BioNordika Finland Oy • tel 0207 410 270 • info@bionordika.fi • www.bionordika.fi

May - July 2020

Issue 2

FEATURING:

• New supplier Eppendorf• New products for protein work• Help for planning your experiments

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Let's talk ...our new suppliers and new product manager

Welcome back, Mirja!We are happy to welcome Mirja Heinonen back to BioNordika as a product manager.

Mirja will strenghten our molecular biology team with her positive attitude and strong knowledge background.

She can be contacted at: mirja.heinonen@bionordika.fi. phone 020 7410 279

is also one of our new supplier!Eppendorf is a leading life science company that develops and sells instruments, consumables and services for laboratories worldwide

• LAB CONSUMABLES Tubes, tips, plates, stem cell cultureware, cell culture consumables, cell imaging consumables, cuvettes, and sealing options. • MANUAL LIQUID HANDLING Manual pipetting and dispensing, pipette tips, automated pipetting, and accessories. • TEMPERATURE CONTROL AND MIXING Thermoblocks with or without mixing options. • PCR Cyclers, PCR accessories, PCR consumables, and realtime PCR consumables. • CO2-INCUBATORS

We are happy to introduce these suppliers:

SPECIAL BUNDLE PRICE

2 551 EUR2230 000.032 Centrifuge 5425 G : with rotary knobs, Rotor FA-24x2, aerosol-tight for 24 × 1.5/2.0 mL tubes, incl. aerosol-tight rotor lid, incl. DNA LoBind Tubes, 1.5 mL, PCR clean, colorless, 250 tubes (5 bags × 50 tubes)

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Let's talk ...about centrifugation and pipetting

EasyPet 3® Experience a new dimension of electronic pipetting

• Intuitive and convenient speed adjustment simply done with the tips of your fingers• Lightweight, well-balanced and ergonomic design• Lithium polymer rechargeable battery offers long cordless runtime• Smooth setting of pump speed• Operation while recharging is possible• Vibrant backlit LEDs provide optical feedback of the remaining battery life• Autoclavable pipette adapter for sterile applications• Quick release of aspirating cone for easy exchange of membrane filters• Pipette controller for use with pipettes from 0.1  –  100 mL

Ask more from Sari!Sari Nousiainen

Sari.nousiainen@bionordika.fi Tel. 020 7410 280

A good combination; EasyPet3 and Greiner's serological pipettes

-10%Introduction

offer

Valid until 31.7.2020 with promo code BN-SPRING20

Your reliable partners 5425!• No sample contamination as air is blown from the outside along the hermetically tight chamber (passive cooling concept)• Quiet as a whisper - 12 minutes of operation at an average 45 dB(A) - without compromising temperature stability, max g-force 21,300 x g• Rotors available for 1.5 / 2.0 mL, 5 mL, and up to 96 x 0.2 mL• Soft-touch, one-finger closure for ergonomic lid locking

LoBind Tubes are useful for low DNA sample concentrations as well as difficult preparations such as NGS

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Valid until 31.7.2020 with promo code BN-SPRING20

Let's talk ...about DNA/RNA quantification

AccuGreen kits for Qubit fluorometerBiotium offers two DNA quantification kits which are pre-programmed assays on with Qubit fluorometer:

DNA quantification

Talk to Juho!Juho Niva Juho.niva@bionordika.fi Tel. 020 7410 276

AccuGreen™ Broad Range (Cat# BT31069)• Linear range from 2 to 1000 ng dsDNA• Direct replacement for the Qubit® dsDNA BR Assay Kit

AccuGreen™ High Sensitivity (Cat# BT31066)• Linear range from 0.1 to 100 ng dsDNA • Direct replacement for the Qubit® dsDNA HS Assay Kit

DNA quantification with a plate readerFor bigger sample amounts there are four different DNA quantification kits available for fluorescent plate readers:

AccuBlue® Broad Range (Ca# BT31007)• Linear range from 2 to 2000 ng ds DNA• Best assay for high-concentration DNA samples (e.g. minipreps and maxipreps)

AccuClear® Ultra High Sensitivity (Cat# BT31028, BT31029)• Widest linear range from 0.03 to 250 ng dsDNA! • Best choice for most typical DNA samples

AccuBlue® High Sensitivity (Cat# BT31006)• Linear range from 0.2 to 100 ng ds DNA• Non-toxic and non-mutagenic membrane-impermeant dye (safer handling and easy disposal)

AccuBlue® NextGen (Cat# 31060)• Linear range from 5 to 3000 pg dsDNA• Most sensitive and accurate dsDNA quantification reagent on the market!• Ideal for quantitation of precious samples

RNA quantificationAccuBlue RNA quantification kitAccuBlue RNA quantification kit can be used with plate readers and Qubit fluoro-meter. The kit offers the broadest range for RNA quantification and is highly specific for RNA!

• Highly specific for RNA over dsDNA• Superior sensitivity: down to 5 ng on plate reader• Linear range of 5-1000 ng RNA• Optimized for use in the Qubit® preprogrammed RNA Broad Range Assay

10% off from all DNA/RNA quantification kits

• Seamless cloning in as little as 15 minutes• Either for single stranded oligos or DNA fragments of different sizes (up to 20 kb) and overlapping regions (15 – 80 bp)• No PCR-cleanup step needed• Free tool for designing primers available• Available with or without competent cells

Share your thoughts with Mirja!

Mirja HeinonenMirja.heinonen@bionordika.fi

Tel. 020 7410 279

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CELLEDYRKNING

Valid until 31.7.2020 with promo code BN-SPRING20

Let's talk ...about protein expertise

NEBuilder for fast cloning

Let us give you an example:

What do we have for the most common type of protein purification, His-tag purification?

Find the best purification beads, columns & resins

RNA quantification

10% off from all DNA/RNA quantification kits

Over 40 years of protein expression & purificationIn 1980 first recombinant enzymes by NEB were offered for sale and to date NEB's expertise has enabled the commercialization of over 550 recombinant enzymes. You can enjoy the help of these enzymes either as stand-alone versions or as handy kits.

• Cell-free E.coli Protein Expression System• All the components for protein synthesis except the target template DNA• High yields (~0,5 mg/ml) of protein in ~2 - 4 hours• Protein targets ranging from 17 to 230 kDa• For high-throughput expression applications and toxic protein expression (protein engineering, mutagenesis studies and enzyme screening)

NEBExpress for fast protein expression

NEBExpress® Ni-NTA magnetic

beads for scalable purification NEBExpress®

Ni Resin for purification with

your own columns

NEBExpress® Ni Spin Columns in ready-to-use

formatMagnetic separation racks

for effective purification

TEV protease for His-tag

removal

Request a

free sample

Request a

free sample

-10%on all

NEBuilder products

NEW!

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Let's talk ...about protein purification

Protein Ark HiFliQ FPLC columnsHiFLiQ FPLC columns are compatible with all common chromatography instruments (including GE Healthcare ÄKTÄ™). Protein Ark HiFLiQ columns are pre-packed with Nickel-NTA, Cobalt-NTA, heparin or glutathione agarose for the purification of most common tag and non-tag proteins.

Protein Ark also offers Protein A & G HiFliQ FPLC columns, and ion exchange Q-type & S-type HiFliQ FPLC columns pre-packed and ready to use for simple, one-step and rapid antibody purification.

Talk to Juho!Juho Niva Juho.niva@bionordika.fi Tel. 020 7410 276

Resins and empty FPLC columnsDon’t need pre-packed FPLC columns? No problem! Protein Ark has also loose resins and empty FPLC columns available:

Proteus X-spinner 2.5 ultrafiltration Exceptional ultrafiltration performance for viscous samples, sticky and hydrophobic proteins, membrane proteins.

• Performance: 2.5 ml sample filtered in 30 min• Filter: CTA & PES (5 kDa, 10 kDa, 20 kDa, 100 kDa, 300 kDa MWCO) • Feature: Unique reverse design prevents membrane caking and protein losses

Protein Ark offers empty 1 ml, 5 ml, 10 ml and 20 ml FPLC chromatography columns at an affordable price. Both ends of the FliQ columns have 10-32 UNF

threads which fits all common chromatography instruments such as ÄKTA™.

Valid until 31.7.2020 with promo code BN-SPRING20

FastBack resins Super resins

• Ideal for fast purification • Mean bead size: 90µm • For Nickel, Cobalt, Protein A & G, Q-EX and S-IEX IMAC chemistries • 10 ml, 20 ml and 100 ml sizes

• High performance resin • Mean bead size: 35µM • Ni-NTA, Co-NTA, Heparin and Glutathione options available • 1 ml, 10 ml, 20 ml and 100 ml sizes

-10%HiFliQ FPLCColumns &

resins

Request

a sample

7

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Let's talk ...about protein-related services

Valid until 30.6.2020 with promo code BN-SPRING20

Whether you are interested in crystallography, protein-protein interactions, antibody production or activity assays, Eurogentec proposes a protein expression service to advance your research needs.

Don't want to do protein production on your own?

Share your thoughts with Mijra!

Mirja HeinonenMirja.heinonen@bionordika.fi

Tel. 020 7410 279

Custom recombinant protein service:• Expression in E. coli• Expression in HEK or CHO cells• Choice of different protein tags• Choice of purification levels from standard purity (85 %) to high purity (> 90 %)

Our supplier Eurogentec has just upgraded their peptide production facilities in Belgium. What does this mean to you?

By merging the latest in peptide technology with a highly-experienced team, the production costs go down and peptides are available in more attractive prices with technical support just around the corner. BioNordika is happy to help you though any project you may have!

Don't forget our catalogue options either! They include for instance several proteins in the field of neurosciences and cancer research. Ask for a list!

Cutting-edge peptide lab near you!

Valid until 31.7.2020 with promo code BN-SPRING20

Talk to Mirja!Mirja HeinonenMirja.heinonen@bionordika.fiTel. 020 7410 279

Valid until 31.7.2020 with promo code BN-SPRING208

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Let's talk ...about sample preparation

• Rotation during sonication Consistent shearing

• Adaptive cavitation technology Random and gentle

• Temperature control Preserve biological samples

• Closed system No cross-contamination and aerosol-formation

• Fast parallel processing Save time and money

• Fully validated and inexpensive tubes Create quality results and save money

• 5-year guarantee Long-term promise of quality

The new Bioruptor Pico is here!

Request a

demo!

NEW!

Pippins you know, but what does SageHLS?Pippin products are well known amongst researchers performing size selection for NGS. The newest innovation from Sage Science takes size selection even further: May we present to you, SageHLS, one-stop sample prep for long-range genomics!

1) Extract DNA from cells, nuclei or spheroplasts by loading them into the cassette

2) Add cleavase or Cas9 and gRNA

3) Pulsed-field electrophoresis fragments DNA based on the application

4) You are ready! Now you have a certain size of DNA extracted directly from the cells and even from desired regions (f.ex. only BRCA1 and BRCA2 gene regions).

Luna cell counters

• Detect accurately total/live/dead cells• Microscopy optics and image analysis software• Onboard computer and optional printer• Cell-size gating• Exceptional accuracy in cell clump declustering• Professional pdf-report• Results in minutes• Minimal running costs• Ecological reusable slides available

Viability staining options• For bright-field counting: Trypan blue or safer Erythrosin B• For fluorescence counting: Acridine orange/Propidium iodide (eukaryotic cells) or Fluorescein diacetate/Propidium iodide (yeast cells)

Valid until 31.7.2020 with promo code BN-SPRING20 9

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Let's talk ...about the best ways to count various cell types

NEW!

Automated cell counting with Luna is quick, easy and reliable

STAIN LOAD COUNT REVIEW & EXPORT

Luna FX7

soonavailable!

Brightfield counters• Count cells with or without staining• Viability stains trypan blue or Erythrosin B

Fluorescence counters• Dual fluorescence optics and bright-field optics• Optimized for primary cells, PBMCs• Accurate live/dead cell counting without limitation of cell types• Acridine orange/propidium iodide staining, GFP• Counting of both nucleated and non-nucleated cells

Microbial counters• Both fluorescence and bright field counting• For yeast cells used in food and drink industry• Bacterial cell counter for total amount of cells and viable cells

Valid until 31.7.2020 with promo code BN-SPRING2010

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Let's talk ...about transfections and genome editing

Let me help!Hanna Laiho Hanna.laiho@bionordika.fi Tel. 020 7410 280

Transfect hard-to-transfect cells efficiently and gently with Nucleofector• Non-viral, reproducible, highly efficient transfection method• Retains viability and cell functionality• Optimized protocols for more than 650 different cell types• Easy scaleability of the cell number and the sample throughput• Deliver DNA, RNA, oligonucleotides, ribonucleoproteins, PNA, peptides or proteins

CRISPR/Cas9 with Nucleofector• All genome editing tools require co-transfer of several substrates into the cell type of interest for successful modification of genomic DNA • High transfection efficiencies for a broad range of cell types, including iPSCs and immune cells: T-cells, NK and dendritic cells • Efficient co-transfection of various substrates

-15%Nucleofectorinstruments

More tools for genome editing• EnGen Spy Cas9 NLS: ideal for ribonucleoprotein complexes, with nuclear localization signal

• EnGen sgRNA Synthesis Kit: for rapid generation of sgRNA from your target-specific ssDNA template

• EnGen Mutation Detection Kit: for detecting targeting efficiency with a simple PCR + T7-Endonuclease- digestion based protocol • CRISPR/Cas9 vectors and Gene Knockout Kits from Origene

10% OFF from Vector TrueVIEW products!

Valid until 31.7.2020 with promo code BN-SPRING20 11

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Let's talk ...about immunofluorescence

NEW! VECTASHIELD PLUS Mounting Medium (Cat# H-1900, H-2000) • New superior non-setting formulation• No inherent toning or background• Superior signal retention across the whole spectrum • Specimen can be viewed after 1 hour of mounting • Available with or without DAPI counterstain, in 2 ml and 10 ml packages

VECTASHIELD Antifade Mounting Medium (Cat# H-1000, H-1200, H-1300) • The most popular mounting media• Non-hardening• Available with or without DAPI andpropidium iodide (PI)

VECTASHIELD HardSet Mounting Medium (Cat# H-1400, H-1500, H-1600)• Hardens at RT in less than 20 mins• No need to seal the slides for longer storage• Available with or without DAPI and TRITC-Phalloidin

VECTASHIELD Vibrance Mounting Medium (CAT# H-1700, H-1800)• Superior antifade properties across the whole spectrum • View sections after 1 hour of mounting• Slides can be stored in RT for several weeks• Minimal bubble formation• Available with or without DAPI counterstain, and in 2 ml and 10 ml packages

VECTASHIELD antifade mounting mediaComplete your IF workflow with the most widely referenced antifade mounting media available from Vector Laboratories (over 60000 references).

Talk to Juho!Juho Niva

Juho.niva@bionordika.fi Tel. 020 7410 276

Autofluorescence quenching kitsSimple, fast and effective way to get rid off non-lipofuscin mediated autofluores-cence from your tissue or cell sections. These kits quenches the autofluorescence from multiple sources e.g. red blood cells, structural elements (collagen, elastin) and aldehyde fixation.

• Fast and easy-to-use, one-step (5 min) method• Compatible with wide selection of fluorophoresand mounting medias• For standard epifluorescence and confocal laser microscopes• Includes VECTASHIELD Vibrance mounting mediawith or without DAPI counterstain• NEW! 3ml sample size available (Cat# SP-8403)

12%OFF!

Need to seal the wet-mounted slides? You can seal your coverslip for longer storage with CoverGrip™ sealant from Biotium• No ingredients that could affect the specimen fluorescence • Contains d-limonene, a natural, environmentally-friendy solvent• Available in 15 ml with a brush-cap and 100 ml dropper-cap refill sizes

BioNordika Finland Oy • Kutomotie 18, 00380 Helsinki tel 0207 410 270 • info@bionordika.fi • www.bionordika.fi

Promo code: BN-SPRING20Note! The promotional code must be referenced when ordering to receive the

discounts and special prices. The offers are listed ex. VAT and shipping charges, and are valid until 31.07.2020. The offers cannot be combined with other offers

or agreements. We reserve the right for typos.

Let's talk

Contact us for any issues We are all happy to help you!

HANNA LAIHO Product Manager, Cellbiology hanna.laiho@bionordika.fi tel. 020 7410 280

SARI NOUSIAINEN CEO sari.nousiainen@bionordika.fi tel. 020 7410 280

JUHO NIVA Product Manager, Antibodies juho.niva@bionordika.fi tel. 020 7410 276

MIRJA HEINONEN Product Manager, Molecular biology mirja.heinonen@bionordika.fi tel. 020 7410 279

TOMI LEUNG Customer service tomi.leung@bionordika.fi tel. 020 7410 270

PIRKKO TUOMISALO Finance Manager pirkko.tuomisalo@bionordika.fi tel. 020 7410 272

ELINA LINNA Customer service eloina.linna@bionordika.fi tel. 020 7410 270