fogale. broschure biomass
DESCRIPTION
Broschure BiomassTRANSCRIPT
CELL CULTURE & FERMENTATION
Driving your bioreactor toward the future
FOGALE nanotech
Access incredible benefitswith the FOGALE Biomass System
viable cell density for yeast, bacterial, fungal, animal and plant cells.
It is suitable for the measurement of both very low and very high cell concentrations in complex media.
With additional software programs, it can be transformed into a full Biomass Analyzers.
For more detailed information: [email protected] / Tel. + 33 466 620 555, or visit our website at www.fogalebiotech.com
Online Biomass monitoring
Measures in real time and records all growthMonitors cell density in the culture mediaGreatly decreases contamination risks during samplingReduces the need for intensive lab analysis & eliminates human error
Biomass control has never been so easy !
An ideal instrument for closed loop control in batch, fed-batch and perfusionKeeping your cell density constant or optimizing your feed rate and timing is now very convenient
FOGALE Nanotech, knownas the worldwide reference for
viable biomass monitoringbased on capacitance, provides
specific standard & integrated solutions.
Wherever your business is located, our worldwide sup-port provides the service you deserve.Our team of experts will help you set up new systemsand will provide full-scope customer service.
Our sensors and systems, our experience and know-how, our professionalism and commitment are the gua-rantee of your entire satisfaction.
For more information about our products,please call us at +33 (0) 466 620 555or visit our website www.fogalebiotech.com
and all the advantages you wantavailable in a single model...
Stable and sensitivein all culture conditions
Not limitedby media conductivity
Modular designfrom 1 to 4 probes on the same control unit
Compact designprobes and pre-amplifiers fit all vessel sizes
User friendlyquick installation and easy handling
Frequency scanning and advanced softwaressystem upgradable at any time
...ALL for the best priceDistributor
Driving your bioreactor toward the future
Expertise and Performance
Optimize yield and shorten process development Increase control of your culture during scale-up Improve the level and quality of and traceabilityin production
Europe/Asia: FOGALE nanotech, France +33 466 620 555 - [email protected]
USA: FOGALE nanotech Inc, Los Angeles,CA / (310) 348 2974 - [email protected]
biomass-system2.indd 1 24/09/07 22:00:15
CELL CULTURE & FERMENTATION
Driving your bioreactor toward the future
FOGALE nanotech
Access incredible benefitswith the FOGALE Biomass System
viable cell density for yeast, bacterial, fungal, animal and plant cells.
It is suitable for the measurement of both very low and very high cell concentrations in complex media.
With additional software programs, it can be transformed into a full Biomass Analyzers.
For more detailed information: [email protected] / Tel. + 33 466 620 555, or visit our website at www.fogalebiotech.com
Online Biomass monitoring
Measures in real time and records all growthMonitors cell density in the culture mediaGreatly decreases contamination risks during samplingReduces the need for intensive lab analysis & eliminates human error
Biomass control has never been so easy !
An ideal instrument for closed loop control in batch, fed-batch and perfusionKeeping your cell density constant or optimizing your feed rate and timing is now very convenient
FOGALE Nanotech, knownas the worldwide reference for
viable biomass monitoringbased on capacitance, provides
specific standard & integrated solutions.
Wherever your business is located, our worldwide sup-port provides the service you deserve.Our team of experts will help you set up new systemsand will provide full-scope customer service.
Our sensors and systems, our experience and know-how, our professionalism and commitment are the gua-rantee of your entire satisfaction.
For more information about our products,please call us at +33 (0) 466 620 555or visit our website www.fogalebiotech.com
and all the advantages you wantavailable in a single model...
Stable and sensitivein all culture conditions
Not limitedby media conductivity
Modular designfrom 1 to 4 probes on the same control unit
Compact designprobes and pre-amplifiers fit all vessel sizes
User friendlyquick installation and easy handling
Frequency scanning and advanced softwaressystem upgradable at any time
...ALL for the best priceDistributor
Driving your bioreactor toward the future
Expertise and Performance
Optimize yield and shorten process development Increase control of your culture during scale-up Improve the level and quality of and traceabilityin production
Europe/Asia: FOGALE nanotech, France +33 466 620 555 - [email protected]
USA: FOGALE nanotech Inc, Los Angeles,CA / (310) 348 2974 - [email protected]
biomass-system2.indd 1 24/09/07 22:00:15
CELL CULTURE & FERMENTATION
Driving your bioreactor toward the future
FOGALE nanotech
Access incredible benefitswith the FOGALE Biomass System
viable cell density for yeast, bacterial, fungal, animal and plant cells.
It is suitable for the measurement of both very low and very high cell concentrations in complex media.
With additional software programs, it can be transformed into a full Biomass Analyzers.
For more detailed information: [email protected] / Tel. + 33 466 620 555, or visit our website at www.fogalebiotech.com
Online Biomass monitoring
Measures in real time and records all growthMonitors cell density in the culture mediaGreatly decreases contamination risks during samplingReduces the need for intensive lab analysis & eliminates human error
Biomass control has never been so easy !
An ideal instrument for closed loop control in batch, fed-batch and perfusionKeeping your cell density constant or optimizing your feed rate and timing is now very convenient
FOGALE Nanotech, knownas the worldwide reference for
viable biomass monitoringbased on capacitance, provides
specific standard & integrated solutions.
Wherever your business is located, our worldwide sup-port provides the service you deserve.Our team of experts will help you set up new systemsand will provide full-scope customer service.
Our sensors and systems, our experience and know-how, our professionalism and commitment are the gua-rantee of your entire satisfaction.
For more information about our products,please call us at +33 (0) 466 620 555or visit our website www.fogalebiotech.com
and all the advantages you wantavailable in a single model...
Stable and sensitivein all culture conditions
Not limitedby media conductivity
Modular designfrom 1 to 4 probes on the same control unit
Compact designprobes and pre-amplifiers fit all vessel sizes
User friendlyquick installation and easy handling
Frequency scanning and advanced softwaressystem upgradable at any time
...ALL for the best priceDistributor
Driving your bioreactor toward the future
Expertise and Performance
Optimize yield and shorten process development Increase control of your culture during scale-up Improve the level and quality of and traceabilityin production
Europe/Asia: FOGALE nanotech, France +33 466 620 555 - [email protected]
USA: FOGALE nanotech Inc, Los Angeles,CA / (310) 348 2974 - [email protected]
biomass-system2.indd 1 24/09/07 22:00:15
Driving your bioreactor toward the future
For more detailed information: [email protected] / Tel. + 33 466 620 555, or visit our website at www.fogalebiotech.com
Benefits for cell cultureFrom process development to cGMP environments, the Biomass System is the choice of many cell culture engineers to optimize their bioreactor operation strategy.
Outstanding performance:
- at lab level to understand the process and/or optimize the control strategy
- for large-scale manufacturing, including the identification of process deviation, of optimal harvest time and/or PAT application
- in animal cell production, whether in suspension or attached to microcarriers
- with all of the following cell lines: CHO, PER.C6, Vero, BHK, Hybbridoma, HEK 293, NS0, Hela, Sf9, Sp2/0
ApplicationsSf9 growth and infection process by a baculovirus (BEVS)
CHO fed-batch cultivation
Benefits for fermentation
- Cell growth on glycerol- Induction time (T1)- Fed-batch on methanol- Harvest time (T2)
0,0
2,0
4,0
6,0
8,0
10,0
12,0
14,0
16,0
18,0
20,0
0 20 40 60 80 100
T ime (h)
Perm
itti
vity
(pF/
cm)
0
10
20
30
40
50
60
70
Opt
ical
den
sity
OD
(600
nm)
Linear Correlation Permittivity - OD
(OD
600
nm
)
Per mi t t i v i t y ( p F / cm)
Growth onglycerol
Productionon methanol
Har
vest
Tim
e
Ind
uct
ion
Tim
e
2T1T
gas bubbles, cell debris and other particles in suspension.
- Precise and linear, up to extremely high cell densities of bacteria, filamentous fungi or flocculating yeasts- Convenient for controlling a fed-batch process, continuous fermentation, or programming an induction time based on a defined biomass target- Stable and accurate in the highly conductive media occuring in fermentation
ApplicationsFilamentous fungi fermentation monitoring
Pichia pastoris growth and fed-batch control
SoftwaresX system: an intuitive software program for plotting and recording the biomass and conductivity.It is designed for quick installation and rapid probe configurationby the user.
Biomass +: a software package to customize your Biomass System as an online, real time comprehensive cell analyzer.
Biomass Spectrometer: (optional)tracks the physiology of your cells. It
describes in detail the process dynamics and can be used to control the culture, driving it through the optimal operating range. In process development, it has proven to be a valuable technology that enables process understanding (including an alert to process deviations and identification of optimal harvesting time), which in turn leads to applications in large scale manufacturing.
cell-specific spectral fingerprint is a powerful tool for culture traceability and PAT application.
Advanced Cell Analyser: (optional)
number of viable cells and cell size, and determines cell size distribution in real time. In addition, it calculates online the cells’ doubling time.
Now you can compare online and offline data with consistency!VCD reference method, at the same time
reducing the costs and eliminating the risks of manual sampling and counting.
Technical specifications
biomass (Absidia corymbifera) in a highly aerated media.optimizes:
- Time of infection- Successful baculovirus infection- Harvest time
Measurement principleproperties of living cells; this application has the advantage of
measuring only viable cell density. Unlike optical techniques, this system is insensitive to gas bubbles, microcarriers, cell debris or other particles in suspension.
- Using FOGALE’s expertise in capacitive measurements and applying a unique and patented method, the Biomass System measures the viable biomass concentration (Biovolume), independent of cell size variations.
- By scanning the entire frequency range and with precise measurements of permittivities, the β-dispersion spectra
frequency (fc), the maximal permittivity difference (Δεmax), and the coefficient αthe morphological and physiological state of cells.
- FOGALE’s dielectric spectroscopy experience with animal cell lines brings the technique to a new level of achievement in measurement. Using a powerful patented method, the optional Advanced Cell Analyzer software
Δεmax and the cell size distribution is correlated to the α parameters.
SensorsIdeal for scale-up in a cGMP environment
Probes are made of materials that conform to FDA standards(316L stainless steel, PEEK, platinum, EPDM).12 and 25 mm diameter sensors are available to fit all port sizes,in a choice of 120, 220 and 320 mm in length to fit all vessels.All sensors are sterilizable in place (SIP) or in an autoclave.
Under the influence of an electric field, cells with intact plasma membranes (viable cells) are polarized and behave as tiny capacitors
Industrial IP 65 enclosurefor industrial environments
and can be used to automatically trigger the start of feeding or to control a proportional feed, ensuring both proper timing and dosage.
accurate online viable cell count during industrial scale production
Biomass System
Advanced Cell Analyzer
Biovolume and viable cell count
0.0
2.0
4.0
6.0
8.0
10.0
12.0
14.0
0 2 4 6 8 10 12 14
Time (days)
Per
mit
tivi
ty (
pF
/cm
)V
iab
le c
ell c
ou
nt
(E6
cell/
ml)
0.000
0.005
0.010
0.015
0.020
0.025
0.030
Bio
volu
me
FOGALE online viable cell count
Viable cell count (microscopy - trypan blue)
FOGALE Permittivity (Biovolume)
Offline Biovolume (microscopy)
Filamentous fungi fermentation monitoringAbsidia (pellets)
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
0 20 40 60 80 100 120 140 160time (h)
Pe
rmit
tiv
ity
(pF
/ cm
)
0.0
2.0
4.0
6.0
8.0
10.0
permittivityBiovolume
Linear Correlation Permittivity - Biovolume
0.0
1.0
2.0
3.0
4.0
5.0
0 1 2 3 4 5 6 7 8 9 10 11
Biovolume (µm3/ml)
Perm
itti
vit
y (
pF
/cm
)
Infe
ctio
n t
ime
Cell suspension -dispersion
0.01 0.1 1 10 100FREQUENCY (Mhz)
PE
RM
ITT
IVIT
Y (
pF
/cm
)
max
High frequency plateau
Low frequency plateau
fc (criticalfrequency)
FOGALE
= Biovolume
1 10FOGALE frequencies
to measureviable cell density
or BiovolumeConcentration range 0 -109 cell/ml for animal cells
0 - 200 g/l dry weight for yeast and bacteria
Resolution 0.01 - 106 cell/ml for animal cell0.02 g/l dry weight for yeast
Conductivity range 0.5 - 100 mS/cm
Numeric outputs / serial port RS 232 C/Mod BUS protocol data transfer
Analog outputs 4 - 20 mA or 0-10 V for Biomass / Conductivity
Number of channel monitor up to 4 probes simultaneouslyIt is possible to start with one probe, and to add more later
Dimensions Standard for rack mounting: 450 x 270 x 150 mm (19", 3U)Stainless IP 65 enclosure: 350 x 170 x 460 mm
BiomassSystem
biomass-system2.indd 2 24/09/07 22:00:49
Driving your bioreactor toward the future
For more detailed information: [email protected] / Tel. + 33 466 620 555, or visit our website at www.fogalebiotech.com
Benefits for cell cultureFrom process development to cGMP environments, the Biomass System is the choice of many cell culture engineers to optimize their bioreactor operation strategy.
Outstanding performance:
- at lab level to understand the process and/or optimize the control strategy
- for large-scale manufacturing, including the identification of process deviation, of optimal harvest time and/or PAT application
- in animal cell production, whether in suspension or attached to microcarriers
- with all of the following cell lines: CHO, PER.C6, Vero, BHK, Hybbridoma, HEK 293, NS0, Hela, Sf9, Sp2/0
ApplicationsSf9 growth and infection process by a baculovirus (BEVS)
CHO fed-batch cultivation
Benefits for fermentation
- Cell growth on glycerol- Induction time (T1)- Fed-batch on methanol- Harvest time (T2)
0,0
2,0
4,0
6,0
8,0
10,0
12,0
14,0
16,0
18,0
20,0
0 20 40 60 80 100
T ime (h)
Perm
itti
vity
(pF/
cm)
0
10
20
30
40
50
60
70
Opt
ical
den
sity
OD
(600
nm)
Linear Correlation Permittivity - OD
(OD
600
nm
)
Per mi t t i v i t y ( p F / cm)
Growth onglycerol
Productionon methanol
Har
vest
Tim
e
Ind
uct
ion
Tim
e
2T1T
gas bubbles, cell debris and other particles in suspension.
- Precise and linear, up to extremely high cell densities of bacteria, filamentous fungi or flocculating yeasts- Convenient for controlling a fed-batch process, continuous fermentation, or programming an induction time based on a defined biomass target- Stable and accurate in the highly conductive media occuring in fermentation
ApplicationsFilamentous fungi fermentation monitoring
Pichia pastoris growth and fed-batch control
SoftwaresX system: an intuitive software program for plotting and recording the biomass and conductivity.It is designed for quick installation and rapid probe configurationby the user.
Biomass +: a software package to customize your Biomass System as an online, real time comprehensive cell analyzer.
Biomass Spectrometer: (optional)tracks the physiology of your cells. It
describes in detail the process dynamics and can be used to control the culture, driving it through the optimal operating range. In process development, it has proven to be a valuable technology that enables process understanding (including an alert to process deviations and identification of optimal harvesting time), which in turn leads to applications in large scale manufacturing.
cell-specific spectral fingerprint is a powerful tool for culture traceability and PAT application.
Advanced Cell Analyser: (optional)
number of viable cells and cell size, and determines cell size distribution in real time. In addition, it calculates online the cells’ doubling time.
Now you can compare online and offline data with consistency!VCD reference method, at the same time
reducing the costs and eliminating the risks of manual sampling and counting.
Technical specifications
biomass (Absidia corymbifera) in a highly aerated media.optimizes:
- Time of infection- Successful baculovirus infection- Harvest time
Measurement principleproperties of living cells; this application has the advantage of
measuring only viable cell density. Unlike optical techniques, this system is insensitive to gas bubbles, microcarriers, cell debris or other particles in suspension.
- Using FOGALE’s expertise in capacitive measurements and applying a unique and patented method, the Biomass System measures the viable biomass concentration (Biovolume), independent of cell size variations.
- By scanning the entire frequency range and with precise measurements of permittivities, the β-dispersion spectra
frequency (fc), the maximal permittivity difference (Δεmax), and the coefficient αthe morphological and physiological state of cells.
- FOGALE’s dielectric spectroscopy experience with animal cell lines brings the technique to a new level of achievement in measurement. Using a powerful patented method, the optional Advanced Cell Analyzer software
Δεmax and the cell size distribution is correlated to the α parameters.
SensorsIdeal for scale-up in a cGMP environment
Probes are made of materials that conform to FDA standards(316L stainless steel, PEEK, platinum, EPDM).12 and 25 mm diameter sensors are available to fit all port sizes,in a choice of 120, 220 and 320 mm in length to fit all vessels.All sensors are sterilizable in place (SIP) or in an autoclave.
Under the influence of an electric field, cells with intact plasma membranes (viable cells) are polarized and behave as tiny capacitors
Industrial IP 65 enclosurefor industrial environments
and can be used to automatically trigger the start of feeding or to control a proportional feed, ensuring both proper timing and dosage.
accurate online viable cell count during industrial scale production
Biomass System
Advanced Cell Analyzer
Biovolume and viable cell count
0.0
2.0
4.0
6.0
8.0
10.0
12.0
14.0
0 2 4 6 8 10 12 14
Time (days)
Per
mit
tivi
ty (
pF
/cm
)V
iab
le c
ell c
ou
nt
(E6
cell/
ml)
0.000
0.005
0.010
0.015
0.020
0.025
0.030
Bio
volu
me
FOGALE online viable cell count
Viable cell count (microscopy - trypan blue)
FOGALE Permittivity (Biovolume)
Offline Biovolume (microscopy)
Filamentous fungi fermentation monitoringAbsidia (pellets)
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
0 20 40 60 80 100 120 140 160time (h)
Pe
rmit
tiv
ity
(pF
/ cm
)
0.0
2.0
4.0
6.0
8.0
10.0
permittivityBiovolume
Linear Correlation Permittivity - Biovolume
0.0
1.0
2.0
3.0
4.0
5.0
0 1 2 3 4 5 6 7 8 9 10 11
Biovolume (µm3/ml)
Perm
itti
vit
y (
pF
/cm
)
Infe
ctio
n t
ime
Cell suspension -dispersion
0.01 0.1 1 10 100FREQUENCY (Mhz)
PE
RM
ITT
IVIT
Y (
pF
/cm
)
max
High frequency plateau
Low frequency plateau
fc (criticalfrequency)
FOGALE
= Biovolume
1 10FOGALE frequencies
to measureviable cell density
or BiovolumeConcentration range 0 -109 cell/ml for animal cells
0 - 200 g/l dry weight for yeast and bacteria
Resolution 0.01 - 106 cell/ml for animal cell0.02 g/l dry weight for yeast
Conductivity range 0.5 - 100 mS/cm
Numeric outputs / serial port RS 232 C/Mod BUS protocol data transfer
Analog outputs 4 - 20 mA or 0-10 V for Biomass / Conductivity
Number of channel monitor up to 4 probes simultaneouslyIt is possible to start with one probe, and to add more later
Dimensions Standard for rack mounting: 450 x 270 x 150 mm (19", 3U)Stainless IP 65 enclosure: 350 x 170 x 460 mm
BiomassSystem
biomass-system2.indd 2 24/09/07 22:00:49
Driving your bioreactor toward the future
For more detailed information: [email protected] / Tel. + 33 466 620 555, or visit our website at www.fogalebiotech.com
Benefits for cell cultureFrom process development to cGMP environments, the Biomass System is the choice of many cell culture engineers to optimize their bioreactor operation strategy.
Outstanding performance:
- at lab level to understand the process and/or optimize the control strategy
- for large-scale manufacturing, including the identification of process deviation, of optimal harvest time and/or PAT application
- in animal cell production, whether in suspension or attached to microcarriers
- with all of the following cell lines: CHO, PER.C6, Vero, BHK, Hybbridoma, HEK 293, NS0, Hela, Sf9, Sp2/0
ApplicationsSf9 growth and infection process by a baculovirus (BEVS)
CHO fed-batch cultivation
Benefits for fermentation
- Cell growth on glycerol- Induction time (T1)- Fed-batch on methanol- Harvest time (T2)
0,0
2,0
4,0
6,0
8,0
10,0
12,0
14,0
16,0
18,0
20,0
0 20 40 60 80 100
T ime (h)
Perm
itti
vity
(pF/
cm)
0
10
20
30
40
50
60
70
Opt
ical
den
sity
OD
(600
nm)
Linear Correlation Permittivity - OD
(OD
600
nm
)
Per mi t t i v i t y ( p F / cm)
Growth onglycerol
Productionon methanol
Har
vest
Tim
e
Ind
uct
ion
Tim
e
2T1T
gas bubbles, cell debris and other particles in suspension.
- Precise and linear, up to extremely high cell densities of bacteria, filamentous fungi or flocculating yeasts- Convenient for controlling a fed-batch process, continuous fermentation, or programming an induction time based on a defined biomass target- Stable and accurate in the highly conductive media occuring in fermentation
ApplicationsFilamentous fungi fermentation monitoring
Pichia pastoris growth and fed-batch control
SoftwaresX system: an intuitive software program for plotting and recording the biomass and conductivity.It is designed for quick installation and rapid probe configurationby the user.
Biomass +: a software package to customize your Biomass System as an online, real time comprehensive cell analyzer.
Biomass Spectrometer: (optional)tracks the physiology of your cells. It
describes in detail the process dynamics and can be used to control the culture, driving it through the optimal operating range. In process development, it has proven to be a valuable technology that enables process understanding(including an alert to process deviations and identification of optimal harvesting time), which in turn leads to applications in large scale manufacturing.
cell-specific spectral fingerprint is a powerful tool for culture traceability and PAT application.
Advanced Cell Analyser: (optional)
number of viable cells and cell size, and determines cell size distribution in real time. In addition, it calculates online the cells’ doubling time.
Now you can compare online and offline data with consistency!VCD reference method, at the same time
reducing the costs and eliminating the risks of manual sampling and counting.
Technical specifications
biomass (Absidia corymbifera) in a highly aerated media.optimizes:
- Time of infection- Successful baculovirus infection- Harvest time
Measurement principleproperties of living cells; this application has the advantage of
measuring only viable cell density. Unlike optical techniques, this system is insensitive to gas bubbles, microcarriers, cell debris or other particles in suspension.
- Using FOGALE’s expertise in capacitive measurements and applying a unique and patented method, the Biomass System measures the viable biomass concentration (Biovolume), independent of cell size variations.
- By scanning the entire frequency range and with precise measurements of permittivities, the β-dispersion spectra
frequency (fc), the maximal permittivity difference (Δεmax), and the coefficient αthe morphological and physiological state of cells.
- FOGALE’s dielectric spectroscopy experience with animal cell lines brings the technique to a new level of achievement in measurement. Using a powerful patented method, the optional Advanced Cell Analyzer software
Δεmax and the cell size distribution is correlated to the α parameters.
SensorsIdeal for scale-up in a cGMP environment
Probes are made of materials that conform to FDA standards(316L stainless steel, PEEK, platinum, EPDM).12 and 25 mm diameter sensors are available to fit all port sizes,in a choice of 120, 220 and 320 mm in length to fit all vessels.All sensors are sterilizable in place (SIP) or in an autoclave.
Under the influence of an electric field, cells with intact plasma membranes (viable cells) are polarized and behave as tiny capacitors
Industrial IP 65 enclosurefor industrial environments
and can be used to automatically trigger the start of feeding or to control a proportional feed, ensuring both proper timing and dosage.
accurate online viable cell count during industrial scale production
Biomass System
Advanced Cell Analyzer
Biovolume and viable cell count
0.0
2.0
4.0
6.0
8.0
10.0
12.0
14.0
0 2 4 6 8 10 12 14
Time (days)
Per
mit
tivi
ty (
pF
/cm
)V
iab
le c
ell c
ou
nt
(E6
cell/
ml)
0.000
0.005
0.010
0.015
0.020
0.025
0.030
Bio
volu
me
FOGALE online viable cell count
Viable cell count (microscopy - trypan blue)
FOGALE Permittivity (Biovolume)
Offline Biovolume (microscopy)
Filamentous fungi fermentation monitoringAbsidia (pellets)
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
0 20 40 60 80 100 120 140 160time (h)
Pe
rmit
tiv
ity
(pF
/ cm
)
0.0
2.0
4.0
6.0
8.0
10.0
permittivityBiovolume
Linear Correlation Permittivity - Biovolume
0.0
1.0
2.0
3.0
4.0
5.0
0 1 2 3 4 5 6 7 8 9 10 11
Biovolume (µm3/ml)
Perm
itti
vit
y (
pF
/cm
)
Infe
ctio
n t
ime
Cell suspension -dispersion
0.01 0.1 1 10 100FREQUENCY (Mhz)
PE
RM
ITT
IVIT
Y (
pF
/cm
)
max
High frequency plateau
Low frequency plateau
fc (criticalfrequency)
FOGALE
= Biovolume
1 10FOGALE frequencies
to measureviable cell density
or BiovolumeConcentration range 0 -109 cell/ml for animal cells
0 - 200 g/l dry weight for yeast and bacteria
Resolution 0.01 - 106 cell/ml for animal cell0.02 g/l dry weight for yeast
Conductivity range 0.5 - 100 mS/cm
Numeric outputs / serial port RS 232 C/Mod BUS protocol data transfer
Analog outputs 4 - 20 mA or 0-10 V for Biomass / Conductivity
Number of channel monitor up to 4 probes simultaneouslyIt is possible to start with one probe, and to add more later
Dimensions Standard for rack mounting: 450 x 270 x 150 mm (19", 3U)Stainless IP 65 enclosure: 350 x 170 x 460 mm
BiomassSystem
biomass-system2.indd 2 24/09/07 22:26:45
Driving your bioreactor toward the future
For more detailed information: [email protected] / Tel. + 33 466 620 555, or visit our website at www.fogalebiotech.com
Benefits for cell cultureFrom process development to cGMP environments, the Biomass System is the choice of many cell culture engineers to optimize their bioreactor operation strategy.
Outstanding performance:
- at lab level to understand the process and/or optimize the control strategy
- for large-scale manufacturing, including the identification of process deviation, of optimal harvest time and/or PAT application
- in animal cell production, whether in suspension or attached to microcarriers
- with all of the following cell lines: CHO, PER.C6, Vero, BHK, Hybbridoma, HEK 293, NS0, Hela, Sf9, Sp2/0
ApplicationsSf9 growth and infection process by a baculovirus (BEVS)
CHO fed-batch cultivation
Benefits for fermentation
- Cell growth on glycerol- Induction time (T1)- Fed-batch on methanol- Harvest time (T2)
0,0
2,0
4,0
6,0
8,0
10,0
12,0
14,0
16,0
18,0
20,0
0 20 40 60 80 100
T ime (h)
Perm
itti
vity
(pF/
cm)
0
10
20
30
40
50
60
70
Opt
ical
den
sity
OD
(600
nm)
Linear Correlation Permittivity - OD
(OD
600
nm
)
Per mi t t i v i t y ( p F / cm)
Growth onglycerol
Productionon methanol
Har
vest
Tim
e
Ind
uct
ion
Tim
e
2T1T
gas bubbles, cell debris and other particles in suspension.
- Precise and linear, up to extremely high cell densities of bacteria, filamentous fungi or flocculating yeasts- Convenient for controlling a fed-batch process, continuous fermentation, or programming an induction time based on a defined biomass target- Stable and accurate in the highly conductive media occuring in fermentation
ApplicationsFilamentous fungi fermentation monitoring
Pichia pastoris growth and fed-batch control
SoftwaresX system: an intuitive software program for plotting and recording the biomass and conductivity.It is designed for quick installation and rapid probe configurationby the user.
Biomass +: a software package to customize your Biomass System as an online, real time comprehensive cell analyzer.
Biomass Spectrometer: (optional)tracks the physiology of your cells. It
describes in detail the process dynamics and can be used to control the culture, driving it through the optimal operating range. In process development, it has proven to be a valuable technology that enables process understanding (including an alert to process deviations and identification of optimal harvesting time), which in turn leads to applications in large scale manufacturing.
cell-specific spectral fingerprint is a powerful tool for culture traceability and PAT application.
Advanced Cell Analyser: (optional)
number of viable cells and cell size, and determines cell size distribution in real time. In addition, it calculates online the cells’ doubling time.
Now you can compare online and offline data with consistency!VCD reference method, at the same time
reducing the costs and eliminating the risks of manual sampling and counting.
Technical specifications
biomass (Absidia corymbifera) in a highly aerated media.optimizes:
- Time of infection- Successful baculovirus infection- Harvest time
Measurement principleproperties of living cells; this application has the advantage of
measuring only viable cell density. Unlike optical techniques, this system is insensitive to gas bubbles, microcarriers, cell debris or other particles in suspension.
- Using FOGALE’s expertise in capacitive measurements and applying a unique and patented method, the Biomass System measures the viable biomass concentration (Biovolume), independent of cell size variations.
- By scanning the entire frequency range and with precise measurements of permittivities, the β-dispersion spectra
frequency (fc), the maximal permittivity difference (Δεmax), and the coefficient αthe morphological and physiological state of cells.
- FOGALE’s dielectric spectroscopy experience with animal cell lines brings the technique to a new level of achievement in measurement. Using a powerful patented method, the optional Advanced Cell Analyzer software
Δεmax and the cell size distribution is correlated to the α parameters.
SensorsIdeal for scale-up in a cGMP environment
Probes are made of materials that conform to FDA standards(316L stainless steel, PEEK, platinum, EPDM).12 and 25 mm diameter sensors are available to fit all port sizes,in a choice of 120, 220 and 320 mm in length to fit all vessels.All sensors are sterilizable in place (SIP) or in an autoclave.
Under the influence of an electric field, cells with intact plasma membranes (viable cells) are polarized and behave as tiny capacitors
Industrial IP 65 enclosurefor industrial environments
and can be used to automatically trigger the start of feeding or to control a proportional feed, ensuring both proper timing and dosage.
accurate online viable cell count during industrial scale production
Biomass System
Advanced Cell Analyzer
Biovolume and viable cell count
0.0
2.0
4.0
6.0
8.0
10.0
12.0
14.0
0 2 4 6 8 10 12 14
Time (days)
Per
mit
tivi
ty (
pF
/cm
)V
iab
le c
ell c
ou
nt
(E6
cell/
ml)
0.000
0.005
0.010
0.015
0.020
0.025
0.030
Bio
volu
me
FOGALE online viable cell count
Viable cell count (microscopy - trypan blue)
FOGALE Permittivity (Biovolume)
Offline Biovolume (microscopy)
Filamentous fungi fermentation monitoringAbsidia (pellets)
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
0 20 40 60 80 100 120 140 160time (h)
Pe
rmit
tiv
ity
(pF
/ cm
)
0.0
2.0
4.0
6.0
8.0
10.0
permittivityBiovolume
Linear Correlation Permittivity - Biovolume
0.0
1.0
2.0
3.0
4.0
5.0
0 1 2 3 4 5 6 7 8 9 10 11
Biovolume (µm3/ml)
Perm
itti
vit
y (
pF
/cm
)
Infe
ctio
n t
ime
Cell suspension -dispersion
0.01 0.1 1 10 100FREQUENCY (Mhz)
PE
RM
ITT
IVIT
Y (
pF
/cm
)
max
High frequency plateau
Low frequency plateau
fc (criticalfrequency)
FOGALE
= Biovolume
1 10FOGALE frequencies
to measureviable cell density
or BiovolumeConcentration range 0 -109 cell/ml for animal cells
0 - 200 g/l dry weight for yeast and bacteria
Resolution 0.01 - 106 cell/ml for animal cell0.02 g/l dry weight for yeast
Conductivity range 0.5 - 100 mS/cm
Numeric outputs / serial port RS 232 C/Mod BUS protocol data transfer
Analog outputs 4 - 20 mA or 0-10 V for Biomass / Conductivity
Number of channel monitor up to 4 probes simultaneouslyIt is possible to start with one probe, and to add more later
Dimensions Standard for rack mounting: 450 x 270 x 150 mm (19", 3U)Stainless IP 65 enclosure: 350 x 170 x 460 mm
BiomassSystem
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CELL CULTURE & FERMENTATION
Driving your bioreactor toward the future
FOGALE nanotech
Access incredible benefitswith the FOGALE Biomass System
viable cell density for yeast, bacterial, fungal, animal and plant cells.
It is suitable for the measurement of both very low and very high cell concentrations in complex media.
With additional software programs, it can be transformed into a full Biomass Analyzers.
For more detailed information: [email protected] / Tel. + 33 466 620 555, or visit our website at www.fogalebiotech.com
Online Biomass monitoring
Measures in real time and records all growthMonitors cell density in the culture mediaGreatly decreases contamination risks during samplingReduces the need for intensive lab analysis & eliminates human error
Biomass control has never been so easy !
An ideal instrument for closed loop control in batch, fed-batch and perfusionKeeping your cell density constant or optimizing your feed rate and timing is now very convenient
FOGALE Nanotech, knownas the worldwide reference for
viable biomass monitoringbased on capacitance, provides
specific standard & integrated solutions.
Wherever your business is located, our worldwide sup-port provides the service you deserve.Our team of experts will help you set up new systemsand will provide full-scope customer service.
Our sensors and systems, our experience and know-how, our professionalism and commitment are the gua-rantee of your entire satisfaction.
For more information about our products,please call us at +33 (0) 466 620 555or visit our website www.fogalebiotech.com
and all the advantages you wantavailable in a single model...
Stable and sensitivein all culture conditions
Not limitedby media conductivity
Modular designfrom 1 to 4 probes on the same control unit
Compact designprobes and pre-amplifiers fit all vessel sizes
User friendlyquick installation and easy handling
Frequency scanning and advanced softwaressystem upgradable at any time
...ALL for the best priceDistributor
Driving your bioreactor toward the future
Expertise and Performance
Optimize yield and shorten process development Increase control of your culture during scale-up Improve the level and quality of and traceabilityin production
Europe/Asia: FOGALE nanotech, France +33 466 620 555 - [email protected]
USA: FOGALE nanotech Inc, Los Angeles,CA / (310) 348 2974 - [email protected]
biomass-system2.indd 1 24/09/07 22:00:15