gapdha suppresses heat shock induced dna fragmentation in arabidopsis plant
DESCRIPTION
GAPDHa Suppresses Heat Shock Induced DNA Fragmentation in Arabidopsis plant. 44 C 2h+22 C 24h. No treated. GAPa:GFP. BIP:GFP. No treated. 44 C 2h. A. B. 44 C 2h + 22 C 24h. GAPDHa Suppresses Heat Shock Induced Cell Death in Arabidopsis Plant. untreated. - PowerPoint PPT PresentationTRANSCRIPT
GAPDHa Suppresses Heat Shock Induced DNA Fragmentation in Arabidopsis plant
No trea
ted
44 C
2h
44 C
2h+22
C 2
4h
No trea
ted
BIP:G
FP
GAPa:GFP
44 C 2h + 22 C 24h
A B
GAPDHa Suppresses Heat Shock InducedCell Death in Arabidopsis Plant
DA
PI s
tain
ed
cell
(%)
BIP:GFP GAPa:GFP
untreated
treated
32
0 32 64 96 128 160 192 224
64
- hea
t shock
+ hea
t shock
Co
un
ts
Heat Shock Induces ROS Accumulation in the Arabidopsis Plant
Heat Shock Induces ROS Accumulation in the Arabidopsis Plant
GAPDHa Suppresses ROS AccumulationInduced by Heat Shock stress
un
tre
ate
d
tre
ate
d
Bright Rh123
E
Nucleoside diphosphate kinase (NDPK)- a hexameric protein composed of 17kDa monomer
- catalyze the reversible transfer of the 5’-terminal phosphates fromtriphosphate nucleosides (or their deoxy derivatives) to nucleoside diphosphates to generate NTPs or dNTPs
- in animals, NDPKs have important role in control of cell proliferation, regulation of transcription, and protein phosphotransferase activity
- in plants, it is associated with phytochrome B response, UV-B signaling, heat stress and hormone related responses
- very little is known about the nature of regulation of NDPK gene in eukaryotes.
YNK is induced by oxidative stress
O OHHO
H
COOH
ClCl
DCFH-DA
DCFH
DCFfluorescence
Measurement of Redox-State in the Cell UsingDichlorofluorescein Diacetate (DCFH-DA)
Cell
H2O2OHO O
COOH
Cl Cl
H
O-C-CH3
COOH
H3C-C-O
Cl
OOO
Cl
% DCF stain F P Lw.t. 7.0 3.5 89.9ynk 10.7 36.0 53.3
w.t.
ynk
YNK Regulates ROS Generation
FL1, Rhodamin123 ; FL2, Propidium iodide
YNK Regulates ROS Generation
w. t. Bax ynk1