g plant extracts and dpph

14
Vallaris glabra (VG) Sampl e Weight of Concen tratio n Abso rnamce values Avera ge sample (gof sample at 517 nm absorbanc Control 0.99 0.99 0.99 0.991 VG1 1.070 1.04 0.35 0.57 0.51 0.49 0.523 1.018 2.07 0.69 0.09 0.08 0.08 0.085 1.037 4.15 1.38 0.06 0.06 0.07 0.063 VG2 1.029 0.05 0.02 0.57 0.58 0.56 0.569 1.073 0.1 0.03 0.22 0.23 0.22 0.223 1.025 0.2 0.07 0.05 0.06 0.06 0.056 VG3 1.033 0.05 0.02 0.57 0.55 0.56 0.562 1.009 0.1 0.03 0.17 0.19 0.18 0.179 1.041 0.2 0.07 0.06 0.06 0.06 0.057 1 2 2 3 3 4 20 40 60 80 100 120

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Page 1: G Plant extracts and DPPH

8/7/2019 G Plant extracts and DPPH

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Vallaris glabra (VG)

Sample Weight of Concentration Absornamce values Average

sample (gof sample at 517 nm absorbanc

Control 0.99 0.99 0.99 0.991

VG1 1.070 1.04 0.35 0.57 0.51 0.49 0.523

1.018 2.07 0.69 0.09 0.08 0.08 0.085

1.037 4.15 1.38 0.06 0.06 0.07 0.063

VG2 1.029 0.05 0.02 0.57 0.58 0.56 0.569

1.073 0.1 0.03 0.22 0.23 0.22 0.223

1.025 0.2 0.07 0.05 0.06 0.06 0.056

VG3 1.033 0.05 0.02 0.57 0.55 0.56 0.562

1.009 0.1 0.03 0.17 0.19 0.18 0.179

1.041 0.2 0.07 0.06 0.06 0.06 0.057

1 2 2 3 3 4

20

40

60

80

100

120

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% Sample Weight of Concentration

Reductionreduction sample (g of sample

Control

0.53 53.7 47.28 VG1 1.070 5.19

0.92 92.3 91.43 1.018 10.37

0.94 94.6 93.64 1.037 20.74

0.43 43.5 42.57 VG2 1.029 0.25

0.78 78.4 77.51 1.073 0.5

0.94 95.3 94.35 1.025 1

0.44 44.2 43.31 VG3 1.033 0.25

0.82 82.8 81.98 1.009 0.5

0.94 95.2 94.25 1.041 1

mL g

50 1.04

0.05 0 1.04 mg

0.1 0 2.07

0.2 0 4.15

mL g

50 1.04

0.05 0 1.04 mg

0.1 0 2.07

0.2 0 4.15

4 5 4 6 8 1

10

20

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40

50

60

70

80

90

100

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Absornamce values Average %

at 517 nm absorban Reductionreduction

0.98 1.02 1.01 1.003

0.07 0.07 0.07 0.068 0.47 47 93.26

0.08 0.08 0.08 0.078 0.93 92.2 92.26

0.09 0.09 0.09 0.088 0.92 91.2 91.2

0.07 0.08 0.07 0.075 0.93 92.5 92.49

0.07 0.08 0.08 0.075 0.93 92.5 92.49

0.05 0.09 0.1 0.081 0.92 91.9 91.96

0.57 0.07 0.07 0.234 0.77 76.7 76.68

0.17 0.08 0.08 0.108 0.9 89.2 89.27

0.09 0.09 0.09 0.091 0.91 90.9 90.93

12 14 16 18 20 22

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mL

50

0.05

0.1

0.2

Sample Weight of Concentration Absornamce values

50% sample ( of sample (mg/ml)

Control 1.01 1.01

VG1 1.041 0.26 0.86 0.85

0.52 0.73 0.72

1.04 0.48 0.45

VG2 1.009 0.25 0.89 0.89

0.5 0.79 0.78

1.01 0.56 0.55

VG3 1.033 0.26 0.88 0.89

0.52 0.79 0.78

1.03 0.56 0.55

g mg

50 1.03

0.05 0 1.03

0.03 0 0.51

0.01 0 0.26

1 1 1 1 1

10

20

30

40

50

60

 

Concentration (mg/ml)

%re

duction

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at 517 nm Average % Sample Weight of Concentra

absorbanceReduction 70% sample ( of sample

1.01 1.009 Control

0.85 0.852 15.55 VG1 1.025 0.26

0.72 0.721 28.57 0.51

0.44 0.455 54.95 1.03

0.89 0.886 12.19 VG2 1.073 0.27

0.78 0.782 22.49 0.54

0.54 0.551 45.38 1.07

0.89 0.886 12.19 VG3 1.029 0.26

0.78 0.781 22.62 0.51

0.55 0.555 44.98 1.03

1

10

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30

40

50

60

70

1

1.041mg

Linear Regression for1.041mg

1.009 mg

Linear Regression for1.009 mg

1.033 mg

Linear Regression for1.033 mg

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tion Absornamce values at 517 nmAverage %

(mg/ml) absorban Reduction

1.01 1.01 1.01 1.010

0.89 0.87 0.87 0.877 13.11

0.76 0.76 0.76 0.760 24.74

0.54 0.53 0.53 0.535 47.03

0.85 0.85 0.85 0.850 15.75

0.7 0.7 0.7 0.699 30.75

0.41 0.4 0.39 0.400 60.34

0.87 0.88 0.87 0.872 13.61

0.77 0.76 0.78 0.767 24.01

0.55 0.55 0.54 0.545 46

1 1 1 1

1.025 mg

LinearRegression for1.025 mg

1.073 mg

LinearRegression for1.073 mg

1.029 mg

LinearRegression for1.029 mg

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Sample Weight of Concentration Absornamce values at 517 nmAverage

50% sample ( of sample (mg/ml) absorban

Control 1.01 1.01 1.01 1.010

VG1 1.041 0.26 0.87 0.87 0.86 0.868

0.52 0.75 0.75 0.76 0.753

1.04 0.52 0.5 0.5 0.507

VG2 1.009 0.25 0.89 0.9 0.9 0.895

0.5 0.81 0.81 0.81 0.809

1.01 0.63 0.62 0.62 0.623

VG3 1.033 0.26 0.91 0.91 0.91 0.909

0.52 0.82 0.82 0.82 0.820

1.03 0.61 0.6 0.6 0.603

1.82 45.98

1 1 1 1 1 1

10

20

30

40

50

601.0

Linfor

1.0Linfor

1.0

Linfor

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2.16 35.7

0.78 39.51

IC50 1.041 g = 1.05

1.009 g = 1.34

1.033 g = 1.25

AEAC (mg AA/100 g)= 369.26

288.82

310.63

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% Sample Weight of Concentration Absornam

Reduction 70% sample ( of sample (mg/ml)

Control 1.01

14 VG1 1.025 0.26 0.89

25.42 0.51 0.78

49.79 1.03 0.54

11.39 VG2 1.073 0.27 0.85

19.84 0.54 0.73

38.3 1.07 0.47

9.97 VG3 1.029 0.26 0.87

18.82 0.51 0.8

40.31 1.03 0.55

g mg

50 1.07

0.05 0 1.07

0.03 0 0.54

0.01 0 0.27

41 g

ear Regression1.041 g

09ear Regression1.009

33

ear Regression1.033

10

20

30

40

50

60

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1.01 43.98

2.87 48.02

1.57 41.65

IC50 1.025 g = 1.11

1.073 g = 0.98

1.029 g = 1.16

AEAC (mg AA/100 g)= 347.36

394.32

332.75

1 1 1 1

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ce values at 517 nmAverage % Sample Weight of Concentra

absorban Reduction 100% sample ( of sample

1.01 1.01 1.011 Control

0.87 0.88 0.881 12.83 VG1 1.037 0.26

0.78 0.79 0.781 22.72 0.52

0.55 0.54 0.542 46.36 1.04

0.85 0.86 0.852 15.69 VG2 1.018 0.25

0.72 0.72 0.721 28.72 0.51

0.46 0.46 0.461 54.37 1.02

0.88 0.88 0.879 13.06 VG3 1.070 0.27

0.79 0.79 0.790 21.83 0.54

0.56 0.56 0.558 44.81 1.07

510

15

20

25

30

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40

45

50

1.025 g

Linear Regressionfor 1.025 g

1.073

Linear Regressionfor 1.073

1.029 g

Linear Regressionfor 1.029 g

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IC50

AEAC (mg

1 1

 

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tion Absornamce values at 517 nmAverage %

(mg/ml) absorban Reduction

1.03 1.02 1.02 1.022

0.91 0.9 0.89 0.902 11.8

0.77 0.77 0.76 0.764 25.27

0.55 0.54 0.54 0.545 46.66

0.92 0.91 0.92 0.918 10.17

0.84 0.84 0.84 0.839 17.97

0.68 0.69 0.68 0.684 33.06

0.92 0.91 0.92 0.918 10.24

0.84 0.84 0.84 0.838 18.03

0.68 0.68 0.67 0.676 33.88

1.037 g

LinearRegression for1.037 g

1.018 g

LinearRegression for1.018 g

1.07 g

LinearRegression for1.07 g

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1.11 44.31

2.62 29.93

2.32 29.48

1.025 g = 1.1

1.073 g = 1.58

1.029 g = 1.62

AA/100 g)= 350.7

244.52

239.25