GENRES Leafy Veg Project

1st Annual project meeting in Angers, France

12th & 13th December 2007

Partner 1 – Warwick HRIInputs in year 1

April 2004 became the Plant and Crop Science Research Research Department of the Department of the University of WarwickUniversity of WarwickSites at Wellesbourne, Sites at Wellesbourne, Warks. and Kirton, Lincs.Warks. and Kirton, Lincs.

Modern Modern LaboratoriesLaboratories

6500m6500m22 Computer Controlled Computer Controlled GlasshousesGlasshouses

Controlled Controlled Environment facilitiesEnvironment facilities

Wellesbourne campusWellesbourne campus

192 hectares of 192 hectares of landland Bioconversion Bioconversion

unitunit

Genetic Resources Genetic Resources UnitUnit

Funded by

International Board for Plant International Board for Plant Genetic Resources Base Genetic Resources Base

CollectionsCollectionsAlliumAlliumBrassicaBrassicaDaucusDaucusLactucaLactucaRaphanusRaphanus

>13,800 accessions in >13,800 accessions in long term storage at -20long term storage at -2000CC

WP1 DocumentationStart-up meeting agreed partners to decide material for regeneration, characterization and evaluation.P1 completed the selection of material for regeneration (lettuce, chicory & spinach), characterization (lettuce & spinach) and evaluation (lettuce for post harvest discolouration).P1 provided passport data for lettuce, chicory, spinach & Eruca to the relevant database managers.Characterization data were recorded for lettuce and spinach in the formats provided by the database managers.

WP2 Regeneration & CharacterizationWP2 Regeneration & Characterization RegenerationSeed produced for 31 accessions of leafy veg following best local practice. Lettuce (20 accessions) grown in GH with inflorescences isolated using bread bags.Spinach (5 accessions) isolated in sealed GH compartments to prevent cross pollination via air-borne pollen.Chicory (6 accessions) isolated in insect-proof cages in GH with flies introduced as pollinators.

Threshed clean seeds were dried to 5% moisture content based on standard ISTA test & currently are being packed in foil for long term storage & safety duplication.

WP2 Regeneration & CharacterizationWP2 Regeneration & Characterization Characterization Seeds sown in modules and thinned with individuals transplanted & grown on in a Dutchlight frame.

Agreed characters were scored & images captured. Data were recorded in the agreed format for each crop. Lettuce - 10 accessions of Cos typeSpinach - 10 accessions

Lettuce crop Lettuce crop improvement researchimprovement research

Quality of Processed saladQuality of Processed salad

WP3 EvaluationAll 24 lettuce accessions identified for inclusion in the screen for post harvest discolouration (browning/pinking) were grown in year 1. Plants harvested at commercial maturity were evaluated following a standard protocol by PhD student Laura Atkinson

“Pinking”

Day 1 Ang %P Lsd shown at 5% level

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Day 13 Ang %P Lsd shown at 5% level

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Day 1 Ang %B Lsd shown at 5% level

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Day 13 Ang %B Lsd shown at 5% level

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“Browning”

WP4 UtilizationWP coordinationWP5 Project managementAttend project meetings; WP coordinators meetings;Write WP annual report!

Thank youThank you