gh sensitivity dna assay de72905009 2016-03-07 11-52-27 ... · 3/7/2016 · 2100 expert_high...

Instrument Name: DE72905009 Firmware:Serial#:Assay Information:

C.01.069DE72905009

Instrument Information:

Assay Origin Path: C:\Program Files (x86)\Agilent\2100 bioanalyzer\2100expert\assays\dsDNA\High Sensitivity DNA.xsy

Assay Class:Version:Assay Comments:

High Sensitivity DNA Assay1.03Copyright © 2003-2010 Agilent Technologies

Chip Information:

Chip Comments:

Type: G2938C

Chip Lot #:Reagent Kit Lot #:

blank SmitT-17M SmitT-27M

ShafB-35 ShafB-1M ShafB-1B

blank blank blank

blank blank

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 3/7/2016 12:59:08 PM

2100 expert_High Sensitivity DNA Assay_DE72905009_2016-03-07_11-52-27.xad Page of1 6

Created:Modified:

3/7/2016 11:52:26 AM3/7/2016 12:56:47 PMData Path:

High Sensitivity DNA AssayC:\...gh Sensitivity DNA Assay_DE72905009_2016-03-07_11-52-27.xad

Assay Class:

Electrophoresis File Run Summary

Smith Lab

Typewritten Text

=BestRad, Butterfly P2

Smith Lab

Typewritten Text

=RAD, Butterfly P2

Sample Name SampleComment

Rest.Digest

Status

Observation ResultLabel

Result Color

blankSmitT-17MSmitT-27MShafB-35ShafB-1MShafB-1BblankblankblankblankblankLadder

Chip Lot # Reagent Kit Lot #

Chip Comments :



Created:Modified:

3/7/2016 11:52:26 AM3/7/2016 12:56:47 PMData Path:


Assay Class:

Electrophoresis File Run Summary (Chip Summary)

General Analysis SettingsNumber of Available Sample and Ladder Wells (Max.) : 12Minimum Visible Range [s] : 32Maximum Visible Range [s] : 138Start Analysis Time Range [s] : 33End Analysis Time Range [s] : 137.5Ladder Concentration [pg/µl] : 1950Uses Standard Area for Ladder FragmentsLower Marker Concentration [pg/µl] : 125Upper Marker Concentration [pg/µl] : 75Used Upper Marker for QuantitationStandard Curve Fit is Point to PointShow Data Aligned to Lower and Upper Marker

Integrator SettingsIntegration Start Time [s] : 33.05Integration End Time [s] : 137Slope Threshold : 0.8Height Threshold [FU] : 5Area Threshold : 0.1Width Threshold [s] : 0.6Baseline Plateau [s] : 0.5

Filter SettingsFilter Width [s] : 0.5Polynomial Order : 4

Ladder Ladder Peak Size Area1 35 1602 50 2103 100 2084 150 2215 200 2426 300 2707 400 3058 500 3069 600 33610 700 32111 1000 36612 2000 41313 3000 41114 7000 40015 10380 214



Created:Modified:

3/7/2016 11:52:26 AM3/7/2016 12:56:47 PMData Path:


Assay Class:

Electrophoresis Assay Details

Peak table for sample 2 : SmitT-17MPeak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations1 35 125.00 5,411.3 Lower Marker2 89 8.12 138.73 126 104.88 1,257.24 348 396.81 1,727.45 384 353.67 1,393.96 408 207.53 770.37 421 122.33 440.78 431 139.44 490.79 451 114.99 386.210 469 211.78 684.911 493 647.22 1,987.812 735 29.23 60.213 835 23.32 42.314 962 64.40 101.415 1,356 30.86 34.516 1,902 2.15 1.717 2,505 1.96 1.218 10,380 75.00 10.9 Upper Marker19 13,186 0.00 0.0Region table for sample 2 : SmitT-17MFrom[bp]

To [bp] Corr.Area

% ofTotal

Average Size[bp]

Size distribution inCV [%]

Conc.[pg/µl]

Molarity[pmol/l]

Color

200 2,755 3,259.5 94 526 51.4 2,484.23 8,506.1

SmitT-17M

Overall Results for sample 2 : SmitT-17MNumber of peaks found: 17 Noise: 0.3

Corr. Area 1: 3,259.5



Created:Modified:

3/7/2016 11:52:26 AM3/7/2016 12:56:47 PMData Path:


Assay Class:

Electropherogram Summary

Smith Lab

Typewritten Text

= BestRad, Butterfly P2

Peak table for sample 3 : SmitT-27MPeak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations1 35 125.00 5,411.3 Lower Marker2 277 140.86 770.13 314 222.98 1,074.34 387 515.40 2,018.95 403 69.03 259.76 424 158.03 565.07 443 75.58 258.48 457 79.49 263.89 468 721.05 2,332.310 1,373 4.92 5.411 1,496 4.50 4.612 1,635 4.37 4.013 1,736 4.51 3.914 2,183 4.43 3.115 2,556 2.73 1.616 2,883 2.81 1.517 3,259 2.96 1.418 3,977 3.09 1.219 5,612 1.33 0.420 6,330 1.42 0.321 10,380 75.00 10.9 Upper MarkerRegion table for sample 3 : SmitT-27MFrom[bp]

To [bp] Corr.Area

% ofTotal

Average Size[bp]

Size distribution inCV [%]

Conc.[pg/µl]

Molarity[pmol/l]

Color

200 1,000 3,100.2 95 442 27.5 2,076.16 7,843.9

SmitT-27M

Overall Results for sample 3 : SmitT-27MNumber of peaks found: 19 Noise: 0.3

Corr. Area 1: 3,100.2



Created:Modified:

3/7/2016 11:52:26 AM3/7/2016 12:56:47 PMData Path:


Assay Class:

Electropherogram Summary Continued ...

Smith Lab

Typewritten Text

= RAD, Butterfly P2



Created:Modified:

3/7/2016 11:52:26 AM3/7/2016 12:56:47 PMData Path:


Assay Class:

Gel Image

Smith Lab

Typewritten Text

gh sensitivity dna assay de72905009 2016-03-07 11-52-27 ... · 3/7/2016 · 2100 expert_high...

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