gene doping of mice with pgc1a via ormosil to produce fitness kamran rowshandel
TRANSCRIPT
PGC1a
• Causes mitochondrial biogenesis
• Prevents ROS-mediated degradation and other damage to mitochondria by signaling for the expression of a slew of proteins that keep mitochondria healthy
• Expressed every time you exercise!
Ormosil
• Ormosil is organically modified silica and has many purposes due to physical characteristics it possesses due to the way it is on a “nano” level
• There are multiple ways of getting a gene to be expressed by an animal, but some methods have drawbacks
• Ormosil does not have drawbacks compared to viral gene delivery vectors, which have killed people in the past
The method
• Ormosil vectors are made by mixing the gene and ormosil together in solution and letting sit for 30 minutes
• IV injection into adult mice yields expression of the vector
Naturally,
• A group of 60 adult mice will be injected with the treatment
• A group of 40 adult mice will exercise daily on motorized treadmills
• And a group of 20 adult mice will be sedentary
Around the world in 80 days
• Groups will be examined for indicators of fitness such as muscle development by visual appearance on a daily basis as they are fed and taken care of
• On the final day all mice will be run on motorized treadmills to exhaustion