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GuidelinesforPreparingandSubmittingPooledLibraries

totheOxfordGenomicsCentreThere are a series of basic steps to follow to ensure safe delivery of samples andtimelyinitiationofyourproject.Thisguidelinewillwalkyouthroughthesestepsanddirectyoutotheappropriatedocumentstoreadforyourparticularprojectneeds.

Failuretofollowtheseguidelinesandincorrectsubmissionofsampleswill:

1. Delaytheinitiationofyourproject2. Putyoursamplesatrisk3. Resultinadditionalchargesbeingappliedtoyourproject

Ifindoubtpleaseconsultyourprojectmanager.SampleSubmissionOverviewTheschematicbelowisdesignedtogiveyouanoverviewofthestepsthatyouarerequired to take to ensure the necessary amount of material is provided in thecorrectcontainer,attherighttemperatureandwithalltherequiredpaperwork.Following these guidelines will protect your samples, avoid delays in projectsinitiationandpreventadditionalchargesforprocessing.

1.QCLibraries

2.PlaceintheCorrectContainer

3.CompleteRequiredPaperWork

4.Package

DelivertoWHG ShipSamplestoWHG

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1. LibraryQCGuidelinesToensuresuccessfulpreparationoflibrariesasetofguidelineshavebeenpreparedthatdescribetheminimumamountandqualityofmaterialrequired.

1. Pre-preparedLibrariesQCTheseguidelinescanbefoundonourwebsitehttp://www.well.ox.ac.uk/ogc/documents

2. PlaceLibrariesinTubesTubes:PleaseuseEppendorfLoBind1.5mlmicrocentrifuge tubes.Tubesshouldbepackaged together ina sealedcontainer (egsmallbagorbox)beforeputtingontodryice.Thesealedcontainermustbelabeledwithyournameandquoteorprojectnumber.

Fortubes,ensurealllibrariesareuniquelylabeledandthelabelsmatchthosegivenonthesamplesubmissionform(Eg:Input1,Input2).Customer Sample Name Input 1 Input 2

If the libraries are in thewrong containers,we reserve the right to returnat yourcostortochargeaprocessingfee.Onlyonealiquotofeachlibraryshouldbesubmittedunlessbypriorarrangement.

3. CompletetheAppropriatePaperWork

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4. PackagingLibraries

1. All libraries should be on dry ice if being shipped or if to be left inWHG

receptionarea.SubmittingSamplesforthosewithAccesstoWHGLab3FreezersWhendroppingoffyoursamples

1. Thereisadesignatedfreezerforsampledropoff.ThefreezerislocatedinthefirstbayonthesouthsideofLab3.AplanofLab3andthe locationofthefreezercanbeseeninAppendix1.

2. Samples should be left in this designated area between 9am and 1pm onstandardworkingdays.

This freezer will not be checked other than between these times so werecommendonlytoleavesamplesbetween9amand1pminorderthatyoursamplesarenot lostordefrosted if the freezer isopenedor fails after thistime.

3. The labeled plate or box/bag of tubes should be placed in appropriatedrawer;eithertheoneforDNAortheoneforpre-preparedlibraries.

4. OntopofthefreezerthereisanareainwhichtoleaveRNAsamplesondryice.

After 1pm, nobody will return to check for samples until the followingworkingday.Ifyouareunabletodropoffyoursamplesbetween9amand1pm,please arrange for a colleague todo soorwaituntil the followingworkingday.

1. DeliverorShipLibraries

SubmittingSamples-drop-offpointinWHGreception

1. It will be possible to leave libraries in a cupboard in reception (see imagebelow)between9amand1pmonanyworkingday- this area is not secureandwillbecomewarm,pleaseputallsamplesondryice.

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2. After 1pm, nobody will return to check for samples until the followingworkingday.

3. If you are unable to ship or dropoff your samples in this time slot, pleasewaituntilthenextworkingdayoraskacolleaguetosendthesamplesinyourabsence.

SubmittingSamples-shippingsamples

1. Samples should only be shipped between Monday and Wednesday (or 2workingdaysbeforethestartoftheweekendinthecaseofbankholidays).

2. Allsamplesshouldbeondry ice.Somecouriershavespecificguidelines forshipping samples on dry ice and should be contacted for details prior topackagingupyoursamples.

3. Pleasesendto:

High-ThroughputGenomics(Sequencing),WellcomeCentreforHumanGenetics,RooseveltDrive,Oxford.OX37BN

High-throughputSampleSubmissioncupboard

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Appendix1

Lab3entranceLab3entrance

Submissionfreezer