H Freeman.

. voet Donald. 1999. Fundamentals of Biochemistry.

I Athel Cornish Bowder. 1999. Basic mathematics for biochemistry. OUP.

Elliott & Elliol 2001. Biochemistry and molecular biology, OUP.

. sidiriin and Moore, 2OOO. Basic laboratory methods for biotedlnology, Longman.

. Bloinformadcs - Managing Scientific Data, Zoe' Lacroix and Terence Critdllow.

. Bioinformatics - Sequencg structure and Databanks, Des HlSglns & Wllie Taylor.

n

i

6t @'n

B.Sc. BIOTECHNOIOGY

Elective Course (ECXSubiective)

Semester- 5

Paper : DYSFUNCIONAL IMMUNIW

UNIT.l

a

a

UNIT-2

. lmmediateHYPerseirsitivitY'

. DeiaYedHYPersensitivity'

. oncogenes and Cancer Induction'

. CancerlmmunotheraPY'

.!I

B.Sc. BPTECHNOTOGY .

Ehctive Course (ECXSubiectivel

Semester- 6

Paper : DAIRY BIOTECHNOU)GY

. UNIT-I

o Nutritional value of Milk.

. Pasteurization of Milk.

^ . Biochemlcal Types of Microorganisms in Milk.n. . Pathogenic Types of gacteria in Milk.

UNIT.2 .

.. . Starter Cultures used in dairy industry.

. Fermenteddairyproducts.

. Cheese production.

. Types of Cheese.

A

@o

-\-----/

B.Sc. BIOTECHNOLOGY

PRACTICAT CORE COURSE

(PAPER-11, 12, 13 & 14)

SEMESTER-6

LIST OF EXPERIMENTS

1. To study water sampling techniques and sample preservation'

2. Determination of Total Solids (TS), Total Dissolved Solids (TDS) and Total Suspended Solids

(rss).

3. Estimation of Dissolved Oxygeh (DO) from the given water sample 'L

4. Estimation of Biological Oxygen Demand (BOD) from the glven water sample'

5. Estimation of POr-P from the given water sample'

6. Estimation of NO:-N from the given water sample'

7. Estimation of NO2-N from the given water sample'

8. Estimation of Chloride from the given water sample

9. Estimation of Sulfate from the given water sample'

10. BacterioloSical analysis of water by Most Probable Number (MPN) technique'

11. lsolation of non-symbiotic nitrogen fixers from soil'

12. lsolation of symbiotic nitrogen fixers'

13. Study of air microflora'

14. Total count of White Blood cells (WBcs)'

15. Total count of Red Blood Cells (RBCs)'

16. Differential Count of White Blood Cells (WBCs)'

17. Estimation Hemoglobin by Sahli's Method'

-- 18. Blood grouping

19. WIDAL test (Slide Test)

- 20. Agarose electrophoresis of DNA'

zt. preparation bf competent cells and transformation of plasmid DNA'

22. Quantification of DNA by spectrophotometry'

23. Usage of NcBl resources for Biological Information'

24. lmmobilization of enzyme'

25. lmmobilization of cells'

Itbl'2-t ,^.

X