golden age for agrobiotechnology kim, byung-dong seoul national university & center for plant...
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Golden Age for AgroBiotechnology
Kim, Byung-Dong
Seoul National University &
Center for Plant Molecular Genetics and Breeding Research
Prediction by Nikkei Business
Most Promising Technologies in early 21C
Biotechnology & medicine - 1013 yen in 2010
Information Appliances & Computer
Environment
GMO
Mass production by 2001
Commercialization by 2006
농업적 형질
질적 형질
식물 화학 공장신제품
1995 2000 2005 2010
[그림 2] 바이오 테크놀로지 발전의 3단계
Current Topics in Transgenic Plants
QTLAFLP병저항성RFLP
웅성불임
병저항성
SSR
BAC library
생산량
품질향상
ESTs
Physical/Genetic map
Microarray
Functional Genomics
Transformation
Genome Projects
신종자 생산
BREEDING PROGRAM
Gene cloning
Plant genomics areaAquaculture
Arabidopsis
Barley
Brassicas
Compositae
Cotton
Edible Legumes
Forage & Turfgrass
Forest Tree
Fruit/Nut Tree
Lolium
Grape
Legumes
Maize
Ornamentals
Rice
Solanaceae
Sugarcane
Sugar Beet
Abiotic Stress
Apomixis
Bioinformatics/Computers
Comparative Genomics
Databases, Gene Systematics, and Nomenclature
Functional Genomics
Large-Insert DNA Libraries and Their Applications
Molecular Markers for Plant Breeders
Organellar Genetics
Plant Alien Introgression
Plant Cytogenetics
Plant Interaction with Pests and Pathogens
Plant Transformation
Plant Transgene Genetics
Polyploidy
Statistical Methods
Transcriptional Profiling
The Goals of Genome Science
1. Integrated Web-based database and research interface
2. Physical and genetic maps of the genome
3. Ordering of genomic and expressed gene sequences
4. Annotation of the complete set of genes encoded within a genome (ORFeom)
5. Atlases of gene expression (Transcriptome)
6. Functional data, including biochemical and phenotypic properties genes
(Proteomics, Functional and Structural genomics, Metabolomics)
7. DNA sequence diversity (SNP, LD, QTL)
8. Comparison with other genomes (Comparative genomics)
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86 87 88 89 90 91 92 93 94 95 96 97 98
[ 백만 ]
염기
[ 그림 6] DNA 염기서열 정보의 증가 ( 세계유전자은행 )
Arabidopsis
2000.12.
Human.
2001.2.
Rice
2002.4.
SNP DNA Chip
Proteomics
Genomic DNA
EST Protein sequenc
e
Gene Function
3D Structu
re
Comparative Genomics
Microbiology
Database Analysis
Bioinformatics : Data is King
http://ars-genome.cornell.edu http://ukcrop.net
Major resources for plant genomics
국내 식물유전체 연구사업
1. 작물기능유전체사업단
2. 바이오그린 21: 농업생명과학원 - 벼 , 배추
3. 국가유전체정보센터 : 자생식물사업단 - 고추 , 인삼
고추의 고추의 Genetic linkage map Genetic linkage map 작성작성
Promoter Promoter 연구연구 유전자 기작 연구유전자 기작 연구 Database searchDatabase search
내병성 관련 유전자고추 과색 관련 유전자내병성 관련 유전자
Phenylalanine (-5.0)
Cinnamate(+5.8)
CHS (+3.3)
Benzoicacid (+2.0)
Hydroxybenzoicacid (-11.1)
Protocatechoicacid (-3.2)
Gallicacid (+2.0)
Ellagicacid (-33.3)
Cinnamateglucose (+2.0)
Ethyl cinnamate (-7.7)
Naringenin
F3H (+3.2)
Dihydrokaempferol(-9.1)
ANS (+6.7)
Pelargonidin (+9.46)
Kaempferol
DFR (1.9)
Leucopelargonidin(-25)
ANS (+6.7)
Catechin(condensed
tannins)(-25)
GT1 (+8.3)
Pelargonidin glucoside (+255)Cyanidin glucoside (+2.1)
GT1 (+8.3)
Quercetin Dihydroquercetin(-2.4)
Cyanidin
Leucocyanidin(-2.7)
Naringenin chalcone
CHI (+4.3)
Coumarate(+3.2)
Coumaroyl - CoACoumarin(+5.5)
Hydroxycoumarin(+5.44)
CaffeateCaffeoyl
CoA
Chlorogenicacid
+ Quinicacid (-4.2)
Coumaraldehyde(+5.86) Coumaryl alcohol (+3.29)
CAD(+8.8)
CCR (+3.6)
Ferulate(+3.9)
Hydroxyferulate(+2.4)
Sinapate(+3.8)
SinapoylCoA
Sinapaldehyde(+2.4)
Sinapyl alcohol (+5.8)
CCR (+3.6)
CAD (+8.8)
OMT (+2.6)
OMT (+2.6)
GT2 (+3.7)
GST (+2.8)vacuoleGST (+2.8)
Kaempferolglucoside (+2.1)
Catechol(+2.0) Anthranilate (-2.3) Gentisic
acid (-3.2)
Vanillic acid (-12.5)
Glucogallin(hydrolyzable tannins)(-4.3)
PAL
Coumarateglucose (+9.6)
Quercetinglucoside (-7.7)
Methyl cinnamate (-5.3)
FeruloylCoA
Coniferaldehyde
Coniferylalcohol
CCR (+3.6)
CAD (+8.8)DFR (1.9)
Scopoletin(+5.31)
Phenylalanine (-5.0)
Cinnamate(+5.8)
CHS (+3.3)
Benzoicacid (+2.0)
Hydroxybenzoicacid (-11.1)
Protocatechoicacid (-3.2)
Gallicacid (+2.0)
Ellagicacid (-33.3)
Cinnamateglucose (+2.0)
Ethyl cinnamate (-7.7)
Naringenin
F3H (+3.2)
Dihydrokaempferol(-9.1)
ANS (+6.7)
Pelargonidin (+9.46)
Kaempferol
DFR (1.9)
Leucopelargonidin(-25)
ANS (+6.7)
Catechin(condensed
tannins)(-25)
GT1 (+8.3)
Pelargonidin glucoside (+255)Cyanidin glucoside (+2.1)
GT1 (+8.3)
Quercetin Dihydroquercetin(-2.4)
Cyanidin
Leucocyanidin(-2.7)
Naringenin chalcone
CHI (+4.3)
Coumarate(+3.2)
Coumaroyl - CoACoumarin(+5.5)
Hydroxycoumarin(+5.44)
CaffeateCaffeoyl
CoA
Chlorogenicacid
+ Quinicacid (-4.2)
Coumaraldehyde(+5.86) Coumaryl alcohol (+3.29)
CAD(+8.8)
CCR (+3.6)
Ferulate(+3.9)
Hydroxyferulate(+2.4)
Sinapate(+3.8)
SinapoylCoA
Sinapaldehyde(+2.4)
Sinapyl alcohol (+5.8)
CCR (+3.6)
CAD (+8.8)
OMT (+2.6)
OMT (+2.6)
GT2 (+3.7)
GST (+2.8)vacuoleGST (+2.8)
Kaempferolglucoside (+2.1)
Catechol(+2.0) Anthranilate (-2.3) Gentisic
acid (-3.2)
Vanillic acid (-12.5)
Glucogallin(hydrolyzable tannins)(-4.3)
PAL
Coumarateglucose (+9.6)
Quercetinglucoside (-7.7)
Methyl cinnamate (-5.3)
FeruloylCoA
Coniferaldehyde
Coniferylalcohol
CCR (+3.6)
CAD (+8.8)DFR (1.9)
Scopoletin(+5.31)
Combining Metabolic and Gene Expression Profiling
Phenylalanine (-5.0)
Cinnamate(+5.8)Hydroxybenzoic
acid (-11.1)Protocatechoic
acid (-3.2)
Ellagicacid (-33.3)
Ethyl cinnamate (-7.7)
Naringenin
Dihydrokaempferol(-9.1)
DFR (1.9)
Kaempferol
Leucopelargonidin(-25)
DFR (1.9) Catechin(condensed
tannins)(-25)
Quercetin Dihydroquercetin(-2.4)
Cyanidin
Leucocyanidin(-2.7)
Naringenin chalcone
Coumarate(+3.2)
Coumaroyl - CoACoumarin(+5.5)
Hydroxycoumarin(+5.44)
CaffeateCaffeoyl
CoA
Chlorogenicacid
+ Quinicacid (-4.2)
SinapoylCoA
vacuole
Catechol(+2.0) Anthranilate (-2.3) Gentisic
acid (-3.2)
Vanillic acid (-12.5)
Glucogallin(hydrolyzable tannins)(-4.3)
PAL
Coumarateglucose (+9.6)
Quercetinglucoside (-7.7)
Methyl cinnamate (-5.3)
Benzoicacid (+2.0)
Gallicacid (+2.0)
Cinnamateglucose (+2.0)GT2 (+3.7)
FeruloylCoA
Coniferaldehyde
Coniferylalcohol
CHS (+3.3)
F3H (+3.2)
ANS (+6.7)
Pelargonidin (+9.46)
ANS (+6.7)
GT1 (+8.3)
Pelargonidin glucoside (+255)Cyanidin glucoside (+2.1)
GT1 (+8.3)
CHI (+4.3)
GST (+2.8)GST (+2.8)
Kaempferolglucoside (+2.1)
CCR (+3.6)
Coumaraldehyde(+5.86) Coumaryl alcohol (+3.29)
CAD(+8.8)
Ferulate(+3.9)
Hydroxyferulate(+2.4)
Sinapate(+3.8)
Sinapaldehyde(+2.4)
Sinapyl alcohol (+5.8)
CCR (+3.6)
CAD (+8.8)
OMT (+2.6)
OMT (+2.6)
CCR (+3.6)
CAD (+8.8)
Scopoletin(+5.31)
Phenylalanine (-5.0)
Cinnamate(+5.8)Hydroxybenzoic
acid (-11.1)Protocatechoic
acid (-3.2)
Ellagicacid (-33.3)
Ethyl cinnamate (-7.7)
Naringenin
Dihydrokaempferol(-9.1)
DFR (1.9)
Kaempferol
Leucopelargonidin(-25)
DFR (1.9) Catechin(condensed
tannins)(-25)
Quercetin Dihydroquercetin(-2.4)
Cyanidin
Leucocyanidin(-2.7)
Naringenin chalcone
Coumarate(+3.2)
Coumaroyl - CoACoumarin(+5.5)
Hydroxycoumarin(+5.44)
CaffeateCaffeoyl
CoA
Chlorogenicacid
+ Quinicacid (-4.2)
SinapoylCoA
vacuole
Catechol(+2.0) Anthranilate (-2.3) Gentisic
acid (-3.2)
Vanillic acid (-12.5)
Glucogallin(hydrolyzable tannins)(-4.3)
PAL
Coumarateglucose (+9.6)
Quercetinglucoside (-7.7)
Methyl cinnamate (-5.3)
Benzoicacid (+2.0)
Gallicacid (+2.0)
Cinnamateglucose (+2.0)GT2 (+3.7)
FeruloylCoA
Coniferaldehyde
Coniferylalcohol
CHS (+3.3)
F3H (+3.2)
ANS (+6.7)
Pelargonidin (+9.46)
ANS (+6.7)
GT1 (+8.3)
Pelargonidin glucoside (+255)Cyanidin glucoside (+2.1)
GT1 (+8.3)
CHI (+4.3)
GST (+2.8)GST (+2.8)
Kaempferolglucoside (+2.1)
CHS (+3.3)
F3H (+3.2)
ANS (+6.7)
Pelargonidin (+9.46)
ANS (+6.7)
GT1 (+8.3)
Pelargonidin glucoside (+255)Cyanidin glucoside (+2.1)
GT1 (+8.3)
CHI (+4.3)
GST (+2.8)GST (+2.8)
Kaempferolglucoside (+2.1)
CCR (+3.6)
Coumaraldehyde(+5.86) Coumaryl alcohol (+3.29)
CAD(+8.8)
Ferulate(+3.9)
Hydroxyferulate(+2.4)
Sinapate(+3.8)
Sinapaldehyde(+2.4)
Sinapyl alcohol (+5.8)
CCR (+3.6)
CAD (+8.8)
OMT (+2.6)
OMT (+2.6)
CCR (+3.6)
CAD (+8.8)
Scopoletin(+5.31)
CCR (+3.6)
Coumaraldehyde(+5.86) Coumaryl alcohol (+3.29)
CAD(+8.8)
Ferulate(+3.9)
Hydroxyferulate(+2.4)
Sinapate(+3.8)
Sinapaldehyde(+2.4)
Sinapyl alcohol (+5.8)
CCR (+3.6)
CAD (+8.8)
OMT (+2.6)
OMT (+2.6)
CCR (+3.6)
CAD (+8.8)
Scopoletin(+5.31)
Coumaraldehyde(+5.86) Coumaryl alcohol (+3.29)
CAD(+8.8)
Ferulate(+3.9)
Hydroxyferulate(+2.4)
Sinapate(+3.8)
Sinapaldehyde(+2.4)
Sinapyl alcohol (+5.8)
CCR (+3.6)
CAD (+8.8)
OMT (+2.6)
OMT (+2.6)
CCR (+3.6)
CAD (+8.8)
Scopoletin(+5.31)
Green > Red Red > Green
Metabolic and gene expression profiling in the phenylpropanoid and related pathways. Background color represents metabolism of flavonoids (yellow), lignin (blue), coumarin (gray), tannins (green) and cinnamic acid derivatives (pink). Genes analyzed for their expression by microarrays are indicated in capital and italic and their expression R/G expression ratio in parentheses. Metabolites or genes showing elevated expression in the red stage compared to green or visa versa is depicted in red or green colors, respectively, those not detected are in black and ones detected but with no significant change in expression are depicted in black and underlined. PAL- phenylalanine ammonia lyase; CHS- chalcone synthase; CHI- chalcone flavanone isomerase; F3H- flavanone-3-hydroxylase; DFR- dihydroflavonol 4-reductase; ANS- anthocyanidin synthase; GT- glucosyltransferase; GST- glutathione S-transferase; OMT- O- methyl transferase; CCR- cinnamoyl CoA reductase; CAD- cinnamyl alcohol dehydrogenase. Dashed lines indicated multiple steps in the pathway.
가상현실을 이용한 식물대량생산시스템 구축
1. 대량생산체계
종자 ( 조직배양단계 ) -> 육묘단계 -> 영양생장단계 -> 생식생장단계
2. 실현 방법
모델링 및 측정 기법
3. 관찰 수준
거시적 차원 : 식물생육 , 식물형태 , 광합성량 , 병해충 반응결과 미시적 차원 : 세포분열 , 양수분이동 및 분배 식물생산시스템 : 광환경 추정 , 식물 ( 군락 ) 생산성 , 시스템 설계
4. 단계별 주요 관찰 대상
종자 ( 또는 조직배양 단계 ): 발아 ( 재분화 ) 육묘단계 : 형태형성 영양생장단계 : Biomass 생식생장단계 : 성분
5. 연구 동향
식물생장예측 --> 모델링 연구
식물형태예측 --> 모델링 연구
식물생산성예측 --> 모델링 + 3 차원 그래픽 연구
식물대량생산시스템설계 --> 가상현실 시스템 일부 연구
결론
1. 21 세기는 생명공학시대
2. 다국적 대기업에 의해 독점되는 추세
3. 게놈사업 , 바이오칩 , 대형화 – 자동화 – 신속화 ,
정보화
4. BT, IT, NT 의 접목에 의한 고도지식산업 창출가능