final powerpoint parasites
TRANSCRIPT
LAB REVIEW
PARASITES&
BLOOD CELLS
Band Neutrophil
Red Blood Cells
Lymphocyte
Eosinophil
Platelets
Basophil (in horses it is blue)
Monocyte
Segmented Neutrophil
Tapeworm
Hookworm
Heartworm filaria
Coccidia
Whipworm
Mites
Giardia
FLEA
Mite
Babesia Canis
Sucking Lice…………..Biting Lice
American Dog Tick….……..Deer Tick
Lone Star Tick………Rocky Mtn Wood Tick
Dirofilaria immitum….dipeteloma reconditum
Hemobartonella???
Avian Blood
Warble or Cuterebra (fly larvae)
Anaplasma Marginale
Lung Worm
paragonimus kellicotti
Lung Fluke
Eucoleus boehmi
Urine
Physical Exam:
Smell
Color
Turbidity
Foam
Urine
Chemical Exam:pH
Bilirubin
Protein
Occult Blood
Glucose
Ketones
Specific gravity
Nitrites
WBCs
Heartworm Test
Buffy Coat method (hematocrit test)
Modified Knott’s technique
Filter membrane technique/Di-fil
Blood Smear
SNAP: Antigen testing does not detect the actual parasite but the host’s response to the
disease
Steps For Modified Knotts
Mix 1 ml of blood w/ 9ml of 2% formalin in centrifuge
Centrifuge tube at 1300 to 1500 rpm for 5 minutes
Pour off liquid supernatant, leaving sediment at bottom of tube
Add 2-3 drops of stain to sediment. Using pipette, mix sediment w/stain
Place drop of this mixture on glass slide. Apply coverslip. Examine for microfilariae using 10X objective.
Filter MembraneMix 1 ml of blood w/9 ml of lysing solution in 15 ml test tube
Place filter membrane in filter holder, close tightly, do not pinch seal in threads of holder
Connect syringe to filter holder and gently empty through filter holder
Fill syringe with water and repeat above step
Open holder, use thumb forceps to remove filter membrane
Place filter membrane on a clean microscope slide
Add one drop of stain, cover with coverslip, examine for microfilariae
Stages of a Parasites Life Cycle
• 1. oocyst
• 2. Larvae
• 3. nymph
• 4. Pupa
• 5. Adult