featured assay - covance assay total testosterone by lc/ms/ms covance’s total testosterone by...
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Featured Assay
Total Testosterone by LC/MS/MS
Covance’s total testosterone by liquid chromatography and tandem mass spectrometry (LC/MS-MS) is a highly sensitive and specific method that is certified by the Centers for Disease Control (CDC) Hormone Standard-ization (HoST) Program. This assay provides a more accurate testosterone measurement at very low concentra-tions common among women, children, and hypogonadal men.
Ultra-high Sensitivity Using State of the Art Instrumentation
Covance has validated two total testosterone LC/MS-MS methods: One method with a curve range from 2 to 2,000 ng/dL, and an ultra-sensitive method with a curve range from 0.1 to 100 ng/dL.
Representative chromatogram of a calibrator at the LLOQ of 0.1 ng/dL (1.0 pg/mL):
This level of sensitivity is achieved using the most advanced UHPLC/MS-MS technology available: -Shimadzu Nexera Ultra-High Performance Liquid Chromatograph (UHPLC)-AB Sciex QTRAP 5500 triple quadrupole mass spectrometer
DEC2011
Featured Assay
CDC Certification
Covance’s total testosterone assay meets the stringent CDC Hormone Standardization (HoSt) Program criteria established in 2010. Covance obtained CDC certification on November, 2011. This certification will be pub-lished on the CDC Web site at www.cdc.gov/labstandards/doc/HoSt Report.pdf.
The Endocrine Society along with 14 professional and medical organizations have endorsed CDC’s HoSt pro-gram. The primary goal of this program is to improve the accuracy and precision of steroid hormone tests used in patient care and research for testosterone, and estradiol.
The program is a year long voluntary certification process that consists of 2 phases:
DEC2011
Featured Assay
Covance obtained a mean bias of 2.8% which is well within the CDC predefined bias criteria of 6.4%.
CDC certification positions Covance’s methodology among the benchmark assays for testosterone measurement.
DEC2011
Covance Central Laboratory Services
*Special Collection DEC2011
Panels MethodologyChemistry Panel: ALT (SGPT), AST (SGOT), Albumin, Calcium, Chloride, Alkaline Phos-phatase, Bicarbonate, Cholesterol, CK, Creatinine, Direct Bilirubin, LDH, Magnesium, Globulin, Glucose, GGT, Indirect Bilirubin, Phosphorus, Potassium, Sodium, Total Bilirubin, Total Protein, Triglycerides, Urea Nitrogen, Uric Acid
Varies by Analyte
Hematology Hemogram Panel:Hematocrit, Hemoglobin, MCH, MCHC, MCV, Platelets, RBC, WBC, RBC Morphology
Varies by Analyte
Hematology Differential Panel: Basophils, Eosinophils, Lymphocytes, Monocytes, Neutrophils
Varies by Analyte
Urinalysis Macroscopic Panel: Bilirubin, Blood, Clarity, Color, Glucose, Ketones, Leucocyte Esterase, Nitrite, pH, Protein, Specific Gravity, Urobilinogen
Varies by Analyte
Urinalysis Microscopic Panel: RBC, WBC, Casts, Crystals, Bacteria, Epithelial Cells, Yeast, Oval Fat Bodies, Fat, Mucous, Sperm, Trichomonas
Microscopy
Flow Cytometry Panel (EDTA Whole Blood) CD3, CD4, CD8, CD19, CD 16+56, CD3+4+8, CD4/CD8 Ratio, CD3/CD19 Ratio
Flow Cytometry
Urine Drug Screen - Confirmations performed in Indy/GenevaAntidepressants, Opiates, Cocaine, Benzodiazepines, Amphetamines, Barbiturates, Cannabinoids, Methadone, Phencyclidine and Propoxy-phene
Varies by Analyte
SSRI ScreenCitalopram and/or metabolite, Fluoxetine and/or metabolite,Fluvoxamine, Paroxetine, Sertraline and/or metabolite, Venlafaxine and/or metabolite and Trazodone
GC/MS
CDC Lipid Research PanelCDC Cholesterol, CDC Triglycerides (Glycerol Blanked), CDC HDL Cholesterol
Enzymatic
*Special Collection DEC2011
Covance Central Laboratory Services
Analytes Methodology17 Alpha Hydroxyprogesterone Radioimmunoassay
Acetaminophen (Paracetamol) Previously validated, currently inactive
Acid Labile Subunit Previously validated, currently inactive
Acid Phosphatase/ Total and Prostatic Previously validated, currently inactive
Activated Partial Thromboplastin Time, APTT Photometric Clot Detection
Activated Protein C Resistance Clot Detection
Active Androstanediol Glucuronide (3a-diol G) Previously validated, currently inactive
Adenovirus Antigen Serotypes 40/41 Enzyme Immunoassay
Adiponectin Enzyme Immunoassay
Adiponectin Multiplex Immunoassay
Adiponectinm HMW (High Molecular Weight) Enzyme Immunoassay
ADMA, Asymmetric dimethylarginine Enzyme Immunoassay
Adrenocorticotropic Hormone, ACTH ECLIA - Electrochemiluninescence Immunoassay
Alanine Aminopeptidase, AAP Previously validated, currently inactive
Alanine Aminotransferase, ALT Enzymatic
Albumin Colorimetric
Albumin, CSF Nephelometry
Aldolase Previously validated, currently inactive
Aldosterone LC/MS/MS
Aldosterone Radioimmunoassay
Alkaline Phosphatase Colorimetric
Alkaline Phosphatase, Bone Specific Chemiluminescence
Alkaline Phosphatase Isoenzymes Colorimetric-Heat Inactivation (validating)
Alpha 1 Acid Glycoprotein, AAG Nephelometry
Alpha-1-Antitrypsin, AAT Nephelometry
Alpha 1 Microglobulin Nephelometry
Alpha 2 Macroglobulin Nephelometry
Alpha Crosslaps, Urine Enzyme Immunoassay
Alpha-Fetoprotein, AFP Chemiluminescence
Alpha Glutathione S Transferase Enzyme Immunoassay
Amylase, Total Enzymatic
Amylase, Pancreatic Enzymatic
Amyloid A Enzyme Immunoassay
Androstenedione LC/MS/MS (validating)
Angiotensin Converting Enzyme Previously validated, currently inactive
Angiotensin II Radioimmunoassay
Anti-cardiolipin IgG Enzyme Immunoassay
Anti-cardiolipin IgM Enzyme Immunoassay
Anti-CCP (Cyclic Citrullinated Peptide) ECLIA-Electrochemiluminescence
Anti-Double Stranded DNA, IgG Fluorescent Microparticle Immunoassay
Antimicrobial Susceptibility Testing (Customized MICs, Agar dilution,Clindamycin Disk induction, yeast MICs, Kirby Bauer, E-test, SIT/SBT, Hodge test, etc)
MIC/KB
Antinuclear Antibody, ANA Indirect Fluorescent Antibody
Anti-pneumococcal Capsular Polysaccaride, IgG Enzyme Immunoassay
Antistreptolysin O Antibody, ASO Previously validated, currently inactive
Covance Central Laboratory Services
*Special Collection DEC2011
Analytes MethodologyAntithrombin III Activity Chromogenic
Antithrombin III Previously validated, currently inactive
Apolipoprotein A-1 Nephelometry
Apolipoprotein B Nephelometry
Apolipoprotein B48 Enzyme Immunoassay (validating)
Apolipoprotein B Total Nephelometry
Apolipoprotein B100 (Calculation only): Apolipoprotein B Total - Apolipoprotein B48
Calculation only
Apolipoprotein A2 Turbidimetric Immunoassay
Apolipoprotein A4 Enzyme Immunoassay (validating)
Apolipoprotein C2 Previously validated, currently inactive
Apolipoprotein C3 Turbidimetric Immunoassay
Apolipoprotein E Turbidimetric Immunoassay
Arginine Vasopressin, ADH Previously validated, currently inactive
Aspartate Aminotransferase, AST Enzymatic
Bacteriology - cultures (Sputum, urine, stool, etc) and IsolateIdentification
Microbiology
BDNF, Brain-derived Neurotrophic Factor Previously validated, currently inactive
Beta Carotene Previously validated, currently inactive
Beta Crosslaps ECLIA - Electrochemiluninescence
Beta Human Chorionic Gonadotropin, B-hCG, Qualitative Chemiluminescence
Beta Hydroxybutyrate Previously validated, currently inactive
Beta 2-Microglobulin Nephelometry
Beta Quantification - LDL, VLDL by ultracentrifugation Ultracentifugation/Enzymatic
Bicarbonate Enzymatic
Bile Acids Enzymatic
Bilirubin, Direct Colorimetric
Bilirubin, Total Colorimetric
Blood Urea Nitrogen, BUN Enzymatic
Bone Specific Alkaline Phosphatase Chemiluminescence
BRAF Mutation Validating
Brain Natriuretic Peptide, BNP Chemiluminescence
CA -125 Chemiluminescence
CA 19-9 Chemiluminescence
CA 27.29 Previously validated, currently inactive
Calcitonin Chemiluminescent Immunometric
Calcium Colorimetric
Calcium, Urine Colorimetric
Calprotectin (See Fecal Calprotectin)
Carbamazepine Previously validated, currently inactive
Carbamazepine and 10,11 Epoxide Previously validated, currently inactive
Carbamazepine, Free Previously validated, currently inactive
Carbohydrate Deficient Transferrin % Previously validated, currently inactive
Carboxyhemoglobin Spectrophotometry
Covance Central Laboratory Services
*Special Collection DEC2011
Analytes MethodologyCarboxyterminal telopeptide Type I Collagen, ICTP Radioimmunoassay
Carcinoembryonic Antigen, CEA Chemiluminescence
Carnitine, Free Previously validated, currently inactive
Cartilaps (CTX-II), C-terminal telopeptides of type II collagen, Urine Previously validated, currently inactive
Catecholamines - Norepinephrine only Previously validated, currently inactive
CCR5 Receptor Occupancy Validating
CCR5 Delta 32 RealTime PCR Laboratory Developed Method
CD138 Magnetic Cell Separation (Bone Marrow) Miltenyi Biotec Microbeads
CD34 Magnetic Cell Separation (Bone Marrow) Miltenyi Biotec Microbeads
CD34 Magnetic Cell Separation (Whole Blood) Miltenyi Biotec Microbeads
CD19 Magnetic Cell Separation Miltenyi Biotec Microbeads
CD4/CD8 - See Flow Cytometry Panel
CD45RA, CD45RO, etc - See Flow Cytometry Custom Markers
CellSearch Assay, Circulating Tumor Cells Previously validated, currently inactive
Cell Separation / Enrichment - RosetteSep Product Rosette Sep Method
Centromere B Antibodies Previously validated, currently inactive
Ceruloplasmin Nephelometry
cAMP (Cyclic Adenosine Monophosphate) Previously validated, currently inactive
cGMP (Cyclic Guanosine Monophosphate) Previously validated, currently inactive
Chlamydia Pneumoniae Antibody IgA Immunofluorescence
Chlamydia Pneumoniae Antibody IgG Immunofluorescence
Chlamydia Pneumoniae Antibody IgM Immunoflourescence
Chlamydia Trachomatis DNA Chemiluminescence
Chloride Ion Selective Electrode
Chloride, Urine Ion Selective Electrode
Cholesterol Enzymatic
Cholesterol, HDL Enzymatic Colorimetric
Cholesterol, HDL2 and HDL3 Previously validated, currently inactive
Chylomicron Remnants (calulation only)
CK-18 Fragments (Cytokeratin - PEVIVA M30 Apoptosense Kit) Enzyme Immunoassay
CK-18 Total (Cytokeratin - PEVIVA M65 Apoptosense Kit) Enzyme Immunoassay
CK Isoenzymes Electrophoresis
CK-MB Chemiluminescence
Clostridium Difficile Cytotoxicity Cell Culture
Clostridium Difficile Toxin Enzyme Immunoassay
Clusterin Enzyme Immunoassay (active for serum only)
Complement C3 Nephelometry
Complement C4 Nephelometry
Complement, Total, CH50 Hemolytic Assay
Cortisol Chemiluminescence
Cortisol Previously validated, currently inactive
Cortisol, Urinary Free High Performance Liquid Chromatography
Corticosteroid Binding Globulin Previously validated, currently inactive
Cotinine , Serum (Qualitative or Semiquantitative) Enzyme Immunoassay
Cotinine, Serum LC/MS/MS (validating)
Cotinine, Urine Enzyme Immunoassay
C-Peptide Chemiluminescence
C-Reactive Protein, CRP Nephelometry
Creatine Kinase, CK Enzymatic
Covance Central Laboratory Services
*Special Collection DEC2011
Analytes MethodologyCreatine, Jaffe Colometric / Rate-blanked
Creatinine, Urine Colormetric (TKffe)
Creatinine, Enzymatic Enzymatic
Creatinine, Enzymatic (Urine) Enzymatic
Crosslaps, Type I Collagen Specific (CTX) ELISA
Crosslaps, Type I Collagen Specific (CTX), Urine Previously validated, currently inactive
CXCL12 (See SDR-1 alpha)
Cyclosporine Previously validated, currently inactive
CYP2C9 xTAG Mutation Detection
CYP2C19 xTAG Mutation Detection
CYP2D6 Autogenomics Infiniti - Validating
CYP3A5 Validating
Cystatin C Nephelometry
Cystatin C, Urine Enzyme Immunoassay
Cytokine Inflammatory Panel by Flow Cytometry (CBA) - Can include 1or all: IL-1 Beta, TNF alpha, IL-6, IL-8, IL-10 and IL-12 (p70)
Flow Cytometry
Cytokine TH1/TH2 Panel by Flow Cytometry (CBA) - Can included 1 orall: TNFalpha, IFN gamma, IL-2, IL-4, IL-5 and IL-10
Flow Cytometry
Cytomegalovirus, CMV IgG Chemiluminescence
Cytomegalovirus, CMV IgM Chemiluminescence
Cytomegalovirus (CMV) PCR Validating
Cytopathology *** Preferred Relationship with Reference Lab Consult Medical Affairs
D-Dimer Enzyme Immunoassay
Deoxypyridinoline Enzyme Immunoassay
DHEA - Dehydroepiandrosterone LC/MS/MS
DHEA-S, Dehydroepiandrosterone Sulfate Chemiluminescence
DHPG - Dihydroxyphenylglycol (must be performed with Catecholamines)
Previously validated, currently inactive
DHT, Dihydrotestosterone LC/MS/MS
Digoxin Chemiluminescence
Dilute Russell Viper Venom Test Screen and confirmation (LupusAnticoagulant)
Photometric Clot Detection
Diphtheria Antitoxoid Antibody, IgG Enzyme Immunoassay
DNA Isolation DNA Isolation
DNA Isolation, Bone Marrow Validating
DNA Isolation, Buccal Cells Validating
DNA Extraction, Granulocytes QIAamp DNA Blood
DNA Isolation, Saliva DNA Isolation
DNase-B Antibodies Previously validated, currently inactive
EBV VCA, IgG (Epstein-Barr Virus-Viral Capsid Antigen, IgG) Chemiluminescence
EBV VCA, IgM (Epstein-Barr Virus-Viral Capsid Antigen, IgM) Chemiluminescence
Ecarin Clotting Time, ECT Photometric Clot Detection
EGFR Mutation Real-time PCR
ENA-78 (Epithelial Neutrophil Activating Peptide 78) Enzyme Immunoassay
Endogenous Thrombin Potential, ETP Previously validated, currently inactive
Endomysial Antibody, IgA Validating
Endothelin 1 Enzyme Immunoassay
Eosinophil Cationic Protein (ECP) Previously validated, currently inactive
Eosinophil Protein X Previously validated, currently inactive
Eosinophil Stain Light Microscopy
Covance Central Laboratory Services
*Special Collection DEC2011
Analytes MethodologyErythrocyte Sedimentation Rate (ESR) Modified Westergren
Erythropoietin Chemiluminescent Immunometric
E-Selectin Mulitplex Immunoassay
Estradiol Chemiluminescence
Estradiol LC/MS/MS
Estrone LC/MS/MS
Estrone sulfate Previously validated, currently inactive
Ethanol, Qualitative GC/FID
Ethanol, Quantitative GC/FID
Ethosuximide Previously validated, currently inactive
Factor II Mutation Genotyping Light Cycler
Factor V Genotyping Light Cycler
Factor VII Activity Previously validated, currently inactive
Factor VIII Activity Previously validated, currently inactive
Factor Xa (Low Molecular Weight Heparin) Previously validated, currently inactive
Fecal Calprotectin Enzyme Immunoassay
Fecal Leukocytes (See Lactoferrin)
Fecal Occult Blood Color Development
Fecal Occult Blood Chromatographic Immunoassay
Ferritin Chemiluminescence
FGF-23 C-terminal Enzyme Immunoassay
FGF-23 Intact Enzyme Immunoassay
Fibroblast Growth Factor Basic, FGF Basic Previously validated, currently inactive
Fibrinogen Degradation Product, FDP Previously validated, currently inactive
Fibrinogen Photometric Clot Detection
Flow Cytometry Custom Markers:CCR5 FoxP3 TregCD3/CD8/CD28/CD57CD16/56 (NK)NK-Cell ActivityNKT-Like CellsCD8/CD38/HLADR (T cells)CD4/CD38/HLADR (T cells)CD4/25 (T cells)CD7/26 (T cells)CD45/CD14/CD16CD20/22 (B cells)CD19/27 (B cells)CD19/27 (B cells)CD19/lgD/27/38 (B cells)CD19/lgD/27/38 (B cells)CD45/CD19/CD5/CD38 (B cells)CD3/CD10/Kappa/Lambda/CD19/CD45SEMA4DIgG4 PanelNK CD69 Panel
Flow Cytometry
Folate Chemiluminescence
Folate, RBC Previously validated, currently inactive
Follicle Stimulating Hormone, FSH Chemiluminescence
Free Fatty Acids Non-esterified Enzymatic
Free Insulin Previously validated, currently inactive
Free Light Chains (Freelite), Kappa and Lambda Nephelometry
Free PSA Chemiluminescence
Free T3 Chemiluminescence
Covance Central Laboratory Services
*Special Collection DEC2011
Analytes MethodologyFree T4 Chemiluminescence
Free Testosterone (%) LC/MS/MS Equilibrium Dialysis
Fructosamine Colorimetric
Gabapentin Previously validated, currently inactive
Gamma Glutyamyl Transferase, GGT Colorimetric
Gastrin Chemiluminescent Immunometric
Gene Resistance Testing, PCR: PVL, MecA and SEC Mec Polymerase Chain Reaction
Gentamicin Previously validated, currently inactive
Giardia Specific Antigen Previously validated, currently inactive
Glucagon Radioimmunoassay
Glucagon Like Peptide-1, Total (GLP-1 Total) Microparticle Immunoassay
Glucose Enzymatic
Glucose, Urine Colorimetric
Glutamic Acid Decarboxylate Antibodies, GAD Antibodies Radioimmunoassay
Glycerol, Free Previously validated, currently inactive
“GlycoMark”, 1,5 Anhydroglucitol Enzymatic
Gram Stain Light Microscopy
Growth Hormone Chemiluminescence
Growth Hormone (For Pediatric Studies) Chemiluninescent -Immunometric
Haptoglobin Nephelometry
HDL Cholesterol PEG Enzyme Colorimetric
HDL Lipoparticle Size Gradient Gel Electrophoresis
Helicobacter Pylori, IgG Previously validated, currently inactive
Haemagglutination Titer Test (HA Titer Test) for Influenzae A&B Consult Lab
Hemoglobin A1c High Performance Liquid Chromatography
Hepatitis A Antibody Chemiluminescence
Hepatitis A IgM Antibody Chemiluminescence
Hepatitis B Core Antibody Chemiluminescence
Hepatitis B Core IgM Antibody Chemiluminescence
Hepatitis Be Antibody Enzyme Immunoassay
Hepatitis Be Antigen Enzyme Immunoassay
Hepatitis B Surface Antibody, Qualitative Chemiluminescence
Hepatitis B Surface Antibody, Quantitative Chemiluminescence
Hepatitis B Surface Antigen Chemiluminescence
Hepatitis B Viral DNA, Quantitative Real-Time Quantitative PCR
Hepatitis B Genotyping Line Probe Assay (validating)
Hepatitis C Antibody Chemiluminescence
Hepatitis Delta Antibody Previously validated, currently inactive
Hepatitis C Viral RNA, Quantitative Real-Time Quantitative PCR (TaqMan)
Hepatitis C Viral RNA, Quantitative Real-Time Quantitative PCR (Abbott)
Hepatitis C Genotyping DNA Sequencing
Hepatitis C Genotyping Line Probe Assay
Hepcidin, Urine Previously validated, currently inactive
HER-2/neu Chemiluminescence
Herpes Simplex Screen, IgG Previously validated, currently inactive
HHPV-8 (Human Herpes Virus-8), Qualitative RT-PCR, ABI 7900
Histamine Previously validated, currently inactive
Covance Central Laboratory Services
*Special Collection DEC2011
Analytes MethodologyHistone Antibodies Previously validated, currently inactive
HIV-1 Genotyping Previously validated, currently inactive
HIV-1(RNA) PCR Real time PCR (TaqMan)
HIV-1(RNA) PCR Real-Time Quantitative PCR (Abbott)
HIV-1 Western Blot Western Blot
HIV-1/HIV-2 Antibody Chemiluminescence
HLA B27 Genotyping To be determined (validating)
HLA B5701 Genotyping To be determined (validating)
Homocysteine Chemiluminescence
HTR2A rs7330461 Genotype Real time PCR Laboratory Developed
HTLV I/HTLVII Antibodies Previously validated, currently inactive
HTLV I/HTLVII confirmation assay Previously validated, currently inactive
Human Anti Mouse Antibody, IgG, HAMA Previously validated, currently inactive
Hyaluronic Acid Previously validated, currently inactive
iBig Endothelin Previously validated, currently inactive
ICTP, [see Carboxyterminal Telopeptide Type I Collagen]
IL-1 a ( Interleukin 1 Alpha) Previously validated, currently inactive
IL-1B, Human Interleukin 1 Beta See Luminex Bio-Rad Pro Human Cytokine 10-Plex Panel
IL-1ra, Human IL-1 Receptor antagonist Enzyme Immunoassay
IL-2 See Luminex Bio-Rad Pro Human Cytokine 10-Plex Panel
IL-2 sR alpha (Interleukin 2 Soluble Receptor Alpha) Enzyme Immunoassay
IL-4 See Luminex Bio-Rad Pro Human Cytokine 10-Plex Panel
IL-5 See Luminex Bio-Rad Pro Human Cytokine 10-Plex Panel
IL-6 See Luminex Bio-Rad Pro Human Cytokine 10-Plex Panel
IL-6, Interleukin 6 Enzyme Immunoassay
IL-6 s Receptor Previously validated, currently inactive
IL-8 Enzyme Immunoassay
IL-10, Interleukin 10 See Luminex Bio-Rad Pro Human Cytokine 10-Plex Panel
IL-12 p40 Previously validated, currently inactive
IL-12 p70 See Luminex Bio-Rad Pro Human Cytokine 10-Plex Panel
IL-12 Total, Interleuking 12 Previously validated, currently inactive
IL-13, Interleukin 13 See Luminex Bio-Rad Pro Human Cytokine 10-Plex Panel
IL-15, Interleukin 15 Previously validated, currently inactive
IL-17, Interleukin 17 Enzyme Immunoassay
IL-17F, Interleukin 17F Previously validated, currently inactive
IL-28b Mutation RealTime PCR, Laboratory Developed
Immunofixation, Myeloma Studies Immunofixation
Immunofixation, Myeloma Studies (Urine) Immunofixation
Immunoglobulin A, IgA Nephelometry
Immunoglobulin D, IgD Nephelometry
Immunoglobulin E, IgE Chemiluminescence
Immunoglobulin G, IgG Nephelometry
Immunoglobulin G, IgG (urine) Nephelometry
Immunoglobulin G Subclasses (IgG Subclasses) Nephelometry
Immunoglobulin M, IgM Nephelometry
Influenza A IgG Antibody Enzyme Immunoassay
Influenza B IgG Antibody Enzyme Immunoassay
Influenza Virus detection by PCR Artus Real-Time PCR
Covance Central Laboratory Services
*Special Collection DEC2011
Analytes MethodologyInhibin B Enzyme Immunoassay
Insulin Antibodies Validating
Insulin, Free Previously validated, currently inactive
Insulin,Total Chemiluminescence
Insulin-Like Growth Factor I (IGF-1) Chemiluminescent Immunometric
Insulin-Like Growth Factor I (IGF-1) Enzyme Immunoassay
Insulin-Like Growth Factor II (IGF-II) Previously validated, currently inactive
Insulin-Like Growth Factor Binding Protein 3, IGFBP3 Chemiluminescent Immunometric
Interferon Gamma Mulitplex Immunoassay
Intracellular Adhesion Molecule, sICAM Multiplex Immunoassay
Ionized Calcium Medica EasyLyte
IP-10/CXXL10 (Interferon gamma inducible protein 10 ) Enzyme Immunoassay (validating)
Iron, Total Colorimetric
JAK-2 Mutation PT-PCR (validating)
Jo-1 (histidyl t-RNA synthetase) Antibodies Previously validated, currently inactive
KIM-1, Kidney Injury Molecule-1 Enzyme Immunoassay
KRAS Mutation Real-time PCR
Lactate Enzymatic
Lactate Dehydrogenase, LD Enzymatic
Lactate Dehydrogenase, Urine Previously validated, currently inactive
Lactoferrin, Qualitative (Fecal Leukocytes) Chromatographic Immunoassay
Lactoferrin, Quantitative (Stool) Enzyme Immunoassay
Lactoferrin (Plasma) Previously validated, currently inactive
Lamotrigine Previously validated, currently inactive
LD isoenzymes Electrophoresis
LDL Direct Enzymatic
LDL Lipoparticle size Electrophoresis
Legionella pneumophilia Antibody Indirect Fluorescent Antibody
Legionella Urinary Antigen Immunochromatography
Leptin Enzyme Immunoassay
Lipase Colorimetric
Lipoprotein a Nephelometry
Lipoprotein Remnant - Apo B Previously validated, currently inactive
Lipoprotein Remnant - Cholesterol Previously validated, currently inactive
Lithium Ion Selective Electrode
LpA1 Previously validated, currently inactive
LpE Previously validated, currently inactive
Lp CIII, Lipoprotein C-III Electroimmunodiffusion
Lp-PLA2 , Lipoprotein-associated phospholipase A2 Enzyme Immunoassay
Luminex Bio-Rad Pro Human Cytokine 10-Plex Panel: IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12 (p70), IL-13, TNF alpha and IFN gamma
Multiplex Immunoassay (Consult Medical Affairs/Immunology)
Luminex LINCOplex Panel: Adiponectin, MMP-9, MPO, sE-selectin, sICAM-1 and sVCAM-1
Multiplex Immunoassay (Consult Medical Affairs/Immunology)
Lupus Anticoagulant (See Dilute Russell Viper Venom Test)
Luteinizing Hormone, LH Chemiluminescence
Magnesium Colorimetric
Magnesium, Urine Colorimetric
Mannitol Previously validated, currently inactive
MCP-1, Monocyte Chemotactic Protein 1 Enzyme Immunoassay
Covance Central Laboratory Services
*Special Collection DEC2011
Analytes MethodologyMethaqualone Confrimation Gas Chromotography/Mass Spectometry
Methaqualone Screen Immunoassay
Microalbumin, Quantitative Nephelometry
miRNA Isolation RNA Isolation
Mitochondrial Antibodies Immunofluorescence
MMP-1 ( see pro MMP-1) Previously validated, currently inactive
MMP-2 Previously validated, currently inactive
MMP-3 Previously validated, currently inactive
MMP-9 Multiplex Immunoassay
MMP-13 Previously validated, currently inactive
Mumps Antibody, IgG Enzyme Immunoassay
Mycobacteriology Consult Lab
Mycology Consult Lab
Mycoplasma culture Microbiology
Mycoplasma pneumoniae Antibody, IgG Indirect Fluorescent Antibody
Mycoplasma pneumoniae Antibody, IgM Indirect Fluorescent Antibody
Myeloperoxidase, MPO Multiplex Immunoassay
Myoglobin Nephelometry
Myoglobin, Urine Point of Care Instrument
NAT2 Genotyping Previously validated, currently inactive
Neisseria gonorrhoeae DNA Chemiluminescence
N-acetyl-B-D-glucosamidase, NAG Colorimetric
Neopterin Previously validated, currently inactive
NGAL (Neutrophil Gelatinase-associated lipocalin) Enzyme Immunoassay
Nitrites/Nitrates Previously validated, currently inactive
N-Telopeptides (NTx) ELISA
N-Telopeptides (NTx), Urine ELISA
NT-proBNP ECLIA - Electrochemiluminescence Immunoassay
NT-proBNP Chemiluminescent Immunometric
Osmolality Freezing Point Depression
Ostase (see Alkaline Phosphatase, Bone Specific)
Osteocalcin ECLIA - Electrochemiluminescence Immunoassay
Ova and Parasite Examination Light Microscopy
Oxalate Previously validated, currently inactive
Oxidized LDL ELISA
Paracetamol (Acetaminophen) Previously validated, currently inactive
Parathyroid Hormone, Intact Chemiluminescence
Parvovirus IgG Previously validated, currently inactive
Parvovirus IgM Previously validated, currently inactive
PBMC Purification, Peripheral Blood Mononuclear Cells Histopaque/Lymphoprep
Pentraxin 3 Enzyme Immunoassay (validating)
Pepsinogen I Enzyme Immunoassay
Pepsinogen II Enzyme Immunoassay
Phenobarbital Previously validated, currently inactive
Phenytoin, Free Previously validated, currently inactive
Phenothiazines Confirmation Gas Chromotography/Mass Spectometry
Phenothiazines Screen Immunoassay
Phenytoin Previously validated, currently inactive
Phospholipids, Total Previously validated, currently inactive
Covance Central Laboratory Services
*Special Collection DEC2011
Analytes MethodologyPhosphorus Colorimetric
Phosphorus, Urine Colorimetric
PI3K Mutation RealTime PCR
PINP, Aminoterminal Propeptide of Type I Procollagen ECLIA - Electrochemiluminescence
Placental Growth Factor, PLGF Enzyme Immunoassay
Plasma Renin Activity, PRA Radioimmunoassay
Plasmin Alpha 2-Antiplasmin Complex (PAPc) Previously validated, currently inactive
Plasminogen Activity Previously validated, currently inactive
Plasminogen Activator Inhibitor 1 Activity, PAI-1 Enzyme Immunoassay
Plasminogen Activator Inhibitor 1 Antigen (PAI-1 Ag) Previously validated, currently inactive
PMN Elastase Enzyme Immunoassay
Pneumococcal Capsular Polysaccharide Antibody, IgG Enzyme Immunoassay
Pneumococcal Urinary Antigen Immunochromatography
Potassium Ion Selective Electrode
Potassium, Urine Ion Selective Electrode
Prealbumin Nephelometry
Primidone Previously validated, currently inactive
Procalcitonin ECLIA - Electrochemiluminescence Immunoassay
Procollagen Type I, CICP Enzyme Immunoassay
Procollagen Type III, PIIINP Chemiluminescence (validating)
Progesterone Chemiluminescence
Proinsulin Radioimmunoassay
Prolactin (for pediatric studies) Chemiluminescence Immunometric
Pro MMP-1 Previously validated, currently inactive
Prostate Specific Antigen, PSA Chemiluminescence
Protein C Activity Clot Detection
Protein Electrophoresis Electrophoresis
Protein Electrophoresis, Urine Electrophoresis
Protein S Activity Clot Detection
Protein, Total Colorimetric
Protein, Total - Urine Colorimetric
Protein, Total - Urine Colorimetric
Prothrombin Time, PT Photometric Clot Detection
Prothrombin Fragment 1.2 Enzyme Immunoassay
Pseudo/Ephedrine Screen and Confirmation Thin-Layer Chromatography
Pulsed Field Gel Electrophoresis PFGE
Pyridinium Crosslinks, Pyrilinks Enzyme Immunoassay
Pyridinoline Previously validated, currently inactive
PYY, Total (Peptide YY) Previously validated, currently inactive
QuantiFERON-TB Enzyme Immunoassay
Quinidine Previously validated, currently inactive
Ranitidine Previously validated, currently inactive
RANKL1, Soluble Receptor Activator of Nuclear Factor KappaB Ligand Previously validated, currently inactive
Rapamycin Previously validated, currently inactive
Renin, Plasma Renin Activity, PRA Radioimmunoassay
Renin, Direct/Active Previously validated, currently inactive
Reticulocyte Count Flow Cytometry
*Special Collection DEC2011
Covance Central Laboratory Services
Analytes Methodology Reticulocyte Hemoglobin Content, CHr Light Scattering
Retinol Binding Protein Nephelometry
Rheumatoid Factor, RF Nephelometry
RLP (Remnant Lipoprotein) Cholesterol ELISA
RNA Integrity Agilent 2100
RNA Isolation, Blood RNA Isolation
RNA Isolation, Bone Marrow Validating
RNA Isolation, Tissue RNA Isolation
RNP Antibodies Fluorescent Microparticle Immunoassay
Rotovirus Group A Antigen Enzyme Immunoassay
RPR, Rapid Plasma Reagin Particle Agglutination
Rubella Antibody, IgG Chemiluminescence
Salicylate Previously validated, currently inactive
sCD30 Previously validated, currently inactive
SAA (Serum Amyloid A) on HDL by PEG Enzyme Immunoassay
Scl-70 (Scleroderma) Antibodies Previously validated, currently inactive
SDF-1/CXCL12 (Stromal cell-derived factor 1 alpha) Enzyme Immunoassay
Sex Hormone Binding Globulin, SHBG Chemiluminescent Immunometric
Shiga Toxins 1 & 2 Immunochromatography
Sm Antibodies Fluorescent Microparticle Immunoassay
Small Dense LDL Apolipoprotein B Immunoturbidimetry
Small Dense LDL Cholesterol Enzymatic Colorimetric
Smooth Muscle Antibodies Immunofluorescence
Sodium Ion Selective Electrode
Sodium, Urine Ion Selective Electrode
Soluble Thrombomodulin Previously validated, currently inactive
Soluble Transferrin Receptor Nephelometry
Soluble Tumor Necrosis Factor Receptor 1(sTNF RI) Previously validated, currently inactive
Soluble Tumor Necrosis Factor Receptor II (sTNF RII) Previously validated, currently inactive
SSA (Ro) Antibodies Fluorescent Microparticle Immunoassay
SSB (La) Antibodies Fluorescent Microparticle Immunoassay
T3, Total Chemiluminescence
T4, Total Chemiluminescence
TARC, Thymus and Activation-Regulated Chemokine Enzyme Immunoassay
Tetanus Antitoxoid Antibody, IgG Enzyme Immunoassay
Testosterone, Total Chemiluminescence
Testosterone, Total LC/MS/MS
Testosterone, Total (Low Sensitivity - LLOQ 2,00 ng/dl) LC/MS/MS
TGF Alpha (Transforming Growth Factor Alpha) Previously validated, currently inactive
TGF Beta 1 (Transforming Growth Factor Beta 1) Enzyme Immunoassay
TGF Beta 2 (Transforming Growth Factor Beta 2) Previously validated, currently inactive
Theophylline Chemiluminescence
Thrombin Time Clot Detection
Thrombin-Antithrombin III Complex ELISA
Thrombomodulin, Urine Previously validated, currently inactive
Thrombopoietin ELISA
Thyroglobulin Chemiluminescent Immunometric
Thyroglobulin Antibodies Chemiluminescent Immunometric
Covance Central Laboratory Services
*Special Collection DEC2011
Analytes Methodology Thyroid Stimulating Hormone, TSH Chemiluminescent
Thyroperoxidase (TPO) Antibodies Chemiluminescent Immunometric
Thyroxine Binding Globulin, TBG Previously validated, currently inactive
Tissue Inhibitors of Metalloproteinase I, TIMP-1 Previously validated, currently inactive
Tissue Plasminogen Activator (tPA) Antigen Previously validated, currently inactive
Tissue Transglutaminase IgA Previously validated, currently inactive
TNF-a, Tumor Necrosis Factor-alpha Enzyme Immunoassay
TNF-a, Tumor Necrosis Factor-alpha See Luminex Bio-Rad Pro Human Cytokine 10 Plex Panel
Topiramate Previously validated, currently inactive
Transferrin Nephelometry
Transforming Growth Factor Beta 1 (TGF Beta 1) Previously validated, currently inactive
Trichomonas Vaginalis Test Microbiology
Triglycerides Enzymatic
Troponin I Chemiluminescence
Troponin T ECL - Electrochemiluminescence
Troponin T, high sensitivity ECL-Electrochemiluminescence (validating)
Trypsin-like Immunoreactivity, TLI Radioimmunoassay
Tryptase Previously validated, currently inactive
T-Uptake Chemiluminescence
Unbound Iron Binding Capacity, UIBC Colorimetric
uPA, Urokinase-type Plasminogen Activator Enzyme Immunoassay
Urea Nitrogen, BUN Enzymatic
Urea Nitrogen, Urine Enzymatic
Ureaplasma Culture Microbiology
Uric Acid Colorimetric
Uric Acid, Urine Colorimetric
Valproic Acid Chemiluminescence
Valproic Acid, Free Previously validated, currently inactive
Vancomycin Previously validated, currently inactive
Varicella zoster IgG Antibody Chemiluminescence
(Soluble) Vascular Cell Adhesion Molecule I , sVCAM-1 Multiplex Immunoassay
Vascular Endothelial Growth Factor, VEGF ELISA
Vascular Endothelial Growth Factor Receptor I, VEGF-R1 Previously validated, currently inactive
Vascular Endothelial Growth Factor Receptor 2, VEGF-R2 Previously validated, currently inactive
Virology Consult Lab
Vitamin A and E High Performance Liquid Chromatography
Vitamin B12 Chemiluminescence
Vitamin D, 25-Hydroxyvitamin D Chemiluminescence
Vitamin D, 1,25-Dihydroxyvitamin D Radioimmunoassay
Vitamin K High Performance Liquid Chromatography
Warfarin Sensitivity (includes CYP2C9 and VKORC1 Genotyping) xTAG Mutation Detection
VKORC1 Genotyping (See Warfarin Sensitivity)
Von Willebrand Factor Antigen Previously validated, currently inactive
Zolpidem Previously validated, currently inactive
*Special Collection DEC2011
Covance Central Laboratory Services
Capabilities
Antibody development, antibody characterization & qualification, assaydevelopment
Assay development
Biacore
Capillary electrophoresis
Cell-based assays
Chemiluminescence
Chromatography - GC
Chromatography - GC/MS
Chromatography - HPLC
Chromatography - UPLC
Chromatography - HRMS
Chromatography - LC/MS
Chromatography - LC/MS/MS
Clinical pathology testing
Custom organic synthesis
ECG
ECG Digitography
ECL-A (Electrochemiluminescence assay)
ELISA - Enzyme Linked ImmunoSorbent Assay
Extraction techniques
FACS (Flow Cytometry)
Fluorescence Microscopy
FISH - Fluorescent In-Situ Hybridization
Genotyping - SNP analysis (Single-Nucleotide Polymorphisms)
Genotyping - Viral
Interphase FISH
Imaging
Immunoassays (other)
Immunochemistry
Immunofluorescence
Immunogenicity
IHC (Immunohistochemistry)ISH (In situ Hybridization)
In vitro ADME (Adsorption, Distribution, Metabolism and Excretion)
Immunofluorescence
IP/Western (ImmunoPrecipitation/Western Blot)
IRMA
KIRA
Microarray - DNA/RNA
Microarray - Tissue
Molecular assays
Multiplex immunoassays
New procedure development and validation
PCR/Sequencing
qRT-PCR (Quantitative Real Time PCR)
RT-PCR
Regulatory submission
Report and abstract preparation
RIA
RNA / DNA Isolation and Banking
Sample analysis
Sequencing
If you have any questions, please contact your Covance Account Executive using the information below.
THE AMERICAS +1-888-COVANCE (+1-888-268-2623) +1-609-419-2240
EUROPE/AFRICA +800-2682-2682 +44-1423-500888
ASIA PACIFIC +800-6568-3000 +65-6-5677333
EMAIL [email protected] WEB SITE www.covance.com
18
Additional Expertise
Small Molecule Large Molecule
Covance has extensive experience working with the following compounds. Currently these methods are inactive and may require additional time and/or fees to reactivate.
25-Desacetylrifampin
5-Fluorouracil
9-Hydroxyrisperidone
Alendronate
Atenolol
Cimetidine
Ciprofloxacin Hydrochloride
Cisapride
Cisplatin
Clarithromycin
Clonidine
Desmethyl Selegiline
Dihydrotestosterone
Diltiazem
Fentanyl
Fexofenadine
Fluconazole
Gemfibrozil
Griseofulvin
H2G
Haloperidol
Hydrochlorothiazide
Hydroxybutyrate, gamma
Ibuprofen
Ibuprofen (R-)
Ibuprofen (S-)
Ketoprofen
Ketoprofen (R-)
Ketoprofen (S-)
Levetiracetam
Lidocaine
Lovastatin
Lovastatin Acid
Menthol
Methadone (R-)
Methadone (S-)
Methyl Salicylate
Metoprolol
Mirtazapine
Modafinil (R-)
Modafinil (S-)
Modafinil acid (R-)
Modafinil acid (S-)
Modafinil sulfone
Naltrexol, beta
Naltrexone
Nifedipine
Nitrosamine, free
Nitrosamine, total
NNAL, free
NNAL, total
Norethindrone
Norfluoxetine
Norhydrocodone
Oxaliplatin
Oxycodone
Oxymorphone
Paracetamol
Prilocaine
Protriptyline
Ranitidine
Rapamycin
Rifampin
Risperidone
Salicylic Acid
Selegiline
Terfenadine
Theobromine
Trimethoprim
Adrenocorticotropic Hormone, (ACTH)
Anti-PEG antibodies
Bone-Specific Alkaline Phosphatase
Calcitonin
Chorionic Gonadotropin, beta subunit
Chorionic Gonadotropin, whole molecule
Creatine Kinase
Corticotropin Releasing Factor
C-Peptide
C-Reactive Protein
cTroponin I
cTroponin T
Cystatin C
Estradiol (E2)
Exendin
FABP3
Factor IX
Factor VII
Factor VIIa
Factor VIII
Factor VIII inh
Ferritin
Growth Hormone
Hepatocyte Growth Factor
IgA
IgE
IGF-BP3
IGF-I
IgG, total
IgG1
IgG2
IgG3
IgG4
IgM
Insulin (RIA and ELISA)
Insulin Glargine
Insulin LisPro
Insulin, TOSOH
Kim-1
Lysozyme
Myl3
Neopterin
N-Telopeptide (Procollogen N-Term)
Osteocalcin
Osteoprotegrin
P1NP
Parathyroid Hormone
Parvalbumin
Prolactin
Pyrilinks (Pyridinium)
Pyrilinks-D (Deoxypyridinium)
Sema4D
sRANKL
Thyroid Stimulating Hormone (TSH)
Thyroxine (T4)
TIMP-1
Tobramycin
TRAP-5b
Triiodothryonine (T3), total