George TwaddleProgram Chair for BiotechnologyIvy Tech Community College of Indiana – South Bend

The Ultimate Inquiry

Lessons from the

Genetically Engineered Machine

Competition

How to get students interested in STEM careers?

Gibson Assembly Song

Team Cambridge iGEM Competition 2010

Synthetic Biology• New area of biological

research

• Combines science and

engineering

• Design and construction of

artificial biological functions and systems

Genetic Code is UNIVERSAL

-Discovery of “restrction enzymes” allows us to cut and build recombinant DNA and to express new genetic combinations..

The New Generation of “Micro” Computers?

Began in January of 2003Independent Activities Period (IAP) at MIT (The students designed biological systems to make cells blink).

A BIT O’ iGEM HISTORY

5 teams in 2004 13 teams in 200532 teams in 2006 54 teams in 2007 84 teams in 2008 112 teams in 2009 130 teams in 2010

2011.igem.org

The International Genetically Engineered Machine competition (iGEM) is the premiere undergraduate Synthetic Biology competition. Student teams are given a kit of biological parts at the beginning of the summer from the Registry of Standard Biological Parts. Working at their own schools over the summer, they use these parts and new parts of their own design to build biological systems and operate them in living cells. This project design and competition format is an exceptionally motivating and effective teaching method.

What’s an “iGEM?”

Kit of biological parts

at the beginning of

the summer.

Build biological systems

out of the parts or new

parts that they create and

operate them in living cells

Come to a “Jamboree”

at the end to show-off

and see who is most innovative.

An undergraduate synthetic biology annual competition

Tom Knight“BioBrick” inventorGinko BioWorks

Randy RettbergiGEM Competition“Open WetWare” Drew Endy

BioBricks Foundation

iGEM MIT Paternity

THE “BIOBRICK”A standard for interchangeable parts.

For building complex biological systems in cells

Standards achieved through unique “prefix” and “suffix” restriction sites.

A3 Assembly of BioBricks to Form a “Device”

Student created wiki part page

Elements of the Competition

Team wiki and Team Parts Pages

Poster presentation

Platform presentation

•Track Award Winners: Best Food or Energy Project: BCCS-Bristol•Best Environment Project: Peking•Best Health or Medicine Project: Washington & Freiburg Bioware (Tie)•Best Information Processing Project: ETHZ Basel & Tokyo Tech (Tie)•Best Manufacturing Project: MIT•Best New Application Area: Slovenia•Best Foundation Advance: Paris Liliane Bettencourt•Best Software Tool: USTC Software

•Special Prizes Winners: Best BioBrick Part, Natural: Minnesota (BBa_K311004)•Best New BioBrick Part or Device, Engineered: Slovenia (BBa_K323135)•Best Human Practices Advance: Imperial College London•Safety Commendation: SDU Denmark & VT-ENSIMAG Biosecurity (Tie) •Best Experimental Measurement: Duke•Best Model: Edinburgh•Best Wiki: Cambridge & Imperial College London (Tie)•Best Poster: KIT-Kyoto•Best Presentation: TUDelft•iGEMers Prize: Cambridge, Imperial, MIT, EPF Lausanne, & Slovenia

Medals and Awards

GoldSilverBronze

Inquiry-Based Learning“A student-centered / teacher-guided instructional approach that

Engages students in investigating

Real World Questions that they choose within a broad thematic framework.

Students acquire and analyze information, develop and support propositions, provide solutions, and design technology that demonstrate their thinking and make their learning visible.” Northeastern Illinois University Dept. of Eduction

What’s your Problem?Food?Energy?

Environment?Health and Medicine?

Industrial & Biomanufacturing?

Software?

Process Advance?

Team Project Description

Aberdeen AyeSwitch Genetic toggle

BCCS-Bristol agrEcoli Soil Fertility Sensor

Brown Light Pattern Control of Cell Circuits

Caltech Bioplastic printer from soybeans and E. coli

EFP-Lusanne Asaia GM bacteria antimalarial

ETHZ-Basel E. Lemming Remote contolled E. coli

Gronigen Hydrobiophilm Synthetic hydrophobic surface

Harvard iGarden Plant Engineering Toolkit

HokkaidoU_Japan Dr. E.coli Protein microinjector

IvyTech-SouthBend To Swim or Not to Swim

P. Aeruginosa biosensor

Lethbridge Bioremediation of tailing ponds

Mexico Very Cool E. coli

Produce antifreeze ,15C

Missouri_Miners Electric E. coli E.Coli Fuel Cell

Newcastle BacillaFilla Bacteria concrete crack filler

UT-Tokyo E. Coli Solves Sudoku

Team Groningen, Netherlands2009

Team British Columbia, Canada 2009

EPFL Luasanne, Switzerland 2010

University of Slovenia 2010

Ivy Tech Community College of IndianaBronze Medal Team

    

EducationCommunit

y Industry

Local Gov

State Gov.

Federal Gov.

Foundations

Media

BioFuture: Partnerships in Biotechnology

Partnerships in the Future

   

SKILLS AND KNOWLEDGE STANDARDS FORASSOCIATE DEGREE IN BIOTECHNOLOGY PROGRAMS

IN INDIANA  

Created by:Alisa Wright, Baxter Pharmaceutical SolutionsGary Bormett, Dow AgroSciences LCCJose Hanquier, Robert MacKay, Roberta Talmage, Eli Lilly and CompanyWade Lange, Indiana Health Industry ForumAndy Cothrel, Roche Diagnostics Corp.   June 30, 2003

Associate of Science orAssociate of Applied Science

In

BIOTECHNOLOGYBloomington Lafayette Lawrence South Bend Terra Haute

iGEM High School Division 2010

• Greenfield Central High School• Warren Central High School• “The DNA Architects of South Bend – Mishawaka (Marian, Penn and Riley High Schools

Illustration creditsLine of students: www.LowestPricedTrafficSchool.comGibson Assembly Song: www.cambrigeigem.orgSynthetic biology figures: “Biology” Campbell and Reece. Pearson, Benjamin-Cummings Publishers (2010) www.masteringbiology.comBacteria: Ministry of Public Works, Canada www.blog.poolcenter.comiGEM team photos, wiki screen shots, etc. 2011.igem.org