From Natural Diversityto Better Cultivars

10 January 2013San Diego, California

Evaluation of needs and priorities has been ongoing in the current RosBREED project: • Information of trait values from stakeholders

(Obj. 1 of RB1)

• Information of MAB breeding needs and opportunities from breeders (Obj. 5 of RB1)

• Input from Advisory Panel Members(Obj. 5 of RB1)

Direction from Feedback

Feedback Received

• Industry:Risks and opportunities indicate a need to broaden the scope of traits while making sure that fruit quality is still a top priority

• Breeders:MAB adoption has begun, but breeders need help (access to routine accurate genotyping services, genetic knowledge for other traits, implications of trait locus clusters, and knowledge of genotype × environment interaction)

Planning Activities in 2012

• Advisory Panel Members: recommendation to consider a second project

• U.S. Rosaceae Executive Committee: participation in conference calls and meetings

• U.S. Rosaceae breeders and interested colleagues: written input solicited, involved in planning process

• Planning group: developed Logic Model

• Planning group: identified scope, desired outcomes, and objectives

• Advisory Panel members: provide input on scope, desired outcomes, and objectivesTO

DAY

More rapid availability of new

cultivars with genetically superior

fruit quality

Improved profitability and sustainability of

U.S. rosaceous fruit, nut, and floral crops

with increased consumption and

enjoyment

RosBREED’s Long Term Outcomes

RosBREED Approach

Improve the efficiency and successof U.S. Rosaceae crop breeding programs

through the use of DNA diagnostic tests

RosBREED #2

IMPLEMENTATION

DISCOVERYPre-breeding

Post-breeding

Breeding

Plant diversity identified to

address risks & opportunities

Risks mitigated, opportunities

realized

Wild germplasm

Elite germplasm

Cultivar release & adoption

Objectives Structure

Obj. 1. Central breeding support

Obj. 2. New trait locus discovery

Obj. 3. Pre-breeding

Obj. 4. Post-breeding

Obj. 5. Communicate project outcomes & impacts

WA2

Which wild germplasm to use?

Which parents & combinations to create?

Which seedlings to progress?

Which selections to trial?

Which advanced selections to commercialize?

Supporting Breeding Decisions

Obj. 1: Central Breeding Support

Goal: Expand support tools Rosaceae-wide for efficient processing of new cultivar-generating plant populations addressing production and market risks and opportunities

Success: Valuable & superior new genetic combinations efficiently, accurately, rapidly, and creatively developed and identified; Massive complex breeding data managed efficiently for routine implementation of DNA-informed breeding

RosBREED #2

Demonstration Breeding Programs

Opportunities Addressed

• Advances in comparative genomics will allow for more efficient genetic discovery across crops

• A new statistical approach (Genome-wide selection) has potential to

increase the precision of marker-assisted breeding

• Advances by international groups/projectswill be leveraged

Challenges Addressed

• Access to accurate, cost effective genotyping services

• Database resources and tools to support MAB

• Polyploidy: definingfunctional alleles &

allele dosage

Obj. 2: New trait locus discovery

Goal: Ensure known trait loci are available for high-priority traits addressing production and market risks and opportunities

Success: All U.S. Rosaceae breeding programs have access to promising known trait loci addressing high value traits

WA2

Which wild germplasm to use?

Which parents & combinations to create?

Which seedlings to progress?

Which selections to trial?

Which advanced selections to commercialize?

Supporting Pre-Breeding Decisions

Obj. 3: Pre-breeding

Goal: Provide access to genepools of wild and novel germplasm for rosaceous crop breeders

Success: Valuable alleles from wild or novel germplasm are routinely identified and incorporated into cultivar-generating populations more efficiently, accurately, rapidlyand creatively

WA2

Which wild germplasm to use?

Which parents & combinations to create?

Which seedlings to progress?

Which selections to trial?

Which advanced selections to commercialize?

Supporting Post-Breeding Decisions

Obj. 4: Post-breeding

Goal: Enable and optimize use of DNA information for elite selection advancement and cultivar adoption decisions

Success: Breeders and industry sectors will make confident elite selection advancement and cultivar adoption decisions, thereby mitigating risks and realizing opportunities

Obj. 5: Communicate project outcomes and impacts

Goal: Communicated project outcomes and impacts to all stakeholders

Success: Breeders, allied scientists, industry sectors, and the public have an increased understanding of how genetics knowledge is being used to more efficiently and rapidly develop and commercialized new rosaceous cultivars that provide increased consumption, enjoyment, profitability, and sustainability

U.S.-Wide and Multi-Disciplinary

BREEDING

Genetics

Genomics

Bioinformatics

Computer Programming

Socio Economics

Evaluation

Communication

Technology Transfer

Plant Research International, NetherlandsEast Malling Research, UKCRA-FRU, Rome, ItalyIASMA, Trento, ItalyINRA (Bordeaux, Avignon, Angers), France

Andres Bello University, Chile Plant & Food Research, New Zealand

ARC, South Africa

RosBREED participant

location

International Collaborators

+ 2 Scientific Advisory Panel members (Spain, New Zealand)

AcknowledgementContributors to Logic Model

Cameron Peace Amy Iezzoni Robert ShuppNahla Bassil Sook Jung Eric van de WegRex Bernardo Jim Luby Gayle VolkMichael Coe Dorrie Main Vance WhitakerTom Davis Chris Maliepaard Chengyan YueKate Evans Vicki McCrackenGennaro Fazio Jim McFersonKarina Gallardo Jay NorelliKsenija Gasic Mercy OlmsteadCraig Hardner Brad Rickard

Advisory Panel Members

RosBREED I First Annual Meeting, 7-8 Jan 2010

RosBREED II Annual Advisory Panel Meeting, 13 Jan 2011

Thank you