fads1 fads2 gene cluster, pufa intake and blood lipids in children. results from the giniplus and...
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![Page 1: FADS1 FADS2 gene cluster, PUFA intake and blood lipids in children. Results from the GINIplus and LISAplus studies. Marie Standl Helmholtz Zentrum München](https://reader036.vdocuments.net/reader036/viewer/2022062716/56649e045503460f94aefd31/html5/thumbnails/1.jpg)
FADS1 FADS2 gene cluster, PUFA intake and blood lipids in children.
Results from the GINIplus and LISAplus studies.
Marie Standl
Helmholtz Zentrum München Institute of Epidemiology I
Bristol, 18/10/11
![Page 2: FADS1 FADS2 gene cluster, PUFA intake and blood lipids in children. Results from the GINIplus and LISAplus studies. Marie Standl Helmholtz Zentrum München](https://reader036.vdocuments.net/reader036/viewer/2022062716/56649e045503460f94aefd31/html5/thumbnails/2.jpg)
Background
Cardiovascular diseases
• Total cholesterol
• High-density lipoprotein (HDL)
• Low-density lipoprotein (LDL)
• Triglycerides
Dietary PUFA intake
FADS Genes
![Page 3: FADS1 FADS2 gene cluster, PUFA intake and blood lipids in children. Results from the GINIplus and LISAplus studies. Marie Standl Helmholtz Zentrum München](https://reader036.vdocuments.net/reader036/viewer/2022062716/56649e045503460f94aefd31/html5/thumbnails/3.jpg)
Fatty acid metabolism
Omega 6fatty acid
AA(Arachidonic acid)
“pro inflammatory”
Omega 3fatty acid
DHA(Docosahexaenoic
acid)
“beneficial effects”
FADS Genes
Major allele
Minor allele
More product
More substrate
![Page 4: FADS1 FADS2 gene cluster, PUFA intake and blood lipids in children. Results from the GINIplus and LISAplus studies. Marie Standl Helmholtz Zentrum München](https://reader036.vdocuments.net/reader036/viewer/2022062716/56649e045503460f94aefd31/html5/thumbnails/4.jpg)
Background
Cardiovascular diseases
• Total cholesterol
• High-density lipoprotein (HDL)
• Low-density lipoprotein (LDL)
• Triglycerides
Dietary PUFA intake
FADS Genes
Minor allele:
Total cholesterol, HDL, LDL
Triglycerides
![Page 5: FADS1 FADS2 gene cluster, PUFA intake and blood lipids in children. Results from the GINIplus and LISAplus studies. Marie Standl Helmholtz Zentrum München](https://reader036.vdocuments.net/reader036/viewer/2022062716/56649e045503460f94aefd31/html5/thumbnails/5.jpg)
Background
• Total cholesterol
• High-density lipoprotein (HDL)
• Low-density lipoprotein (LDL)
• Triglycerides
Dietary PUFA intake
FADS Genes
Objective
• Are lipid levels already
determined by the
FADS genotype in
school-aged children?
• Interaction between
dietary PUFA intake
and FADS genotype?
![Page 6: FADS1 FADS2 gene cluster, PUFA intake and blood lipids in children. Results from the GINIplus and LISAplus studies. Marie Standl Helmholtz Zentrum München](https://reader036.vdocuments.net/reader036/viewer/2022062716/56649e045503460f94aefd31/html5/thumbnails/6.jpg)
Methods
• Dietary n3 PUFA intake:
• Derived from FFQ (food frequency questionnaire)
• n3 PUFA = (ALA+EPA+DPA+DHA)/total dietary energy intake
• Results presented as mg/MJ per interquartile range increase
• FADS genes: six SNPs were genotyped (results presented for rs174556 and
rs174575)
• Linear regression models:
• Total cholesterol, LDL, triglyceride: results presented as means ratios (log NV)
• HDL: results presented as estimate β and standard deviation (Sd)
• Adjustment: gender, study centre, age, BMI, fasting status and total dietary energy intake
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Study population(with genetic information)
N=2006 % or Median (Qu. 25%/Qu. 75%)
Study
GINI 64%
LISA 36%
Fasting blood samples 18%
Age [years] 10.2 (10.1/10.3)
BMI [kg/m²] 17 (16/19)
n-3 PUFA intake [mg/MJ]0.14 (0.13/0.16)
Total cholesterol [mmol/L] 4.79 (4.28/5.32)
HDL [mmol/L] 1.24 (1.06/1.44)
LDL [mmol/L] 2.12 (1.72/2.53)
Triglyceride [mmol/L] 1.19 (0.90/1.64)
![Page 8: FADS1 FADS2 gene cluster, PUFA intake and blood lipids in children. Results from the GINIplus and LISAplus studies. Marie Standl Helmholtz Zentrum München](https://reader036.vdocuments.net/reader036/viewer/2022062716/56649e045503460f94aefd31/html5/thumbnails/8.jpg)
Results: Total cholesterol
A: major allele / a: minor allele
![Page 9: FADS1 FADS2 gene cluster, PUFA intake and blood lipids in children. Results from the GINIplus and LISAplus studies. Marie Standl Helmholtz Zentrum München](https://reader036.vdocuments.net/reader036/viewer/2022062716/56649e045503460f94aefd31/html5/thumbnails/9.jpg)
Results: LDL
A: major allele / a: minor allele
![Page 10: FADS1 FADS2 gene cluster, PUFA intake and blood lipids in children. Results from the GINIplus and LISAplus studies. Marie Standl Helmholtz Zentrum München](https://reader036.vdocuments.net/reader036/viewer/2022062716/56649e045503460f94aefd31/html5/thumbnails/10.jpg)
Results: Triglyceride
A: major allele / a: minor allele
![Page 11: FADS1 FADS2 gene cluster, PUFA intake and blood lipids in children. Results from the GINIplus and LISAplus studies. Marie Standl Helmholtz Zentrum München](https://reader036.vdocuments.net/reader036/viewer/2022062716/56649e045503460f94aefd31/html5/thumbnails/11.jpg)
Results: HDL
rs174556 n3 PUFA [mg/MJ] AA (ref) Aa (ref AA) aa (ref AA)
Estimate 0.02 - -0.04 0.00
Sd 0.01 - 0.02 0.03
p-value 0.017 - 0.007 0.890
rs174575 n3 PUFA [mg/MJ] AA (ref) Aa (ref AA) aa (ref AA)
Estimate 0.02 - -0.01 -0.01
Sd 0.01 - 0.01 0.03
p-value 0.014 - 0.332 0.691
A: major allele / a: minor allele
![Page 12: FADS1 FADS2 gene cluster, PUFA intake and blood lipids in children. Results from the GINIplus and LISAplus studies. Marie Standl Helmholtz Zentrum München](https://reader036.vdocuments.net/reader036/viewer/2022062716/56649e045503460f94aefd31/html5/thumbnails/12.jpg)
Further results
• No interaction between n3 PUFA and FADS genotype
• No association between lipid levels and n6 PUFA intake
• Similar results for the n3 PUFAs ALA, EPA, DPA and DHA
• Similar results in a stratified analysis for LISA and GINI
![Page 13: FADS1 FADS2 gene cluster, PUFA intake and blood lipids in children. Results from the GINIplus and LISAplus studies. Marie Standl Helmholtz Zentrum München](https://reader036.vdocuments.net/reader036/viewer/2022062716/56649e045503460f94aefd31/html5/thumbnails/13.jpg)
Summary
• n3 PUFA:
• Total cholesterol, HDL and LDL
• Triglycerides
• Minor allele of FADS genotype:
• Total cholesterol, HDL and LDL
• Triglycerides
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Acknowledgements
Eva Lattka
Barbara Stach
Sibylle Koletzko
Carl-Peter Bauer
Andrea von Berg
Dietrich Berdel
Ursula Krämer
Beate Schaaf
Stefan Röder
Olf Herbarth
Anette Buyken
Tim Drogies
Joachim Thiery
Berthold Koletzko
Joachim Heinrich
![Page 15: FADS1 FADS2 gene cluster, PUFA intake and blood lipids in children. Results from the GINIplus and LISAplus studies. Marie Standl Helmholtz Zentrum München](https://reader036.vdocuments.net/reader036/viewer/2022062716/56649e045503460f94aefd31/html5/thumbnails/15.jpg)
Characteristics of the FADS SNPs
• Results presented for rs174556 and rs174575
• Genotype frequency
aa aA AA
rs174556 9% 41% 50%
rs174575 7% 37% 56%
a: minor alleleA: major allele