form disertasi doktor - universitas brawijaya · pada media pikovskaya. isolat yang mempunyai...
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LAPORAN HASIL PENELITIAN DISERTASI DOKTOR TAHUN ANGGARAN 2011
ISOLASI, KARAKTERISASI & UJI AKTIFITAS MIKROBA PELARUT FOSFAT DAN PENGIKAT NITROGEN DARI MOL (Mikroorganisme
Lokal) BONGGOL & BATANG PISANG (Musa paradisiaca)
SUSLAM PRATAMANINGTYAS
Dibiayai Oleh Direktorat Jenderal Pendidikan Tinggi, Kementerian Pendidikan Nasional, melalui DIPA Universitas Brawijaya REV.1 Nomor: 0636/023-04.2.16/15/2011 R,
tanggal 30 Maret 2011 dan berdasarkan Surat Keputusan Rektor Nomor: 214/SK/2011 tanggal 2 Mei 2011
UNIVERSITAS BRAWIJAYA
NOVEMBER 2011
RINGKASAN DAN SUMMARY
Budidaya suatu jenis tanaman secara terus menerus secara intensif membawa
konsekuensi pengurasan hara tanah yang berakibat menurunnya produktifitas lahan
yang bersangkutan. Salah satu indikator menurunnya produktifitas lahan ini adalah
tidak efektifnya pemupukan. Penambahan dosis pupuk yang dimaksudkan untuk
mendongkrak produksi tidak dapat memberikan hasil seperti yang diharapkan.
Dilatarbelakangi oleh kondisi tersebut, sekelompok petani padi di Kecamatan Sukorejo,
Kabupaten Pasuruan Jawa Timur, berinisiatif membuat pupuk cair dari bahan-bahan
sisa panen yang tersedia di lingkungan setempat, diantaranya adalah pupuk MOL
bonggol pisang dan MOL batang pisang, yang ‘dianggap’ dapat menggantikan pupuk
sintetis Urea dan SP36.
Hasil analisis laboratorium menunjukkan bahwa kandungan nitrogen dan fosfor
dari pupuk MOL terlalu rendah untuk bisa disebut sebagai pengganti pupuk sintetis.
Isolasi, identifikasi dan uji kemampuan mengikat nitrogen dan kemampuan melarutkan
fosfat oleh mikroorganisme yang dikandung MOL bonggol dan batang pisang dilakukan
di Laboratorium Biologi, Fakultas Pertanian Universitas Widyagama Malang.
Beberapa isolat fungi dan bakteri telah berhasil diisolasi. Dari semua isolat fungi
yang ditemukan, tidak teridentifikasi ada isolat yang mampu mengikat nirogen bebas
maupun yang mempunyai kemampuan melarutkan fosfat. Semua isolat bakteri juga
tidak teridentifikasi mempunyai kemampuan mengikat nitrogen bebas, namun
ditemukan satu isolat yang mempunyai kemampuan kuat dan cepat dalam melarutkan
fosfat.
Pengujian kemampuan mikroba mengikat nitrogen bebas dilakukan dengan
inokulasi pada media Burk’s N-free. Apabila isolat mampu tumbuh, berarti isolat yang
bersangkutan mempunyai kemampuan mengikat nitrogen bebas untuk
pertumbuhannya.
Pengujian kemampuan mikroba melarutkan fosfat dilakukan dengan inokulasi
pada media Pikovskaya. Isolat yang mempunyai kemampuan melarutkan fosfat ditandai
oleh terbentuknya halozone disekitar koloni
Pengujian biokmia untuk identifikasi adalah Uji Gram & KOH 3%, Uji Katalase,
dan beberapa uji lain sesuai standar pengujian idetifikasi bakteri menurut Bergey’s
Manual of Determinative Bacteriology. Hasil Uji untuk identifikasi menyimpulkan bahwa
isolat – 1 adalah Serratia marcescens.
Hasil Uji pathogenisitas menyimpulkan bahwa strain Serratia marcescens yang
ditemukan tidak besifat pathogen untuk padi (Oryza sativa). Kemampuan isolat-1
melarutkan P secara kualitatif adalah rata-rata Ø halozone adalah 1,95 Cm, sedangkan
kemampuan melarutkan P secara kuantitatif adalah 357 mg/L. Produksi asam yang
dihasilkan isolat-1 Serratia marcescens mampu menurunkan pH media menjadi 5,05
dibandingkan pH pada media kontrol 6,25.
SUMMARY
Intensive cultivation of one plant species continuously, have a consequences to
soil nutrient depletion and resulting in declining productivity of land were concerned. the
One of declining indicator of land productivity is uneffective fertilization. The addition of
fertilizer dose that is intended to boost production can not deliver results as expected.
Motivated by these conditions, a group of rice farmers in the Sukorejo sub-district,
Pasuruan regency-East Java, took the initiative to make liquid fertilizer from the
harvesting residual which available in the local environment, including the indigenous
microorganism (IMO) fertilizer banana root and pseudo-stem, which are 'deemed' can
replace the synthetic fertilizers Urea and SP-36
The results of laboratory analysis indicates that the content of nitrogen and
phosphorus from IMO fertilizers are too low to be called as a substitute for synthetic
fertilizers. the isolation, identification and the test of the nitrogen fixing ability and the
phosphate solubilizing ability of the microorganisms were contained in IMO from banana
root and pseudo-stem were performed at the Laboratory of Biology, Agricultural Faculty-
Widyagama.Malang University
Some isolates of fungi and bacteria have been isolated. All of the fungal isolates
were found, are not identified as free nitrogen fixing or have the ability to solubilize
phosphate. All of bactery isolates were also unidentified having the ability to fixing the
free nitrogen, but one isolate was found has a strong ability and fast to solubilize
phosphate.
To testing the ability of microbial to fixing the free-nitrogen is performed by
inoculation it on Burk's N-free. When the isolates were able to grow, it means that the
isolates have the ability to fixing the free-nitrogen for it's growth.
To testing the phosphate solubilizing ability of microbes was carried out by
inoculating to the Pikovskaya medium. Isolates that have the ability to solubilize
phosphate is characterized by the formation of halozone around the colonies.
Biochemist testing for identification of bacteria is Gram Test & KOH-3% test,
Catalase Test, and several other tests according to bacteria identification testing
standards according to Bergey's Manual of Determinative Bacteriology.
Test results for the identification concluded that isolates - 1 is Serratia
marcescens. Results for pathogenisity test concluded that the strain Serratia
marcescens was found that its not tend to be pathogen for rice (Oryza sativa). The
qualitative ability of isolate-1 to solubilize P were shown with Ø halozone average is
1.95 cm, while the quantitative ability to solubilize P is 357 mg / L. The acid production
result of Serratia marcescens isolate-1 have capable to lowering the pH of the media to
5.05 compared to control medium pH at 6.25.
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