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Leica StereoZoom®

User Manual

Leica StereoZoom User Manual2

ContentsPage

InformationSafety rules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7Description: Features, lens system . . . . . . . . . . . . . . . . . . .8Models . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10

Design System diagram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12Incident-light equipment . . . . . . . . . . . . . . . . . . . . . . . . . . .14Transmitted-light equipment . . . . . . . . . . . . . . . . . . . . . . . .14Objectives . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16Eyepieces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16High-performance lighting . . . . . . . . . . . . . . . . . . . . . . . . . .17Leica L2 cold-light source . . . . . . . . . . . . . . . . . . . . . . . . . .17Graticules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18Camera assembly for Leica S6 D and S8 APO . . . . . . . . .20

OperatingControls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22Interpupillary distance and exit pupil . . . . . . . . . . . . . . . .23Eyecups . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23Setting working distance and focusing . . . . . . . . . . . . . . .24Zoom magnification changer . . . . . . . . . . . . . . . . . . . . . . .25Diopter adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28Photography with Leica S6 D and S8 APO . . . . . . . . . . . .32

Special notesWhat do I do if . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33Care and maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34Calculating total magnification and field diameter . . . . .37 Optical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38Dimension . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41Leica Microsystems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47

Leica StereoZoom User Manual 3

Dear Customer

Thank you for the trust you have shown in choosing oneof our products. We hope you will have much enjoymentand success with your new stereomicroscope from LeicaMicrosystems.

With its new StereoZoom® line the Swiss Stereomicro-scopy Business Unit offers a comprehensive range ofstereomicroscopes for every application. The Leica L2cold-light illuminator – also new – transforms any of theinstruments into a compact, streamlined complete outfitof modern design. Possible applications range from in-spection and assembly work, OEM and schools to qualityassurance functions. The stereomicroscopes, cold-lightsource and stand are all antistatic to protect againstESD. A Terminator version is available for problematicworking areas in electronics.

One of our major development goals was to make ournew StereoZoom® line simple and intuitive to use. Never-theless, please take the time to read these instructions.They will familiarize you with your stereomicroscope’sadvantages and enable you to use it to best effect. If youhave questions at any time, please contact your Leica representative or Leica Microsystems (Switzerland) Ltd,Heerbrugg, Switzerland. We will be glad to help you. We place great emphasis on customer service – beforeand after purchase.

Read the instructions for use and safety instructions before putting the stereomicroscope into operation.

Leica S4 E, S6 E, S6, S6 T, S6 D and S8 APO stereomicro-scopes are precision optical instruments for making tech-nical and scientific objects, object details or specimensmore easily visible by means of magnification. Stands, illuminators and accessories complete the equip-ment.

• Use of the instrument other than in the manner de-scribed in these instructions could result in personalinjury or material damage.

• Never fit other equipment connectors or dismantle optical systems and mechanical parts unless the usermanual gives express instructions for doing so.

• The Leica S4 E, S6 E, S6, S6 T, S6 D and S8 APO stereo-microscopes are intended primarily for indoor use.

• If used outdoors the stereomicroscope must be pro-tected against dust and moisture. Leica Microsystemselectric illuminators and stands must not be used outdoors.

Repair work must only be carried out by service engineerstrained by Leica Microsystems. Only original spare partsfrom Leica Microsystems are to be used.

• Make sure that personnel using this stereomicroscopehave read and understood these instructions, espe-cially the safety instructions.

• Take steps to ensure that Leica S4 E, S6 E, S6, S6 T, S6 D and S8 APO stereomicroscopes are only operated, serviced and repaired by authorized and trained personnel.

General instructions

Intended application

Improper use

Place of use

Service work

Obligations of theoperator

4 Leica StereoZoom User Manual

Safety rules

5Leica StereoZoom User Manual

Electrically operated equipment

Handle liquids carefully.Liquids spilt on the instrument – can cause the stereomicroscope and other equip-

ment to become electrically live and injure persons,

– can cause damage to the instrument.

Check regularly to ensure that the mains cable is undamaged and avoid jerking or pulling the cable hard.Faulty mains cables • can injure persons,

• can cause the stereomicroscope and other equip-ment to become electrically live and injure persons.

Ensure the cable is not in a position where someonecould accidentally catch it, otherwise the instrumentcould tip over and fall, damaging itself or other equip-ment, or injuring people nearby.

Electrical equipment may only be repaired by authorizedLeica personnel.Disconnect the mains cable before opening the instru-ment. Touching the open instrument when voltage is applied may result in injury.

Make sure that the instrument is set for the correctmains voltage. Incorrect setting can damage the instru-ment.

Liquids

Mains cable

Opening the instrument

Mains voltage

Safety instructions

Only devices with the correct power rating may be connected to the transformers. Overloading can result in damage to the instrument.

• Disconnect the mains cable from the mains supplybefore changing lamps.

• Never put your hand in the lamp housing or trans-mitted-light stand when the instrument is connectedto the mains.

• Wait for filament lamps to cool down before changing. Touching hot filament lamps can result in burns.

Observe all statutory accident prevention and environ-mental protection requirements.

Electrically operated accessories for Leica S4 E, S6 E, S6,S6 T, S6 D and S8 APO stereomicroscopes are manufac-tured using state-of-the-art technology and carry an EC declaration of conformity.

Connections

Changing lamps

Statutory regulations

EC declaration of conformity

6 Leica StereoZoom User Manual – Safety instructions


You will come across these symbols in this manual

Safety warningsThis symbol denotes information that must be read andacted upon without fail. Disregarding safety warnings can put persons at risk!

Disregarding safety warnings can also result in malfunc-tioning or damage to the instrument.

Warning against exposed hot points, e.g. filament lamps.Risk of burns if disregarded!

Important informationThis symbol denotes additional information or explana-tions to assist understanding.

Action� This symbol denotes action to be taken.

Additional information• This symbol denotes additional information or

explanations.

Symbols

The optical system of the Leica StereoZoom® line con-sists of two beam paths converging at 12°. Since thepairs of objectives are very close together, the stereo-microscopes can be constructed very “slim” towards the base. This has a number of advantages: less spacerequired for use on bonders and machines, unimpededworking on the object, plenty of room for tools, free viewof the object field.

The Greenough system provides a low-cost solution forcorrecting aberrations such as chromatism, curvature ofthe field of view and distortion. On the new Leica Stereo-Zoom® line the optimally corrected centre of the objec-tive is used for the image. This results in excellent optical performance with large, flat, distortion-free fieldsof vision and high-contrast images with maximum chromatic correction.

The Leica S4 E, S6 E, S6, S6 D and S8 APO stereomicro-scopes including cold-light source and stand are made ofantistatic material with a surface resistance of 2×1011

ohm/square and a discharge time from 1000V to 100V ofunder 2 seconds.

The Leica S6 T Terminator for problematical work areasand the T incident-light stand are made of antistatic ma-terial with a surface resistance of 102–106 ohm/square and a discharge time from 1000V to zero of under 0.1 seconds.

The Leica StereoZoom® S6 D and S8 APO models areequipped with a built-in video/photo tube that allows forsimple, quick setting up of digital, film and analog videocameras.

Greenough optical system

Protection against ESD

Photography


Description

Features

StereoZoom® Zoom Magnification Viewing Extrasangle

Leica S4 E 4.8:1 6.3×–30× 38° ErgoLens

Leica S6 E 6.3:1 6.3×–40× 38° ErgoLens

Leica S6 6.3:1 6.3×–40× 60° ErgoLens

Leica S6 T 6.3:1 6.3×–40× 38° Terminator ErgoLens

Leica S6 D 6.3:1 6.3×–40× 38° Video/photo tubes Ergono-mic objectives

Leica S8 APO 8:1 10×–80× 38° ApochromaticGreenoughsystemApochromaticzoomApochromatic objectivesVideo/photo tube

9Leica StereoZoom User Manual – Description

StereoZoom® is registered as a trademark in the Principal Register of the US Patent and Trademark Office

World first Leica StereoZoom® S8 APO is the first stereomicroscopeon the market with completely apochromatic correctedGreenough system. Apochromatic optics corrects per-fectly chromatic aberrations, eliminates interfering colorfringes and renders even the finest details ultra sharp.Contrast, brilliance, sharpness, resolution, color fidelityand reproduction accuracy are unsurpassed. The advan-tage of apochromatic correction is best observed in objects that have fine, low-contrast structures such aslarge animal cells, cilia plants or metallic microelectronicstructures.

10 Leica StereoZoom User Manual – Description

StereoZoom®

Leica S4 E

StereoZoom®

Leica S6 E

StereoZoom®

Leica S6

Leica Designby Christophe Apothéloz

11Leica StereoZoom User Manual – Description

StereoZoom®

Leica S6 T

StereoZoom®

Leica S6 D

StereoZoom®

Leica S8 APO


7

5

3

1

6

8

Design1 StereoZoom® Op-

tics carriers

2 Objectives

3 Eyepieces

4 Carriers/Focus arms

Detailed Descriptions seeBrochure M1-188-4en.

5 Focus columns

6 Incident-light bases

7 Transmitted-light bases

8 Stages

9 Stands (Swing arm,

Flex arm) Leica S6 E10 446 294

Leica S610 446 295

10 445 36310 445 367

10 445 387

10 446 394

10 446 339

10 446 340

10 446 341

10 445 616

10 446 395

10 446 22810 446 301 10 446 303

10 447 040

10 315 306

10 446 347

10 446 348

Low Eyepoint EyepiecesEyepieces, fixed Eyepieces, adjustable10×/23 10 446 332 10×/23 10 446 333*16×/16 10 446 354 16×/16 10 446 355*20×/12 10 446 356 20×/12 10 446 357*

* Adjustable eyepieces accept reticles, see price list

10 447 057 10 447 05810 447 159

Leica S4 E10 446 293

For Leica L2 fiberoptic light sourcesee separate system diagram page 14


9

4

2Leica S6 T10 446 296

Leica S6 D10 446 297

10 446 34210 446 34310 446 34410 446 345

13 312 610 10 446 299

10 376 070

13 312 714

High Eyepoint Eyepieces All High Eyepoint Eyepieces include EyeguardsEyepiece, fixed Eyepieces, adjustable

* Adjustable eyepieces accept reticles,10×/23 10 446 326 10×/23 10 446 329* see price list

16×/14B 10 445 301 10 447 03625×/9.5B 10 445 302 Spacing Ring required with eyepiece40×/6B 10 445 303 10 445 301, 10 445 302, 10 445 303

Photo / Video documentationLeica DC cameras

Objectivesfor S4 E, S6 E, S6, S6 T

0.32× 10 446 3160.5× 10 446 3180.63× 10 446 3190.75× 10 446 3201.6× 10 446 3212.0× 10 446 322

Lens 10 446 324shield

Adjustable Lens

0.3×–0.4× 10 446 325

Ergolens

0.6×–0.75× 10 446 3230.7×–1.0× 10 446 317

Objetive for S8 APO0.32× 10 446 334APO 0.63× 10 446 335APO 1.6× 10 446 336APO 2.0× 10 446 337

Leica S8 APO10 446 298

13 312 613

13 312 612

13 312 614

13 312 611

1 Incident-light base with stageplate

2 Transmitted-light base withglass stage plate

3 Focus column with microscopecarrier

4 StereoZoom® optics carrier

5 Optional additional objective

6 Eyepieces, fixed and/or adjustable

The components


Design, Basic equipment

5

4

3

2

1

6

Never loosen the 3 screws on the right-hand side of the focus column.

� Remove stage plate.

� Insert 3 hexagon headscrews through the under-side of the baseplate andscrew tight in the focus column.

� Replace stage plate.

� Remove glass stage plate.

� Undo locking device.

� Place incident-light stand on the transmitted-light base and engage in the connecting screw.

� Close locking device.

� Replace glass stage plate.

� Insert universal light guidein the socket at the back(see user manual for the Leica L2 cold-light source).

Focus column ➔Incident-light base

Transmitted-light base ➔Incident-light base


Assembly

� Insert optics carrier carefully in the microscope carrier.

� Fix optics carrier in the desired position with the clamping screw.

� Screw selected objective tightin counterclockwise direction.

� Screw the lens shield tightly into the thread on the Stereo-Zoom® or onto the additional objective.

� Push eyepiece into the eye-piece tube as far as it will go.

� Check that it sits firmly and snugly.

The following eyepieces are available for StereoZoom®:– 10×/23, 16×/16, 20×/12, fixed and adjustable

with 12mm exit pubil– 10×/23B eyepiece fixed and adjustable, for spectacle

wearers, with 22mm exit pupil– 16×/14B, 25×/9.5B, 40×/6B wide-field eyepieces,

adjustable, with 22mm exit pupil. Spacing ring required for spectacle wearers

Optics carrier ➔Stand

Additional objective(optional) ➔

Optics carrier

Lens shield(optional)

Eyepieces ➔Eyepiece tube


Assembly

Leica StereoZoom User Manual

Leica L2 cold-lightsource ➔ Stand

High-performancelighting

17

You can combine your StereoZoom® with a fixed and anadjustable eyepiece. Two adjustable eyepieces are re-quired for equipment which has a reticle in an eyepiecefor measurement or photography purposes. We recom-mend that you also equip the high-performance LeicaStereoZoom® S8 APO with two adjustable eyepieces.

With its fibre-optic light guidesthe Leica L2 cold-light sourceis the illumination of choice forthe Leica S4 E, S6 E, S6 and S6 T stereomicroscopes. Mat-ching adapters are availablefor connecting the Leica L2cold-light source to variousstereomicroscope stands andfor standalone operation.

Detailed information on the construction and use of theLeica L2 can be found in the relevant user manual.

Please note that the universal light guide on the Leica S8 APO can only be used with the sideways-mountedlamp arm.

For higher requirements, e.g. for photography or in combination with the Leica S8 APO, we offer a diverse,high-performance transmitted light stand and low-voltage lighting. Please ask your Leica advisor about theoptions.

Leica StereoZoom User Manual – Assembly

18

The following graticules and stage micrometers are available for calibration:

Graticule 10mm/0.1mmGraticule 5mm/0.1mmGraticule 5mm/0.05mmGraticule 100 div./0.002"Graticule 100 div./0.001"Graticule 150 div./0.0005"CrosshairStage micrometer 50mm, 0.1/0.01mm divisionsStage micrometer 1", 0.001" divisions

For photography, a 10× format reticle is available.

Two adjustable eyepieces are required for equipmentwhich has a reticle in an eyepiece for measurement orphotography purposes.

The graticules can be inserted into adjustable eyepiecesand eyepieces for spectacle wearers:

� Ascertain with the aid of the stereomicroscope onwhich side the vacuum-metalized scale is located.The scale should be visible on the correct side.

� Pull out the insertfrom the bottom ofthe eyepiece andplace it with theknurled side on thetable.

Graticule ➔Adjustable eyepieces

Assembly


19Leica StereoZoom User Manual – Assembly

� Pick up the graticules at the edge so as not to leavefingerprints and slide intothe holder from the side.

� Put the insert back into the eyepiece and press firmly inplace.

� Insert the eyepiece into the eyepiece tube and line upthe graticule by turning theeyepiece in the eyepiecetube.

The measuring process is described in the “Measuring”instructions.

20

LeicaMPS30 / MPS60Photoautomats

11 541 0380,55× – 1,1×

11 543 703 11 543 706

10 445 932

10 446 1208×10 445 30410×10 445 30516×

10 404 207

10 445 541

10 162 226

AdapterSLR

SLRcamera housings

10 445 9291/2”0,5×DC100

10 445 9281/3”0,32×

11 541 0061”, 1×DC150

11 541 0072/3”, 0,63×

11 541 0161/2”, 0,5×

1”, 2/3”, 1/2”, 1/3”C-Mount, CCD cameras

Leica DC cameras

1”C-Mount

CCD camera

3-Chip 1/2”B-Mount

CCD camera

3-Chip 2/3”B-Mount

CCD camera

11 543 702

10 445 9301,0×

10 446 175

10 446 2612/3", 0,63×DC300DC300 FDC350 FDC500

Leica S6 D10 446 297

Leica S8 APO10 446 298

Assembly


21

Leica StereoZoom® S6 D and S8 APO are equipped with abuilt-in video/photo tube that allows for simple, quick set-ting up of digital, film and analog video cameras. Pleaseask your Leica advisor about the options.

You can find detailed information about Leica camerasystems, accessories and image management softwarein the corresponding manuals.

� Remove the dust cover from the video/photo output.� Always close the video/photo output with the dust

cover if there is no camera attached.

� Attach appropriate video or photo objective, accor-ding to which camera is used (see scheme p.20).

The format reticle is used to test the sharpness and picture detail. Borderlines are displayed on the reticle for all film formats.

The left observation beam path and the photo output are aligned with one another. Always judge, therefore,the picture detail and the sharpness in the left eyepiece.

� Insert format reticle into an adjustable eyepiece (see p.18).

� Insert eyepiece with reticle into the left tube.

Camera assembly for Leica S6 D and

S8 APO

Format reticle

Leica StereoZoom User Manual – Assembly

22

1 Magnification changer,right-hand pinion knob with magnification scale

2 S6 models: zoom limiter stop

3 Focusing drive

4 Fixing screwfixes the optics carrier in the microscope carrier

5 Adjustable eyepiece tubes:Interpupillary distance adjustable from 55–75mm

6 Eyepieces

7 Lamp bracket mounting thread (on both sides and at rear)

8 Socket for connecting for the Leica L2 universal light guide (not for Leica S8 APO)

9 Thread for additional objective/lens shield

Overview

Standard controlsand their functions


OperatingOperating

65

24

1

9 7 3

7

8

23

The interpupillary distance can beadjusted from 55–75mm. The exit pupil is the distance between the eye and eyepiece. It is – 12mm on the 10×/23,

16×/16 and 20×/12 standard eyepieces

– 22mm on the 10×/23B, fixed and adjustable, for spectaclewearers, and on the16×/14B, 25×/9.5B and 40×/6Bwide-field eyepieces for spectacle wearers

� Carefully put your eyes againstthe eyepieces and push the eyepiece tubes together orapart until with both eyes youcan see a single shadow-free circular field.

The 10×/23B, 16×/14B, 25×/9.5Band 40×/6B eyepieces for spec-tacle wearers are supplied withdetachable eyecups.

If you do not wear spectacles andwant the eyecups to fit snuglyround your eyes:� Attach the eyecups to the

eyepieces.

You wear spectacles for your work.� Fold the eyecups downwards. • The folded-down eyecups will

protect your spectacle lensesagainst scratching.

Interpupillary distance and

exit pupil

Eyecups

5575

12 / 22

Leica StereoZoom User Manual – Operating

24

To focus the stereomicroscope, raise or lower it using thefocusing drive until the desired object segment is in focus, i.e. inside the objective’s working distance. Theworking distances of the various objectives are listed inthe tables on pages 38–40.

You can operate the focusing drive with either your rightor left hand.

� Position the object under the objective.

� Select the minimum magnification.

• You should select the mini-mum magnification becauseit is easier to find the desired object segment in a large field of vision.

� Look into the eyepieces.

� Focus the object by turning the pinion knob.

Is the focusing drive too stiff/too loose or does the set-upslip down unassisted? You can tension the focusing driveto suit the weight of the set-up and your own needs:

� Take one pinion knob in each hand and turn in opposing directions until the desired tension is reached.

Setting working distance and

focusing

Tension adjustment


Operating

25Leica StereoZoom User Manual – Operating

The optics carrier can be tilted sideways if the user wants to work from a lateral position:

� Loosen the clamping bolt.

� Tilt the optic carrier side-ways to the desired position.

� Tighten the clamping bolt.

All StereoZoom® models are equipped with a continuouslyadjustable magnification changer that can be operatedwith either the right or left hand. The right-hand pinionknob carries a magnification scale. The ranges are:– 0.63 to 3 on the S4 E and– 0.63 to 4 on the S6 models– for S8 APO from 1 to 8

The tables on pages 38 to 40 list the magnifications andfield diameters as a function of magnification changerposition and the eyepiece/objective combination beingused.

� Look into the eyepieces.

� Bring the object into focus

(p.24)

� Rotate the magnification changer until the desired magnification is achieved.

Tilting the opticscarrier to a lateral

position

Zoom magnificationchanger

Changing magnification


OperatingOn the S6 models and for the S8 APO it ispossible to fix the maximum and minimumzoom. The same function can be used to seta fixed level of magnification.

1. Set the minimum zoom level at 1 using thestop on the left-hand pinion knob:

� Loosen the hexagon headscrews on the left-hand pinion knob using the Allenkey supplied.

� Turn the right-hand pinionknob to 1.

� Move the stop on the left-hand pinion knob forwardsuntil it touches the built-inzoom stop.

� Tighten the hexagon headscrews.

Setting thezoom limiter

Example: Setting a zoom

range of 1 to 3.2

1


2. Set the maximum zoom level at 3.2 using the stop onthe right-hand pinion knob:

� Loosen the hexagon headscrews on the right-hand pinion knob.

� Turn the right-hand pinion knobto 3.2.

� Move the stop on the right-hand pinion knob backwardsuntil it touches the built-inzoom stop.

� Tighten the hexagon headscrews.

3.2

28

OperatingDiopter adjustment

and setting parfocality

Preparations

Bring the test object into focus

If you adjust the diopters at the adjustable eyepiece asdescribed below, the focus will remain constant, or par-focal, from minimum to maximum magnification. In otherwords, you will not have to refocus when you changemagnification, but only when you want to examine a higher- or lower-positioned object segment. Make fulluse of this feature, which is not available on every stereomicroscope.• Diopter adjustment is possible within a range

from +5 to –5.Every user has to make the following adjustments only once. If you are using a graticule, the diopter adjustment andparfocality setting procedure is slightly different to thatdescribed here. Please refer to the section on measure-ment in the graticule user manuals.

Setting up diopter with an adjustable and a fixed eyepiece

� Set up illuminator.

� Set interpupillary distance (p.23).

� Set approximate working distance using the focusingdrive (see p.38–40 for the working distances of the various objectives).

� Position a flat test object beneath the objective.

� Set the microscope to minimum magnification.

� Close the eye that is looking into the adjustableeyepiece and look into the fixed eyepiece with the other eye.

� View the test object andbring into focus with the focusing drive.



� Without looking into the eye-pieces, turn the eyelens of theadjustable eyepiece as far aspossible in the “+” direction.

� Close the eye that is looking into the fixed eyepiece andlook into the adjustable eyepiece with the other eye.

� View the test object and slowly turn the eyelens clock-wise (the “–” direction) until the object is in focus.

� Set the microscope to maximum magnification.

� View the test object with botheyes and bring it into sharp focus with the focusing drive.

View the object while zoomingfrom minimum to maximum magni-fication.

• The object should remain inconstant focus (parfocal) at alltimes. If it does not, please repeat the procedure above.

Diopter adjustmentat the adjustable

eyepiece

Checking parfocality


Special notesSetting up diopter with two adjustable eyepieces

� For Leica S6 D and S8 APO, set pinion knob to Vis position.

� Set approximate working distance by means of focusing drive (working distances of various objectives see p.38–40).

� Set up lighting

� Set viewing distance (p.23).

� Set ‘0’ diopters on both eyepieces.

� Place flat test object underthe objective.

� Set lowest magnification.

� View test object through the eyepiece and focus using the focusing drive.

� Set highest magnification.

� Optimize sharpness using the focusing drive.

Preparations

Focusing on test object

31Leica StereoZoom User Manual – Special notes

� Set lowest magnification.

� Do not look into the eyepiece!

� Turn lens counterclockwise inthe ‘+’ direction until it stops.

� Now look into the eyepiece.

� Close one eye.

� With the other eye, observe the test object and turn thelens slowly clockwise in the ‘–’direction, until this eye seesthe object clearly.

� Set the diopter for the othereye in the same way.

� Select highest magnification.

� View object and, if necessary, gently refocus.

� Adjust magnification changerfrom the lowest to the highestmagnification.

• When doing this, the sharp-ness must remain constantover the entire zoom range(parfocal). Otherwise, please repeat the procedure.

Regulating diopters

Testingparfocality

31

32

It is possible to switch between the observation and photo beam paths. Light distribution:

– in ‘Vis’ position: 100% light inboth eyepieces/no light in the video/photo beam path

– in ‘Doc’ position: 100% lightin the right eyepiece/no lightin the left eyepiece/100%light in the video/photo beampath

� When the picture detail and sharpness are set to your satisfaction, switch to the ‘Doc’ position and take your photo.


DOC

VIS

OperatingPhotography with

Leica S6 D andS8 APO

… the field of vision is in shadow?

• Adjust interpupillary distance and ensure that it is correct (p.23).

• Check pupil position (p.23).

… the image will not stay in focus?

• Insert eyepieces correctly (p.16).• Correct diopter adjustment exactly as described

(p.28–31).

… the focusing drive slides down or is difficult to turn?

• Adjust tension (p.24).

If you encounter problems with electrically poweredequipment, first check:• that the voltage selector is set to the correct voltage.• that the mains power switch is in the ‘on’ position.• that the mains power cable is correctly connected.• that all connector cables are correctly connected.• that none of the fuses have blown.

… the image is too dark?

• Set regulating knob high enough.

Photographs are blurred.

• Focus precisely (p.24).• Focus on reticles and carry out diopters-correction

exactly according to the instructions (p.30).• Insert eyepieces all the way in (p.16).• Check that the reticles are firmly placed in the

eyepiece (p.18).

No image on the film

• Switch light divider on the photo tube to the ‘Doc’ position (p.32).

Special notesWhat do I do if …

Remedy

Remedy

Remedy

Remedy

Remedy

Remedy


34

In this section we would like to explain how you can take care of your microscope and give you a few tips oncleaning.

• against moisture, vapours, acids, alkalis and causticsubstances.

Never keep chemicals close to your microscope.

• against improper handling.

Never fit other equipment connectors or dismantle optical systems and mechanical parts unless the usermanual gives express instructions for doing so.

• against oil and grease.

Never grease guides and mechanical parts.

• Cover your microscope with its dust cover during breaks.

• Put dust covers over tube openings, eyepieces, and eyepiece tubes without eyepieces.

• Remove dust with a rubber blower and soft brush.

• Clean eyepieces and objectives with special opticalcleaning cloths and pure alcohol.

• Keep accessories in a dust-free environment whennot in use.

Protect your microscope

Dust and dirt impair your results.

Therefore:

Care


Care

35

Various components are made of plastic or are plastic-coated to make them pleasant to touch and handle. However, incorrect cleaning with unsuitable detergentscan damage the plastic. Please observe the following do’s and don’ts:

• in an ultrasonic cleaner. The plastic may become brittle and eventually break.

• with caustic or acetone-containing substances suchas ether substitute.

• with any other solvents except ethanol and isopropanol.

• warm soapy water, rinsing the plastic afterwards with distilled water.

• ethanol (industrial alcohol) and isopropanol.

Always take appropriate safety precautions when usingethanol and isopropanol.

Cleaning plastic parts

Never clean plastics

To clean plastics safely use

Leica StereoZoom User Manual – Care


You are working with a high-performance precision instrument, whose quality we guarantee.

The warranty covers manufacturing and materials faults,but not damage resulting from negligence or incorrecthandling.

If you treat your valuable optical instrument with due care, it will repay you with the decades of reliable precision forwhich Leica instruments are renowned.

However, should you encounter problems with your micro-scope at any time, please contact your local Leica represen-tative or Leica Microsystems Ltd, Heerbrugg, Switzerland.

We guarantee quality

Special notes

3737Leica StereoZoom User Manual – Special notes

MO Objective magnificationME Eyepiece magnificationz Magnification changer settingNFOV Eyepiece field number. Field numbers are printed

on the eyepieces:10×/23, 16×/16, 20×/12, 10×/23B, 16×/14B, 25×/9.5B, 40×/6B

Example:

MO 1.6× objective

ME 20×/12 eyepiece

z Zoom position 4.0

Magnification in the binocular tube:

MTOT VIS = MO × ME × z 1.6 × 20 × 4 = 128

Field diameter in the object:

NFOV 12 ∅OF = __________ __________ = 1.9mm

MO × z 1.6 × 4

Calculating totalmagnification and

field diameter

Optical Data, Leica S4 E, S6 E, S6, S6 T, S6 D


with additional objectives

0.32× 0.5× 0.63× 0.75× 1.6×

110mm 300mm 200mm 155mm 130mm 55mmTo

tal m

agni

ficat

ion

Working distance

Eyepieces Zoom

pos

ition

*4.0

: S6

40×/6B

10 445 303

6.38.0

10.012.516.020.025.032.040.0

10.112.816.020.025.632.040.051.264.0

12.616.020.025.032.040.050.064.080.0

10.112.816.020.025.632.040.051.264.0

15.820.025.031.340.050.062.580.0

100.0

25.232.040.050.064.080.0

100.0128.0160.0

36.528.823.018.414.411.59.27.25.8

25.320.016.012.810.08.06.45.04.0

19.015.012.09.67.56.04.83.83.0

22.217.514.011.28.87.05.64.43.5

15.011.99.57.65.94.83.83.02.4

9.57.56.04.83.83.02.41.91.5

2.02.63.24.05.16.48.0

10.212.8

3.24.15.16.48.2

10.212.816.420.5

4.05.16.48.0

10.212.816.020.525.6

3.24.15.16.48.2

10.212.816.420.5

5.06.48.0

10.012.816.020.025.632.0

8.110.212.816.020.525.632.041.051.2

115.088.571.957.545.135.928.822.518.0

80.062.450.240.031.225.120.015.612.5

60.047.137.530.023.518.815.011.79.4

70.054.643.935.027.322.017.513.710.9

47.537.129.723.818.614.811.99.37.4

29.623.518.815.011.79.47.55.94.7

3.24.05.06.38.0

10.012.516.020.0

5.06.48.0

10.012.816.020.025.632.0

6.38.0

10.012.516.020.025.032.040.0

5.06.48.0

10.012.816.020.025.632.0

7.910.012.515.620.025.031.340.050.0

12.616.020.025.032.040.050.064.080.0

71.957.546.036.528.823.018.414.411.5

51.240.032.025.620.016.012.810.08.0

38.130.024.019.215.012.09.67.56.0

44.835.028.022.417.514.011.28.87.0

30.123.819.015.211.99.57.65.94.8

19.015.012.09.67.56.04.83.83.0

4.05.06.37.9

10.112.615.820.225.2

6.48.1

10.112.616.120.225.232.340.3

7.910.112.615.820.225.231.540.350.4

6.48.1

10.112.616.120.225.232.340.3

9.912.615.819.725.231.539.450.463.0

15.920.225.231.540.350.463.080.6

100.8

57.546.036.529.122.818.314.611.49.1

40.031.625.320.315.912.710.27.96.4

30.423.819.015.211.99.57.66.04.8

35.027.722.217.813.911.18.96.95.6

24.018.815.012.19.47.56.04.73.8

15.111.99.57.66.04.83.83.02.4

4.76.07.59.4

12.015.018.824.030.0

7.69.6

12.015.019.224.030.038.448.0

9.512.015.018.824.030.037.548.060.0

7.69.6

12.015.019.224.030.038.448.0

11.815.018.823.430.037.546.960.075.0

18.924.030.037.548.060.075.096.0

120.0

48.938.330.724.519.215.312.29.67.7

33.726.721.317.113.310.78.56.75.3

25.320.016.012.810.08.06.45.04.0

29.523.318.714.911.79.37.55.84.7

20.115.812.610.17.96.35.14.03.2

12.710.08.06.45.04.03.22.52.0

10.112.816.020.025.632.040.051.264.0

16.120.525.632.041.051.264.081.9

102.4

20.225.632.040.051.264.080.0

102.4128.0

16.120.525.632.041.051.264.081.9

102.4

25.232.040.050.064.080.0

100.0128.0160.0

40.351.264.080.0

102.4128.0160.0204.8256.0

22.818.014.411.59.07.25.84.53.6

15.912.510.08.06.25.04.03.12.5

11.99.47.56.04.73.83.02.31.9

13.910.98.87.05.54.43.52.72.2

9.47.45.94.83.73.02.41.91.5

6.04.73.83.02.31.91.51.20.9

Fiel

d di

amet

er m

m

Tota

l mag

nific

atio

n

Fiel

d di

amet

er m

m

Tota

l mag

nific

atio

n

Fiel

d di

amet

er m

m

Tota

l mag

nific

atio

n

Fiel

d di

amet

er m

m

Tota

l mag

nific

atio

n

Fiel

d di

amet

er m

m

Tota

l mag

nific

atio

n

Fiel

d di

amet

er m

m

0.630.81.01.251.62.02.53.24.0*

0.630.81.01.251.62.02.53.24.0*

0.630.81.01.251.62.02.53.24.0*

0.630.81.01.251.62.02.53.24.0*

0.630.81.01.251.62.02.53.24.0*

0.630.81.01.251.62.02.53.24.0*

10 446 33210 446 333

10×/2310×/23B

10 446 32610 446 329

16×/16

10 446 35410 446 355

20×/12

10 446 35610 446 357

16×/14B

10 445 301

25×/9.5B

10 445 302

"

39Leica StereoZoom User Manual 39

12.616.020.025.032.040.050.064.080.0

20.225.632.040.051.264.080.0

102.4128.0

25.232.040.050.064.080.0

100.0128.0160.0

20.225.632.040.051.264.080.0

102.4128.0

31.540.050.062.580.0

100.0125.0160.0200.0

50.464.080.0

100.0128.0160.0200.0256.0320.0

18.314.411.59.27.25.84.63.62.9

12.710.08.06.45.04.03.22.52.0

9.57.56.04.83.83.02.41.91.5

11.18.87.05.64.43.52.82.21.8

7.55.94.83.83.02.41.91.51.2

4.83.83.02.41.91.51.20.90.8

2.53.13.94.96.27.89.8

12.515.6

3.95.06.27.8

10.012.515.620.025.0

4.96.27.89.8

12.515.619.525.031.2

3.95.06.27.8

10.012.515.620.025.0

6.17.89.8

12.215.619.524.431.239.0

9.812.515.619.525.031.239.049.962.4

92.074.259.046.937.129.523.518.414.7

65.651.241.332.825.620.516.412.810.2

49.038.730.824.519.215.412.39.67.7

57.444.836.128.722.417.914.411.29.0

38.930.424.219.515.212.29.77.66.1

24.519.215.412.39.67.76.24.83.8

1.82.22.83.54.55.67.09.0

11.2

2.83.64.55.67.29.0

11.214.317.9

3.54.55.67.09.0

11.214.017.922.4

2.83.64.55.67.29.0

11.214.317.9

4.45.67.08.8

11.214.017.522.428.0

7.19.0

11.214.017.922.428.035.844.8

127.8104.582.165.751.141.132.925.620.5

91.471.156.945.735.628.422.917.914.3

68.653.342.934.326.721.417.113.410.7

80.062.249.840.031.124.920.015.712.5

54.042.433.927.021.217.013.610.68.5

33.826.721.417.113.410.78.66.75.4

4.76.07.59.4

12.015.018.824.030.0

7.69.6

12.015.019.224.030.038.448.0

9.512.015.018.824.030.037.548.060.0

7.69.6

12.015.019.224.030.038.448.0

11.815.018.823.430.037.546.960.075.0

18.924.030.037.548.060.075.096.0

120.0

48.938.330.724.519.215.312.29.67.7

33.726.721.317.113.310.78.56.75.3

25.320.016.012.810.08.06.45.04.0

29.523.318.714.911.79.37.55.84.7

20.115.812.610.17.96.35.14.03.2

12.710.08.06.45.04.03.22.52.0

3.54.55.67.09.0

11.214.017.922.4

5.67.29.0

11.214.317.922.428.735.8

7.19.0

11.214.017.922.428.035.844.8

5.67.29.0

11.214.317.922.428.735.8

8.811.214.017.522.428.035.044.856.0

14.117.922.428.035.844.856.071.789.6

65.751.141.132.925.620.516.412.810.3

45.735.628.422.917.914.311.48.97.2

33.826.721.417.113.410.78.66.75.4

40.031.124.920.015.712.510.07.86.3

27.021.217.013.610.68.56.85.34.2

17.013.410.78.66.75.44.33.32.7

6.27.99.9

12.415.819.824.831.739.6

10.012.715.819.825.331.739.650.763.4

12.515.819.824.831.739.649.563.479.2

10.012.715.819.825.331.739.650.763.4

15.619.824.830.939.649.561.979.299.0

24.931.739.649.563.479.299.0

126.7158.4

37.129.123.218.514.611.69.37.35.8

25.620.216.212.910.18.16.55.04.0

19.215.212.19.77.66.14.83.83.0

22.417.614.211.38.97.15.74.43.5

15.212.09.67.76.04.83.83.02.4

9.67.66.14.83.83.02.41.91.5

4.55.77.18.9

11.414.217.822.728.4

7.29.1

11.414.218.222.728.436.445.4

8.911.414.217.822.728.435.545.456.8

7.29.1

11.414.218.222.728.436.445.4

11.214.217.822.228.435.544.456.871.0

17.922.728.435.545.456.871.090.9

113.6

51.140.432.425.820.216.212.910.18.1

35.628.122.518.014.111.39.07.05.6

27.021.116.913.510.68.56.85.34.2

31.124.619.615.812.39.97.96.24.9

21.216.713.310.78.46.75.34.23.3

13.410.68.56.85.34.23.42.62.1

Tota

l mag

nific

atio

n

Fiel

d di

amet

er m

m

Tota

l mag

nific

atio

n

Fiel

d di

amet

er m

m

Tota

l mag

nific

atio

n

Fiel

d di

amet

er m

m

Tota

l mag

nific

atio

n

Fiel

d di

amet

er m

m

Tota

l mag

nific

atio

n

Fiel

d di

amet

er m

m

Tota

l mag

nific

atio

n

Fiel

d di

amet

er m

m

Tota

l mag

nific

atio

n

Fiel

d di

amet

er m

m

35mm 200mm 350mm 77mm 137mm 48mm 98mm

with additional objectives

2.0× 0.3×–0.4× 0.3×– 0.4× 0.6×– 0.75× 0.6×– 0.75× 0.7×–1.0× 0.7×–1.0×

40

75mm 101mm 37mm 25mm 200mm

Apochromats Achromate

0.63× 1.6× 2.0× 0.32×

Tota

l mag

nific

atio

n

Working distance

Eyepieces Zoom

pos

ition

10.012.516.020.025.032.040.050.063.080.0

16.020.025.632.040.051.264.080.0

100.8128.0

25.031.340.050.062.580.0

100.0125.0157.5200.0

40.050.064.080.0

100.0128.0160.0200.0252.0320.0

23.018.414.411.59.27.25.84.63.72.9

16.012.810.08.06.45.04.03.22.52.0

9.57.65.94.83.83.02.41.91.51.2

6.04.83.83.02.41.91.51.21.00.8

6.37.9

10.112.615.820.225.231.539.750.4

10.112.616.120.225.232.340.350.463.580.6

15.819.725.231.539.450.463.078.899.2

126.0

25.231.540.350.463.080.6

100.8126.0158.8201.6

36.529.122.818.314.611.49.17.35.84.6

25.320.315.912.710.27.96.45.14.03.2

15.012.19.47.56.04.73.83.02.41.9

9.57.66.04.83.83.02.41.91.51.2

16.020.025.632.040.051.264.080.0

100.8128.0

25.632.041.051.264.081.9

102.4128.0161.3204.8

40.050.064.080.0

100.0128.0160.0200.0252.0320.0

64.080.0

102.4128.0160.0204.8256.0320.0403.2512.0

20.025.032.040.050.064.080.0

100.0126.0160.0

32.040.051.264.080.0

102.4128.0160.0201.6256.0

50.062.580.0

100.0125.0160.0200.0250.0315.0400.0

80.0100.0128.0160.0200.0256.0320.0400.0504.0640.0

11.59.27.25.84.63.62.92.31.81.4

8.06.45.04.03.22.52.01.61.31.0

4.83.83.02.41.91.51.21.00.80.6

3.02.41.91.51.20.90.80.60.50.4

3.24.05.16.48.0

10.212.816.020.225.6

5.16.48.2

10.212.816.420.525.632.341.0

8.010.012.816.020.025.632.040.050.464.0

12.816.020.525.632.041.051.264.080.6

102.4

71.957.545.135.928.822.518.014.411.49.0

43.935.027.322.017.513.710.98.86.95.5

29.723.818.614.811.99.37.45.94.73.7

18.815.011.79.47.55.94.73.83.02.3

Fiel

d di

amet

er m

m

Tota

l mag

nific

atio

n

Fiel

d di

amet

er m

m

Tota

l mag

nific

atio

n

Fiel

d di

amet

er m

m

Tota

l mag

nific

atio

n

Fiel

d di

amet

er m

m

Tota

l mag

nific

atio

n

Fiel

d di

amet

er m

m

1.01.251.62.02.53.24.05.06.38.0

1.01.251.62.02.53.24.05.06.38.0

1.01.251.62.02.53.24.05.06.38.0

1.01.251.62.02.53.24.05.06.38.0

10 446 33210 446 333

10×/2310×/23B

10 446 32610 446 329

16×/16

10 446 35410 446 355

25×/9.5B

10 445 302

40×/6B

10 445 303

14.411.59.07.25.84.53.62.92.31.8

10.08.06.25.04.03.12.52.01.61.3

5.94.83.73.02.41.91.51.20.90.7

3.83.02.31.91.51.20.90.80.60.5

Optical Data, StereoZoom® S8 APO

Leica StereoZoom User ManualLeica StereoZoom User Manual


with incident-light stand

Dimensions, Leica S4 E, S6 E, S6 T

161

107 23

25

8111

0

351

38°

22

134 22

3

278

393

ø58

ø76

46

278

393

205

144

104

113

148205

Leica S4 E, S6 E, S6 T

Dimensions in mm

42

161

107

23

25

8111

0

351

38°

2265

134 22

3

278

393

ø58

ø76

46

with transmitted-light stand

Dimensions, Leica S4 E, S6 E, S6 T

144

104

113

148205

220

278

393

205

Leica S4 E, S6 E, S6 T

Dimensions in mm


207

107 23

25

8111

0

351

38

22

134 22

3

278

393

ø58

ø76

171

278

393

205

144

104

113

148205

43

with inclining focusing drive

Dimensions, Leica S6


Dimensions, Leica S6 D

120

148

25

8111

0

390

60°

ø15.

8 25

26 35

ø58

ø76

46

144

104

150.4

Leica S6

Dimensions in mm

Dimensionsin mm

Leica S6 D



207

107

23

25

8111

0

351

38

2265

134 22

3

278

393

ø58

ø76

171

278

393

205

144

104

113

148205

220

Leica S6 D


Dimensions, Leica S6 D

Dimensions in mm

Leica StereoZoom User Manual 45Leica StereoZoom User Manual


Dimensions, Leica S8 APO

207

107 23

25

116

75

351

38

22

134 22

3278

393

ø58ø76

171

278

393

205

144

104

113

148205

Dimensions in mm

Leica S8 APO

46

with transmitted-light stand

Dimensions, Leica S8 APO

207

107

23

25

116

75

351

38

2265

134 22

3

278

393

ø58ø76

171

144

104

113

148205

220

278

393

205

Dimensions in mm

Leica S8 APO



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Leica Microsystems develops high-utility end-to-end solutions and innovative cutting-edge technologies ofinternationally renowned quality. The same high qualitystandards are in force in each of the Group’s 11 pro-duction sites in seven countries, making the name Leica synonymous with quality.

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Cédex Tel. +33 1 4732 8585 Fax +33 1 4732 8586Germany: Bensheim Tel. +49 6251 1360 Fax +49 6251 136 155Italy: Milan Tel. +39 02 57 486 1 Fax +39 02 5740 3273Japan: Tokyo Tel. +81 3 543 596 09 Fax +81 3 543 596 15Korea: Seoul Tel. +82 2 514 6543 Fax +82 2 514 6548Netherlands: Rijswijk Tel. +31 70 41 32 130 Fax +31 70 41 32 109Portugal: Lisbon Tel. +35 1 213 889 112 Fax +35 1 213 854 668Singapore: Tel. +65 6 77 97 823 Fax +65 6 77 30 628Spain: Barcelona Tel. +34 93 494 9530 Fax +34 93 494 9532Sweden: Sollentuna Tel. +46 8 625 45 45 Fax +46 8 625 45 10Switzerland: Glattbrugg Tel. +41 1 809 34 34 Fax +41 1 809 34 44United Kingdom: Milton Keynes Tel. +44 1908 666 663 Fax +44 1908 609 992USA: Bannockburn/Illinois Tel. +1 800 248 0123 Fax +1 847 405 0164

and representatives of Leica Microsystems in more than 100 countries.

Leica Microsystems’ Mission is to be the world’s first-choice provider of innovative solutions to our customers’ needs for vision, measurement, lithography and analysis of microstructures.

Leica, the leading brand for microscopes and scientific instruments, has developed from five brand names, all with a long tradition: Wild, Leitz, Reichert, Jung and Cambridge Instruments. Leica symbolizes not only tradition, but also innovation.

The companies of the Leica MicrosystemsGroup operate internationally in five businesssegments, where we rank with the market leaders.

MicroscopyOur expertise in microscopy is the basis for allour solutions for visualization, measurementand analysis of microstructures in life sciencesand industry.

Specimen PreparationWe specialize in supplying complete solutionsfor histology and cytopathology.

Imaging SystemsWith confocal laser technology and image analysis systems, we provide three-dimensionalviewing facilities and offer new solutions forcytogenetics, pathology and material sciences.

Medical EquipmentInnovative technologies in our surgical micro-scopes offer new therapeutic approaches inmicrosurgery. With automated instruments forophthalmology, we enable new diagnostic methods to be applied.

Semiconductor Equipment Our automated, leading-edge measurementand inspection systems and our E-beam lithography systems make us the first choicesupplier for semiconductor manufacturers allover the world.

The Business Units in Leica Microsystems hold the management system certificates for the international standards ISO 9001 and ISO 14001 relating to quality management,quality assurance and environmental management. Ill

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Telephone +41 71 726 33 33Fax +41 71 726 33 99www.leica-microsystems.comwww.stereomicroscopy.com

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