fruit tree clean plant center wsu - iarec. new personnel: james susaimuthu supervises virus...
TRANSCRIPT
Fruit Tree Clean Plant CenterWSU - IAREC
New personnel:James Susaimuthu
• supervises virus indexing
Sam Hood• manages growth facilities
Terri Reddout• assists FTCPN management
Martin Joseph• directs tissue culture program
Infrastructure improvements:• Greenhouse renovations
• Complete• Biosecurity • Improved environmental control
• Relocate thermal therapy facility • Near complete• Biosecurity
• New greenhouse (industry funded)• Design complete• Replaces deteriorated structure• Biosecurity
Asbestos bench tops
Gravel floor
Deteriorated glazing
Replacement quarantine greenhouse• designed
• major components purchased
Upgraded irrigation system
Budwood distributions:• Domestic releases 27,277• International releases 1,618
• Representing 13 countries
Selections received:• Proprietary 55• Foreign 28• Public 17
• Indexing only 72
• Heat therapy 20
Thermal therapy•130 selections going through heat therapy• Includes those received in previous years
Current testing cycle in progress:•Woody indexing tests 20,000•Herbaceous indexing (Mar) 600•Molecular assays 1,500•ELISAs 1,500
Malus: Required Actual
Woody indexing 5 X 4 reps each 5 X 4 reps each
Herbaceous indexing
4 4
Field indexing 4 4
PCR / RT-PCR 2 5 + 4 to confirm
ELISA - -
Stone fruit:Woody indexing 8 X 4 reps each 8 X 4 reps each
Herbaceous indexing
4 4
Field indexing 1 1
PCR / RT-PCR 3 7 + 3 to confirm
ELISA 4 4
Current FTCPC research activities:
CVA – capillovirus• ~ 50% of cherry selections infected• Likely difficult to remove via thermal therapy• Impact unknown
Apple green crinkle disease• 3 cropping years is required protocol• Identify pathogen associated with disease
Rusty mottle / Twisted leaf• Genetically complex family of viruses• May be able to eliminate some woody indexing