Gayam, Glydenne Glaire Gayam MT 34/340 Bacteriology BS Medical Te chnology 3A Staphylococcus epidermidis Cultural Characteristics Small, white or yellow (usually non-igmented ! gray or grayish white", aro#imately $%&-4 mm in diameter, S-tye colonies% Growth in 'roth is irst tur'id, later 'ecoming clear wi th a i ne or sl ig ht ly mucoid de osi t% )aculatati *e ly anaero'ic, otimal temerature $+-3. (growth range &-4&."% Grow on mediaTryticase Soy Agar 1 &2 SheeBlood, .haman (selecti*e medium with & g/a.l and mannitol", Mueller-5inton agar% 6n &2 She eBl ood Aga r, it ae ars smal l to medium7 oa8ue, gray-white colonies7 most colonies non-hemo lytic7 slime-roducing strains are e#treme ly stic9y and adhere to agar surace% Biochemical Characteristics :osi ti *e results o r nitrate reduction, al9aline hos hat ase, arg inine dihy drolase, urease, acetoin roduction, catalase, acid roduction rommannose and sucrose% egati*e results or starch hydrolysis, o#idase, growth og (54"$S ;4, coa gula se-ra''i t lasma, cluming act or, orn ithi ne dec ar'o #yl ase, deo# yri 'on ucle ase agar, heat-sta'le nuclease, 'eta-glucuronidase, 'eta- ga lactosidase, acid r od ucti on r omer yt hritol, ery thr ose, genti o' ios e, inosi tol, ly# ose, sor'ose, taga tose , treh alos e, mann itol , #yli tol, raino se, ara' inos e, cell o'io se, uc ose and salicin% <aria'le results or hya luronidase, 'eta -gl ucosidas e, acid roduction romlactose, galactose, mele=itose, turanose and ri'ose% )or 'ioche mica l test coagu lase , ther e is no clot ting which means it>s negat i*e orcoagulase% ?illey, @%M% et%al ($00"% Prescott, Harley, and Klein’s Microbiology (7th edition).McGraw-5ill% Mahon, .%% et% al ($0&"% Tetboo! o" #iagnostic Microbiology ($th edition)% Saunders Clse*ier% Staphylococcus epidermidis% htt//www %tgwD+%net /Stahylococcus/eid ermidis%html Coagulase-nega tive staphylococc i (CoNS) growing on sheep blood agar, revealing non hemol tic , wh ite cr eam col oni es.
