full wwpdb em validation report o i · 2020. 12. 6. · page 2 ullf wwpdb em alidationv report...
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Full wwPDB EM Validation Report iO
Dec 6, 2020 � 04:47 am GMT
PDB ID : 6FYXEMDB ID : EMD-4327
Title : Structure of a partial yeast 48S preinitiation complex with eIF5 N-terminaldomain (model C1)
Authors : Llacer, J.L.; Hussain, T.; Gordiyenko, Y.; Ramakrishnan, V.Deposited on : 2018-03-12Resolution : 3.05 Å(reported)
This is a Full wwPDB EM Validation Report for a publicly released PDB entry.
We welcome your comments at [email protected] user guide is available at
https://www.wwpdb.org/validation/2017/EMValidationReportHelpwith speci�c help available everywhere you see the iO symbol.
The following versions of software and data (see references iO) were used in the production of this report:
EMDB validation analysis : 0.0.0.dev61Mogul : 1.8.5 (274361), CSD as541be (2020)
MolProbity : 4.02b-467buster-report : 1.1.7 (2018)
Percentile statistics : 20191225.v01 (using entries in the PDB archive December 25th 2019)Ideal geometry (proteins) : Engh & Huber (2001)
Ideal geometry (DNA, RNA) : Parkinson et al. (1996)Validation Pipeline (wwPDB-VP) : 2.15.1
https://www.wwpdb.org/validation/2017/EMValidationReportHelphttps://www.wwpdb.org/validation/2017/EMValidationReportHelphttps://www.wwpdb.org/validation/2017/EMValidationReportHelphttps://www.wwpdb.org/validation/2017/EMValidationReportHelp#references
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Page 2 Full wwPDB EM Validation Report EMD-4327, 6FYX
1 Overall quality at a glance iO
The following experimental techniques were used to determine the structure:ELECTRON MICROSCOPY
The reported resolution of this entry is 3.05 Å.
Percentile scores (ranging between 0-100) for global validation metrics of the entry are shown inthe following graphic. The table shows the number of entries on which the scores are based.
MetricWhole archive(#Entries)
EM structures(#Entries)
Ramachandran outliers 154571 4023Sidechain outliers 154315 3826RNA backbone 4643 859
The table below summarises the geometric issues observed across the polymeric chains and their �tto the map. The red, orange, yellow and green segments of the bar indicate the fraction of residuesthat contain outliers for >=3, 2, 1 and 0 types of geometric quality criteria respectively. A greysegment represents the fraction of residues that are not modelled. The numeric value for eachfraction is indicated below the corresponding segment, with a dot representing fractions
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Page 3 Full wwPDB EM Validation Report EMD-4327, 6FYX
Continued from previous page...
Mol Chain Length Quality of chain
9 F 227
10 G 236
11 H 190
12 I 201
13 J 188
14 K 106
15 L 156
16 M 134
17 N 151
18 O 137
19 P 142
20 Q 143
21 R 136
22 S 146
23 T 144
24 U 117
25 V 87
26 W 130
27 X 145
28 Y 135
29 Z 108
30 a 119
31 b 82
32 c 67
33 d 56Continued on next page...
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Page 4 Full wwPDB EM Validation Report EMD-4327, 6FYX
Continued from previous page...
Mol Chain Length Quality of chain
34 e 63
35 f 150
36 g 326
37 h 25
38 i 153
39 j 304
40 k 527
41 l 285
42 m 405
43 o 964
44 p 763
45 q 812
46 r 274
47 s 347
The following table lists non-polymeric compounds, carbohydrate monomers and non-standardresidues in protein, DNA, RNA chains that are outliers for geometric or electron-density-�t crite-ria:
Mol Type Chain Res Chirality Geometry Clashes Electron density2 C4J 2 1190 X - - -
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Page 5 Full wwPDB EM Validation Report EMD-4327, 6FYX
2 Entry composition iO
There are 51 unique types of molecules in this entry. The entry contains 104404 atoms, of which0 are hydrogens and 0 are deuteriums.
In the tables below, the AltConf column contains the number of residues with at least one atomin alternate conformation and the Trace column contains the number of residues modelled with atmost 2 atoms.
Molecule 1 is a RNA chain called tRNAi.
Mol Chain Residues Atoms AltConf Trace
1 1 75Total C N O P1639 734 298 531 76
0 0
Molecule 2 is a RNA chain called 18S ribosomal RNA.
Mol Chain Residues Atoms AltConf Trace
2 2 1780Total C N O P37812 16904 6659 12469 1780
0 0
There are 2 discrepancies between the modelled and reference sequences:
Chain Residue Modelled Actual Comment Reference2 676 G U con�ict GB 496422082 678 U G con�ict GB 49642208
Molecule 3 is a RNA chain called mRNA (31-MER).
Mol Chain Residues Atoms AltConf Trace
3 3 31Total C N O P719 324 108 252 35
4 0
Molecule 4 is a protein called 40S ribosomal protein S0.
Mol Chain Residues Atoms AltConf Trace
4 A 219Total C N O S1702 1085 299 316 2
0 0
Molecule 5 is a protein called 40S ribosomal protein S1.
Mol Chain Residues Atoms AltConf Trace
5 B 225Total C N O S1797 1135 330 329 3
0 0
https://www.wwpdb.org/validation/2017/EMValidationReportHelp#entry_composition
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Page 6 Full wwPDB EM Validation Report EMD-4327, 6FYX
Molecule 6 is a protein called KLLA0F09812p.
Mol Chain Residues Atoms AltConf Trace
6 C 220Total C N O S1648 1053 291 300 4
0 0
Molecule 7 is a protein called KLLA0D08305p.
Mol Chain Residues Atoms AltConf Trace
7 D 227Total C N O S1774 1126 320 323 5
0 0
Molecule 8 is a protein called 40S ribosomal protein S4.
Mol Chain Residues Atoms AltConf Trace
8 E 260Total C N O S2078 1322 393 359 4
0 0
Molecule 9 is a protein called KLLA0D10659p.
Mol Chain Residues Atoms AltConf Trace
9 F 206Total C N O S1609 1008 298 300 3
0 0
Molecule 10 is a protein called 40S ribosomal protein S6.
Mol Chain Residues Atoms AltConf Trace
10 G 230Total C N O S1832 1146 352 330 4
0 0
Molecule 11 is a protein called 40S ribosomal protein S7.
Mol Chain Residues Atoms AltConf Trace
11 H 184Total C N O1483 950 270 263
0 0
Molecule 12 is a protein called 40S ribosomal protein S8.
Mol Chain Residues Atoms AltConf Trace
12 I 188Total C N O S1489 923 300 265 1
0 0
Molecule 13 is a protein called KLLA0E23673p.
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Page 7 Full wwPDB EM Validation Report EMD-4327, 6FYX
Mol Chain Residues Atoms AltConf Trace
13 J 182Total C N O S1471 929 287 254 1
0 0
Molecule 14 is a protein called KLLA0B08173p.
Mol Chain Residues Atoms AltConf Trace
14 K 96Total C N O S809 533 129 146 1
0 0
Molecule 15 is a protein called KLLA0A10483p.
Mol Chain Residues Atoms AltConf Trace
15 L 155Total C N O S1248 798 237 210 3
0 0
Molecule 16 is a protein called 40S ribosomal protein S12.
Mol Chain Residues Atoms AltConf Trace
16 M 117Total C N O885 553 161 171
0 0
Molecule 17 is a protein called KLLA0F18040p.
Mol Chain Residues Atoms AltConf Trace
17 N 151Total C N O S1195 761 224 207 3
0 0
Molecule 18 is a protein called 40S ribosomal protein S14.
Mol Chain Residues Atoms AltConf Trace
18 O 129Total C N O S954 584 191 176 3
0 0
Molecule 19 is a protein called KLLA0F07843p.
Mol Chain Residues Atoms AltConf Trace
19 P 117Total C N O S923 592 165 161 5
0 0
Molecule 20 is a protein called 40S ribosomal protein S16.
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Page 8 Full wwPDB EM Validation Report EMD-4327, 6FYX
Mol Chain Residues Atoms AltConf Trace
20 Q 141Total C N O1105 709 204 192
0 0
Molecule 21 is a protein called KLLA0B01474p.
Mol Chain Residues Atoms AltConf Trace
21 R 130Total C N O S1033 643 194 193 3
0 0
Molecule 22 is a protein called KLLA0B01562p.
Mol Chain Residues Atoms AltConf Trace
22 S 145Total C N O S1189 739 239 209 2
0 0
Molecule 23 is a protein called KLLA0A07194p.
Mol Chain Residues Atoms AltConf Trace
23 T 143Total C N O1110 693 210 207
0 0
Molecule 24 is a protein called KLLA0F25542p.
Mol Chain Residues Atoms AltConf Trace
24 U 106Total C N O S845 540 152 152 1
0 0
Molecule 25 is a protein called 40S ribosomal protein S21.
Mol Chain Residues Atoms AltConf Trace
25 V 87Total C N O S687 424 126 135 2
0 0
Molecule 26 is a protein called 40S ribosomal protein S22.
Mol Chain Residues Atoms AltConf Trace
26 W 129Total C N O S1021 651 187 180 3
0 0
Molecule 27 is a protein called KLLA0B11231p.
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Page 9 Full wwPDB EM Validation Report EMD-4327, 6FYX
Mol Chain Residues Atoms AltConf Trace
27 X 144Total C N O S1119 708 218 191 2
0 0
Molecule 28 is a protein called 40S ribosomal protein S24.
Mol Chain Residues Atoms AltConf Trace
28 Y 134Total C N O1061 665 207 189
0 0
Molecule 29 is a protein called KLLA0B06182p.
Mol Chain Residues Atoms AltConf Trace
29 Z 78Total C N O S594 376 111 106 1
0 0
Molecule 30 is a protein called 40S ribosomal protein S26.
Mol Chain Residues Atoms AltConf Trace
30 a 103Total C N O S812 500 173 134 5
0 0
Molecule 31 is a protein called 40S ribosomal protein S27.
Mol Chain Residues Atoms AltConf Trace
31 b 81Total C N O S609 379 112 113 5
0 0
Molecule 32 is a protein called 40S ribosomal protein S28.
Mol Chain Residues Atoms AltConf Trace
32 c 64Total C N O S499 308 99 91 1
0 0
Molecule 33 is a protein called 40S ribosomal protein S29.
Mol Chain Residues Atoms AltConf Trace
33 d 55Total C N O S461 289 93 78 1
0 0
Molecule 34 is a protein called 40S ribosomal protein S30.
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Page 10 Full wwPDB EM Validation Report EMD-4327, 6FYX
Mol Chain Residues Atoms AltConf Trace
34 e 60Total C N O S472 295 96 80 1
0 0
Molecule 35 is a protein called Ubiquitin-40S ribosomal protein S27a.
Mol Chain Residues Atoms AltConf Trace
35 f 74Total C N O S584 374 111 95 4
0 0
Molecule 36 is a protein called KLLA0E12277p.
Mol Chain Residues Atoms AltConf Trace
36 g 320Total C N O S2469 1561 432 471 5
0 0
Molecule 37 is a protein called 60S ribosomal protein L41-A.
Mol Chain Residues Atoms AltConf Trace
37 h 25Total C N O S233 142 63 27 1
0 0
Molecule 38 is a protein called Eukaryotic translation initiation factor 1A.
Mol Chain Residues Atoms AltConf Trace
38 i 121Total C N O S958 587 183 183 5
0 0
Molecule 39 is a protein called Eukaryotic translation initiation factor 2 subunit alpha.
Mol Chain Residues Atoms AltConf Trace
39 j 249Total C N O S2006 1283 333 382 8
0 0
Molecule 40 is a protein called Eukaryotic translation initiation factor 2 subunit gamma.
Mol Chain Residues Atoms AltConf Trace
40 k 414Total C N O S3123 1985 560 562 16
0 0
Molecule 41 is a protein called Eukaryotic translation initiation factor 2 subunit beta.
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Page 11 Full wwPDB EM Validation Report EMD-4327, 6FYX
Mol Chain Residues Atoms AltConf Trace
41 l 128Total C N O S1036 661 186 182 7
0 0
Molecule 42 is a protein called Eukaryotic translation initiation factor 5.
Mol Chain Residues Atoms AltConf Trace
42 m 147Total C N O S1140 724 201 208 7
0 0
Molecule 43 is a protein called Eukaryotic translation initiation factor 3 subunit A.
Mol Chain Residues Atoms AltConf Trace
43 o 529Total C N O S4070 2597 697 769 7
0 0
Molecule 44 is a protein called Eukaryotic translation initiation factor 3 subunit B.
Mol Chain Residues Atoms AltConf Trace
44 p 647Total C N O S5114 3274 880 942 18
0 0
Molecule 45 is a protein called eIF3c,eIF3c.
Mol Chain Residues Atoms AltConf Trace
45 q 621Total C N O S4827 3076 813 926 12
0 0
Molecule 46 is a protein called Eukaryotic translation initiation factor 3 subunit G.
Mol Chain Residues Atoms AltConf Trace
46 r 49Total C N O392 240 76 76
0 0
Molecule 47 is a protein called Eukaryotic translation initiation factor 3 subunit I.
Mol Chain Residues Atoms AltConf Trace
47 s 330Total C N O S2606 1661 429 507 9
0 0
Molecule 48 is MAGNESIUM ION (three-letter code: MG) (formula: Mg).
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Page 12 Full wwPDB EM Validation Report EMD-4327, 6FYX
Mol Chain Residues Atoms AltConf
48 2 116Total Mg116 116
0
48 k 1Total Mg1 1
0
Molecule 49 is ZINC ION (three-letter code: ZN) (formula: Zn).
Mol Chain Residues Atoms AltConf
49 b 1Total Zn1 1
0
49 a 1Total Zn1 1
0
49 l 1Total Zn1 1
0
49 f 1Total Zn1 1
0
49 m 1Total Zn1 1
0
Molecule 50 is METHIONINE (three-letter code: MET) (formula: C5H11NO2S).
Mol Chain Residues Atoms AltConf
50 k 1Total C N O S8 5 1 1 1
0
Molecule 51 is PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER (three-letter code: GCP) (formula: C11H18N5O13P3).
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Page 13 Full wwPDB EM Validation Report EMD-4327, 6FYX
Mol Chain Residues Atoms AltConf
51 k 1Total C N O P32 11 5 13 3
0
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Page 14 Full wwPDB EM Validation Report EMD-4327, 6FYX
3 Residue-property plots iO
These plots are drawn for all protein, RNA, DNA and oligosaccharide chains in the entry. The�rst graphic for a chain summarises the proportions of the various outlier classes displayed in thesecond graphic. The second graphic shows the sequence view annotated by issues in geometry andatom inclusion in map density. Residues are color-coded according to the number of geometricquality criteria for which they contain at least one outlier: green = 0, yellow = 1, orange = 2and red = 3 or more. A red diamond above a residue indicates a poor �t to the EM map forthis residue (all-atom inclusion < 40%). Stretches of 2 or more consecutive residues without anyoutlier are shown as a green connector. Residues present in the sample, but not in the model, areshown in grey.
• Molecule 1: tRNAi
Chain 1:
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• Molecule 2: 18S ribosomal RNA
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https://www.wwpdb.org/validation/2017/EMValidationReportHelp#residue_plots
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Page 15 Full wwPDB EM Validation Report EMD-4327, 6FYX
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A1699�
A1700�
C1701�
U1702�
C1703�
C1704�
A1705�
U1706�
C1707
U1708�
C1709
A1710�
G1711
A1712
G1715
A1719
G1725
A1729
G1734
A1742
G1743
A1748
C1749
U1750
A1753
A1754
G1755
G1758
U1759
A1760
A1763
A1764
G1765
G1766
U1767
U1768
U1769
G1778
A1779
A1780
C1781
G1787
A1788
A1789
G1790
G1791
A1792
U1793
C1794
A1795
U1796
U1797
A1798�
• Molecule 3: mRNA (31-MER)
Chain 3:
G G G C U C U C U U C U C U C15
�U16
�C17
�U18
�A19
�A20
�C21
�U22
�A23
�U24
�A25
A26
U32
�C33
�U34
�C35
�U36
�U37
�C38
�U39
�C40
�U41
�C42
�U43
�C44
�U45
�C G A U
• Molecule 4: 40S ribosomal protein S0
-
Page 16 Full wwPDB EM Validation Report EMD-4327, 6FYX
Chain A:
MET
S2
L3
T6
L17
L24
G25
A26
K27
N28
�
H32
�Q33
E34
R41
P42
D43
�
W54
R79
T80
V86
T93
R101�
F102
T103
S106
F107
T108
N109
Y110
I111
K116
L120
V121
R127
D150�
E154
D157
V158
R165
H168
L172
L176
L184
R185
L188
R191
W195
Y202
R205
N206
P207
E208�
E209�
Q212�
Q213�
T214�
A215�
E216�
E217�
E218�
A219�
V220�
ALA
SER
GLY
GLU
GLN
THR
GLU
GLU
ALA
VAL
ASP
ALA
THR
GLU
GLU
GLN
THR
GLU
ALA
ALA
GLU
TRP
ALA
GLU
GLU
GLY
GLN
ALA
GLN
GLU
GLU
GLU
TRP
ASN
• Molecule 5: 40S ribosomal protein S1
Chain B:
MET
A2
V3
�G4
�K5
�N6
�K7
�R8
�L9
�S10
LYS
GLY
LYS
LYS
GLY
LEU
LYS
K18
�R19
�V20
�
R26
G53
�L54
�K55
�
R64
E67
�
D72
E77
�
R82
D89
G93
M103
D104
F105
T106
T107
D108�
K109
L110
V114
L120
I121
E122�
A123
N124
V127
D131
I140
T143
Q146
A147
N148
T153
H160
S179
T180
L181
E191�
P206
L207
R213
P221
K222
F223�
D224�
L225�
G226�
S227�
L228
L229�
S230�
G233�
GLU
ALA
SER
ALA
GLU
GLU
LYS
GLY
LYS
LYS
VAL
ALA
GLY
PHE
LYS
ASP
GLU
ILE
LEU
GLU
THR
VAL
• Molecule 6: KLLA0F09812p
Chain C:
MET
SER
ALA
PRO
GLN
ALA
GLN
GLY
GLN
GLN
ALA
PRO
ARG
ARG
GLY
GLY
PHE
GLY
GLY
ALA
ASN
ARG
GLY
GLY
ARG
GLY
GLY
ARG
ARG
GLY
GLY
ARG
ARG
ASP
GLN
E36
�E37
�K38
�G39
W40
T44
I58
V68
K69
E70
F71
Q72
D83
M86
V91
Q94
T95
R100�
T101
R102
V109
V116
R131�
V141
R145
R146
T151
N152
L153
G154
Q155
L159
R173
S181
V198
T206
Y227
L230
N233
Q238
A239�
L240
T241
D250�
T253
A254�
S255�
LYS
LYS
LYS
LEU
• Molecule 7: KLLA0D08305p
Chain D:
MET
VAL
A3
�
L11
V12
A13
D14
�
E23
E31
�
E38
�
V41
�T42
P43
E47
E61
�N62
G63
�
I66
L69
T70
L71
K75
R76
Y79
K80
R81
�
L86
V91
H92
�
L110
R116
R117
Y120
E128
L142
R143�
M150�
V164
R173
L176
L177
R178�
I184
R194
S197�
G198�
D204�
E214�
L218�
E219�
P220
D224�
Y225
R226�
P227�
T228�
E229�
PRO
VAL
GLU
ALA
ALA
GLU
SER
ALA
• Molecule 8: 40S ribosomal protein S4
Chain E:
-
Page 17 Full wwPDB EM Validation Report EMD-4327, 6FYX
MET
A2
�
L9
W18
D21
L38
R39
L42
R49
N50
R51
N57
Q67
D73
R77
T80
T81
R100
K106
G107
R108
R113
I114
K133�
K155
D163
T166
I173
K174
F175�
V181
R187
I195
R198
E199
R200
G204
F205
D206
L207
V208
T220
R221
V225
L238
K245
R254
H259�
G260�
L261�
• Molecule 9: KLLA0D10659p
Chain F:
MET
SER
GLU
HIS
GLU
ALA
GLN
VAL
GLU
VAL
GLU
VAL
GLN
GLU
ASP
PHE
GLU
VAL
VAL
GLN
GLU
F22
�V23
�P24
�V25
�E26
�L27
�A28
�
V33
�E34
�I35
Q36
�Q37
�A38
�Q39
�
I42
K43
L44
K56
�D57
�
D62
�
Q65
I66
I70
Y71
V72
R78
V92
N102
G103�
R104
L110
E121
T127
Q133
D137�
R145
R150�
V151
G152�
G153�
G154�
G155�
A156�
A157�
R158
R159
Q160
D163
R168
R169
E183
R187
N188
I189
I192
L196
I213
L219
R227
• Molecule 10: 40S ribosomal protein S6
Chain G:
M1
C15
D19
�
R25
�V26
D29
�
E35
�
E39
�
G42
�D43
�
K46
�
I52
N56
H81
�
R92
D105�
V108
E122�
T125
R132
E150�
D151
D152
V153
R154
E161�
V162
T163�
K164�
G165�
D166�
K171�
R177
T180
Q199
R202
E203
R215
E222
K223�
A224�
E225�
V226�
R227�
K228�
R229�
R230�
ALA
SER
SER
LEU
LYS
ALA
• Molecule 11: 40S ribosomal protein S7
Chain H:
MET
SER
ASP
P4
K7
�
S10
�Q11
�
T14
E15
L16
�
Q19
F24
�
N29
�
P32
�E33
�L34
�K35
A36
�D37
�L38
R39
�
K44
�
R47
�
E50
�V51
A52
�G53
�G54
�
V60
V64
Y70
H71
Q74
E80
L81
E82
�K83
�
Q86
�D87
R88
L93
I98
K105�
S106
R107
Q108�
R114
S115
R116
T117
L118
H122
D123�
L126
E127
D128
R139
D158
V159
Q160�
D163�
N164
N174
K175
L176
S187�
GLU
THR
HIS
• Molecule 12: 40S ribosomal protein S8
Chain I:
MET
G2
R5
Q20
F21
R22
K23
K24
R25
E28
�L29
N35
T40
V46
N52
L58
R59
V72
R92
T93
N94
T95
L96
A99
S120�
L121�
G122
K123�
LYS
LYS
ASN
THR
LYS
ALA
GLU
GLU
GLU
THR
ALA
THR
T136�
S137�
K138�
N139�
T140�
E141�
R142�
K143�
-
Page 18 Full wwPDB EM Validation Report EMD-4327, 6FYX
W144
A145�
A146�
R147�
A148
A149�
I153
E154
D158
R179
R196
A199�
K200�
K201�
• Molecule 13: KLLA0E23673p
Chain J:
MET
P2
Y8
S9
K10
D25
L28
K37
N38
I45
L49
L60
E64
L70
L93
K100
D103
R107
R108
L109
L118
V127
Q131
I134
G137
F146
R149
L150
E151�
K154
R160�
T161
P169
G170
R171
A179�
A180�
G181�
G182�
E183�
GLU
ALA
ASP
GLU
GLU
• Molecule 14: KLLA0B08173p
Chain K:
M1
�
D7
�
Q13
H14
L15
L21
V22
A23
D26
�
E34
I35
D36
�
N39
L40
K44
L49
Y64
L76
�
H85
�I86
A89
�
L92
�A93
�G94
�Q95
�S96
�GLY
ASP
GLN
ARG
PRO
GLN
GLY
LYS
LYS
TYR
• Molecule 15: KLLA0A10483p
Chain L:
MET
S2
�T3
�E4
�L5
�T6
V7
E10
R11
�
K26
�A27
N28
�R29
�K30
�
K36
D55
K56
R67
G68
K69
R79
�
V111
R116�
V117
V125
T134
V135
R136
S144�
A145�
T146�
G147�
K148�
A149�
N150�
K151�
Q152�
F153�
A154�
K155�
F156�
• Molecule 16: 40S ribosomal protein S12
Chain M:
MET
SER
ASP
VAL
GLU
GLU
VAL
GLN
GLN
VAL
PRO
VAL
ALA
GLU
LEU
THR
I17
�E18
�D19
�A20
�L21
�K22
V23
�
R26
�T27
�S28
L29
�V30
�H31
�D32
�G33
�L34
�A35
�R36
�G37
L38
R39
E40
L45
�T46
R47
�G48
�E49
�G50
�Q51
�L52
A53
�V54
L55
V56
�E57
�S58
�V59
�T60
E61
�E62
�A63
I64
�
L67
V68
�
L71
�A72
T73
�E74
�ASN
N76
�V77
P78
L79
�I80
�K81
V82
A83
�D84
�A85
�K86
�Q87
�L88
G89
E90
�W91
A92
G93
�L94
G95
�K96
�I97
�D98
�R99
�D100�
G101
N102�
A103�
R104
K105�
V106�
A109�
S110�
K115�
N116�
W117
G118�
A119�
D120�
T121�
Q122�
E123�
R124
E125
I126�
L127
L128�
E129�
H130�
F131�
S132�
Q133�
Q134�
• Molecule 17: KLLA0F18040p
Chain N:
-
Page 19 Full wwPDB EM Validation Report EMD-4327, 6FYX
M1
�G2
�R3
K7
�G8
K9
R20
E83
�
L88
K107
D110�
L115
I116
R121
S143�
A144�
T145�
A148�
L149�
V150�
N151�
• Molecule 18: 40S ribosomal protein S14
Chain O:
MET
ALA
ASN
VAL
VAL
GLN
ALA
LYS
D9
�N10
�S11
�
F20
E37
A40
R41
V42
K49
�A50
D51
R52
Q65
K72
R84
A85
T86
S91
K92
T93
P94
G95
�
R114
D117�
D124
S125
T126
L137
• Molecule 19: KLLA0F07843p
Chain P:
MET
SER
GLU
ALA
ALA
ALA
PRO
ARG
LYS
ARG
SER
PHE
K13
�
Y17
L36
A37
R40
L49
�S50
E51
�K52
�
L56
K59
L60
E69
N70
�E71
�K72
H79
P87
E88
M89
V94
V105
M111
V126
R127�
H128
G129�
ARG
ALA
GLY
ALA
THR
THR
SER
ARG
PHE
ILE
PRO
LEU
ARG
• Molecule 20: 40S ribosomal protein S16
Chain Q:
MET
SER
T3
K26
Q40
�
L52
D58
�
V69
V78
R82
Q83
K87
G88
L89
Q94
V97
D113
L116
K127
R137
F138
R143
• Molecule 21: KLLA0B01474p
Chain R:
MET
G2
R3
V4
R5
R11
�
M26
�
Q29
D36
�
L46
T55
K63
�
V66
�
R78
�
K81
�D82
�
V88
�S89
A90
L91
D92
�
S96
�N97
�D98
V99
�L100
N101�
T106
L109
V110
N111�
L115�
K116�
L117
V124�
S125�
A126�
V127�
R128�
D129�
R130�
R131�
PHE
ARG
LYS
ARG
ASN
• Molecule 22: KLLA0B01562p
Chain S:
MET
S2
�L3
V4
�V5
Q6
�E7
�Q8
�G9
�
L15
R41
D51
�
R57
L61
M73
F85
L86
N87
D91
V92
D97
L105
E106
S107
K108
L109
R110
D111�
E114
R120�
R123�
F128�
R134
G135
Q136�
H137
T141�
G142�
R143
R144�
R145�
A146�
• Molecule 23: KLLA0A07194p
Chain T:
-
Page 20 Full wwPDB EM Validation Report EMD-4327, 6FYX
MET
P2
�
D8
N16
N17
Q23
L28
Q48
I65
R68
L79
R86
G87
V88
K110
L111
I124
S125
D126
L129
D133
L140
E141�
D142�
E143�
E144�
• Molecule 24: KLLA0F25542p
Chain U:
MET
SER
GLN
VAL
GLU
LYS
LYS
SER
GLU
GLN
GLN
Q15
�E16
�V17
�V18
�I19
�
T27
V31
K32
�
E46
�
K49
�
K52
V56
R57
I65
E74
K77
I86
R89
E94
�A95
P96
A97
H98
�
R102�
T107�
I108
E109�
P110�
D113�
V114
E115�
I118�
A119�
A120�
• Molecule 25: 40S ribosomal protein S21
Chain V:
M1
L11
Y12
S30
Y50
E64
�A65
D66
�
D76
K80
�
R87
�
• Molecule 26: 40S ribosomal protein S22
Chain W:
MET
T2
Q24
V25
R28
P29
I47
S58
C72
P77
R78
D85
�
K88
L104
V129
Y130
• Molecule 27: KLLA0B11231p
Chain X:
MET
G2
K3
G4
K14
L15
R16
L34
�
S41
I59
Q63
V72
N79
D90
N94
F107
V127
E138
P143�
R144�
S145�
• Molecule 28: 40S ribosomal protein S24
Chain Y:
MET
S2
�D3
�A4
R20
V35
S36
L74
K84
E98
�
E101�
R105
R111
T121
I125�
A129�
R132
N133�
A134�
D135�
• Molecule 29: KLLA0B06182p
Chain Z:
-
Page 21 Full wwPDB EM Validation Report EMD-4327, 6FYX
MET
PRO
PRO
LYS
GLN
GLN
LEU
SER
LYS
ALA
ALA
LYS
ALA
ALA
ALA
ALA
MET
ALA
GLY
GLY
LYS
LYS
SER
LYS
LYS
LYS
TRP
S28
�K29
�K30
�S31
�H32
�K33
�D34
�K35
�A36
�K37
�H38
A39
�V40
�V41
L42
�D43
�Q44
D45
�K46
�
I50
�M51
K52
�E53
�A54
�P55
T56
�
D67
�R68
�F69
�
L75
A79
�L80
R81
�H82
�L83
E84
�N85
�E86
�G87
�I88
I89
�
S93
�K94
�
K97
�Q98
�A99
�
R103�
A104�
T105�
ALA
SER
GLU
• Molecule 30: 40S ribosomal protein S26
Chain a:
MET
P2
K12
K13
D33
�
R38
M39
A48
�
Y59
L64
I75
V84
R89
�
R93
�
Q99
�R100�
P101�
R102�
F103�
N104�
ARG
ASP
ASN
LYS
VAL
SER
PRO
ALA
ASP
ALA
ALA
LYS
LYS
ALA
LEU
• Molecule 31: 40S ribosomal protein S27
Chain b:
MET
V2
L3
E15
�
L21
K22
T23
L24
H31
P38
�G39
�
T52
E57
�
S60
�T61
V62
E75
�
K82
• Molecule 32: 40S ribosomal protein S28
Chain c:
MET
ASP
THR
K4
�T5
�P6
�
K14
V15
L16
D35
R38
�
P47
V48
R49
E50
M57
�E58
S59
E60
�
R64
�R65
�L66
R67
�
• Molecule 33: 40S ribosomal protein S29
Chain d:
MET
A2
�H3
E4
�
K16
R19
L30
N37
I38
D39
R40
Q41
R56
• Molecule 34: 40S ribosomal protein S30
Chain e:
MET
G2
�K3
�V4
H5
�
L8
A11
Q17
Q24
E25
K26
K36
V47
T48
�L49
�T50
�N51
�G52
�K53
�R54
�K55
S61
�SER
GLN
• Molecule 35: Ubiquitin-40S ribosomal protein S27a
Chain f:
-
Page 22 Full wwPDB EM Validation Report EMD-4327, 6FYX
MET
GLN
ILE
PHE
VAL
LYS
THR
LEU
THR
GLY
LYS
THR
ILE
THR
LEU
GLU
VAL
GLU
SER
SER
ASP
THR
ILE
ASP
ASN
VAL
LYS
SER
LYS
ILE
GLN
ASP
LYS
GLU
GLY
ILE
PRO
PRO
ASP
GLN
GLN
ARG
LEU
ILE
PHE
ALA
GLY
LYS
GLN
LEU
GLU
ASP
GLY
ARG
THR
LEU
SER
ASP
TYR
ASN
ILE
GLN
LYS
GLU
SER
THR
LEU
HIS
LEU
VAL
LEU
ARG
LEU
ARG
GLY
GLY
G77
�K78
�K79
�R80
�K81
�K82
�K83
�V84
�Y85
�T86
�T87
�P88
�K89
K90
�I91
R92
H93
K94
�
L100�
A101
V102
L103�
Y106�
K107
V108�
D109�
D110�
E111�
G112�
K113�
V114�
A115
K116�
L117
R118�
K119
E120�
C124�
G125�
P126
G127�
D135�
R136
C142�
A147�
T148�
Q149�
K150�
• Molecule 36: KLLA0E12277p
Chain g:
MET
SER
S3
�S4
�N5
�I6
�M7
L8
R11
�
E15
�G16
A28
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• Molecule 37: 60S ribosomal protein L41-A
Chain h:
M1
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A3
�
R6
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R9
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R11
�R12
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• Molecule 38: Eukaryotic translation initiation factor 1A
Chain i:
MET
G2
�K3
�K4
�N5
�T6
�K7
�G8
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R13
R14
G15
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ASP
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GLU
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GLU
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ASP
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ASP
ASP
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• Molecule 39: Eukaryotic translation initiation factor 2 subunit alpha
Chain j:
MET
SER
T3
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R7
F8
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E10
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K61
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�
-
Page 23 Full wwPDB EM Validation Report EMD-4327, 6FYX
V70
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LEU
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SER
ASP
SER
GLU
ASP
ASP
GLU
ASP
GLU
SER
ASP
ASP
GLU
• Molecule 40: Eukaryotic translation initiation factor 2 subunit gamma
Chain k:
MET
SER
ASP
LEU
GLN
ASP
GLN
GLU
PRO
SER
ILE
ILE
ILE
ASN
GLY
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-
Page 24 Full wwPDB EM Validation Report EMD-4327, 6FYX
• Molecule 41: Eukaryotic translation initiation factor 2 subunit beta
Chain l:
MET
SER
SER
ASP
LEU
ALA
ALA
GLU
LEU
GLY
PHE
ASP
PRO
ALA
LEU
LYS
LYS
LYS
LYS
LYS
THR
LYS
LYS
VAL
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ASP
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VAL
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LYS
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VAL
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ALA
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ALA
GLU
LYS
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ASP
ILE
ALA
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ALA
LEU
GLY
GLU
LEU
SER
LEU
LYS
LYS
LYS
LYS
LYS
LYS
THR
LYS
ASP
SER
SER
VAL
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ALA
PHE
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LYS
GLU
LEU
ALA
LYS
ALA
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VAL
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GLU
SER
LYS
GLU
GLY
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PRO
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ALA
ASN
SER
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GLN
GLN
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V127�
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ARG
MET
• Molecule 42: Eukaryotic translation initiation factor 5
Chain m:
MET
SER
I3
�N4
�I5
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R7
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LYS
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ALA
ALA
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THR
LEU
GLU
ASP
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GLU
VAL
LYS
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ASP
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LYS
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ILE
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LYS
LYS
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VAL
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LYS
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VAL
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LYS
LYS
VAL
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ARG
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ALA
LYS
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ILE
THR
TRP
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GLU
THR
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GLU
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ASP
ASP
ASP
GLU
GLU
ASP
ASP
GLU
• Molecule 43: Eukaryotic translation initiation factor 3 subunit A
Chain o:
-
Page 25 Full wwPDB EM Validation Report EMD-4327, 6FYX
MET
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-
Page 26 Full wwPDB EM Validation Report EMD-4327, 6FYX
MET
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• Molecule 44: Eukaryotic translation initiation factor 3 subunit B
Chain p:
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-
Page 27 Full wwPDB EM Validation Report EMD-4327, 6FYX
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• Molecule 45: eIF3c,eIF3c
Chain q:
UNK
UNK
UNK
UNK
UNK
UNK
UNK
UNK
UNK
UNK
UNK
UNK
UNK
UNK
UNK
UNK
UNK
UNK
UNK
UNK
UNK
UNK
UNK
UNK
UNK
UNK
UNK
UNK
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