flow cytometrypresentation
TRANSCRIPT
![Page 1: Flow cytometrypresentation](https://reader036.vdocuments.net/reader036/viewer/2022070315/5550856ab4c905a85c8b4912/html5/thumbnails/1.jpg)
TEACHER GUIDE SHEETClick to view
POWERPOINT ACTIVITY
![Page 2: Flow cytometrypresentation](https://reader036.vdocuments.net/reader036/viewer/2022070315/5550856ab4c905a85c8b4912/html5/thumbnails/2.jpg)
What cells are in there?
How can we use Flow Cytometry to quantify cells?
![Page 3: Flow cytometrypresentation](https://reader036.vdocuments.net/reader036/viewer/2022070315/5550856ab4c905a85c8b4912/html5/thumbnails/3.jpg)
What is flow Cytometry?
Process for quantifying cells
•Measures different property of cells
•Able to categorize and quantify
•Even able to separate out subpopulations of cells
![Page 4: Flow cytometrypresentation](https://reader036.vdocuments.net/reader036/viewer/2022070315/5550856ab4c905a85c8b4912/html5/thumbnails/4.jpg)
• Clinical
•Research
Dr. Allan MCB Harvard
![Page 5: Flow cytometrypresentation](https://reader036.vdocuments.net/reader036/viewer/2022070315/5550856ab4c905a85c8b4912/html5/thumbnails/5.jpg)
NeutrophilsLymphocytesMonocytesEosinophilsBasophils
White Blood Cells, Platelets (stained purple), a T-Lymphocyte white cell (stained green), and a Monocyte white cell (stained gold) as seen through a scanning electron microscope. ©2000 Dennis Kunkel, Ph.D.Image Source: http://www.mybloodyourblood.org/biology_white.htm
![Page 6: Flow cytometrypresentation](https://reader036.vdocuments.net/reader036/viewer/2022070315/5550856ab4c905a85c8b4912/html5/thumbnails/6.jpg)
How could you separate your sample of white blood cells into subpopulations?
What characteristics of the cell could you use?How about if they had no color?
![Page 7: Flow cytometrypresentation](https://reader036.vdocuments.net/reader036/viewer/2022070315/5550856ab4c905a85c8b4912/html5/thumbnails/7.jpg)
Standard Differentials
1. Draw sample from patient
2. Smear blood on slide
3. Stain with differential WRIGHT stain
4. Count (at least 100) http://www.rnceus.com/cbc/cbcwbc.html
![Page 8: Flow cytometrypresentation](https://reader036.vdocuments.net/reader036/viewer/2022070315/5550856ab4c905a85c8b4912/html5/thumbnails/8.jpg)
Why do we need technology?
![Page 9: Flow cytometrypresentation](https://reader036.vdocuments.net/reader036/viewer/2022070315/5550856ab4c905a85c8b4912/html5/thumbnails/9.jpg)
How it works1. Draw cells, with excess fluid, from test tube into machine.
2. Cells pass in single file past laser.
3. Laser hits cell and light is scattered.
4. Photomultiplier multiplies light intensity and a light sensor measures the amount of light and scatter pattern.
5. Based on cell characteristics (size and shape), the computer categorizes and quantifies the cells.
Click to learn more
![Page 10: Flow cytometrypresentation](https://reader036.vdocuments.net/reader036/viewer/2022070315/5550856ab4c905a85c8b4912/html5/thumbnails/10.jpg)
Create a table to record data for your white blood cell differential.
** Remember you will need to record the number of each cell in your sample.
NeutrophilsLymphocytesMonocytesEosinophilsBasophils
![Page 11: Flow cytometrypresentation](https://reader036.vdocuments.net/reader036/viewer/2022070315/5550856ab4c905a85c8b4912/html5/thumbnails/11.jpg)
Use the following key to categorize your sample of White Blood Cells.
What is the percent break up of your white blood cell differential?
Candy Cell
Orange Runt Neutrophil
Green Runt Monocyte
Yellow Runt Basophil
Pink Runt Eosinophil
White Nerds Lymphocyte
![Page 12: Flow cytometrypresentation](https://reader036.vdocuments.net/reader036/viewer/2022070315/5550856ab4c905a85c8b4912/html5/thumbnails/12.jpg)
What can this differential tell you?
http://www.bioteach.ubc.ca/MolecularBiology/FlowCytometry/
See real Data
![Page 13: Flow cytometrypresentation](https://reader036.vdocuments.net/reader036/viewer/2022070315/5550856ab4c905a85c8b4912/html5/thumbnails/13.jpg)
Let’s take a closer look.
How can we differentiate T cells?
http://www.flow-cytometry.de/
![Page 14: Flow cytometrypresentation](https://reader036.vdocuments.net/reader036/viewer/2022070315/5550856ab4c905a85c8b4912/html5/thumbnails/14.jpg)
Create a table to categorize and count your T-cell subpopulation into CD4+ Helper T cells and CD8+ Cytotoxic T cells.
Purple Nerds= CD8+ Cytotoxic T cells
Pink Nerds += CD4+ Helper T cells
Categorize and count your T cell population
![Page 15: Flow cytometrypresentation](https://reader036.vdocuments.net/reader036/viewer/2022070315/5550856ab4c905a85c8b4912/html5/thumbnails/15.jpg)
What can the results tell you?
See real Data
![Page 16: Flow cytometrypresentation](https://reader036.vdocuments.net/reader036/viewer/2022070315/5550856ab4c905a85c8b4912/html5/thumbnails/16.jpg)
What other applications are there for the technology of flow cytometry?
Use the internet to explain 1 other application of the flow cytometer. E sure to document your resource.
![Page 17: Flow cytometrypresentation](https://reader036.vdocuments.net/reader036/viewer/2022070315/5550856ab4c905a85c8b4912/html5/thumbnails/17.jpg)
http://courses.dce.harvard.edu/-bios169c/resourcemain.html maintained by Dr. Jeffrey Lyczak
Resources