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  • Full wwPDB EM Validation Report iO

    Dec 6, 2020 � 04:47 am GMT

    PDB ID : 6FYXEMDB ID : EMD-4327

    Title : Structure of a partial yeast 48S preinitiation complex with eIF5 N-terminaldomain (model C1)

    Authors : Llacer, J.L.; Hussain, T.; Gordiyenko, Y.; Ramakrishnan, V.Deposited on : 2018-03-12Resolution : 3.05 Å(reported)

    This is a Full wwPDB EM Validation Report for a publicly released PDB entry.

    We welcome your comments at [email protected] user guide is available at

    https://www.wwpdb.org/validation/2017/EMValidationReportHelpwith speci�c help available everywhere you see the iO symbol.

    The following versions of software and data (see references iO) were used in the production of this report:

    EMDB validation analysis : 0.0.0.dev61Mogul : 1.8.5 (274361), CSD as541be (2020)

    MolProbity : 4.02b-467buster-report : 1.1.7 (2018)

    Percentile statistics : 20191225.v01 (using entries in the PDB archive December 25th 2019)Ideal geometry (proteins) : Engh & Huber (2001)

    Ideal geometry (DNA, RNA) : Parkinson et al. (1996)Validation Pipeline (wwPDB-VP) : 2.15.1

    https://www.wwpdb.org/validation/2017/EMValidationReportHelphttps://www.wwpdb.org/validation/2017/EMValidationReportHelphttps://www.wwpdb.org/validation/2017/EMValidationReportHelphttps://www.wwpdb.org/validation/2017/EMValidationReportHelp#references

  • Page 2 Full wwPDB EM Validation Report EMD-4327, 6FYX

    1 Overall quality at a glance iO

    The following experimental techniques were used to determine the structure:ELECTRON MICROSCOPY

    The reported resolution of this entry is 3.05 Å.

    Percentile scores (ranging between 0-100) for global validation metrics of the entry are shown inthe following graphic. The table shows the number of entries on which the scores are based.

    MetricWhole archive(#Entries)

    EM structures(#Entries)

    Ramachandran outliers 154571 4023Sidechain outliers 154315 3826RNA backbone 4643 859

    The table below summarises the geometric issues observed across the polymeric chains and their �tto the map. The red, orange, yellow and green segments of the bar indicate the fraction of residuesthat contain outliers for >=3, 2, 1 and 0 types of geometric quality criteria respectively. A greysegment represents the fraction of residues that are not modelled. The numeric value for eachfraction is indicated below the corresponding segment, with a dot representing fractions

  • Page 3 Full wwPDB EM Validation Report EMD-4327, 6FYX

    Continued from previous page...

    Mol Chain Length Quality of chain

    9 F 227

    10 G 236

    11 H 190

    12 I 201

    13 J 188

    14 K 106

    15 L 156

    16 M 134

    17 N 151

    18 O 137

    19 P 142

    20 Q 143

    21 R 136

    22 S 146

    23 T 144

    24 U 117

    25 V 87

    26 W 130

    27 X 145

    28 Y 135

    29 Z 108

    30 a 119

    31 b 82

    32 c 67

    33 d 56Continued on next page...

  • Page 4 Full wwPDB EM Validation Report EMD-4327, 6FYX

    Continued from previous page...

    Mol Chain Length Quality of chain

    34 e 63

    35 f 150

    36 g 326

    37 h 25

    38 i 153

    39 j 304

    40 k 527

    41 l 285

    42 m 405

    43 o 964

    44 p 763

    45 q 812

    46 r 274

    47 s 347

    The following table lists non-polymeric compounds, carbohydrate monomers and non-standardresidues in protein, DNA, RNA chains that are outliers for geometric or electron-density-�t crite-ria:

    Mol Type Chain Res Chirality Geometry Clashes Electron density2 C4J 2 1190 X - - -

  • Page 5 Full wwPDB EM Validation Report EMD-4327, 6FYX

    2 Entry composition iO

    There are 51 unique types of molecules in this entry. The entry contains 104404 atoms, of which0 are hydrogens and 0 are deuteriums.

    In the tables below, the AltConf column contains the number of residues with at least one atomin alternate conformation and the Trace column contains the number of residues modelled with atmost 2 atoms.

    Molecule 1 is a RNA chain called tRNAi.

    Mol Chain Residues Atoms AltConf Trace

    1 1 75Total C N O P1639 734 298 531 76

    0 0

    Molecule 2 is a RNA chain called 18S ribosomal RNA.

    Mol Chain Residues Atoms AltConf Trace

    2 2 1780Total C N O P37812 16904 6659 12469 1780

    0 0

    There are 2 discrepancies between the modelled and reference sequences:

    Chain Residue Modelled Actual Comment Reference2 676 G U con�ict GB 496422082 678 U G con�ict GB 49642208

    Molecule 3 is a RNA chain called mRNA (31-MER).

    Mol Chain Residues Atoms AltConf Trace

    3 3 31Total C N O P719 324 108 252 35

    4 0

    Molecule 4 is a protein called 40S ribosomal protein S0.

    Mol Chain Residues Atoms AltConf Trace

    4 A 219Total C N O S1702 1085 299 316 2

    0 0

    Molecule 5 is a protein called 40S ribosomal protein S1.

    Mol Chain Residues Atoms AltConf Trace

    5 B 225Total C N O S1797 1135 330 329 3

    0 0

    https://www.wwpdb.org/validation/2017/EMValidationReportHelp#entry_composition

  • Page 6 Full wwPDB EM Validation Report EMD-4327, 6FYX

    Molecule 6 is a protein called KLLA0F09812p.

    Mol Chain Residues Atoms AltConf Trace

    6 C 220Total C N O S1648 1053 291 300 4

    0 0

    Molecule 7 is a protein called KLLA0D08305p.

    Mol Chain Residues Atoms AltConf Trace

    7 D 227Total C N O S1774 1126 320 323 5

    0 0

    Molecule 8 is a protein called 40S ribosomal protein S4.

    Mol Chain Residues Atoms AltConf Trace

    8 E 260Total C N O S2078 1322 393 359 4

    0 0

    Molecule 9 is a protein called KLLA0D10659p.

    Mol Chain Residues Atoms AltConf Trace

    9 F 206Total C N O S1609 1008 298 300 3

    0 0

    Molecule 10 is a protein called 40S ribosomal protein S6.

    Mol Chain Residues Atoms AltConf Trace

    10 G 230Total C N O S1832 1146 352 330 4

    0 0

    Molecule 11 is a protein called 40S ribosomal protein S7.

    Mol Chain Residues Atoms AltConf Trace

    11 H 184Total C N O1483 950 270 263

    0 0

    Molecule 12 is a protein called 40S ribosomal protein S8.

    Mol Chain Residues Atoms AltConf Trace

    12 I 188Total C N O S1489 923 300 265 1

    0 0

    Molecule 13 is a protein called KLLA0E23673p.

  • Page 7 Full wwPDB EM Validation Report EMD-4327, 6FYX

    Mol Chain Residues Atoms AltConf Trace

    13 J 182Total C N O S1471 929 287 254 1

    0 0

    Molecule 14 is a protein called KLLA0B08173p.

    Mol Chain Residues Atoms AltConf Trace

    14 K 96Total C N O S809 533 129 146 1

    0 0

    Molecule 15 is a protein called KLLA0A10483p.

    Mol Chain Residues Atoms AltConf Trace

    15 L 155Total C N O S1248 798 237 210 3

    0 0

    Molecule 16 is a protein called 40S ribosomal protein S12.

    Mol Chain Residues Atoms AltConf Trace

    16 M 117Total C N O885 553 161 171

    0 0

    Molecule 17 is a protein called KLLA0F18040p.

    Mol Chain Residues Atoms AltConf Trace

    17 N 151Total C N O S1195 761 224 207 3

    0 0

    Molecule 18 is a protein called 40S ribosomal protein S14.

    Mol Chain Residues Atoms AltConf Trace

    18 O 129Total C N O S954 584 191 176 3

    0 0

    Molecule 19 is a protein called KLLA0F07843p.

    Mol Chain Residues Atoms AltConf Trace

    19 P 117Total C N O S923 592 165 161 5

    0 0

    Molecule 20 is a protein called 40S ribosomal protein S16.

  • Page 8 Full wwPDB EM Validation Report EMD-4327, 6FYX

    Mol Chain Residues Atoms AltConf Trace

    20 Q 141Total C N O1105 709 204 192

    0 0

    Molecule 21 is a protein called KLLA0B01474p.

    Mol Chain Residues Atoms AltConf Trace

    21 R 130Total C N O S1033 643 194 193 3

    0 0

    Molecule 22 is a protein called KLLA0B01562p.

    Mol Chain Residues Atoms AltConf Trace

    22 S 145Total C N O S1189 739 239 209 2

    0 0

    Molecule 23 is a protein called KLLA0A07194p.

    Mol Chain Residues Atoms AltConf Trace

    23 T 143Total C N O1110 693 210 207

    0 0

    Molecule 24 is a protein called KLLA0F25542p.

    Mol Chain Residues Atoms AltConf Trace

    24 U 106Total C N O S845 540 152 152 1

    0 0

    Molecule 25 is a protein called 40S ribosomal protein S21.

    Mol Chain Residues Atoms AltConf Trace

    25 V 87Total C N O S687 424 126 135 2

    0 0

    Molecule 26 is a protein called 40S ribosomal protein S22.

    Mol Chain Residues Atoms AltConf Trace

    26 W 129Total C N O S1021 651 187 180 3

    0 0

    Molecule 27 is a protein called KLLA0B11231p.

  • Page 9 Full wwPDB EM Validation Report EMD-4327, 6FYX

    Mol Chain Residues Atoms AltConf Trace

    27 X 144Total C N O S1119 708 218 191 2

    0 0

    Molecule 28 is a protein called 40S ribosomal protein S24.

    Mol Chain Residues Atoms AltConf Trace

    28 Y 134Total C N O1061 665 207 189

    0 0

    Molecule 29 is a protein called KLLA0B06182p.

    Mol Chain Residues Atoms AltConf Trace

    29 Z 78Total C N O S594 376 111 106 1

    0 0

    Molecule 30 is a protein called 40S ribosomal protein S26.

    Mol Chain Residues Atoms AltConf Trace

    30 a 103Total C N O S812 500 173 134 5

    0 0

    Molecule 31 is a protein called 40S ribosomal protein S27.

    Mol Chain Residues Atoms AltConf Trace

    31 b 81Total C N O S609 379 112 113 5

    0 0

    Molecule 32 is a protein called 40S ribosomal protein S28.

    Mol Chain Residues Atoms AltConf Trace

    32 c 64Total C N O S499 308 99 91 1

    0 0

    Molecule 33 is a protein called 40S ribosomal protein S29.

    Mol Chain Residues Atoms AltConf Trace

    33 d 55Total C N O S461 289 93 78 1

    0 0

    Molecule 34 is a protein called 40S ribosomal protein S30.

  • Page 10 Full wwPDB EM Validation Report EMD-4327, 6FYX

    Mol Chain Residues Atoms AltConf Trace

    34 e 60Total C N O S472 295 96 80 1

    0 0

    Molecule 35 is a protein called Ubiquitin-40S ribosomal protein S27a.

    Mol Chain Residues Atoms AltConf Trace

    35 f 74Total C N O S584 374 111 95 4

    0 0

    Molecule 36 is a protein called KLLA0E12277p.

    Mol Chain Residues Atoms AltConf Trace

    36 g 320Total C N O S2469 1561 432 471 5

    0 0

    Molecule 37 is a protein called 60S ribosomal protein L41-A.

    Mol Chain Residues Atoms AltConf Trace

    37 h 25Total C N O S233 142 63 27 1

    0 0

    Molecule 38 is a protein called Eukaryotic translation initiation factor 1A.

    Mol Chain Residues Atoms AltConf Trace

    38 i 121Total C N O S958 587 183 183 5

    0 0

    Molecule 39 is a protein called Eukaryotic translation initiation factor 2 subunit alpha.

    Mol Chain Residues Atoms AltConf Trace

    39 j 249Total C N O S2006 1283 333 382 8

    0 0

    Molecule 40 is a protein called Eukaryotic translation initiation factor 2 subunit gamma.

    Mol Chain Residues Atoms AltConf Trace

    40 k 414Total C N O S3123 1985 560 562 16

    0 0

    Molecule 41 is a protein called Eukaryotic translation initiation factor 2 subunit beta.

  • Page 11 Full wwPDB EM Validation Report EMD-4327, 6FYX

    Mol Chain Residues Atoms AltConf Trace

    41 l 128Total C N O S1036 661 186 182 7

    0 0

    Molecule 42 is a protein called Eukaryotic translation initiation factor 5.

    Mol Chain Residues Atoms AltConf Trace

    42 m 147Total C N O S1140 724 201 208 7

    0 0

    Molecule 43 is a protein called Eukaryotic translation initiation factor 3 subunit A.

    Mol Chain Residues Atoms AltConf Trace

    43 o 529Total C N O S4070 2597 697 769 7

    0 0

    Molecule 44 is a protein called Eukaryotic translation initiation factor 3 subunit B.

    Mol Chain Residues Atoms AltConf Trace

    44 p 647Total C N O S5114 3274 880 942 18

    0 0

    Molecule 45 is a protein called eIF3c,eIF3c.

    Mol Chain Residues Atoms AltConf Trace

    45 q 621Total C N O S4827 3076 813 926 12

    0 0

    Molecule 46 is a protein called Eukaryotic translation initiation factor 3 subunit G.

    Mol Chain Residues Atoms AltConf Trace

    46 r 49Total C N O392 240 76 76

    0 0

    Molecule 47 is a protein called Eukaryotic translation initiation factor 3 subunit I.

    Mol Chain Residues Atoms AltConf Trace

    47 s 330Total C N O S2606 1661 429 507 9

    0 0

    Molecule 48 is MAGNESIUM ION (three-letter code: MG) (formula: Mg).

  • Page 12 Full wwPDB EM Validation Report EMD-4327, 6FYX

    Mol Chain Residues Atoms AltConf

    48 2 116Total Mg116 116

    0

    48 k 1Total Mg1 1

    0

    Molecule 49 is ZINC ION (three-letter code: ZN) (formula: Zn).

    Mol Chain Residues Atoms AltConf

    49 b 1Total Zn1 1

    0

    49 a 1Total Zn1 1

    0

    49 l 1Total Zn1 1

    0

    49 f 1Total Zn1 1

    0

    49 m 1Total Zn1 1

    0

    Molecule 50 is METHIONINE (three-letter code: MET) (formula: C5H11NO2S).

    Mol Chain Residues Atoms AltConf

    50 k 1Total C N O S8 5 1 1 1

    0

    Molecule 51 is PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER (three-letter code: GCP) (formula: C11H18N5O13P3).

  • Page 13 Full wwPDB EM Validation Report EMD-4327, 6FYX

    Mol Chain Residues Atoms AltConf

    51 k 1Total C N O P32 11 5 13 3

    0

  • Page 14 Full wwPDB EM Validation Report EMD-4327, 6FYX

    3 Residue-property plots iO

    These plots are drawn for all protein, RNA, DNA and oligosaccharide chains in the entry. The�rst graphic for a chain summarises the proportions of the various outlier classes displayed in thesecond graphic. The second graphic shows the sequence view annotated by issues in geometry andatom inclusion in map density. Residues are color-coded according to the number of geometricquality criteria for which they contain at least one outlier: green = 0, yellow = 1, orange = 2and red = 3 or more. A red diamond above a residue indicates a poor �t to the EM map forthis residue (all-atom inclusion < 40%). Stretches of 2 or more consecutive residues without anyoutlier are shown as a green connector. Residues present in the sample, but not in the model, areshown in grey.

    • Molecule 1: tRNAi

    Chain 1:

    A1

    G7

    U8

    G9

    G10

    G15

    U16

    �G18

    G19

    A20

    �A21

    G22

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    G26

    A37

    C40

    G43

    A44

    U45

    �G46

    U47

    �C48

    C49

    G52

    A58

    A59

    A60

    C61

    A64

    �G65

    C66

    �G67

    �G68

    C69

    �G70

    �C71

    �U72

    �A73

    �C74

    �C75

    �A76

    • Molecule 2: 18S ribosomal RNA

    Chain 2:

    U1

    �A2

    U3

    C4

    U5

    C13

    G16

    C17

    C25

    A26

    U27

    G34

    G42

    A43

    U44

    U45

    A46

    A47

    G57

    U58

    C59

    U60

    A61

    U64

    A65

    U66

    A67

    A68

    G69

    A72

    U73

    �U74

    �U75

    �A76

    �U77

    �A78

    C79

    U101

    A104

    U111

    C114

    G115

    U116

    U120

    G123

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    G127

    U128

    U129

    C130�

    C131�

    U132�

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    A138

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    C159

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    A256

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    G321

    U331

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    A351

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    A399

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    A411

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    A415

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    U438

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    A533

    A534

    C535

    G536

    A537

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    A540

    A541

    C542

    A543

    A544

    C545

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    G547

    G548

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    C558

    C564

    G567

    G570

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    C582

    C583

    A584

    A593

    G594

    U597

    A605

    G609

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    A614

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    U617

    A618

    A619

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    G623

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    C G A C U U U A U G U C G C G C A C G676�

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    https://www.wwpdb.org/validation/2017/EMValidationReportHelp#residue_plots

  • Page 15 Full wwPDB EM Validation Report EMD-4327, 6FYX

    G711�

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    A991

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    G1007

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    C1009

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    U1011

    C1015

    A1018

    A1019

    C1020

    A1024

    A1025

    A1026

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    U1030

    G1031

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    A1255

    U1256

    G1260

    U1261

    G1262

    U1265

    U1268

    G1269

    G1272

    C1273

    A1274

    U1275

    C1283

    U1284

    G1287

    U1288

    U1289

    G1296

    U1306

    A1311

    A1312

    U1313

    U1314

    G1315

    C1316

    G1317

    A1320

    A1321

    A1324

    A1336

    C1337

    C1338

    U1339

    A1343

    A1344

    A1347

    G1353

    C1354

    U1355

    C1360

    U1361�

    U1362�

    G1363

    C1364

    C1365

    G1366

    U1369

    G1370

    A1380

    G1383

    A1386

    C1387

    U1388

    A1389

    U1390

    G1393

    U1396

    C1397

    A1398

    A1399

    G1400

    A1408

    A1409

    G1410

    U1411

    U1412

    U1413

    G1414

    A1415

    G1416

    G1417

    C1418

    A1423

    C1424

    A1425

    G1426

    G1427

    U1428

    C1429

    U1430

    G1431

    A1442

    G1443

    A1444

    C1455

    G1456

    C1457

    A1458

    C1459

    G1460

    G1464

    A1467

    C1468

    A1469

    C1470

    U1471

    G1472

    G1476

    C1479

    C1480

    A1481

    G1482

    C1483

    G1484

    A1488

    C1489�

    A1490

    A1491

    U1494

    G1504

    G1510

    G1511

    U1512

    A1513

    A1514

    U1515

    G1519

    U1520

    G1521

    A1522

    G1532

    U1533

    G1534

    C1535

    U1536

    G1537

    G1538

    G1539

    G1540

    U1552

    A1553

    A1554

    U1555

    U1556

    A1557

    U1558

    C1566

    G1570

    A1571

    G1572

    G1573

    A1581

    G1588

    A1595

    U1596

    C1597

    A1598

    G1599

    G1605

    C1613

    G1614

    C1617

    G1620

    A1629

    C1632

    A1633

    C1634

    C1637

    C1644

    G1647

    U1648

    U1655�

    G1656

    C1662

    U1667

    A1676

    G1677

    G1678

    A1679

    U1680

    U1681

    U1682

    U1685

    U1686�

    A1687

    G1688

    A1689�

    G1690�

    A1691�

    A1692�

    G1693�

    G1694�

    G1695�

    G1696�

    G1697�

    C1698�

    A1699�

    A1700�

    C1701�

    U1702�

    C1703�

    C1704�

    A1705�

    U1706�

    C1707

    U1708�

    C1709

    A1710�

    G1711

    A1712

    G1715

    A1719

    G1725

    A1729

    G1734

    A1742

    G1743

    A1748

    C1749

    U1750

    A1753

    A1754

    G1755

    G1758

    U1759

    A1760

    A1763

    A1764

    G1765

    G1766

    U1767

    U1768

    U1769

    G1778

    A1779

    A1780

    C1781

    G1787

    A1788

    A1789

    G1790

    G1791

    A1792

    U1793

    C1794

    A1795

    U1796

    U1797

    A1798�

    • Molecule 3: mRNA (31-MER)

    Chain 3:

    G G G C U C U C U U C U C U C15

    �U16

    �C17

    �U18

    �A19

    �A20

    �C21

    �U22

    �A23

    �U24

    �A25

    A26

    U32

    �C33

    �U34

    �C35

    �U36

    �U37

    �C38

    �U39

    �C40

    �U41

    �C42

    �U43

    �C44

    �U45

    �C G A U

    • Molecule 4: 40S ribosomal protein S0

  • Page 16 Full wwPDB EM Validation Report EMD-4327, 6FYX

    Chain A:

    MET

    S2

    L3

    T6

    L17

    L24

    G25

    A26

    K27

    N28

    H32

    �Q33

    E34

    R41

    P42

    D43

    W54

    R79

    T80

    V86

    T93

    R101�

    F102

    T103

    S106

    F107

    T108

    N109

    Y110

    I111

    K116

    L120

    V121

    R127

    D150�

    E154

    D157

    V158

    R165

    H168

    L172

    L176

    L184

    R185

    L188

    R191

    W195

    Y202

    R205

    N206

    P207

    E208�

    E209�

    Q212�

    Q213�

    T214�

    A215�

    E216�

    E217�

    E218�

    A219�

    V220�

    ALA

    SER

    GLY

    GLU

    GLN

    THR

    GLU

    GLU

    ALA

    VAL

    ASP

    ALA

    THR

    GLU

    GLU

    GLN

    THR

    GLU

    ALA

    ALA

    GLU

    TRP

    ALA

    GLU

    GLU

    GLY

    GLN

    ALA

    GLN

    GLU

    GLU

    GLU

    TRP

    ASN

    • Molecule 5: 40S ribosomal protein S1

    Chain B:

    MET

    A2

    V3

    �G4

    �K5

    �N6

    �K7

    �R8

    �L9

    �S10

    LYS

    GLY

    LYS

    LYS

    GLY

    LEU

    LYS

    K18

    �R19

    �V20

    R26

    G53

    �L54

    �K55

    R64

    E67

    D72

    E77

    R82

    D89

    G93

    M103

    D104

    F105

    T106

    T107

    D108�

    K109

    L110

    V114

    L120

    I121

    E122�

    A123

    N124

    V127

    D131

    I140

    T143

    Q146

    A147

    N148

    T153

    H160

    S179

    T180

    L181

    E191�

    P206

    L207

    R213

    P221

    K222

    F223�

    D224�

    L225�

    G226�

    S227�

    L228

    L229�

    S230�

    G233�

    GLU

    ALA

    SER

    ALA

    GLU

    GLU

    LYS

    GLY

    LYS

    LYS

    VAL

    ALA

    GLY

    PHE

    LYS

    ASP

    GLU

    ILE

    LEU

    GLU

    THR

    VAL

    • Molecule 6: KLLA0F09812p

    Chain C:

    MET

    SER

    ALA

    PRO

    GLN

    ALA

    GLN

    GLY

    GLN

    GLN

    ALA

    PRO

    ARG

    ARG

    GLY

    GLY

    PHE

    GLY

    GLY

    ALA

    ASN

    ARG

    GLY

    GLY

    ARG

    GLY

    GLY

    ARG

    ARG

    GLY

    GLY

    ARG

    ARG

    ASP

    GLN

    E36

    �E37

    �K38

    �G39

    W40

    T44

    I58

    V68

    K69

    E70

    F71

    Q72

    D83

    M86

    V91

    Q94

    T95

    R100�

    T101

    R102

    V109

    V116

    R131�

    V141

    R145

    R146

    T151

    N152

    L153

    G154

    Q155

    L159

    R173

    S181

    V198

    T206

    Y227

    L230

    N233

    Q238

    A239�

    L240

    T241

    D250�

    T253

    A254�

    S255�

    LYS

    LYS

    LYS

    LEU

    • Molecule 7: KLLA0D08305p

    Chain D:

    MET

    VAL

    A3

    L11

    V12

    A13

    D14

    E23

    E31

    E38

    V41

    �T42

    P43

    E47

    E61

    �N62

    G63

    I66

    L69

    T70

    L71

    K75

    R76

    Y79

    K80

    R81

    L86

    V91

    H92

    L110

    R116

    R117

    Y120

    E128

    L142

    R143�

    M150�

    V164

    R173

    L176

    L177

    R178�

    I184

    R194

    S197�

    G198�

    D204�

    E214�

    L218�

    E219�

    P220

    D224�

    Y225

    R226�

    P227�

    T228�

    E229�

    PRO

    VAL

    GLU

    ALA

    ALA

    GLU

    SER

    ALA

    • Molecule 8: 40S ribosomal protein S4

    Chain E:

  • Page 17 Full wwPDB EM Validation Report EMD-4327, 6FYX

    MET

    A2

    L9

    W18

    D21

    L38

    R39

    L42

    R49

    N50

    R51

    N57

    Q67

    D73

    R77

    T80

    T81

    R100

    K106

    G107

    R108

    R113

    I114

    K133�

    K155

    D163

    T166

    I173

    K174

    F175�

    V181

    R187

    I195

    R198

    E199

    R200

    G204

    F205

    D206

    L207

    V208

    T220

    R221

    V225

    L238

    K245

    R254

    H259�

    G260�

    L261�

    • Molecule 9: KLLA0D10659p

    Chain F:

    MET

    SER

    GLU

    HIS

    GLU

    ALA

    GLN

    VAL

    GLU

    VAL

    GLU

    VAL

    GLN

    GLU

    ASP

    PHE

    GLU

    VAL

    VAL

    GLN

    GLU

    F22

    �V23

    �P24

    �V25

    �E26

    �L27

    �A28

    V33

    �E34

    �I35

    Q36

    �Q37

    �A38

    �Q39

    I42

    K43

    L44

    K56

    �D57

    D62

    Q65

    I66

    I70

    Y71

    V72

    R78

    V92

    N102

    G103�

    R104

    L110

    E121

    T127

    Q133

    D137�

    R145

    R150�

    V151

    G152�

    G153�

    G154�

    G155�

    A156�

    A157�

    R158

    R159

    Q160

    D163

    R168

    R169

    E183

    R187

    N188

    I189

    I192

    L196

    I213

    L219

    R227

    • Molecule 10: 40S ribosomal protein S6

    Chain G:

    M1

    C15

    D19

    R25

    �V26

    D29

    E35

    E39

    G42

    �D43

    K46

    I52

    N56

    H81

    R92

    D105�

    V108

    E122�

    T125

    R132

    E150�

    D151

    D152

    V153

    R154

    E161�

    V162

    T163�

    K164�

    G165�

    D166�

    K171�

    R177

    T180

    Q199

    R202

    E203

    R215

    E222

    K223�

    A224�

    E225�

    V226�

    R227�

    K228�

    R229�

    R230�

    ALA

    SER

    SER

    LEU

    LYS

    ALA

    • Molecule 11: 40S ribosomal protein S7

    Chain H:

    MET

    SER

    ASP

    P4

    K7

    S10

    �Q11

    T14

    E15

    L16

    Q19

    F24

    N29

    P32

    �E33

    �L34

    �K35

    A36

    �D37

    �L38

    R39

    K44

    R47

    E50

    �V51

    A52

    �G53

    �G54

    V60

    V64

    Y70

    H71

    Q74

    E80

    L81

    E82

    �K83

    Q86

    �D87

    R88

    L93

    I98

    K105�

    S106

    R107

    Q108�

    R114

    S115

    R116

    T117

    L118

    H122

    D123�

    L126

    E127

    D128

    R139

    D158

    V159

    Q160�

    D163�

    N164

    N174

    K175

    L176

    S187�

    GLU

    THR

    HIS

    • Molecule 12: 40S ribosomal protein S8

    Chain I:

    MET

    G2

    R5

    Q20

    F21

    R22

    K23

    K24

    R25

    E28

    �L29

    N35

    T40

    V46

    N52

    L58

    R59

    V72

    R92

    T93

    N94

    T95

    L96

    A99

    S120�

    L121�

    G122

    K123�

    LYS

    LYS

    ASN

    THR

    LYS

    ALA

    GLU

    GLU

    GLU

    THR

    ALA

    THR

    T136�

    S137�

    K138�

    N139�

    T140�

    E141�

    R142�

    K143�

  • Page 18 Full wwPDB EM Validation Report EMD-4327, 6FYX

    W144

    A145�

    A146�

    R147�

    A148

    A149�

    I153

    E154

    D158

    R179

    R196

    A199�

    K200�

    K201�

    • Molecule 13: KLLA0E23673p

    Chain J:

    MET

    P2

    Y8

    S9

    K10

    D25

    L28

    K37

    N38

    I45

    L49

    L60

    E64

    L70

    L93

    K100

    D103

    R107

    R108

    L109

    L118

    V127

    Q131

    I134

    G137

    F146

    R149

    L150

    E151�

    K154

    R160�

    T161

    P169

    G170

    R171

    A179�

    A180�

    G181�

    G182�

    E183�

    GLU

    ALA

    ASP

    GLU

    GLU

    • Molecule 14: KLLA0B08173p

    Chain K:

    M1

    D7

    Q13

    H14

    L15

    L21

    V22

    A23

    D26

    E34

    I35

    D36

    N39

    L40

    K44

    L49

    Y64

    L76

    H85

    �I86

    A89

    L92

    �A93

    �G94

    �Q95

    �S96

    �GLY

    ASP

    GLN

    ARG

    PRO

    GLN

    GLY

    LYS

    LYS

    TYR

    • Molecule 15: KLLA0A10483p

    Chain L:

    MET

    S2

    �T3

    �E4

    �L5

    �T6

    V7

    E10

    R11

    K26

    �A27

    N28

    �R29

    �K30

    K36

    D55

    K56

    R67

    G68

    K69

    R79

    V111

    R116�

    V117

    V125

    T134

    V135

    R136

    S144�

    A145�

    T146�

    G147�

    K148�

    A149�

    N150�

    K151�

    Q152�

    F153�

    A154�

    K155�

    F156�

    • Molecule 16: 40S ribosomal protein S12

    Chain M:

    MET

    SER

    ASP

    VAL

    GLU

    GLU

    VAL

    GLN

    GLN

    VAL

    PRO

    VAL

    ALA

    GLU

    LEU

    THR

    I17

    �E18

    �D19

    �A20

    �L21

    �K22

    V23

    R26

    �T27

    �S28

    L29

    �V30

    �H31

    �D32

    �G33

    �L34

    �A35

    �R36

    �G37

    L38

    R39

    E40

    L45

    �T46

    R47

    �G48

    �E49

    �G50

    �Q51

    �L52

    A53

    �V54

    L55

    V56

    �E57

    �S58

    �V59

    �T60

    E61

    �E62

    �A63

    I64

    L67

    V68

    L71

    �A72

    T73

    �E74

    �ASN

    N76

    �V77

    P78

    L79

    �I80

    �K81

    V82

    A83

    �D84

    �A85

    �K86

    �Q87

    �L88

    G89

    E90

    �W91

    A92

    G93

    �L94

    G95

    �K96

    �I97

    �D98

    �R99

    �D100�

    G101

    N102�

    A103�

    R104

    K105�

    V106�

    A109�

    S110�

    K115�

    N116�

    W117

    G118�

    A119�

    D120�

    T121�

    Q122�

    E123�

    R124

    E125

    I126�

    L127

    L128�

    E129�

    H130�

    F131�

    S132�

    Q133�

    Q134�

    • Molecule 17: KLLA0F18040p

    Chain N:

  • Page 19 Full wwPDB EM Validation Report EMD-4327, 6FYX

    M1

    �G2

    �R3

    K7

    �G8

    K9

    R20

    E83

    L88

    K107

    D110�

    L115

    I116

    R121

    S143�

    A144�

    T145�

    A148�

    L149�

    V150�

    N151�

    • Molecule 18: 40S ribosomal protein S14

    Chain O:

    MET

    ALA

    ASN

    VAL

    VAL

    GLN

    ALA

    LYS

    D9

    �N10

    �S11

    F20

    E37

    A40

    R41

    V42

    K49

    �A50

    D51

    R52

    Q65

    K72

    R84

    A85

    T86

    S91

    K92

    T93

    P94

    G95

    R114

    D117�

    D124

    S125

    T126

    L137

    • Molecule 19: KLLA0F07843p

    Chain P:

    MET

    SER

    GLU

    ALA

    ALA

    ALA

    PRO

    ARG

    LYS

    ARG

    SER

    PHE

    K13

    Y17

    L36

    A37

    R40

    L49

    �S50

    E51

    �K52

    L56

    K59

    L60

    E69

    N70

    �E71

    �K72

    H79

    P87

    E88

    M89

    V94

    V105

    M111

    V126

    R127�

    H128

    G129�

    ARG

    ALA

    GLY

    ALA

    THR

    THR

    SER

    ARG

    PHE

    ILE

    PRO

    LEU

    ARG

    • Molecule 20: 40S ribosomal protein S16

    Chain Q:

    MET

    SER

    T3

    K26

    Q40

    L52

    D58

    V69

    V78

    R82

    Q83

    K87

    G88

    L89

    Q94

    V97

    D113

    L116

    K127

    R137

    F138

    R143

    • Molecule 21: KLLA0B01474p

    Chain R:

    MET

    G2

    R3

    V4

    R5

    R11

    M26

    Q29

    D36

    L46

    T55

    K63

    V66

    R78

    K81

    �D82

    V88

    �S89

    A90

    L91

    D92

    S96

    �N97

    �D98

    V99

    �L100

    N101�

    T106

    L109

    V110

    N111�

    L115�

    K116�

    L117

    V124�

    S125�

    A126�

    V127�

    R128�

    D129�

    R130�

    R131�

    PHE

    ARG

    LYS

    ARG

    ASN

    • Molecule 22: KLLA0B01562p

    Chain S:

    MET

    S2

    �L3

    V4

    �V5

    Q6

    �E7

    �Q8

    �G9

    L15

    R41

    D51

    R57

    L61

    M73

    F85

    L86

    N87

    D91

    V92

    D97

    L105

    E106

    S107

    K108

    L109

    R110

    D111�

    E114

    R120�

    R123�

    F128�

    R134

    G135

    Q136�

    H137

    T141�

    G142�

    R143

    R144�

    R145�

    A146�

    • Molecule 23: KLLA0A07194p

    Chain T:

  • Page 20 Full wwPDB EM Validation Report EMD-4327, 6FYX

    MET

    P2

    D8

    N16

    N17

    Q23

    L28

    Q48

    I65

    R68

    L79

    R86

    G87

    V88

    K110

    L111

    I124

    S125

    D126

    L129

    D133

    L140

    E141�

    D142�

    E143�

    E144�

    • Molecule 24: KLLA0F25542p

    Chain U:

    MET

    SER

    GLN

    VAL

    GLU

    LYS

    LYS

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    GLU

    GLN

    GLN

    Q15

    �E16

    �V17

    �V18

    �I19

    T27

    V31

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    K52

    V56

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    E74

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    • Molecule 25: 40S ribosomal protein S21

    Chain V:

    M1

    L11

    Y12

    S30

    Y50

    E64

    �A65

    D66

    D76

    K80

    R87

    • Molecule 26: 40S ribosomal protein S22

    Chain W:

    MET

    T2

    Q24

    V25

    R28

    P29

    I47

    S58

    C72

    P77

    R78

    D85

    K88

    L104

    V129

    Y130

    • Molecule 27: KLLA0B11231p

    Chain X:

    MET

    G2

    K3

    G4

    K14

    L15

    R16

    L34

    S41

    I59

    Q63

    V72

    N79

    D90

    N94

    F107

    V127

    E138

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    • Molecule 28: 40S ribosomal protein S24

    Chain Y:

    MET

    S2

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    �A4

    R20

    V35

    S36

    L74

    K84

    E98

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    R105

    R111

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    • Molecule 29: KLLA0B06182p

    Chain Z:

  • Page 21 Full wwPDB EM Validation Report EMD-4327, 6FYX

    MET

    PRO

    PRO

    LYS

    GLN

    GLN

    LEU

    SER

    LYS

    ALA

    ALA

    LYS

    ALA

    ALA

    ALA

    ALA

    MET

    ALA

    GLY

    GLY

    LYS

    LYS

    SER

    LYS

    LYS

    LYS

    TRP

    S28

    �K29

    �K30

    �S31

    �H32

    �K33

    �D34

    �K35

    �A36

    �K37

    �H38

    A39

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    �V41

    L42

    �D43

    �Q44

    D45

    �K46

    I50

    �M51

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    �E53

    �A54

    �P55

    T56

    D67

    �R68

    �F69

    L75

    A79

    �L80

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    �H82

    �L83

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    �N85

    �E86

    �G87

    �I88

    I89

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    �Q98

    �A99

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    • Molecule 30: 40S ribosomal protein S26

    Chain a:

    MET

    P2

    K12

    K13

    D33

    R38

    M39

    A48

    Y59

    L64

    I75

    V84

    R89

    R93

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    ALA

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    ALA

    ALA

    LYS

    LYS

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    LEU

    • Molecule 31: 40S ribosomal protein S27

    Chain b:

    MET

    V2

    L3

    E15

    L21

    K22

    T23

    L24

    H31

    P38

    �G39

    T52

    E57

    S60

    �T61

    V62

    E75

    K82

    • Molecule 32: 40S ribosomal protein S28

    Chain c:

    MET

    ASP

    THR

    K4

    �T5

    �P6

    K14

    V15

    L16

    D35

    R38

    P47

    V48

    R49

    E50

    M57

    �E58

    S59

    E60

    R64

    �R65

    �L66

    R67

    • Molecule 33: 40S ribosomal protein S29

    Chain d:

    MET

    A2

    �H3

    E4

    K16

    R19

    L30

    N37

    I38

    D39

    R40

    Q41

    R56

    • Molecule 34: 40S ribosomal protein S30

    Chain e:

    MET

    G2

    �K3

    �V4

    H5

    L8

    A11

    Q17

    Q24

    E25

    K26

    K36

    V47

    T48

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    �N51

    �G52

    �K53

    �R54

    �K55

    S61

    �SER

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    • Molecule 35: Ubiquitin-40S ribosomal protein S27a

    Chain f:

  • Page 22 Full wwPDB EM Validation Report EMD-4327, 6FYX

    MET

    GLN

    ILE

    PHE

    VAL

    LYS

    THR

    LEU

    THR

    GLY

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    • Molecule 36: KLLA0E12277p

    Chain g:

    MET

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    R11

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    • Molecule 37: 60S ribosomal protein L41-A

    Chain h:

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    A3

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    �K8

    R9

    �T10

    R11

    �R12

    �L13

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    �R18

    �K19

    �V20

    �R21

    �A22

    �R23

    �S24

    �K25

    • Molecule 38: Eukaryotic translation initiation factor 1A

    Chain i:

    MET

    G2

    �K3

    �K4

    �N5

    �T6

    �K7

    �G8

    R13

    R14

    G15

    K16

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    ASP

    ILE

    • Molecule 39: Eukaryotic translation initiation factor 2 subunit alpha

    Chain j:

    MET

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    �C6

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    �R55

    �I56

    �R57

    �S58

    K61

    �L62

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    �V65

    �G66

  • Page 23 Full wwPDB EM Validation Report EMD-4327, 6FYX

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    • Molecule 40: Eukaryotic translation initiation factor 2 subunit gamma

    Chain k:

    MET

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  • Page 24 Full wwPDB EM Validation Report EMD-4327, 6FYX

    • Molecule 41: Eukaryotic translation initiation factor 2 subunit beta

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    • Molecule 42: Eukaryotic translation initiation factor 5

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    • Molecule 43: Eukaryotic translation initiation factor 3 subunit A

    Chain o:

  • Page 25 Full wwPDB EM Validation Report EMD-4327, 6FYX

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  • Page 26 Full wwPDB EM Validation Report EMD-4327, 6FYX

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    • Molecule 44: Eukaryotic translation initiation factor 3 subunit B

    Chain p:

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  • Page 27 Full wwPDB EM Validation Report EMD-4327, 6FYX

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    • Molecule 45: eIF3c,eIF3c

    Chain q:

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