Anti-ageing Stem Cells Therapy - Golden Vial for Rejuvenation

PROFESSOR OLEKSANDR KUKHARCHUK

21 April, 2015

What is aging? Theory of aging before the discovery of stem cells was more than 300. All seems to be erroneous. Scientific descriptions of mechanism of aging were similar to history of the blind and the elephant… One day an elephant was brought to a city. Six blind people decided to know – what is elephant? – And they continued to study. Four groped the feet of elephant, one – trunk, and the last one – tail. Later they said: “Elephant – it is four big thick trees”, - said those who groped the legs. “No! This is big thick snake”, - said by who groped the trunk. “No”, - said the one who groped the tail, “Elephant – it is a small thin lizard”.

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Today we exactly know that aging is related due to decrease of stem cells in the organism of humans. Cells of our body constantly renews, for example:Skin – 3 days; Stomach – 5 days;Intestine – 7 days.How this renewal does take place? Very simple: stem cells divide and give rise to new cells in the organism. In all adult tissues and organs contains stem cells without any exception. The division of cells provides renewal of organ and tissue which gives their youth and youthfulness of the whole organism of body. But with age, number of stem cells progressively decreases. Cells of the tissues stop to renew and ages. And together with cells human also ages.So: Aging – it is decrease of stem cells reserve in organs and tissues of the human.CONCLUSION: For true rejuvenation need to increase stem cells reserve in human organs and tissues.

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What are aging diseases? Experienced doctors say that until now no one has died of aging. All die of diseases. What are these diseases that people die in aging? Cancer, myocardial infarction, stroke, diabetes mellitus, Alzheimer’s disease, Parkinson, osteoporosis, arthrosclerosis, hypertension, immunodeficiency – this is not complete list that are named as DISEASE OF AGING.Why particularly these diseases kill us in aging? Answer is very simple – they are caused by the same reason of aging: due to decrease of stem cells reserve in the tissues of human organism.

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Well known fact that with age the immune system weakens. This is related with that cells of the immune system does not renew. They age and lose their power. Such weak cells already cannot destroy the constantly arising cancer cells and develops carcinoma in humans. In the early stages 40-50 of yrs aging the renewal of immune system cells is already disturbed. As the aging changes to immune cells appear, they start to attack and destroy their own cells of organs and tissues instead of protecting the organism. In this manner autoimmune disease develops – diabetes mellitus, liver cirrhosis, glomerulonephritis, arthrosclerosis, and multiple sclerosis. Deep aging of immune system leads to development of immune-deficiencies and aging humans die of infectious diseases – such as inflammation of lungs, sepsis, meningitis.

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Cells of vascular innermost membrane (intima) – endotheliocytes renew rapidly – once in 7 days. If reserve of stem cells forming new endotheliocytes reduces with age then the renewal of vascular innermost membrane (intima) does not occur. As they age covers with atherosclerotic plague blood flow through vessels gets disturbed and people die of stroke, myocardial infarction, thrombosis of pulmonary artery.Therefore: reason of aging diseases is as aging itself the decreases of stem cells reserve in organs and human tissues.CONCLUSION: to block the development of aging disease it is necessary to increase reserve of stem cells in organs and human tissue.

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What is chronological age and biological age?Can we know how much stem cells reserve remains in our organism? In other words can we know how faster are we aging and when will diseases of aging arise? Direct method of calculating number of stem cells in the organism of living human does not exist. But there are scientific evidential based methods of evaluating intensiveness of human aging. One among them is the method comparison between chronological age and biological age of humans.Chronological aging – it is number of year from the moment of your birth till date of your life. This is your life. This – “Age according to date of birth”.Biological age – this measures the change over time of the biological possibility of organism and its back up ability to the vital systems. It allows the prognosis of human life expectancy. This is “level of age wear” your body depends on stem cells reserve.

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Example. Research of Zagumenova O. (Russia, 2000), showed that in men and women (age 25-50 years, altogether 398 people), who lived for ten years in the area of radioactive contaminated territory after Chernobyl nuclear station incident the biological age was higher than that of chronological by 9.2±0.5 years. This indicates that people in radiation zone aged to 10 years older and 79% of people were identified with aging diseases. Pay attention! Radiation kills active stem cells! Intensity of aging sharply increases and radiation made people premature aging to 10 years older due to the decrease in stem cells reserve.

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Objective criteria of anti-aging therapy efficacy evaluatingAging in each person is individual and characterized by qualitative point of view – type of aging, by quantitative point of view – rate of aging, with the structure of aging point of view – ratio of different system of aging rate i.e. profile of aging.Differentiated 4 types of aging: cardio vascular, endocrine, neuro-psychological, metabolic, and miscellaneous.Profile aging determined in unevenly aging by ratio of aging rate of different organ and system. Profile aging determined by cardiovascular system, respiratory, muscular, nervous, immune system, vision, hearing, and biochemical parameters of blood.

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Standard quantitative characteristics of aging are ratio of biological and chronological age. Methods of determining biological age are many – from simple to very complicated, which requires doctors consultation and expensive analysis. Fortunately currently developed and successfully tested method of complex evaluation of biological age, which could be used individually even without our participation. Program of determination of biological age you could find internet through links: http://xn--b1adef0ban2h.com.ua/sam-sebe-sanolog/biologicheskij-vozrasthttp://faberlic-voronezh.ru/tests/bioage/?test=1

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This would take not much of time. As a result you could compare chronological age with biological age. This could help you to take decision on rejuvenation (anti-aging). For example if your chronological age is 35 years, and test shows your current biological age is 45 then you have problem this is fast aging which needed to fight. Otherwise decrepit appearance and complex old age diseases are awaited ahead. At the same time any scale in which biological age soar up over chronological age already requires rejuvenation even if this difference is 1 year.Can we rejoice if chronological age and biological age coincides? Alas, no. This indicates only about that your body is getting older in time. Aging, but is not rejuvenating. But ideally should be on the contrary – your biological age should be lesser than the chronological!

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If biological age is more than the chronological, this indicates that period of functional system aging changes accumulation beginning. Objective characteristics of aging can be converted into new quantitative and qualitative level – the level evaluating index of age damage and deficit of functions.This index calculated by formula:The frailty index is calculated as:The number of deficits that an individual hasThe total number of deficits considered10 deficits presents in an individual = Frailty index score of 10/40=0.2540 deficits considered in total Measuring frailty as an index of (40) deficits The higher the Frailty Index, the more frail the individual

List of 40 deficit functions needed to be calculated for age damage index, mentioned in the below table:

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Deficit Level Deficit Level Deficit Level Deficit Level Deficit Level

Eyesight 5 Ability to take care of appearance

3 Help to take a bath or shower

3 Help to prepare own meals

3 Self-rated health

3

Hearing 5 Help to walk 3 Help to use the telephone

3 Help to do housework

3 Troubles prevent normal activities

3

Help to eat 3 Help to get in and out of bed

3 Help to travel beyond walking distance

3 Ability to take medications

3 Lives alone 2

Help to dress 3 Help to go to the bathroom

3 Help with shopping

3 Ability to handle own money

3 Having cough 2

Deficit Level Deficit Level Deficit Level Deficit Level Deficit Level

Feeling tired 2 Stroke or effects of stroke

2 Ear trouble 2 Kidney trouble 2 Trouble with feet or ankle

2

Nose stuffed up or sneezing

2 Arthritis or rheumatism

2 Dental problems 2 Losing control of bladder

2 Skin problems 2

High blood pressure

2 Parkinson’s disease 2 Chest problems 2 Losing control of bowels

2 Fractures 2

Heart and circulation problems

2 Eye trouble 2 Trouble with stomach

2 Diabetes 2 Trouble with nerve

2

List of 40 deficit functions needed to be calculated for age damage index, mentioned in the below table:

Wide-zoom (experimented around 70000 people) study of Rockwood et al. (2011) revealed precise exponential relation of Frailty Index to age:

00.05

0.10.15

0.20.25

0.30.35

0.4

10 20 30 40 50 60 70 80 90 100

Frai

lty In

dex

Age (years)

National Population Health Survey – Mean Frailty Index at each cycle in relation to age (Rockwood et al., CMAJ 2011; E-pub April 28)

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This study proved that Frailty Index is objectively quantitative and qualitative criteria for evaluating aging.In experimental study of Parks et al. (2011) was proved that Frailty Index process of aging increases both in men and women – aging does not spare anyone.

Adult Aged

Males 0.08 0.5Females 0.09 0.39

00.10.20.30.40.50.6

Frai

lty In

dex

Frailty Index in Males and Females

Parks et al., J Gerontol Biol Sci, 2011

Experimental study Shi et al. (2011) showed that as the Frailty Index increases probability of death sharply rises. Thus more higher the Frailty Index lesser the life span.

0

0.2

0.4

0.6

0.8

1

1.2

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7

Dea

th R

ate

Frailty Index

Men

Women

For men & women, deficit accumulation is highly related with mortality (r>0.95); men have a higher death rate than women (Shi et al., BMC Geriatr. 2011 Apr 20; 11:17)

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Conclusion: Currently are two objective parameters which characterizes aging. First – it is difference between chronological and biological age, second – Frailty Index.How not only to appear younger but actually to become younger?How vital physiological systems ages?Nervous system. Skin sensitivity, vision and hearing acuity and blood supply to tissue reduces.Endocrine system. Male and female hormone production (testosterone, estradiol), and insulin reduces. In period where in men and women climax changes in all level of endocrine system:Immune system. Reduces all types of immune protection of the body: non-specific reactivity, cellular and humoral immunity, anti-cancer and anti-fungal immunity. Autoimmune aggression is developed. Cardio-vascular system. Inner membrane of artery thickens, muscle layers get atrophied, reduces vascular wall elasticity, vein broadens, tone of heart walls reduces, cardiac muscle power reducesRespiratory system. Mucosa of trachea and bronchi gets atrophied, lungs elasticity reduces, vital capacity decreases. Atrophic and fibrotic-dystrophy process of respiratory muscles commences.

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Digestive system. Salivary gland, mucous and muscular membrane of the esophagus, stomach and intestines atrophies, and secretary function reduces. Cells of pancreatic gland starts to atrophy, number of beta-cells reduces which produces insulin. Production of pancreatic enzymes reduces – trypsin, amylase and lipase. Mass of liver and its size reduces. Lipids accumulates in hepatocytes, bile flow reduces. Fibrotic musculature changes at the bottom of gall bladder and reduction of wall elasticity leads to increase of residual volume of bile which is capable of stone formation.Urinary system. Renal vessels gets sclerosis, renal blood supply reduces. After 70 yrs number of functioning nephrons reduces to 50%, glomerular filtration rate reduces. Urinary bladder wall losses its elasticity and thickens. Atrophy occurs in the muscular layer, contractibility feature reduces, and sphincter weakens.Muscle-skeletal system. Most characteristics to reduce thickness of bone tissue – osteoporosis is the reason of bone fracture arising in the senile group of people mostly in vertebrae, radius and femoral neck. Muscle power reduces; they become lethargic, hypo-tropic.THESE CHANGES OCCURS IN ALL INDIVIDUALS WITHOUT ANY EXEPTIONAL CASES.

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What leads the aging of vital physiological systems to? Aging of physiological system creates a base for series of disease development. Below we mention most common out of those: arthrosclerosis and arterial hypertension, chronic ischemic heart disease, myocardial infarction, heart failure, stroke, cancer, autoimmune disease, pneumonia and respiratory failure, stomach ulcers and duodenal bleeding, perforation, penetration and malignant diseases, blindness, deafness, fracture, osteomalacia, joint diseases, renal disease, liver, diabetes mellitus, asthenia, depression, senile dementia, Alzheimer’s disease.Why vital physiological system ages? Say for instance that 100% of cells of a single organ works in young healthy body whereas with those number of cells people live till the senile equals to 25-30% and remaining 70-75% dies or losses its function as the result of wear and diseases. However well known that after removal of 50% of cells from vital organs (lungs, liver, kidney, and adrenal) in the younger age the individual can still live for long. Fact is that young stem cells enough for regeneration of damaged organs. In the process of aging stem cells reserve sharply reduces which leads to wear of physiological systems and diseases of aging. Subsequently for true rejuvenation requires increasing stem cells reserve in the aging body.So, important indicator of actual rejuvenation is period of healthy life. Successful aging – this is absence of disease and disability, saving capability to assimilation of new information and physical activity in family and society. That is successful aging – this is active longevity.

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Program and aim of rejuvenation with stem cells Any treatment program has its own goals. Goals of our treatment: 1. To eliminate aging changes of vital physiological system and prevent them from aging in near 10 years after stem cells transplantation.2. To eliminate or to stop aging disease development – to provide patient successful aging.3. To normalize ratio of biological and chronological age for patients elder than 60 yrs and reduce the chronological age in comparison with biological age for the patients who are younger than 60 yrs.4. To restore function of immune system in the level of young organism 5. To provide patient the external rejuvenation with the help of bio-cosmetic procedure for hair, face, neck, décolleté, and hand.Program includes transplantation of fetal stem cells “GOLDEN VIAL” and special complex bio-creams which provides regeneration of hair, skin and nails.

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What is “GOLDEN VIAL”? To restore all functional system and rejuvenation of the patient we need to increase of organ and tissue specific stem cells reserve in the whole body: In brain, heart, lungs, vessels, liver, kidneys, bone marrow, tissue of immune system and endocrine system, organs of vision and hearing, skin, muscles and bone tissue – altogether required to renew more than 240 different types of stem cells. This can be done exclusively with the help of fetal progenitor cells transplantation. Golden vial – it is program of transplantation of all types stem cell (without any exception!) which is necessary for complete renewal of all functional system and rejuvenation.About our complex biological active creamsUnique outstanding composition of our creams contains extracts of stem cells specific for hair follicles, face, neck and hand. Symphony from these musical notes of our cream is to awaken your dormant youth reserve which returns your delight in socialization, build in confidence, blossom and beauty!

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Creams composition: fetal growth factors of skin and hair, cytokeratins K17, involucrin, dermal Ki-67, fibronectin and chondroitin sulfate, collagen type III, I, glycosaminoglycans, SKALP (skin derived antileuko proteinase), triglycerides, sterol ester, glycosphingolipids, hyaluronan, hyaluronan stimulating activity.Properties of creams:Stimulation of own stem cells of the skin and hair follicle of the patient Whitening - inhibits the melanin which gives age spots and dullness to skinAnti-inflammatory effect - repress pimples inflammation, rednessMoisture - restores normal moisture retaining properties of skinCell proliferation - improves metabolism, and generates healthy skin.Collagen (fine, reticular, less cross-linking) production - improves collagen generation which signifies skin elasticity. Blood circulation – improves blood circulation Amino acids – building blocks of the cells Antioxidant effect – restrains active enzymes that cause aging

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1. Anti-aging Face Cream 50 ml (for 1 course). The Cream is absorbed intensively into the dermis to strengthen the revitalize collagen and elastin for moisture retention. Tissue extracts of skin can regulate and normalize cases of pigmentations such as melasma and age spots, pigmentation, acne, and scar, induce skin healing, and help overcome other skin problems. Skin looks plump with increased hydration and radiant luminosity. Renewed sense of skin firmness is felt.2. Anti-aging Neck Cream 50 ml (for 1 course). Cream gives younger, firmer, smoother skin and reduces rough and crepey skin. The cream has powerful effect of lifting, moisturizes and refines skin of neck and the area of decollete, eliminates undesired lines, rejuvenates skin due to the stimulation of own stem cells of the skin.3. Anti-aging Hand Cream 50 ml (for 1 course). Moisturizes and refines skin of hands, eliminates undesired lines, rejuvenates skin of hands due to the stimulation of own stem cells of the skin. Rich, protective hand cream to deeply moisturize and restore smoothness, Leaves hands feeling soft and supple. Provides long-lasting hydration and natural antioxidant protection.

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4. Anti-aging Cream for Neck and Jaw Line 50 ml (for 1 course). Intensified lifting of skin (night). Reduces the sagging jaw by firming the skin texture, helps in obtaining pronounced jaw-line.5. Hair Cream 100 ml (for 1 course). Hair follicle regeneration is characterized by dramatic changes in its microanatomy and cellular activity. During hair follicle active growth and hair production, the activity of factors promoting proliferation, differentiation, and survival predominates, while hair follicle regression is characterized by activation of various signaling pathways that induce apoptosis (programmed cell death) in hair follicle cells. Instant stop of hair fall stimulates growth of new hair due to activation at the level of scalp hair follicles by specific fetal hair follicle growth factors.Results of our patients’

To prevent disease and delay commencement of aging Program of rejuvenation “Golden Vial” successfully approved in Ukraine and Russia. With the help of “Golden Vial” many famous people received rejuvenation. Below we have shown average statistics data of rejuvenation results in 365 people (247 female and 138 male) and likewise quoting evaluation of patients who has completed program of “Golden Vial”.

General Characteristics of patients, participated in study (before treatment)Age group

Возрастные группы

Female, n = 247 Male, n = 138

CA,Yrs

BAYrs

CA – BA ± Yrs

FIUnits

CA,Yrs

BAYrs

CA – BA ± Yrs

FIUnits

30-39 yrs

34.8±1.5n = 56

40.3±1.7n = 56

+5.5±0.8n = 56

0.33±0.02n = 56

35.4±1.6n = 22

41.4±1.8n = 22

+6.0±0.6n = 22

0.35±0.03n = 22

40-49 yrs 45.3±1.9n = 103

52.6±2.0n = 103

+7.3±0.5n = 103

0.38±0.03n = 103

46.1±1.5n = 75

53.6±1.7n = 75

+7.5±0.4n = 75

0.41±0.02n = 75

50-59 yrs56.5±2.1

n = 6566.0±2.4n = 65

+9.5±0.7n = 65

0.42±0.03n = 65

53.8±2.3n = 24

63.6±2.9n = 24

+9.8±0.5n = 24

0.47±0.04n = 24

60-69 yrs63.3±2.5

n = 2374.1±2.7n = 23

+10.8±0.9n = 23

0.47±0.04n = 23

64.2±2.7n = 23

75.6±3.1n = 23

+11.4±0.8n = 23

0.52±0.04n = 23

70-80 yrs72.2±3.0

n = 983.5±3.2

n = 9+11.3±1.2

n = 90.53±0.04

n = 971.9±3.3

n = 783.9±3.5

n = 7+12.0±0.9

n = 70.58±0.05

n = 7

CA – Chronological age. BA – biological age. CA – BA – difference between biological and chronological age. FI - Frailty Index

Frequency of aging symptoms among all study patients of all age group (%)

Symptoms F M Symptoms F M

Presence of grey hairs 44 47 Lip chapping 24 25

Presence of baldness, less dense hair growth on scalp

52 59 Frequent (2-3 times/year) herpes on mouth

48 39

Hair fall 26 5 Symptom "worm" – a throbbing, thickening of temporal artery

41 78

Symptom of diagonal folds of ear 95 98 Symptom "red drops" – hemangiomas on the body, telangiectasia, spider veins

80 97

Wrinkles on the face and neck 50 57 Symptoms of leukolonychia (white spots on the nails)

55 46

Xanthelasmatosis 8 29 Face asymmetric 5 10

Symptom of rabbit eye (sclera injection) 6 30 Dryness of skin, edema and paleness of skin

11 17

Pigment dots and appearance of age senile nevus

37 51 Symptom "dark", scaly hands and knees 15 21

Scanty hairs in eyebrows and eyelashes 75 88 Hyper-pigmentation of orbit skin, palmar creases

2 9

Imprints of teeth in the tongue 3 15 Impaired smooth speech 14 7

Coating on tongue, “geographic tongue” 9 13 Hyperemia of palms and soles 36 47

Frequency of aging diseases among all study patients of all age group (%)

Aging Disesaes F M Aging Disesaes F M

Atherosclerosis 53 79 Immunodeficiency 69 53

Arterial hypertension 32 66 Osteoporosis 78 24

Arterial hypotonic 57 5 Joint diseases 68 36

Ischemic cardiac disease 5 13 Diabetes Mellitus 9 6

Heart failure 3 7 Weakness of hearing and vision 61 67

Post stroke condition 4 2 Asthenia syndrome 71 19

Autoimmune disease 45 44 Depression 46 12

Characteristics of morphological type of aging of the face in study groups of female

Type of aging Signs Number of observation

%

Fatigue morpho-type

Reduction of tone in the smooth tissues of the face and muscles, dry and dull skin. Early appearance of folds and wrinkles, deepening of naso-labial folds, lowering of mouth corners and appearance of wrinkles there (wrinkles “puppets”), lacrimal groove.

157 63.6

Wrinkles morpho-type

Wrinkles due to thinning of skin, dry, prone to irritation and redness of skin. Poor contours saggy smooth tissue of face.

59 23.9

Deformative morpho-type

Puffiness of face tissue, flabby pore skin. Redness of cheek with deformities face contours, second chin formation, “flew”, neck folds, eye bags and droopy eyelids.

28 11.3

Muscular morpho-type

Disturbance in pigmentation, folding of upper and lower eyelids, pronounced naso-labial folds. Skin of cheek is flat and smooth. Contours of oval face save up to old age.

3 1.2

Gerontology and clinical-instrumental analysis was performed after 6, 12, 18, 24, 36, 42, 48, 54 and 60 months after patients undergoing of full program of “Golden Vial”: Evaluation of viscoelastic parameter of hand skin method of cutometer (Cutometer, Courage & Kazaka, Germany). Method based on the principle of vertical deformation. Parameter: F – maximum level of skin deformation; U – elasticity of skin.

020406080

100

0 6 12 18 24 30 36 42 48 54 60

units

months

F

U

Dynamics of patient’s cutometer parameter after undergoing complete program of “Golden Vial”

Result: Maximum level of the skin deformation reduced parallel to increase its elasticity in whole observation period.

Clinical evaluation conducted by the following features: roughness, radiance and uniformity of hand skin tone (by scale in points).

0

2

4

6

8

10

0 6 12 18 24 30 36 42 48 54 60

units

months

Dynamics of parameters of the smoothness (green line), radiance (brown line) and uniformity of hand skin tone (blue line) after patients undergoing complete

program “Golden Vial”

Evaluation of results. Evaluation results of skin rejuvenation of hand, neck, décolleté and face of doctors and patients conducted based on the fundamental international evaluation scale GAIS (Global Aesthetic Improvement Scale):

Points Evaluation of doctors Evaluation of patients

3 Optimal cosmetic results for the given patient

Complete satisfaction of results

2 Significant improvement, but not complete correction

Satisfied of the results, but wanted to improve it little more

1 Improvement, but required to additional correction

Improvement insignificant, desirable to additional correction

0 No changes, condition similar as before procedure

No changes

−1 Deterioration in comparison with the initial condition

Condition is worse than before performing procedure

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Evaluation of doctors:Optimal cosmetic results for the given patient - 198 (80.2%)Significant improvement but not complete correction – 21 (8.5%)Improvement, but required additional correction – 28 (11.3% – practically all women with deformities type of face of aging) No changes, condition same as before procedure – 0 Deterioration in comparison to initial condition – 0 Evaluation of patients:Completely satisfied of results – 195 (78.9%)Satisfied of the results, but wanted to improve it little more – 24 (9.7%)Improvement insignificant, desirable for additional correction – 28 (11.3% – practically all women with deformities type of face of aging)No changes – 0Condition worse, than before performing procedure – 0

Dynamics of Frailty Index in female and male age group of 30-39 yrs after patients undergoing complete program “Golden Vial”

Dynamics of Frailty Index in female and male age group of 40-49 yrs after patients undergoing complete program “Golden Vial”

Dynamics of Frailty Index in female and male age group of 50-59 yrs after patients undergoing complete program “Golden Vial”

Dynamics of Frailty Index in female and male age group of 60-69 yrs after patients undergoing complete program “Golden Vial”

Dynamics of Frailty Index in female and male age group of 70-80 yrs after patients undergoing complete program “Golden Vial”

Dynamics of Frailty Index in female of all age group after patients undergoing complete program “Golden Vial”

0

0.1

0.2

0.3

0.4

0.5

0.6

0 6 12 18 24 30 36 42 48 54 60

Frai

lty In

dex,

uni

ts

months

30-39 yrs

40-49 yrs

50-59 yrs

60-69 yrs

70-80 yrs

Dynamics of Frailty Index in male of all age group after patients undergoing complete program “Golden Vial”

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0 6 12 18 24 30 36 42 48 54 60

Frai

lty In

dex,

uni

ts

months

30-39 yrs

40-49 yrs

50-59 yrs

60-69 yrs

70-80 yrs

.

Conclusion: better results observed in patients of age 30-39 yrs. Consequently earlier the rejuvenation program started “Golden Vial”, lesser the probability of aging disease development. But even in patients of age groups 70-80 yrs our program significantly reduces Frailty index that evidences about the decrease in risk of aging diseases appearance.

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Dynamics of difference between biological and chronological age parameters in female and male age group of 30-39 yrs after patients

undergoing complete program “Golden Vial”

Dynamics of difference between biological and chronological age parameters in female and male age group of 40-49 yrs after patients

undergoing complete program “Golden Vial”

.

Dynamics of difference between biological and chronological age parameters in female and male age group of 50-59 yrs after patients

undergoing complete program “Golden Vial”

Dynamics of difference between biological and chronological age parameters in female and male age group of 60-69 yrs after patients

undergoing complete program “Golden Vial”

.

Dynamics of difference between biological and chronological age parameters in female and male age group of 70-80 yrs after patients

undergoing complete program “Golden Vial”

Dynamics of difference between biological and chronological age parameters in female of all age group after patients undergoing

complete program “Golden Vial”

-20

-15

-10

-5

0

5

10

15

0 6 12 18 24 30 36 42 48 54 60

±yr

s

months

30-39 yrs

40-49 yrs

50-59 yrs

60-69 yrs

70-80 yrs

Dynamics of difference between biological and chronological age parameters in male of all age group after patients undergoing

complete program “Golden Vial”

-20

-15

-10

-5

0

5

10

15

0 6 12 18 24 30 36 42 48 54 60

±yr

s

months

30-39 yrs

40-49 yrs

50-59 yrs

60-69 yrs

70-80 yrs

.

Conclusion: Dynamic changes of difference between biological and chronological age evidences that with the help of rejuvenation program “Golden Vial” true rejuvenation is reached. Maximum result in the age group for female:30-39 yrs – to 15.6 yrs40-49 yrs – to 15.8 yrs50-59 yrs – to 13.6 yrs60-69 yrs – to 10.8 yrs70-80 yrs – to 4.9 yrs Maximum result in the age group for male:30-39 yrs – to 16.4 yrs40-49 yrs – to 16.5 yrs50-59 yrs – to 12.6 yrs60-69 yrs – to 11.7 yrs70-80 yrs – to 5.4 yrs Thus, observing these above data you could anticipate your rejuvenation after undergoing complete rejuvenation program “Golden Vial”.

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Case History (Female)Female, 37 yrs, actress of theatre and movie. “Approached us and said I am scared of aging. I have problem with face, neck, hand, and breast skin. Hair is like oakum, falling – morning whole comb “hairy”. Nails are simply terrible, hands have some small pale brown pigmentation, wrinkles in fine mesh, often appearance of chapping. Off late cannot read anything without spectacles, cannot hear the interlocutor while talking in mobile phone but better in the left than the right ear therefore I hold it away. Volatile pain in the small joints of hands and leg, by the mid day I have “crumpled face”, and most important I noticed that my cream and other cosmetic products does not help. In shooting I become tired easily and even could break out with tantrum. Friends tell that I have become easily resentful, tearful, irritable and rude. I have frequent ailments and cold, menstrual cycle is disturbed. I am sleepless at nights and sleepy in the noon. I have reduced libido and scandals with husband. Shout at children, dry skin and when I panic and worry become sweaty and tremor of hands. Sweat smells very unpleasant. Feel often tiredness. Dress does not fit already; I need to change the wardrobe. In the mirror to see myself very scary: wrinkles, cellulites, simply terrible. I don’t want to see anyone, don’t to meet school friends, birthdays, marriages, parties, chat with friends. I have often depression, I started to smoke, consume alcohol often to remove stress and aging. I am just 37 please help me!”

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Result of complete medical analysis of patients. Biological age exceeds chronological age on 9 years. Disturbances were identified in cardiovascular system (arterial hypotonic), joints (polyarthritis), immune system (T-cell deficit) and endocrine (unbalanced production of estrogen and progesterone). Frailty Index – 0.375.Gerontological diagnosis: Early (accelerated) aging [+9 yrs – 37 yrs wear of functional system of patient corresponds to 46 yrs, early pathological climacteric status], type of combined aging (cardio-vascular, endocrine and neuro-psycho-somatic).Treatment: complete course “Golden VialTreatment Results:

2005 yr 2006yr 2007 yr 2008 yr 2009 yr 2010 yrBA>CA= + 7yrs Frailty Index = 0.375Undergone complete program of “Golden Vial”

BA>CA= + 2yrs Frailty Index = 0.275Improvement of condition of functional system of menstrual cycle-normal

BA<CA= − 1yr Frailty Index =0.050Functional systems - normal External appearance – normal for the current age menstrual cycle – normal

BA<CA= − 3yr Frailty Index =0.025Functional systems - normal External appearance - rejuvenation menstrual cycle – normal

BA<CA= − 5yr Frailty Index =0.025Functional systems - normal External appearance - rejuvenation menstrual cycle – normal

BA<CA= − 5yr Frailty Index =0.025Functional systems - normal External appearance - rejuvenation menstrual cycle – normal

Case History (Male)Male, 42 yrs, successful business men. “One day I woke up and I noticed in the mirror my moustache and temple region got grey hairs. Took second small mirror and found that in the region of crown scanty hair and began to take shape of bald. I changed my hair style, started to comb from forehead to back so that to hide thinning of hair. Started to analyze whole health condition and surprised of aging signs. First of all, potency and interest in women indifference to sex has reduced. I became irritable. Surprised unpleasantly that redness of face appears during pressurized work after meeting or important conversations. It warned me that, by the end of the day face remained red, irritable, feeling of unreasoning anxiety and even fear. Unpleasant odor in mouth from the morning appears to be even though last evening didn’t drink alcohol. Started to get tired faster at work and in sports hall, muscle strength has gone down. I decreased interest to work and success. Weakness and apathy period has started. That why I have approached your help”.Result of complete analysis of patient. Biological age exceed the chronological age by 13 years. Disturbances were identified in cardiovascular system (arterial hypertension), liver (reduced detoxification function) and endocrine system (reduced production of testosterone and insulin). Frailty Index – 0.275.

Gerontological diagnosis: Early (accelerated) aging [+13 yrs – in 42 yrs wear of functional system of patient corresponds to 55 yr, onset of male climacteric status], type of combined aging (cardio-vascular, endocrine and neuro-psychological).Treatment: complete course “Golden Vial”.Treatment results:

2005 yr 2006 yr 2007 yr 2008 yr 2009 yr 2010 yr

BA>CA = + 13 yr Frailty Index = 0.275Undergone complete program of “Golden Vial”

BA=CA Frailty Index = 0.125Improvement of condition of functional system

BA<CA= − 3 yr Frailty Index =0.050Functional systems - normal Patient married

BA<CA= − 5 yr Frailty Index =0.025Functional systems – normalPatient had male child

BA<CA= − 6 yr Frailty Index =0.025Functional systems – normal

BA<CA= − 6 yr Frailty Index =0.025Functional systems – normal

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SUMMARYAging – this is decrease of stem cells reserve in organs and human tissues.For true rejuvenation need to increase stem cells reserve in human organs and tissues.Aging Disease: Arthrosclerosis and arterial hypertension, Chronic ischemic heart disease, myocardial infarction, heart failure, stroke, cancer, autoimmune disease, pneumonia and respiratory failure, stomach ulcers and duodenal bleeding, perforation, penetration and malignant diseases, blindness, deafness, fracture, osteomalacia, joint diseases, renal disease, liver, diabetes mellitus, asthenia, depression, senile dementia, Alzheimer’s disease.Reason for aging diseases: reason of aging disease is as aging itself the reduction of stem cells reserve in organs and human tissues.In prophylactics of aging diseases stem cells reserve is required to be increased in organs and tissues of the human.Aging – it is decrease of stem cells reserve in organs and tissues of the human. For true rejuvenation need to increase stem cells reserve in human organs and tissues.Chronological age – this is age as per birth of date.

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Biological age – this is “level of age wear” of our body.Standard quantitative characteristic of aging is difference between biological aging and chronological aging.Standard qualitative characteristic of aging is Frailty Index evaluating age damage and deficit function. More higher the Frailty Index lesser the quality of life and life span.Important indicator of true rejuvenation – period of healthy life. Successful aging – this is absence of disease and physical disability, continued ability to assimilate new information and physical activity, active part in family life and society. Successful aging – this is active longevity “Golden Vial” – it is program is transplantation of all types stem cell without any exception which is necessary for complete renewal of functional system and rejuvenation. Program includes 240 types of fetal stem cells transplantation and special complex bio-creams which provides regeneration of hair, skin and nails

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Results of rejuvenation of the hand, neck, décolleté and face skin in women, undergone program “Golden Vial”, according to international assessment scale GAIS (Global Aesthetic Improvement Scale): Evaluation of doctor/patient:Optimal cosmetic result of the given patient – 80.2/78.9%Significant improvement but not complete correction – 8.5/9.7%Improvement but additional correction required – 11.3/11.3% (women with deformities type of aging in face)No changes condition is same as before procedure – 0/0Deterioration in comparison with initial condition – 0/0Earliest to start the rejuvenation program “Golden Vial” the lesser probability of aging disease development.

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Anticipated result of true rejuvenation with the help of rejuvenation program “Golden Vial”:For women :30-39 yrs – to15.6 yrs younger40-49 yrs – to 15.8 yrs younger50-59 yrs – to 13.6 yrs younger60-69 yrs – to 10.8 yrs younger70-80 yrs – to 4.9 yrs youngerFor men:30-39 yrs – to 16.4 yrs younger40-49 yrs – to 16.5 yrs younger50-59 yrs – to 12.6 yrs younger60-69 yrs – to 11.7 yrs younger70-80 yrs т – to 5.4 yrs younger

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Our publication THE AGING: THEORY OF THE BODY STEM-SPACES DEPLETIONO.L. Kukharchuk, V.V. Radchenko, B.M. Gandhi, A.B. Padma Priya, A.O. Kukharchuk, Amol P. GitteEmProCell Clinical Research Pvt. Ltd., Mumbai, IndiaThe review contains an analysis of basic information about the principles and methods of a new direction in the treatment of human diseases named as regenerative medicine, basic information on stem-spaces of organism, provides information on the depletion of stem cells resources in the aging, and presented authors own theory of the aging mechanisms.Key words: stem cell, stem-spaces, immune system replacement, aging, regenerative medicine.

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Kukharchuk O.L., Radchenko V.V., Gandhi B.M., Padma Priya A.B., Kukharchuk A.O., Amol P. Gitte. “The aging: theory of the body stem spaces depletion” (2011) Medicine Today and Tomorrow 50-51 (1-2) 292-310 (Eng).Kukharchuk A.L., Radchenko V.V. “Embryonic pluripotent progenitor cells, immunological tolerance, and aging of organisms” (2008) Transplantology 10 (1) 51-54 (Rus).RadchenkoV.V., Kukharchuk A.L., Sirman V.M. “Embryonic pleuripotent progenitor cells, immunological tolerance, autoimmune diseases and aging of organism”(2003) Transplantology 4 (1) 225–228 (Rus).Radchenko V.V., Kukharchuk A.L. “Influence of embryonic pleuripotent progenitor cells on intensiveness and structure of tissue fibrinolysis in the aorta of old rats” (2003) Herald of Biology and Medicine Problems 5 (3) 53-56 (Rus).Radchenko V.V., Kukharchuk O.L. “Influence of embryonic pluripotent progenitor cells on proteolysis intensiveness in old rats aorta tissues” (2003) Medical Chemistry (21) (9) 32-37 (Ukr).Radchenko V.V., Kukharchuk A.L. “Transplantation of allogenic embryonic pleuripotent progenitor cells for the correction of arterial pressure regulation dysfunction: Assesment of effectiveness in experiment on rats with genetically determined arterial hypertension like position of theory of stem space organism depletion” (2003) Medicine Today and Tomorrow (7) 15-22 (Rus).Kukharchuk A.L., Radchenko V.V., Sirman V.M. “Aging, stem spaces and immune system” (2003) International Journal Immunorehabitation 5 (2) 134 (Rus).Kukharchuk O., Radchenko V.V., Padma Priya A.B., Kukharchuk A.O., Pranav Anam “Stem cell therapy in nephrology” (2012) Medi-focus 12 (1) 1-7 (Eng).Kukharchuk O., Salyutin R., Kukharchuk A., Radchenko V, Anand Baskaran Padma Priya, Pranav Anam “ Efficiency of human fetal liver hematopoietic stem cells (hFL-HSC) transplantation in treatment of chronic limbs ischemia (CLI)” (2012) J Cell Sci Ther 3 (8) 59 (Eng).Kukharchuk O.L. “Ukrainian experience of fetal stem cells transplantation for severe human diseases treatment: 13 years of clinical applications in legal field” (2012) J Cell Sci Ther 3 (8) 27 (Eng).Kukharchuk A.L., Palaynica S.S., Bikhunov V.L. “Diabetes mellitus, regenerative medicine and immunological tolerance” (2008) Transplantology 10 (1) 44-50. (Rus).

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Kukharchuk A.L., Radchenko V.V. “Influence of embryonic progenitor cells transplantation on the reproductive system of old male rats” (2007) Transplantology 9 (1) 142-146 (Rus).Skavulyak A.N., Kukharchuk A.L. “The study of mechanism neurogenesis dysfunction as the cell therapy basis for schizophrenia” (2007) Transplantology 9 (1) 263-269 (Rus).Kukharchuk O.L., Ganja T.M. “Stem cells of fetal liver: identification problem and prospects of application in practical medicine” (2007) Transplantology 9 (1) 147-150 (Ukr).Kukharchuk A.L., Palyanica S.S. “Theoretical basis for neutral stem cells application in treatment of the brain ischemic lesions” (2007) Transplantology 9 (1) 203-208 (Rus).Kukharchuk A.L., Radchenko V.V., Sirman V.M. “Evaluation of effectiveness of various types of embryonic brain cells transplantation in rats with experimental mode of hemi-Parkinson’s” (2004) Transplantology (7) (No.3) (Rus).Kukharchuk A.L., Radchenko V.V., Sirman V.M. “Regenerative medicine: Directions, achievements problems and prospects. Part IІ: Stem spaces” (2004) Ukrainian Medical Annals 41 (3) 99-107 (Rus).Kukharchuk A.L., Radchenko V.V., Sirman V.M. “Regenerative medicine: Directions, achievements problems and prospects. Part I: Principles and Methods” (2004) Ukrainian Medical Annals 40 (2) 70-79 (Rus).Kukharchuk A.L., Radchenko V.V., Sirman V.M. “Stem cells: experiment, theory, clinic. Embryonic, mesenchymal, neural, and hematopoetic stem cells” (2004) Chernivtsi: Gold Litavry, 505 (Rus).Kukharchuk O.L., Radchenko V.V., Sirman V.M., Sagach V.F. “Influence of allogenic embryonic pleuripotent progenitor cells transplantation on the systemic arterial pressure dynamic in spontaneous hypertensive rat” (2003) Journal Physiology, 49 (4) 68-72 (Ukr).Kukharchuk A.L., Radchenko V.V., Sirman V.M., Radchenko M.R., Dyba S.A. “Achievements, problems and prospects of regenerative medicine” (2003) Transport Ukraine Medicine 7 (3) 87-100 (Rus).

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