glyphosate in mmr: does this explain the au7sm link? · wheat, oats, barley, rye, sugar cane,...
TRANSCRIPT
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GlyphosateinMMR:DoesThisExplaintheAu7smLink?
StephanieSeneffCSAIL,MIT
Tuesday,Sept.19,2017
“Itisdangeroustoberightinma2ersonwhichtheestablishedauthori6esarewrong.”
–Voltaire
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Outline
• Whatisglyphosate?• Howglyphosateaffectsthegutbarrier• Glyphosateinvaccines– GlutamateandglyphosatesynergyinMMR
• Autoimmunediseasethroughmolecularmimicry• Summary
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RoundupandGMOCrops
GMORoundup-Readycorn,soy,canola,sugarbeetscoOon,tobaccoandalfalfa
Whatisglyphosate?
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RoundupasaDesiccant/RipenerjustbeforeHarvest
Wheat,Oats,Barley,Rye,Sugarcane,Beans,LenSls,Peas,Flax,Sunflowers,Pulses,ChickPeas
*Figure15,Seneffetal.,AgriculturalSciences,2015,6,42-70
Au7smPrevalence:6yearolds*
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IsGlyphosateNontoxic?• Monsantohasarguedthatglyphosateisharmlesstohumansbecausewedon’thavetheshikimatepathwaywhichitdisruptsinplants
• However,ourgutbacteriaDOhavethispathway– TheyuseittosupplyuswithessenSalaromaScaminoacids(precursorstoneurotransmiOers,thyroidhormoneandBvitamins)
• OtheringredientsinRoundupgreatlyincreaseglyphosate’stoxiceffects
• InsidiouseffectsofglyphosateaccumulateoverSme– Moststudiesaretooshorttodetectdamage
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GlyphosateandtheGut
• GlyphosatehasbeenfoundasacontaminantindigesSveenzymestrypsin,pepsinandlipase*
• Trypsinimpairmentpreventsproteinslikegluteninwheatfrombeingdigested
• Undigestedproteinsinducereleaseofzonulinwhichopensupgutbarrier**
• ZonulinlingersbecausetrypsinisdefecSve
*ASamselandSSeneff.JBiolPhysChem2017;17:8-32**JJGildeaetal.JClinNutrDiet.2017,3:1.
AScenario
ZonulinTrypsindefecSve
Zonulinreleased
LeakybarrierGliadin
Leakybrainbarrier
Glyphosateinwheat
Autoimmunedisease
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Whatifglyphosatecouldinsertitselfintoproteinsynthesisbymistake???
GGC
mRNA
glyphosate
Alanine ProlineGlyphosate
Anyproteinswithconservedglycineresiduesarelikelytobeaffectedinamajorway
......
Codeforglycine
Whatifglyphosatecouldinsertitselfintoproteinsynthesisbymistake???
GGC
mRNA
glyphosate
Alanine ProlineGlyphosate
Anyproteinswithconservedglycineresiduesarelikelytobeaffectedinamajorway
......
Codeforglycine
Trypsincontainsseveralglycine-richregionsthatareessenSalforitsfuncSonasadigesSveenzyme
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H
GlycineGlyphosate
Glyphosateisanon-codingaminoacidanalogueofglycine
ExtraPieceS7cksOutatAc7veSite
Alanine ProlineGlyphosate
glycine
AcSveSite
ExtraStuff
PepSdechain
SubstratenolongerfitsinacSvesite
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ExtraPieceS7cksOutatAc7veSite
Alanine ProlineGlyphosate
glycine
AcSveSite
ExtraStuff
PepSdechain
SubstratenolongerfitsinacSvesite
Thisexplainshowglyphosatedisrupts
EPSPSintheshikimatepathway:MulSplebacteriahavedevelopedresistancebyreplacingacSvesiteglycinewithalanineandthisisthe
basisforGMORoundupReadycrops*
*TFunkeetal.,MolecularbasisfortheherbicideresistanceofRoundupReadycrops.PNAS2006;103(35):13010-13015.
Inhibi7onofEPSPSbyglyphosate:ResistantEcolimutant*
*Figure3,SEschenburgetal.Planta2002;216:129-135.
Wildtypewithglycine96
MutantwithalaninesubsStutedforglycine96
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GlyphosateinVaccines?• ForMMR,fluvaccine,andrabiesvaccine,livevirusisgrownongela'nderivedfromligamentsofpigsandfetalbovineserum– CowsandpigsarefedGMORoundup-Readycornandsoyfeed
• GelaSnisderivedfromcollagenwhichishighlyenrichedinglycineandalsocontainsglutamate– ThesetwoneurotransmiOersexcitetheNMDAreceptorsinthebrain
• GlyphosatesSmulaSonofNMDAreceptorscouldcauseneuronalburnout
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GlyphosateExcitesNMDAReceptorsinHippocampus*,**
*DCaOanietal.Toxicology2014;320:34-45.**DCaOanietal.Toxicology2017[Epubaheadofprint]
Excessglutamateinsynapseleadstoneuronalburnout
GlyphosateExcitesNMDAReceptorsinHippocampus*,**
*DCaOanietal.Toxicology2014;320:34-45.**DCaOanietal.Toxicology2017[Epubaheadofprint]
Excessglutamateinsynapseleadstoneuronalburnout
Ratsexposedtoglyphosateperinatallyexhibiteddepressivebehaviors
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GlyphosateExcitesNMDAReceptorsinHippocampus*,**
*ZZhengetal.PLOSONE2016;11(7):e0158688.
Excessglutamateinsynapseleadstoneuronalburnout
Glutamateiselevatedinthebloodin
associaSonwithauSsm,andbloodglutamatelevelscorrelatewithbrainglutamatelevels.*
Au7sm,Glyphosate,VaccineReac7ons*
*DatareadilyavailablefromtheCDC,FDA(VAERS)andUSDA
GlyphosateapplicaSontocornandsoy,US
#AdverseReacSonsinVAERS
ChildrenwithAuSsm,US
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*ASamselandSSeneff,JournalofBiologicalPhysicsandChemistry2017;17:8-32.
*ASamselandSSeneff,JournalofBiologicalPhysicsandChemistry2017;17:8-32.
…Mostdisturbingisthepresenceofglyphosateinmanypopularvaccinesincludingthe
measles,mumpsandrubella(MMR)vaccine,whichwehaveverifiedhereforthefirstSme.
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GlyphosateContamina7oninVaccines(PartsPerBillion)*
Merck ZOSTAVAX 0.62ShinglesMerck MMR-II 3.74Measles,Mumps andRubella
Merck VARIVAX 0.56 Varicella,ChickenPoxMERCK PNEUMOVAX ND Pneumococcal18MERCK PROQUAD 0.66Measles,Mumps, Rubella,Varicella
GSK ENERGIX-B 0.34HeptaSSsB
*ASamselandSSeneff,JournalofBiologicalPhysicsandChemistry2017;17:8-32.
SymptomsofAdverseReac7onstoMMRbeforeandaVer2002*
DataanalyzedfromtheVAERSdatabase*ASamselandSSeneff.JBiolPhysChem2017;17:8-32
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SymptomsofAdverseReac7onstoMMRbeforeandaVer2002*
*DataanalyzedfromtheVAERSdatabase
TheseareallcharacterisScsymptomsofallergiestoMSG
seizures
hives
swellingswelling
Jointpain
FacialswellinganaphylacScshock
shortnessofbreath
eczema
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“NobelLaureateCharlesRichetdemonstratedoverahundredyearsagothatinjecSngaproteinintoanimalsorhumanscausesimmunesystemsensiSzaSontothatprotein.SubsequentexposuretotheproteincanresultinallergicreacSonsoranaphylaxis.Thisfacthassincebeendemonstratedoverandoveragaininhumansandanimalmodels.”
LargeProteinsinVaccines:Allergenic*
“VaccinesclogourlymphaScsystemandlymphnodeswithlargeproteinmoleculeswhichhavenotbeenadequatelybrokendownbyourdigesSveprocesses,sincevaccinesbypassdiges'onwithinjecSons.Thisiswhyvaccinesarelinkedtoallergies,becausetheycontainlargeproteinswhichascirculaSngimmunecomplexes(CICs)or'klinkers’causeourbodytobecomeallergic.”
*DaveMihalovic,ND,hOp://whale.to/v/vaccines_cause_allergies.html
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ThePurdueVaccina7onStudiesandAutoan7bodies*
“ThePurduestudies… foundthatvaccinateddogsweredevelopingautoanSbodiestotheirowncollagen”
*hOp://www.dogsnaturallymagazine.com/purdue-vaccinaSon-studies/
AScenario
2.Livemeaslesvirusinfectsgut
3.MeasleshaemaggluSninundigested
4.Inducesleakygut
6.Zonulininducesleakybrainbarrier
6.Measlesvirusinfectsbrain
7.Brain’simmunesystemproducesanSbodies
8.AutoanSbodiesaOackmyelinsheath
1.ChildisvaccinatedwithMMR
5.Measlesvirus
5.Zonulin
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MeaslesVirusandHaemagglu7nin*• ThemeaslesvirussynthesizestheproteinhaemaggluSnin
– AnSbodiestohaemaggluSninareessenSalfollowingMMRvaccinaSontoinduceimmunity
• Measlesvirusinfectsbrainduetoleakybarrier• HaemaggluSnbearsasequenceresemblancetomyelinbasic
protein(MBP)àpotenSalforautoimmunereacSon• MBPisessenSalfortheformaSonofthemyelinsheath
surroundingnervefibers• AutoanSbodiestoMBPalongwithexcessivelevelsof
anSbodiestomeasleshaemaggluSninarelinkedtoauSsm**
*Oldstone,MBA,Ed.Molecularmimicry:InfecSoninducingautoimmunedisease.SpringerBerlinHeidelberg;January9,2006.**VKSinghetal.,JBiomedSci2002;9(4):359-64.
Au7smandMeaslesHaemagglu7nin*
• 125auSsScchildrenand92controlchildren• 60%ofthechildrenwithauSsmhadhighlevelsofanSbodiestomeasleshaemaggluSninspecifictotheMMRvaccine– 90%ofthesehadautoanSbodiestomyelinbasicprotein(MBP)
• 0%ofthecontrolchildrenhadhighanSbodySterstoeitherhaemaggluSninorMBP
• TherewerenoelevaSonsinanSbodiesdetectedagainstanyproteinsinthemumpsorrubellaviruses
*VKSinghetal.,JBiomedSci2002;9(4):359-64.
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Summary• Glyphosate’sinsidiouscumulaSvetoxicitymaybeduetoits
abilitytogetintoproteinsbymistakeinplaceofglycine• GlyphosatehasbeendetectedinmulSplevaccines,with
highestconcentraSonsinMMR– ManyvaccinesincludegelaSnandfetalbovineserumasingredients,
bothofwhicharecontaminatedwithglyphosate
• Glyphosateandglutamate(fromthevaccine)canexciteNMDAreceptors,andcauseneuronalburnout
• GlyphosatecontaminaSonleadstoresistancetobreakdownandautoimmunediseasethroughmolecularmimicry– AcausallinkbetweenMMRandauSsmcanbeexplainedthrough
autoimmuneaOackonmyelinbasicproteinviamolecularmimicrywithmeasleshaemaggluSnin
• MulSplelargeproteinsfoundinvaccinesareallergenicandtheymaybeacausalfactorinfoodallergiesandanaphylaxis