gelcount user manual 1.6 · revision 1.6 | april 2016 8 2.2 list of features x simultaneous high...

GelCount User Manual

Revision 1.6 | April 2016 1

User Manual

GelCount™ The leading Colony Counter for Cancer Biologists



Product Documentation and User Manual

Revision 1.6 for GelCount software version 1.2.1.0 and above

Designed and Manufactured by:

Oxford Optronix Ltd. 19 & 21 East Central 127 Olympic Avenue

Milton Park Abingdon OX14 4SA

United Kingdom

t: +44 (0)1235 821 803 f: +44 (0)1235 821 678

Primary website: www.oxford-optronix.com Sales email: [email protected]

Support website: http://www.oxford-optronix.com/support/supp_gelcount.htm

Support email: [email protected]

GelCount™ is registered trademarks of Oxford Optronix Ltd. © Oxford Optronix Ltd. 2016. All rights reserved.



Table of Contents 1. SAFETY INFORMATION 5

1.1 Intended Use.................................................................................. 5 1.2 Contra-Indications .......................................................................... 5 1.3 Symbols ......................................................................................... 5 1.4 Definitions ...................................................................................... 6 1.5 Summary of Warnings.................................................................... 6

2. INTRODUCTION 7

2.1 Introduction .................................................................................... 7 2.2 List of Features .............................................................................. 8 2.3 Supported Platforms ...................................................................... 8 2.4 Minimum Recommended PC Specifications .................................. 8 2.5 Description of Hardware................................................................. 9

3. SETUP AND INSTALLATION 16

3.1 Unpacking and Inspection............................................................ 16 3.2 List of Standard Components....................................................... 16 3.3 Software Installation..................................................................... 16 3.4 Connecting GelCount................................................................... 17

4. SOFTWARE USER’S GUIDE 21

5. CARE OF GELCOUNT 22

5.1 Use............................................................................................... 22 5.2 Storage ........................................................................................ 22 5.3 Cleaning....................................................................................... 22

6. TROUBLESHOOTING AND MAINTENANCE 23

6.1 Troubleshooting ........................................................................... 23 6.2 Obtaining Technical Assistance ................................................... 23 6.3 Returning GelCount ..................................................................... 23 6.4 Park for Transport ........................................................................ 24 6.5 Maintenance................................................................................. 24

7. TECHNICAL SPECIFICATIONS 26

8. WARRANTY 28



Figures

Figure 1: Front view of the GelCount .......................................................... 9 Figure 2: GelCount rear panel..................................................................... 9 Figure 3: Mounting a sample loading tray ................................................. 10 Figure 4: ‘Engaging’ the sample loading tray ............................................ 10 Figure 5: 4-place loading tray for multi-well plates .................................... 11 Figure 6: 24-place loading tray for 35 mm Petri dishes ............................. 12 Figure 7: 12-place loading tray for 50 mm Petri dishes ............................. 13 Figure 8: 4-place loading tray for 100 mm Petri dishes ............................. 13 Figure 9: 8-place tray for Falcon® (model 353108) T25 flasks .................. 14 Figure 10: 6-place tray for Falcon® (model 353082) T25 flasks ................ 14 Figure 11: The transport locking mechanism (released) ........................... 17 Figure 12: The transport locking mechanism (engaged) ........................... 24



1. SAFETY INFORMATION Safety information and operating hints appear throughout this manual in the form of warnings and cautions.

1.1 Intended Use

GelCount is intended for the automated detection, enumeration and analysis of stained or unstained, adherent or non-adherent mammalian cell colonies arising from the gold-standard colony formation assay technique in common use in cancer biology research.

1.2 Contra-Indications

GelCount is purely for laboratory, industrial and research use and is NOT intended for in-vitro diagnostics. GelCount is NOT an in-vitro Diagnostic Medical Device.

1.3 Symbols

SN Serial number REF Catalogue number (product code)

Date of manufacture

Equipment should not be disposed of in the normal waste stream

Read instructions for use

USB output

Device conforms with EU health and safety requirements



1.4 Definitions

WARNING

A warning indicates the possibility of injury to the operator.

CAUTION

A caution indicates a condition that may lead to equipment damage and/or malfunction.

1.5 Summary of Warnings

WARNING

To avoid electric shock and damage to GelCount ensure that no liquids or media are spilled inside the GelCount unit.

WARNING

To avoid electric shock, do not spray, pour or spill any liquid in or on GelCount.

WARNING

To avoid electric shock, the cover of the GelCount unit must only be removed by Oxford Optronix service staff. There are no user-serviceable parts inside.

WARNING

Failure to pack the GelCount appropriately for repair or service is likely to cause costly damage during transit.

WARNING

The GelCount should only be repaired or serviced by Oxford Optronix service staff. There are no user serviceable parts inside.



2. INTRODUCTION

2.1 Introduction

GelCount™ is an innovative, bench-top semi-automated platform for the detection, counting and characterization of stained/adherent or of unstained/non-adherent mammalian cell colonies in soft agar and other semi-solid media and suspension cultures.

The GelCount is primarily intended for use in conjunction with the colony forming assay as employed by cancer biologists in specialty fields such as,

x Radiation Biology x Chemotherapeutic Research x Drug discovery x Toxicology

GelCount is a cost-effective and reliable alternative to the laborious task of counting colonies manually under a light box or microscope. Since it is both objective and consistent it eliminates the human error factor, which may arise from subjective interpretation, bias and fatigue.

By combining high resolution imaging with high depth-of-field optics and innovative digital image processing using a proprietary multi-thread aware CHARM II™ algorithm, GelCount can rapidly detect individual colonies as small as 30 µm in diameter and differentiate real colonies from debris and artifacts.

GelCount is conceived for the processing of both adherent, stained colonies and for non-adherent, stained or unstained colonies in semi-solid media or in suspension culture. Supported plasticware includes multi-well plates, Petri dishes and T25 flasks.

The Windows®-based operating software delivers excellent, user-configurable colony detection performance, a wide range of statistical outputs exported directly into Excel® and a choice of image archiving/exportation options.

In use, the GelCount unit is connected to a PC via a high-speed USB2 interface. The GelCount is controlled from the PC via the operating software.



2.2 List of Features

x Simultaneous high resolution and high depth-of-field imaging capability.

x CHARM II™ image processing algorithm for accurate and objective counting of colonies, including overlapping colonies.

x Motorized drawer and tray loading mechanism supports standard 6-, 12-, 24-, 48- or 96-well plate formats, 35, 50 or 100 mm Petri dishes and T25 flasks.

x USB 2.0 interface to PC. x Multi-thread aware software for simultaneous imaging and image

processing. x Fully user-customisable counting area / mask. x Fully user-definable inclusion/exclusion of colonies based on colony

size, shape and optical density thresholds. x Colony statistics outputs, including colony diameter, colony density

and colony nearest neighbour distance parameters. x Extensive raw data and image archiving functionality, including auto-

export to Microsoft® Excel®. x Robust and ergonomic design.

2.3 Supported Platforms

The GelCount operating software requires a PC running Windows XP, Windows 7, or Windows 10. Mac OS X and the Apple PC platform is NOT presently supported. However the GelCount software can be run in ‘off-line’ image analysis mode on Mac computers featuring Intel® processors via the Apple Boot Camp application (or third-party virtualisation software) where Windows 7 or Windows 10 is installed.

2.4 Minimum Recommended PC Specifications

2.0 GHz dual-core processor, 2 GB system memory, CD-ROM, USB 2.0 interface (2 ports required), Windows XP, Windows 7, or Windows 10, dedicated graphics accelerator with 256 MB or above of video memory (or new-generation ‘APU’ processors with on-die GPU from Intel/AMD), Microsoft® Excel® installed.



2.5 Description of Hardware

1

2

3

Figure 1: Front view of the GelCount 1. Power-on indicator light 2. Open/Close drawer switch 3. Motorized drawer (runners), holding 4-way plate loading tray (fully engaged)

Device serial number USB ports On/Off power switch and IEC power inlet connector Fuse compartment

Figure 2: GelCount rear panel



Sample loading trays are mounted along the silver runners in the direction indicated

Figure 3: Mounting a sample loading tray To mount a sample loading tray, mount it on and carefully slide it along the silver runners in the direction indicated. All loading trays are asymmetric to ensure only one orientation is possible (see figures 5 - 9 below).

Not fully engaged

Fully engaged

Figure 4: ‘Engaging’ the sample loading tray Resistance will be felt when the tray is almost flush with the ends of the runners. Push against the resistance and the tray will drop a fraction to fully engage within the runners, ensuring a secure hold. Tug briefly to confirm that the tray is engaged; it should not move backwards.

To remove a loading tray, gently lever the tray upwards to disengage it before reversing it out along the runners.

NOTE: Loading trays MUST be in the fully engaged position to ensure positional reproducibility and thus optimum imaging/counting performance.



FRONT

Grub screws for spring-loaded bearings; use Allen key provided to adjust, ensuring a secure plate fit

Product code: ‘PLATE_TRAY’

Figure 5: 4-place loading tray for multi-well plates

Supports 6-well, 12-well, 24-well, 48-well and 96-well plates. The plate tray may be loaded with between 1 and 4 plates, respecting the positional order indicated. Adjustable, spring-loaded bearings are designed to ensure sample plates are securely and reproducibly positioned and pressed towards the centre of the tray; use the Allen key supplied to adjust bearings as necessary. Care should be taken to ensure plates are seated flat within the inner ‘rim’ of each placement. To release culture plates from the plate tray, gently push from beneath the plate. Plate lids can be left in place prior to imaging where sterility is required, however heavy condensation should be avoided/removed.

1 2

3 4



FRONT

Product codes: ‘PETRI_TRAY_35’ (for Nunc™, Greiner™ and most other makes of 35 mm Petri dishes). ‘PETRI_TRAY_35_F’ (for Falcon™ 35 mm Petri dishes).

Figure 6: 24-place loading tray for 35 mm Petri dishes

Two variants available: For Nunc™, Greiner™ and most other makes of 35 mm Petri dishes (product code ‘PETRI_TRAY_35’), For Falcon™ 35 mm Petri dishes (product code ‘PETRI_TRAY_35_F’). The tray may be loaded with between 1 and 24 Petri dishes, respecting the positional order indicated. Care should be taken to ensure Petri dishes are seated flat within the inner ‘rim’ of each placement. Lids can optionally be left in place prior to imaging where sterility is required, however heavy condensation should be avoided/removed.

7 8 9 10 11 12

13 14 15 16 17 18

19 20 21 22 23 24

1 2 3 4 5 6



FRONT

Product code: ‘PETRI_TRAY_50’

Figure 7: 12-place loading tray for 50 mm Petri dishes The tray may be loaded with between 1 and 12 Petri dishes, respecting the positional order indicated. Care should be taken to ensure Petri dishes are seated flat within the inner ‘rim’ of each placement.

FRONT

Product code: ‘PETRI_TRAY_100’

Figure 8: 4-place loading tray for 100 mm Petri dishes The tray may be loaded with between 1 and 4 Petri dishes. Care should be taken to ensure Petri dishes are seated flat within the inner ‘rim’ of each placement.

NOTE: Also available is a purpose-designed tray for Eppendorf® model ‘003 070 2115’ 100 mm Petri dishes (product code PETRI_TRAY_100_E).

7 8

9 10 11 12

1 2 3 4

5 6

1 2

3 4



FRONT

Product code: ‘T25_TRAY_FLCN_T2’

Figure 9: 8-place tray for Falcon® (model 353108) T25 flasks The flask tray may be loaded with between 1 and 8 T25 flasks, respecting the positional order indicated. Care should be taken to ensure flasks are seated flat within the inner ‘rim’ of each placement.

IMPORTANT: Falcon® (model 353108) flasks supported ONLY. Flask caps MUST be removed prior to imaging.

FRONT

Product code: ‘T25_TRAY_FLCN’

Figure 10: 6-place tray for Falcon® (model 353082) T25 flasks IMPORTANT: Falcon® model 353082 T25 flasks supported ONLY. Flask caps MUST be removed prior to imaging.

7 8

1 2

3 4

5 6

1 2

3 4

5 6



WARNING

For T25 flasks, caps MUST be removed prior to imaging. Damage to the GelCount may result.

CAUTION

Multi-well plates, Petri dishes or flasks must be positioned such that all edges sit flush against the protruding rim of the loading tray.

CAUTION

Loading trays should be placed flat on a solid work surface before being loaded with multi-well plates or Petri dish samples.

CAUTION

Sample plates, Petri dishes and lids must be transparent, clean and completely free of markings.



3. SETUP AND INSTALLATION

3.1 Unpacking and Inspection

Immediately notify the shipping courier and Oxford Optronix Ltd. or your local distributor if the outer packaging or carton is wet or damaged in any way. Unpack the GelCount and its components. If anything is missing or damaged contact Oxford Optronix Ltd. or your local distributor. NOTE: We recommend that the original shipping carton and sponge inserts be stored in a safe place rather than discarded, since these will be required for any warranty returns and/or for shipping the GelCount safely at recommended servicing intervals.

3.2 List of Standard Components

x GelCount Unit x IEC power cable for your country x USB 2.0 interface cables (x 2) x Software CD-ROM x User Manual (this document) x Software User’s Guide x One or more sample loading trays x Allen key to adjust bearing grub screws on multi-well plate tray

3.3 Software Installation

IMPORTANT: Install the GelCount software BEFORE connecting the GelCount to the PC.

1. Please confirm that your PC meets the recommended minimum specifications listed in section 2.4 above.

2. Log on as administrator or as a user with ‘administrator rights’. 3. Insert the GelCount software CD into the CD/DVD drive. 4. Windows should automatically launch the installation routine after a

short delay. If not, right-click on the CD/DVD drive in ‘My Computer’ and select ‘Autorun’, or double-click on ‘GelCount_Setup.exe’ in the root folder of the installation CD.

5. Follow the on-screen installation directions, including installation of device drivers (see note below), as prompted.



NOTE: For users wishing to install the software on a workstation PC that will NOT directly be connected to the GelCount (i.e. be used purely for ‘off-line’ image processing), there is NO requirement to install the device drivers. Select ‘Cancel’ when prompted to install drivers (following the program installation step).

3.4 Connecting GelCount

IMPORTANT: Ensure the GelCount software is installed BEFORE connecting the GelCount to the PC.

1. Position the unit on a flat and stable surface, ensuring there is nothing to obstruct the movement of the motorized drawer in front of the unit.

2. Turn the transport locking mechanism into the RELEASED position, as shown.

Figure 11: The transport locking mechanism (released) The locking mechanism is found beneath the front, right-hand corner of the GelCount. Use a flat-head screw driver or a suitable coin to turn the transport locking mechanism into the RELEASED position.

3. Connect the IEC mains lead to the wall mains supply and to the

mains inlet at the rear of the GelCount and power up the GelCount using the power on/off switch. The GelCount will auto-detect the local mains voltage (110/120V or 220/240V supported), the blue panel on the top of the unit will illuminate and the device will make various noises as internal moving parts are initialized.



4. Connect the USB cable labelled “1” (ONLY) to the port labelled “1” at the rear of the GelCount and to any available USB port on the PC. Windows 7 and Windows 10: Windows 7 and Windows 8 should automatically recognize the new device. In Windows 7 a “Installing device driver software” pop-up will be followed after a short delay by a pop-up confirming the device name. In Windows 8 a temporary, animated taskbar icon will provide feedback on the progress of device recognition. Windows XP: Windows XP should automatically detect and install the new device. An on-screen pop-up will confirm that is ready for use after a short delay. If instead Windows launches the ‘Found New Hardware’ wizard, please proceed as follows:

Step 1 If prompted to connect to Windows Update, select ‘No, not this time’ and click ‘Next’. Step 2 (depicted) Select 'Install from a list of specific locations (Advanced)' and click 'Next'.

Step 3 (depicted) Select 'Search for the best driver in these locations', un-tick ‘Search removable media..’, tick ‘Include this location in the search:’ and browse to ‘Local Disk (C:)’ > ‘Program Files’ > ‘GelCount’ > ‘Drivers’ > ‘stage’ and click 'Next'. On the final panel click ‘Finish’ to complete installation of the ‘USB Serial Converter’ device.



5. Connect the USB cable labelled “2” to the port labelled “2” at the rear of the GelCount and to any available USB port on the PC. Windows 7 and Windows 8: Windows 7 and Windows 8 should automatically recognize the new device. In Windows 7 a “Installing device driver software” pop-up will be followed after a short delay by a pop-up confirming the device name. In Windows 8 a temporary, animated taskbar icon will provide feedback on the progress of device recognition. Windows XP: Windows XP should detect and install the imaging device automatically and confirm after a short delay that it is ready to use. If Windows launches the ‘Found New Hardware’ wizard, please proceed as follows:

Step 1 If prompted to connect to Windows Update, select ‘No, not this time’ and click ‘Next’. Step 2 (depicted) Select 'Install from a list of specific locations (Advanced)' and click 'Next'.

Step 3 (depicted) Select 'Search for the best driver in these locations', un-tick ‘Search removable media..’, tick ‘Include this location in the search:’, browse to ‘Local Disk (C:)’ > ‘Program Files’ > ‘GelCount’ > ‘Drivers’ > ‘imager1’ and click 'Next'. On the final panel click ‘Finish’ to complete installation of the imaging device.



6. Installation is now complete; the GelCount software can be launched from the desktop shortcut icon or program group. NOTE: Should errors be displayed on launching the GelCount software, please quit the software and re-boot the PC with the GelCount connected and turned on, then try launching once more.

7. Press the open/close drawer button to extend the drawer in preparation to receive the loading tray, as described above in figures 3 to 9. Refer to the ‘Software User’s Guide’ (supplied at the time of shipment and available from the Help menu) for software operating instructions.

CAUTION

For optimal reliability the GelCount should be turned on PRIOR to turning on the PC.



4. SOFTWARE USER’S GUIDE The Software User’s Guide document is supplied in printed form at the time of delivery and can be invoked as a PDF document from the software Help menu.



5. CARE OF GELCOUNT

5.1 Use

GelCount should only be operated on a secure, flat, horizontal surface and between 15°C to 30°C.

WARNING

To avoid electric shock and damage to GelCount, ensure that no liquids or media are spilled inside the GelCount unit.

5.2 Storage

GelCount should be stored between 10°C to 40°C. When returning from extremes of temperature, the unit should be allowed to acclimatise at room temperature for 30 minutes before use.

5.3 Cleaning

WARNING

To avoid electric shock, do not spray, pour or spill any liquid in or on the GelCount.

The GelCount enclosure and the loading trays may be cleaned using a soft cloth dampened with a commercial, non-abrasive cleaner and/or disinfected with 70% alcohol in water.



6. TROUBLESHOOTING AND MAINTENANCE 6.1 Troubleshooting

If you experience a problem with the GelCount please contact Oxford Optronix technical support or your local distributor.

6.2 Obtaining Technical Assistance

Contact information for obtaining technical assistance is provided at the beginning of this manual and on the Oxford Optronix web site.

When contacting Oxford Optronix technical support or your local distributor please provide the Serial Number of your GelCount unit (found at the rear of the unit), the version of GelCount software in use (select ‘About’ from the Help menu) and details of the error message encountered (if any).

6.3 Returning GelCount

Contact Oxford Optronix or your local distributor for shipping instructions. A Returned Materials Authorisation (RMA) number and completed Decontamination Declaration Form MUST be obtained prior to shipping the equipment.

Prior to packing/shipping it is essential to remove any loading tray from within the GelCount.

Prior to packing/shipping it is essential that the transport locking mechanism is engaged to safeguard the moving parts within the GelCount - see next section.

Pack GelCount in its original shipping carton where available. If the original carton is not available, please contact Oxford Optronix to request original packing materials that will ensure safe transportation.

Use a reputable courier company for the return of product to Oxford Optronix (UPS, FedEx etc).

WARNING

Failure to pack the GelCount appropriately for repair or service is likely to cause costly damage during transit.

Oxford Optronix will not accept responsibility for any loss or damage to goods shipped to us howsoever caused.



6.4 Park for Transport

Prior to shipping the GelCount by courier it is essential that the transport locking mechanism is engaged to safeguard the moving parts within the GelCount.

With the GelCount switched on and connected to the PC, launch the GelCount software and select ‘Park for Transport’ from the ‘Advanced’ menu. Enter the numerical ‘Park Position’ provided on the label attached next to the locking mechanism (located on the underside of the GelCount, beneath the front-right hand corner - depicted below) and click OK.

Once the motorized drawer has moved to its allocated position, quit the software, turn off the GelCount, remove both the mains and USB cables and turn the locking mechanism to the ENGAGED position, as shown.

Figure 12: The transport locking mechanism (engaged) The locking mechanism is found beneath the front, right-hand corner of the GelCount. Use a flat-head screw driver or a suitable coin to turn the transport locking mechanism into the ENGAGED position.

The GelCount is now safe to pack and ship.

6.5 Maintenance

It is recommended that GelCount be serviced every 2 years. Units in heavy (daily) use may benefit from annual servicing. Servicing includes the implementation of hardware upgrades introduced as part of our continuous product improvement program, ensuring optimal performance and reliability. Please contact Oxford Optronix Ltd. for a full service schedule and pricing. Extended warranty service contracts are also available to purchase.



WARNING

Prior to packing/shipping it is essential to remove the loading tray from within the GelCount.

WARNING

Prior to shipping the GelCount it is essential that the transport locking mechanism is engaged to safeguard the moving parts within the GelCount (see section 6.4).

WARNING

To avoid electric shock, the cover of the GelCount unit must under no circumstances be removed.

WARNING

The GelCount must only be repaired or serviced by Oxford Optronix service personnel. There are no user serviceable parts inside.



7. TECHNICAL SPECIFICATIONS

Physical

Imaging method High resolution 16-bit greyscale CCD line imager, visible light trans-illumination

Imaging resolution 300 dpi - 2,400 dpi, user selectable Depth of field 0 - 5 mm effective, above well base Sample loading method Removable loading tray, latching into a

software-controlled motorized drawer Plasticware supported Multi-well plates (6, 12, 24, 48 and 96-well

plates with/without lids; 35, 50 and 100mm Petri dishes; selected T25 flasks

Loading tray capacity Up to 4 multi-well plates; up to 24 Petri dishes; up to 8 T25 flasks

PC interface USB 2.0 (x2) Dimensions 155mm x 560mm x 450mm (H x W x D) Weight 20 kg Power requirements 100 - 240V ~ 1.5A, 50-60 Hz; 2 x T1.6A fuse Storage temperature 10 - 40°C Operating temperature 15 - 30°C Operating humidity 0 - 70% (non condensing)

Performance

Minimum resolvable colony diameter

Approx. 30 µm (at 2,400 dpi image resolution)

Typical acquisition time 12 minutes (four 6-well plates at 1,200 dpi) Colony detection CHARM II™ (Compact Hough and Radial

Map) image processing algorithm, proprietary to Oxford Optronix Ltd., dedicated to colony detection and size characterization.



Colony types supported Adherent: stained (methyl blue, crystal violet, or equivalent). Non-adherent in soft agar, methylcellulose or other semi-solid media: unstained or stained (MTT or equivalent).

Counting variability < 5% for repeated analysis of the same sample

Numerical data output Automated exportation to Excel® of colony counts, mean colony diameter, area, volume and other ‘statistics’ per well/dish. Optional ‘csv’ output of per-colony data and/or statistics histograms.

Image output Per well/dish or compound bitmap images for general purpose importation/printing. Per well/dish raw images for ‘off-line’ image analysis and/or archiving.

Minimum PC Requirements See section 2.4



8. WARRANTY Oxford Optronix Ltd. warrants its Products against defects in material or in workmanship, when used under appropriate conditions and in accordance with the appropriate Operating or User Instructions for a period of 24 months from the date of purchase. Oxford Optronix’ sole obligation shall be to repair or to replace at Oxford Optronix’ option, F.O.B. its factory, without charge, any part(s) that prove defective within the warranty period. Software programs are supplied on the strict understanding that we do not warrant their functions to be free from defects, errors or bugs. Any claim under the warranty must be made in writing. The Products to which the claim refers must be returned to us within 2 months from the date the claim was made, suitably packaged, using our Returned Materials Authorisation (RMA) procedure and our courier (e.g. FedEx) account reference. No returned Products will be accepted without prior written authorisation and an RMA number. Oxford Optronix Ltd. is not liable under this warranty: - for any defect arising from fair wear and tear, wilful damage, negligence, misuse, repair of the Products without our written approval; or - any use of or dealing with the Products in conjunction with any other item where such item causes or gives rise to the alleged defect; or - any use of the Products which is not in accordance with the Operating or User Instructions or from any failure to service or maintain the Products in accordance with such instructions. Oxford Optronix Ltd. specifically disclaims any other express or implied warranty, including warranties of merchantability and of fitness for use. To the full extent permitted by law, we shall not be liable in any way whatsoever whether in contract, in tort, in misrepresentation or under statute or common law or otherwise (whether caused by our negligence or otherwise) in respect of defects in the Products or failure to correspond to specification or for any injury, damage or loss resulting from such defects or failure. In no event shall any breach of contract on our part or tort (including negligence) or failure of any kind on our part give rise to any liability for loss of revenue or loss of profits or any other consequential or indirect loss or damage arising from any cause whatsoever.

gelcount user manual 1.6 · revision 1.6 | april 2016 8 2.2 list of features x simultaneous high...

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