Species (sample #1) ：Geotrichum fici

Where： from bread (initially green-grey fungi which grow beside white colony)

How collected and isolated: Transferred from food into plate, #1 growth into white fungi but still have small green colony in the middle of plate (Fig3 up). 2nd subculture gave the colony same white so the green one probably the contamination (Fig6 down)Evidence:

1 2 3

4 5 6 7

Characteristics:White soft fluffy colony with radial-like edge (Fig6 (26d), Fig7(up 35d, down 20d)). Fig1, 2, 4, 5 are permanent slides with conidia forming as unequal-shaped arthrospores developed from segmentation (Fig2&4). Have a pineapple (fruit) smelling at begin and yogurt like smelling after 1 month (G. fici)

From lab book 1: Illustrated Genera of Imperfect Fungi:No conidiomata (Moniliales) conidia no coil (1b) hyaline color (10a) conidia 1 celled (11a)

conidiophores absent (12a) Saprophytic (13b) conidia (arthospores) formed by segmentation of hyphae, rod-shaped (15b) Geotrichum (P68)

Species (sample 3 & 7) ：Basipetospora rubra

Where： #3 Strawberry (white fungi), #7 cooked red bean (yellow fungi)

How collected and isolated: Transferred from food into plate, #7 growth from yellow to white fungi (Fig9). 2nd subculture gave both #3 & 7 the same white colony with same micro-morphology (#3: Fig1-4; #7: Fig5-9)Evidence:

1 2 3 4

5 6 7 8 9

Characteristics:White soft fluffy colony with radial-like edge,(Fig4&9, up 26d, down 16d)

From lab book 1: Illustrated Genera of Imperfect Fungi:No conidiomata (Moniliales) conidia no coil (1b)conidiophore hyaline (Dematiaceae)(10a)1 celled conidia (11a) conidiophores present(12b) No powdery conidial state (19c) Conidia distinct shape from apical cells of conidiophore (20a) Phialides conidiophores (21a) Conidia catenulate (22a) Conidia

exogenous, simple conidiophores (23b) Conidia not blastopores or botryoblastospores (26b) Conidiophores globle arthrospores (28b) Basipetospora (P70)

From Book 2 Soil and Seed Fungi Character: B.rubra is distinguished by up to 13 spores together in chains (P199)

Species (sample 4): Penicillium

Where：#4 from cooked red bean (green molds), #6 from beef soup (white mold)

How collected and isolated: Transferred from food into 1stplate. Following twice subculture gave not similar macro morph of colony (Fig11) but all micro morph were same Evidence:

1 2 3 4 5 6 7

8 9 10 11 12Characteristics:Growth as white surface at first and in 3-5 days turn into green with white (new hyphae) edge. Older one with yellow coloration (Fig11 (up 25d, down 7d), Fig12-27d). Conidia not growth into chains form incompact brush (Gliocladium spp. in compact) Strong Penicillium (old wet moldy wood) smelling. Biverticillate.

From lab book 1: Illustrated Genera of Imperfect Fungi:No conidiomata (Moniliales) conidia no coil (1b) conidiophore hyaline (10a) 1 cell globose conidia (11a) conidiophores distinct, short (12b) Not conidial state of powdery mildew (19c) conidia distinct in shape from apical cells of conidiophore (20a) Conidiophore mostly branched, phialides (21b) Conidia not compact head, conidiophore branched near apex (38b) Conidia in basipetal chains (41a) conidiophores

separate, not in columns or cushion(42a) Conidia phialospores, phialides upright brushlike (43b)

Penicillium (P94)

From Lab book2 A Laboratory Guide of Commen Penicillium Species

Species (sample 6): Penicillium crustosum

Where：Beef soup (white colony)

How collected and isolated: Originally fungi in beef soup was white, probably it was young. 1st transfer (Fig7 up) and later subculture were all successful growth to blue green (Fig7 down)Evidence:

1 2 3 4 5 6 7

8 9 10 11 12 13Characteristics:Yellow back on media side, powder on surface. Conidia growth in chain (Fig6)No smelling, coloration the media to yellow (Fig7 (up 27d, down 7d), Fig13-12/3/2014)

From lab book 1: Illustrated Genera of Imperfect Fungi:No conidiomata (Moniliales) conidia no coil (1b) conidiophore hyaline (10a) 1 cell globose conidia (11a) conidiophores distinct, short (12b) Not conidial state of powdery mildew (19c) conidia distinct in shape

from apical cells of conidiophore (20a) Conidiophore mostly branched, phialides (21b) Conidia not compact head, conidiophore branched near apex (38b) Conidia in basipetal chains (41a) conidiophores separate, not in columns or cushion(42a) Conidia phialospores, phialides upright brushlike (43b)

Penicillium (P94)

From Lab book2 A Laboratory Guide of Commen Penicillium SpeciesSubgenus: Penicillium (no cleistothecia or gymnothecia)biverticillate, phialides nearly equal length to metulae,Distinguish features: Matured on CYA is grey green but not bright yellow green, blue green as young. Young conidia around the margins are often blue green. Will produce readily break conidia (like powder) which easily loose when jarred. Produce yellow to brown soluble pigment. Flat colony.

Species (sample 10.1): Penicillium chrysogenum

Where：Beef soup

How collected and isolated: Beef soup 1st transfer (Fig4), in 2nd culture plate two fungi growth (Fig5) and sub-cultured from middle green large one (Fig6)Evidence:

1 2 3 4 5

6 6 7 8 9 10

Characteristics:Green colony with yellow color in media side; relative smooth dry surface with downy plan colony and was collected on indoor food (P. chrysogenum, many other common indoor spp. are wrinkle radiate-like surface. P. notatum produce liquid on surface. P. verrucosum become yellow after long time growth (sample keep same color)) (Fig8-12/3/2014, Fig9 (up 27d, down 7d), Fig10 (up 7d, down 25d). No smelling

From lab book 1: Illustrated Genera of Imperfect Fungi:No conidiomata (Moniliales) conidia no coil (1b) conidiophore hyaline (10a) 1 cell globose conidia (11a) conidiophores distinct, short (12b) Not conidial state of powdery mildew (19c) conidia distinct in shape from apical cells of conidiophore (20a) Conidiophore mostly branched, phialides (21b) Conidia not compact head, conidiophore branched near apex (38b) Conidia in basipetal chains (41a) conidiophores separate, not in columns or cushion(42a) Conidia phialospores, phialides upright brushlike (43b)

Penicillium (P94)

From Lab book2 A Laboratory Guide of Commen Penicillium Species

Species (sample 8): Aspergillus flavipes

Where：the 3rd time transferred assigned fungi PF3-114 was contaminated, so contamination was collected

How collected and isolated: transferred directed from culture media plat and then subculture (Fig4&5)Evidence:

1 2 3 4 5

6 7 8 9

Characteristics:Yellow colony with golden to sliver powder on mycelium, after mature produce yellow liquid drops (Fig4 (up 60d, down 15d), Fig5 (7d)). Conidia produced as botryoblastospores (Fig 6-9) Edge of the colony typically irregular.

From lab book 1: Illustrated Genera of Imperfect Fungi:Common genera have septate(1b)conidiophore distinguish, conidia 1 celled (3a) conidiophores separate not tight cluster (4d) conidiophore branched (15a) conidia in chains (16a) conidia basipetal (17b)conida (phialides) born from conidiophore at swollen apex (19a)Aspergillus (P94) From Lab book2 A Laboratory Guide of Commen Aspergillus Species and their Teleomorph

1. Predominantly biseriate 2. Colony <5cm 3. Colony yellow-tan 4. Not green when mature on MEA 6. On CYA dominate by golden to orange sclerotia 7. Becoming tan-yellow in age 9. Conidial head radiate

10. Tan to cinnamon all media and colony <3.5cm 11. Conidia abundant, vesicle <25µm 12 conidia on CYA palar A. flavipes (P40)

Species (Sample 10.2): Phialophora bubakii (Anamorph), Chartosphaeria bubakii (Teleomorph)

Where：Beef soup

How collected and isolated: Beef soup 1st transfer (Fig6), in 2nd culture plate (Fig7) two fungi growth and sub-cultured from small grey one (Fig7-9)Evidence:

1 2 3 4

5 6 7 8 9

Characteristics:Dark grey colony (small one located in outside on media in Fig.6 down, Fig7&8- 7d, Fig9 212d); Rough fluffy surface with mountain-like rising in the middle and black coloration in the media side. There is no clear conidia on conidiophore which might because the spores growth one by one and separate when matured. Two kinds conidia (allantoid and ellipsoidal)

From lab book 1: Illustrated Genera of Imperfect Fungi:No conidiomata (Moniliales) conidia no coil (1b) conidiophore dark color (10b) 1 celled conidia (105a)short or absent conidiophore(106a) conidia not blastospores, not budding, (107d) not arthrospores (108b) phialospores (109b) (114d) conidia ovoid to rod-shaped, no setae (115c) conidia without appendage(116c) conidia ovoid, not catenulate (117b) phialides elongate with flaring collar, conidia hyaline(118a) Phialophora (P88)

From lab book 2: More Dematiaceous HyphomycetesP452 Phialophora collarettes not darker than phialides (2) conidia not spherical (4) phialides mostly solitary (5) conidia of two kinds P. bubakii Species (Grass disease): Curvularia lunatus (Anamorph), Cochliobolus lunatus (Teleomorph)

Where：Grass disease

How collected and isolated: Cut grass with the disease infected part, clean with 70% EtOH, 10% bleach, and then 3 times water. Culture leaf on media plate with several times transfer to isolate culture (Fig6,7)

Evidence:

1 2

3 4 5 6 7

Characteristics:Dark grey-green coloration of colony on both surface and under face to the media side (Fig 6 (up 24d, down backside). Relative down flat colony surface (Fig7-7d). Cause leave (grass) turns brown spots and zonate lesions. Spindle-like spores are with typically 4 segments (cells) with darker middle and lighter two ends (lunatus) (Fig 1&2).

From lab book 1: Illustrated Genera of Imperfect Fungi:No conidiomata (Moniliales) conidia no coil (1b) conidiophore dark color (10b) 3 more celled conidia, phragmospores (105c) Conidia exogenous (156b) simple conidiophore (157b) Conidia broader (length not 3-4 times width) (159b)Conidia formed distinct from conidiophore cells(160b) Conidiophore single, seperated(161b) Conidiophore tall (165b)Conidiophore indeterminated, clongating after each successive conidial formation (172b) Conidiophore elongating sympodially (174b) No appendages (176n) Conidia not confined to apex of conidiophore (177b) Straight (litter narrowed end) conidia (181b) Conidia not catenulate, usually 4 celled (183b) Curvularia (P122)Species (Sample 14): Chrysosporium keratinophilum

Where：Soil

How collected and isolated: Victor

Evidence:

1 2 3

4 5

Characteristics:From book Soil and Seeds Fungi: septa hyphae without clamp form conidia spores Deuteromycetesno conidiomata (3)form conidia (4)Chrysosporium keratinophilum (P219)

Species (Sample 15):

Where：Soil

How collected and isolated: VictorEvidence:

1 2 3

4 5

Characteristics:

Species (Sample 17): Penicillium

Where：coke

How collected and isolated: directly transfer from cokeEvidence:

1 2 3

4 5

Characteristics: