Heat Stable Bacillus Spores as Vaccines

Professor Simon CuttingSporeGen Ltd.School of Biological SciencesRoyal Holloway University of LondonEgham, SurreyTW20 0EX, UKs.cutting@rhul.ac.uk

Ethical Non-pathogenic Storage Robust (heat-stable), suitable for 3rd World Cost Straightforward production Route Oral edible vaccine. Intra-nasal (killed spores)

Licensed OTC drug (Bacillus clausii)100m doses sold/yr in Italy by Sanofi-Aventis

Bacillus spores

The Concept of Spore Vaccines

Stomach

Spores survive intact

vegetative Bacillus killed

Small Intestine

Nutrient rich

Spore germination &

dissemination to GALTColon

Re-sporulation

GIT

Excretion of sporesinto the soil

Symbiotic interactions with plants

Consumption ofplants and spores

spores

vegetative cells

Bacillus as a gut commensal

NattoB. subtilis var. Natto

Japanese staple

BioPlus 2BSpores of B. subtilis & B.

licheniformisAnimal feed

Christian Hansen (Denmark)

Bacillus Spores: Probiotics, Functional Foods and Feed Supplements

Main Street Gourmet (USA)obtained from Isabellas Healthy Bakery

ActivateProbiotic muffins impregnated with spores

1.E+00

1.E+01

1.E+02

1.E+03

1.E+04

1.E+05

1.E+06

1.E+07

1.E+08

1.E+09

1.E+10

1 2 3 4 5 6 7 8

HU58

Protexin

Control

Before cooking

0 7 14 28 42 56 84

Days

Spor

es co

unt /

gra

m

Survival of spores incorporated in biscuits (McVities) after 84 days

These are background countsAnd are Bacillus spores present in your biscuit mix. The biscuit mix is not sterile and data shows that cereals, wheat etc have a basal level of spore counts.

Starting CFUHU58 Tube 1 + 1kg mix = 6.57 X 108 spores/gPXN21 Tube 1 + 1kg mix = 1.33 X 109 spores/g

235oC 8 min.

+Killed Spore

Killed Spore

Antigen(protein or virus)

Vaccinate

Expression on Spore CoatRecombinant (GMO)

Adsorption onto the Spore CoatNon-recombinant/non-GMO

Vaccinate

Spore gene X + Antigen gene Y

X Y

rec spore

SporeVax

Strengthening Animal Production and Health through the Immune Response

The European research project SAPHIR aims to develop innovative, safe , affordable and effective vaccine strategies effective against endemic pathogens responsible for high economic losses in livestock.

SporeGen is collaborating on the development of spores for 2nd generation oral/feed vaccines for Necrotic Enteritis

Shrimp Aquaculture(>$11bn/annum)

Penaeus monodonBlack Tiger Shrimp

Massive reduction in production to disease

Litopenaeus vannameiWhiteleg shrimp

Originally from Central/South AmericaIntroduced to Asia to replace P.

monodonBut stock now suffering epidemics of

disease

Shrimp Pathogens

About 30 viral diseases of shrimps White Spot Syndrome Virus (WSSV) is universally considered the worst

Expression on Spore CoatRecombinant (GMO)

Vaccinate

Spore gene X + VP28/VP26

X Y

rec spore

Spores display VP28 and VP26capsid proteins

Shrimps that eat the vaccine in feed pellets are protected against White Spot Syndrome Virus

Mortality rate (%)

Days post challenge

Protection trial: feeding during 14 days, then challenge

SPR at day 14 by CotB-VP28 at 1 x 10*9 cfu/g = (1-19.23/100)*100% = 81% SPR at day 14 by CotB-VP28 at 1 x 10*11 cfu/g = (1-10/100)*100% = 90%

0102030405060708090

100

1 2 3 4 5 6 7 8 9 10 11 12 13 14

UnchallengedUntreatedPY79 (1 x 10*9 cfu/g)CotB-VP28 (1 x 10*9 cfu/g)CotB-VP28H (1 x 10*11 cfu/g)

Does it work?

Phase 1/2a clinical trialRecruiting nowin Germany

www.cdvax.org

SporeGen (www.sporegen.com)

Operating in UK and Vietnam

SporeVax for development of human and animal vaccines

Spore mediated delivery of heat-stable feed enzymes (phytase, xylanase etc)

Clostridium difficilevaccinesnovel treatmentsdiagnostics

Helicobacter pyloritreatments

Animal feed productsHanoi, Vietnamhttp://biospring.com.vn

+ SGF + SIF

72 kDa

72 kDa

1 2 3 4

41 kDa

A Cytoplasmic expression (rrn0-GST-Cpa247-370)

B Spore coat expression (CotB-GST-Cpa247-370)

58 kDa

41 kDa

58 kDa

41 kDa

15 kDa

15 kDa

C. perfringens

A (Oral) B (Nasal)

Cpa (Clostridium perfringens) toxin A expressed on spores

3.6 g/dose; 2 X 1010 spores 0.15 g/dose; 2 X 109 spores

Seroconversion, IgG

Chart3

4040.214042.340.3023.216338907905.360206561822.97873957

406641.3112.461.2019.69300576513.400409163717.369686654413.6303805758

37.97354.9295.641222.954745831720.758675798123.4416478273119.4987208581206.7500835338

4040402911.74937.4004.0424422064985.13130851562556.0593300985

Nave

PY79

GST-

PY79+ GST-

HT251

Days post primary vaccination

End-point titer

test IgG, IgA

0.2930.0340.2420.080.1430.1030.3170.016

0.2080.2170.2720.170.0260.1050.1940.286

0.240.210.2750.2530.1620.1530.1830.198

0.140.1640.2380.2410.0430.0820.1270.197

0.2450.2070.2730.2560.1620.1710.2110.274

0.1930.1760.2430.2440.0310.0620.1240.197

0.2350.2070.2710.2590.1630.1660.2060.243

0.2150.1970.2680.2650.1580.190.2040.22

0.4740.3570.3540.4010.02

0.480.4060.4530.5060.023

0.5020.4760.5050.5070.468

0.5190.5020.5030.5060.518

0.3020.0490.1720.1230.019

0.4080.2050.2620.2890.025

0.4370.3430.3340.4250.435

0.4950.4680.4310.4640.181

17-Sep

IgA_266_Nnasallung-IgA

12-80.6050.3950.2910.1880.1480.1070.0570.0630.060.060.0770.077Blank

12-70.5910.4710.3630.2230.1710.1260.8630.8680.7390.330.4710.344Pos

12-60.7640.5820.4170.2620.1740.1250.260.2050.1360.0830.1080.101Py-4

12-50.6980.5480.4180.2850.1980.1290.2620.1730.1340.080.1050.095py-3

12-40.9420.9380.8480.6890.4810.30.2180.1490.1220.070.0980.086py-2

12-30.4020.350.240.1680.1180.0920.2660.1770.1290.070.0970.098py-1

12-20.3450.3010.20.1310.1010.0890.1990.1260.1170.0720.1080.102naive3

12-10.6820.4630.3160.2220.1440.1230.2260.1250.120.0780.1110.1naive2

IgG-266-NnavePy12-1

0.2550.1870.1610.1060.1820.1310.1660.1870.1560.140.1771.131

0.1480.090.0830.0640.1020.0720.1020.1080.0940.0840.1020.999

0.0960.0620.0580.0480.0760.0490.0750.0750.0740.0580.0670.896

0.0940.0470.0460.0380.0640.0470.0780.0550.0610.0480.0540.664

0.0760.0430.0390.0360.050.0370.0460.0440.0490.0410.0420.449

0.0810.0410.0360.0330.0420.0360.0410.0380.0430.0290.040.279

0.0750.0420.0360.0330.0390.0360.0370.0350.0370.0340.0370.173

ip-pos1.4761.4431.3371.2021.1341.0710.8980.7170.0610.0540.070.088

1/4001/51200

18-Sep

IgA_266_Nnasallung-IgA

12-80.7610.5010.3460.2240.1780.140.1180.1250.1250.1250.1190.127Blank

12-70.6910.4710.3420.2370.2230.1421.1231.0680.9730.8030.5750.418Pos

12-60.7320.6060.4150.2960.1960.1560.270.2210.1810.1490.1280.134Py-4

12-50.8120.6690.4790.360.2230.1690.2230.2040.1680.1480.1240.112py-2

12-41.0811.0340.9210.7720.4280.3730.2280.1970.1580.1380.1130.113py-2

12-30.4510.3470.2420.1850.1010.1140.3260.2490.2070.1720.1460.139py-1

12-20.3920.2630.1920.1470.0590.1020.3170.2620.2170.1740.1510.131py-3

12-10.8790.5030.3210.2320.1040.1460.7960.6120.3920.2640.2180.196naive2

IgG-266-Npy12-1-paper12-1-serum

0.2050.1560.1910.2230.2020.250.330.8740.210.1710.1580.8921/40

0.1410.0750.0980.1220.1330.2030.2610.7760.1340.0830.0930.8181/80

0.1010.0550.0650.0890.0950.1250.1630.730.0950.0610.0630.6891/160

0.0730.0430.0490.0670.0650.0810.1110.5710.0720.0450.0430.5171/320

0.0540.0360.0380.0490.0490.0630.0710.430.0490.0370.0360.3621/640

0.0460.030.0310.0410.0420.050.0530.2870.0410.0330.0320.2251/1280

0.0370.0290.0290.0320.0350.0330.0420.1460.0340.0260.0280.146

IP-pos0.9690.9910.8450.8080.7230.5630.4910.2650.0440.0440.0490.061

1/800102400

neutralisation

NaCL

11-SepHT251IPnasalORAL

0.3540.2980.310.2010.30.2280.180.1180.3370.2710.2050.0861

0.3750.3320.3030.2120.1310.0170.0170.020.3480.3250.3050.3142

0.2990.3320.2750.2140.4570.4410.3760.2490.0130.0160.020.0283

0.2650.1890.1560.0830.3180.2430.1680.10.3840.3070.30.2654

ip-Pos0.140.0390.0170.0150.2870.2670.2260.1790.310.2720.2590.2625

0.2950.2490.2520.1910.2650.2270.190.1520.3350.2950.2820.2396

0.3240.2910.2920.260.3110.2940.2750.2380.0210.0130.0170.0227

0.3020.2940.4290.0460.270.1550.0230.0310.2990.2180.1180.0318

toxintriton-XNaCL

HT266IPnasalORAL

0.3450.3220.2730.2410.2910.2570.1780.0790.4510.3510.2850.3021

0.3230.2870.2580.2760.3480.290.2670.2290.3860.3360.2930.2792

0.3420.3080.2710.2630.3010.2830.2420.2160.3620.3130.2650.2583

0.3020.2880.2650.2350.3010.2730.2210.1940.3640.3340.2890.264

0.3470.3420.310.2850.2850.2350.1610.0710.3440.3090.2910.2835

0.3520.3290.3110.3140.310.2930.2610.2650.3590.3170.2890.2886

ip-Pos0.2030.0940.0250.0190.2980.2620.2270.1780.3430.2780.2210.1367

0.2150.3380.4140.0180.3930.290.2750.2830.380.3580.3450.3128

toxintriton-XNaCL

HT230ORALnasalIPtriton-Xtoxin

80.3180.3530.3670.3950.2090.2790.3190.3190.3820.4450.5180.379

70.2980.3050.3210.3560.3070.3040.3360.3590.5290.3220.3640.383

60.2160.3110.3090.3340.2330.2510.2650.2880.5360.2950.3230.385

50.4770.3790.2010.2850.1670.1950.2470.2650.530.0260.0230.124ip-Pos

40.2820.3090.3270.3710.3080.3320.4470.3770.4660.2870.3330.334

30.30.3210.3720.3650.3320.3730.3960.3890.4520.2610.2910.314

20.2950.370.3330.360.4540.4640.4830.4160.4570.3120.3450.341

10.360.3630.3840.40.2680.2950.3430.3470.3730.3320.3150.445

protein

NaCL

ip-pos0.0250.0230.0650.1120.1680.4240.1030.080.0820.1840.2080.257

0.120.1830.2250.2250.0840.1840.2180.2250.120.1810.2150.282

0.2230.2270.260.2530.0670.1670.2240.3180.060.1310.20.235

0.1590.1870.2360.2650.0660.1650.2160.2680.1260.2090.2110.25

0.0890.190.230.2680.080.1880.2410.2920.1560.210.2420.268

0.1880.1710.2080.2340.0650.1570.2060.2790.1090.1510.180.245

0.0780.1450.2210.2550.0970.180.2290.2590.1030.1750.220.253

0.1680.1180.2280.2680.1220.1810.2380.2990.1260.1860.210.205

BORATE

12-SepHt266oralnasalboratetritonX

80.0970.1850.2430.2960.0960.1750.2660.3130.0340.040.3460.372

70.1230.1920.2730.3030.1220.1970.2720.310.290.3710.370.38toxin

60.0910.1650.2410.2920.1010.1840.2670.3140.3130.3380.3480.351IP (1/400)

50.0780.1840.2490.2840.0870.1650.2510.2980.1770.2690.3120.349Py_Nasal

40.1110.2070.2660.2910.1060.1890.2610.2970.1590.2660.2870.32

30.0830.1690.2370.2820.1060.1760.2440.2910.1470.2250.30.329PY_Oral

20.0830.1690.2420.2870.2320.2880.3010.3070.1720.2090.2720.309

10.1080.2340.2790.30.2670.3030.3130.2930.1410.2120.2770.31612_1

13-Sep

HT230oralnasal

80.2380.2730.3360.3840.2090.2760.3390.410.0340.0270.420.443

70.1990.2610.320.370.210.2790.3380.3970.3910.4490.4550.454

60.2260.2710.3230.3710.2280.2760.3280.3640.140.3040.3750.417toxin-pos

50.250.2650.3260.3290.1490.2340.2980.3540.2860.3510.3760.386py-2

40.2350.2810.3280.3650.2310.3180.3660.3880.2660.3370.3870.411py-1

30.2160.2560.3180.3720.2680.3280.3640.3880.1530.2070.30.371py-2

20.2270.250.2970.3550.3150.3640.3790.4090.2410.2840.3340.376py-1

10.160.2520.3140.360.2210.2630.2940.3890.2580.3290.3710.443nave

13-Sep

HT251oralnasalboratetritonX

80.0350.1180.220.2940.0320.1240.2380.3250.0210.0270.4180.433

70.0320.0280.0340.1340.1420.2040.2930.3360.3960.4040.3960.414

60.1340.2050.2740.3060.1090.1720.2560.3310.070.2410.3180.374

50.1380.20.2690.3080.1310.1980.2480.3460.230.290.3280.371py-2

40.1450.2170.260.3830.1480.2010.2590.3180.2160.2640.3190.369py-1

30.0390.0310.0560.190.2070.3150.330.3910.1160.1710.2460.325py-2

20.1260.1920.2560.2940.0250.1010.2160.310.2030.2350.2860.342py-1

10.1070.1880.2530.3010.1360.2070.2950.3640.2690.2640.3090.362nave

13-Sep

IPHT251boratetritonX

80.0890.220.2730.2980.140.1890.2480.2980.0210.0220.3490.372

70.1640.2140.2550.290.150.2060.2740.2950.3330.340.3390.352toxin

50.1570.180.2330.2640.1540.2180.270.3050.1070.2160.2820.325IP

40.1820.2180.2540.2760.1240.180.2330.2810.1520.2060.2580.302py-2

30.1620.1970.240.2650.1350.1850.2360.2720.1350.1930.2540.307py-1

20.1760.1960.2430.2720.130.1840.2370.2770.1710.2130.2640.306py-2

10.1890.2030.2410.270.0440.1060.1640.120.1460.1920.2480.294py-1

py-30.1920.2150.2620.2810.1350.1780.2290.2820.1850.2380.2760.315nave

HT266HT230

13-Sep

oralproteinPY+protein

80.1050.1870.2370.3120.0470.0330.0280.070.0260.0270.3670.382

70.2330.2790.3070.3580.220.2490.3010.340.3670.3720.370.372

60.1860.2150.2770.3380.210.3020.3540.3790.1380.2250.2970.34

50.1750.2320.290.3610.1770.2370.3040.3350.1660.2350.2820.322py5

40.1820.2270.2910.3350.1860.2610.2990.3230.180.230.2730.307py-4

30.1350.1840.2430.3070.1440.2130.2990.3210.1870.2450.3030.338Py3

20.1840.2240.280.3240.1570.2190.2770.3030.1020.1650.2390.286py-2

10.2120.2480.30.3250.1670.2610.2890.3190.2420.2840.310.33nave

14-Sep

nasalproteinpy+pronegatriton

80.2840.3510.4110.4090.3410.3990.4070.3430.0120.0130.4280.451

70.3490.4010.4210.4240.2190.330.4230.3240.4690.4690.4640.468toxin

60.2630.3540.4110.4160.0370.150.1990.1480.1960.3910.4410.463IP

50.1970.4010.4260.4130.2130.3190.3780.3050.3310.3870.4190.442

40.230.3870.4170.4070.2290.3360.3880.330.3780.4020.4120.434py-nasal

30.2630.3460.3940.4010.190.3030.3620.3660.3490.3850.4140.434

20.2520.3520.4010.3970.2230.3250.3690.360.3820.4190.4320.444

10.3190.3690.410.4060.1780.2670.3710.3380.2050.2610.3430.385nave

14-Sep

IPproteinpy-2py+pronegatriton

py-10.2720.3610.3960.4360.3420.4040.430.4490.0150.0140.4780.543

70.2630.3690.3880.4320.3370.3890.4020.4470.4980.5140.5130.504

60.2680.3640.3630.4390.4280.4660.4740.4780.1640.340.4490.462

50.2530.3420.3710.4460.3580.3640.3960.4380.3470.4080.4310.462py-6

40.2290.3130.3380.3840.2820.3470.3850.4210.2860.3410.4250.412py-5

30.2820.3660.3820.4120.3480.3130.3650.4110.290.3490.3910.433py-4

20.2420.340.3640.390.2670.3280.3580.3960.2630.3560.3950.434py-3

10.2350.3170.3370.3970.2660.2770.3360.3820.2160.2830.3430.384nave

ip-py

nave0.3180.3370.3630.3690.2010.270.3240.3540.040.0280.0320.15Py+protein

0.1480.2280.2850.3320.2010.2840.330.3730.3510.4240.3990.404toxin

80.2130.2770.3150.3470.2080.2990.3430.3580.3510.4010.3950.396

Py-70.2410.3340.3660.3710.2070.3170.360.3750.180.2260.30.341nave

oral-60.2520.3080.3410.3590.2020.2730.3180.3480.1870.2030.2640.31

50.2280.2830.3260.3480.2020.2610.3110.3320.030.0240.3720.378control

40.2550.2990.3360.3460.2770.3120.3330.3480.3310.3770.3710.369toxin

30.1370.2270.2910.3270.3050.3190.3390.3550.140.2610.3270.347pip-pos

nasal py

13-Sepcompare Na_borate

Nasal0.0420.3750.3690.3740.016na

IP0.1470.4160.3870.4020.466tri

Py0.1560.4050.380.3970.401toxin

nave0.1150.4030.4010.4110.411toxin

Nasal0.040.0570.040.0280.007na

IP0.0070.010.0090.010.337tri

Py0.2440.1050.0690.0410.214toxin

nave0.2270.1220.1990.3920.406toxin

natritoxintoxin

control0.0390.6720.6250.634

IP0.1320.3560.4860.571borate

nave0.340.5110.5610.593

py0.3040.3840.5210.599

control0.0320.6490.5770.57

IP0.0380.1670.3570.469nacl

nave0.3890.4710.5180.551

py0.3080.4210.5030.57

0.050.0680.10.0560.0840.1150.0580.0830.1080.0190.0160.02

0.040.0610.0880.070.10.1210.050.0790.1090.0250.0160.011

0.0370.0570.0880.0630.0720.1020.0640.0840.110.0230.0160.016

0.0380.0590.0880.0490.070.0930.0560.0830.1090.0220.0390.028

0.0540.0790.1090.0450.0660.090.0480.0690.0930.0210.0190.018

0.0430.0620.0930.0570.0760.1020.0660.0830.1070.020.2560.264

0.0440.0640.160.0460.0680.0960.0660.0880.1130.020.1920.192

0.0550.0790.1110.1230.1390.1680.050.070.0970.0180.0150.02

0.1960.2180.2360.20.1630.2520.2370.2640.2820.0210.0210.023

0.1860.1960.210.2330.2220.2520.1850.220.2430.0230.0180.022

0.1630.1830.2040.2070.1930.2460.2480.2740.2810.0190.0340.033

0.1460.1650.1890.1630.1490.2190.2030.2320.2440.0220.1350.129

0.1760.1890.20.150.1260.1980.1650.2010.2140.020.0210.023

0.1490.1760.1930.2090.1690.2290.2290.2350.2390.0210.2590.276

0.1550.1770.2410.1740.1450.190.2250.2430.2480.0170.3010.299

0.190.2070.2250.2620.1950.2930.1990.2260.2310.0180.0290.028

lung_IgA

LUNGIgA

17-Sep

IgA_266_Nnasal

12-80.6050.3950.2910.1880.1480.1070.0570.0630.060.060.0770.077Blank

12-70.5910.4710.3630.2230.1710.1260.8630.8680.7390.330.4710.344Pos

12-60.7640.5820.4170.2620.1740.1250.260.2050.1360.0830.1080.101Py-4

12-50.6980.5480.4180.2850.1980.1290.2620.1730.1340.080.1050.095py-3

12-40.9420.9380.8480.6890.4810.30.2180.1490.1220.070.0980.086py-2

12-30.4020.350.240.1680.1180.0920.2660.1770.1290.070.0970.098py-1

12-20.3450.3010.20.1310.1010.0890.1990.1260.1170.0720.1080.102naive3

12-10.6820.4630.3160.2220.1440.1230.2260.1250.120.0780.1110.1naive2

19-Sep

plate-1HT230PY+pro

oral0.5410.3890.3150.2160.1740.1470.8580.850.8470.8420.7940.107

0.4120.2870.220.1670.1250.1080.6320.5510.4480.3110.2270.091

0.5450.390.2890.2170.1650.130.70.5820.4890.360.2950.446pool

0.4250.3170.2530.1920.1620.1410.8250.8040.7440.6290.5570.427

0.3080.2330.180.1440.1170.1010.2660.2320.20.1790.1560.489

0.3560.2640.2030.1630.1340.1090.4940.3660.2920.2190.1660.601

0.4950.3880.3160.2490.2050.1690.2940.2390.2040.1760.1440.743

0.5260.3960.2890.2290.1870.150.2850.2660.230.20.1770.778

plate-2HT251py-oral

oral0.7430.7480.6830.6840.6840.5970.3620.3150.2520.2070.1540.099

0.7680.7690.660.6750.6410.5980.3870.3340.3060.2410.2010.096

0.6320.5440.4610.3460.2770.1940.3290.3010.2540.2160.1870.413pool

0.6030.4370.3410.230.1730.1270.2790.230.2120.1840.1560.408

0.2250.1650.130.1040.0910.0850.3210.2510.2510.2140.1960.553

0.8250.80.7980.6980.6970.6260.2580.2180.1920.1730.1590.686

0.5680.3990.3090.20.1420.1150.2390.190.1830.1720.1510.773

0.7450.6930.7030.5990.5420.5070.2380.1930.1850.1750.1630.852

plate-3HT266pro-only

oral0.6640.6670.5970.5950.4740.3560.7950.7430.6620.6350.570.065

0.6460.5550.4180.3450.250.160.2590.2110.1580.1290.0870.052

0.5050.4120.2850.2210.1780.120.2560.2310.1830.1510.1160.438pool

0.3990.3010.2140.1710.1360.1030.2640.220.1760.1470.1080.428

0.3990.2850.2150.1620.1260.10.2130.1720.1390.1180.0980.408

0.5260.4350.3040.2320.1580.1180.2280.1730.1480.1230.1010.509

0.360.2820.2160.1610.1310.1090.3710.270.2160.170.1250.676

0.2870.2190.1710.1250.1130.090.1770.1580.1360.1190.0740.694

plate-4HT2230PY+pro

nasal0.6720.5680.4480.3280.2360.170.3540.2810.2290.1730.1290.05

0.4490.3070.2230.1560.1190.1020.6060.4920.3730.2650.1840.049

0.6470.5180.3850.2680.1850.1320.6450.5960.50.3960.2910.283

0.6080.480.3590.2470.1710.1250.4080.2850.2020.1510.1140.265

0.6660.5730.4680.3270.2220.160.7040.6590.6120.5270.4270.379

0.4970.3510.2580.1840.1360.1070.6080.4950.3810.2670.1950.519

0.6360.5090.3820.2620.1810.1320.5470.4220.3080.2270.1680.591

0.6550.5390.4270.2990.2110.1560.6540.6180.5410.4610.3480.623

plate-5HT251pro only

nasal0.7380.6830.6240.5560.4550.3370.2440.20.1630.1410.1180.079

0.6980.6220.530.4180.2960.1920.1630.1360.1140.10.0870.076

0.6430.5630.470.3480.2380.1550.1160.1020.0910.080.0740.249

0.6340.5320.4110.2820.1840.1210.1430.1190.1070.0920.0850.258

0.7310.6680.5990.5080.390.2690.1670.1340.1120.090.0840.39

0.7340.6880.6060.5080.3710.2430.1430.1240.1080.0920.0830.524

0.8060.7670.7380.7090.6730.6080.2830.1890.1440.1140.0920.576

0.7230.6140.5630.4440.3250.2230.2010.1760.1560.1230.1220.662

plate-6HT266-ip

0.1490.1190.110.1050.0990.0970.3660.3050.2320.1780.1520.078

80.2730.2020.1540.1260.1030.0870.2180.1980.1490.1240.1070.085

70.2160.1460.1180.0960.0810.0690.1760.1440.1220.1030.090.323pool

50.2770.1880.1340.1040.0850.0720.1810.1550.1280.1180.1010.365

40.2560.1680.1310.1080.0870.0750.2220.1840.1590.1310.1090.384

30.2980.2030.1480.1170.0960.0820.2030.1810.1520.1280.1090.548

20.2770.1940.150.1160.0990.0850.2520.2190.180.1430.1160.655

10.330.2070.160.1380.1220.1080.2940.2560.2060.1590.1290.71

PY79

20-SepHT251

oral0.8050.7360.7090.6210.5210.3210.4580.390.3290.2740.1960.05

0.8010.7720.7220.6190.4990.3570.2980.2730.2460.1650.1310.054

0.7340.6360.5360.3920.2630.1680.3070.2570.2010.1690.1210.416

0.6750.6080.4990.4010.2580.190.3320.2510.2310.1730.130.435

0.3980.3670.3060.2480.1860.1130.3530.2840.2240.2080.1550.246

0.8310.8060.7880.7340.6410.4990.3840.3410.2830.2050.1590.363

0.6350.5220.4090.290.1980.1350.3240.2830.2330.1780.1450.469

0.7930.7120.6180.5470.4960.3530.3610.2930.2660.2010.1410.589

IP251-ipMIX

17-80.3490.3070.240.1830.1420.1060.2960.250.2090.1590.1190.053

17-70.3650.3020.260.210.1550.1130.330.2770.2450.1970.1520.046

17-60.2640.2490.210.1660.1240.0950.2880.2260.1890.1460.1040.476

17-10.3090.2830.2530.1990.1490.1140.3850.3280.2720.2160.1580.502

16-80.2880.2610.2260.1860.1430.110.3260.2570.2340.1890.1380.263

16-70.2820.2560.2270.1880.140.1090.3630.3110.2650.2280.1590.392

16-40.3350.2920.2420.1940.1410.1070.3640.3090.2810.2380.180.558

16-10.360.310.2440.1950.1480.1080.3260.2960.2470.1960.1550.638

230-ippy-ip

ipPy+propro-only

15-80.3190.3010.2390.1860.1340.1020.3910.3370.2720.2030.1340.045

15-70.3060.2770.2410.1870.1430.1020.3520.30.2660.1940.1470.049

15-60.3310.3080.2440.1730.1270.1010.4030.3680.3140.2480.1510.485

15-50.3970.3520.2930.2020.1560.1080.4070.350.3080.2410.1650.497

15-40.3310.2870.2280.180.1280.0930.3050.2630.2320.1780.1280.204

15-30.3780.3460.2770.2050.1520.1070.3140.2540.2270.1770.130.341

15-20.4230.370.3090.2340.1710.120.4630.3610.30.2280.1690.46

15-10.420.3740.2870.2220.1550.1120.4170.3150.2780.1980.1380.585

21-SepHT230Nasaloral

0.7970.6070.390.2560.1850.120.340.1990.1430.1120.0880.043

0.3850.2150.1340.0980.0750.0590.3340.1860.1340.0960.0730.033

0.7120.4390.2680.1730.110.0750.2880.190.1140.0940.0750.134pool

0.5690.3420.2120.120.0840.0570.2460.1390.1070.0940.070.128

0.6160.4240.2490.160.1030.070.1640.0870.070.070.0450.292

0.490.2780.1720.1160.0810.0550.2530.1130.0690.0740.0560.5

0.6940.4250.2410.1580.0990.0740.3110.2110.140.1080.0810.57

0.6620.4090.2440.170.1230.0870.3380.1970.1320.1030.0870.729

IgA

HT251Nasaloral

0.9670.8870.7720.6070.4290.2710.960.9250.8830.8140.710.026

0.8660.6880.4540.280.1820.1140.930.8570.8130.7380.6270.033

0.7340.5260.3250.2110.1380.0910.6370.4460.2820.1930.1440.124pool

0.5740.2990.1740.1080.0860.060.6360.440.2740.1630.110.121

0.9220.7950.5890.3930.2450.1470.1520.0990.0740.0560.060.376

0.8990.7460.4820.2490.1530.0910.9420.9350.8540.8030.6930.531

1.051.0230.9660.9520.8880.7830.5790.3590.2040.1390.0980.653

0.860.6720.460.320.220.1360.9070.8880.8030.7010.6590.691

HT266Nasaloral

0.4990.2860.1680.1110.0750.0560.8020.7150.5780.5210.4650.044

0.7390.6170.4580.3290.2380.1390.5290.390.2460.1630.130.024

0.5780.4040.2440.1390.0850.0620.5550.2530.1510.2080.090.176pool

0.7140.5960.4190.2810.1890.1440.2710.1850.1160.0940.0640.168

0.8240.6120.3610.280.1770.0950.3430.2160.1120.0880.0610.381

*0.390.2140.1410.1010.0690.0470.3960.2610.1890.1280.0840.522*

0.2120.1490.0890.0660.0530.0390.4150.1870.1230.0850.0620.549

0.3890.2290.1280.0810.0670.0360.2850.2070.1140.090.0450.538

HT230Nasaloral

0.8060.620.4020.2640.1920.1240.3470.2030.1460.1160.0930.047

0.3880.2190.1390.1020.0770.0610.3440.1890.1350.0990.0750.035

0.7230.4540.280.1810.1150.0770.2950.1920.1130.0910.0770.142pool

0.5820.3550.2220.1260.0880.0590.2540.1420.1090.0940.0720.139

0.6270.4370.2570.1660.1070.0710.1660.0890.0710.0710.0470.308

0.4950.2860.1780.120.0830.0560.2570.1150.0710.0760.0580.526

0.7020.4370.2470.1630.1040.0770.3230.2170.1430.110.0850.611

0.6660.4170.2520.1750.1290.0920.3490.2060.1390.1080.0940.788

3-Octrepeat IgA4080160320640

9-10.8730.7470.570.3860.2570.1570.960.9220.8590.7710.6140.8432-8

9-20.5740.3730.2490.1610.1150.0890.9680.9310.8830.80.6670.635-1

9-30.7150.4970.330.210.1450.0920.2340.1750.1340.1080.0880.4235-4

9-40.8250.6710.5120.3480.2440.1610.6820.4880.3460.2410.1740.2515-5

9-50.3660.2380.1690.1220.0960.0750.2990.2090.1570.1190.0910.2826-1

11-80.8810.7880.660.5090.3470.210.4630.3190.2330.1690.1290.2546-3

12-20.050.0360.0250.020.0150.010.0940.0520.0390.0330.0270.0056-5

12-80.4870.2960.1850.1260.0820.0620.9250.8150.7470.6080.4340.0356-8

40801603206401280

Saliva-IgA

24-Sep

py-1

0.1680.130.1070.0880.080.0710.130.0870.0750.0690.0550.047

0.030.0460.0540.0580.0630.0610.0450.0520.0610.0630.0480.04

0.0730.0790.0810.0720.070.0690.0310.0460.0510.060.0470.189

10-20.0360.0480.0520.0520.0610.060.0290.0380.0440.0490.0390.264

0.0790.080.0740.0670.0680.0630.0670.0770.0740.0590.0460.381

0.0170.0250.0370.0420.0540.0540.0320.0570.0560.0520.0440.552

0.0440.0640.0630.0560.0650.0560.0710.0790.0740.0620.0480.674

10-10.0170.0240.0270.0330.0490.0520.0240.0350.0440.0360.0390.746

10-3

10-Oct

251-oralpre1st2ndfinal

10.0390.0610.0580.060.0540.0510.0530.0620.0620.0610.0630.065

20.0190.0260.0290.0320.0330.0360.0430.1040.0890.0740.0680.069

30.0380.0220.0270.3240.1880.1320.1010.9670.9010.7020.5410.367

40.0220.0180.0220.0210.0210.0250.0360.0390.0440.0460.0510.057

50.0250.0070.0250.0230.0260.030.0390.0220.0290.0290.0370.05

60.0210.1010.080.0320.0360.040.050.2380.2240.1760.1390.113

70.0260.0650.0610.0510.0520.0490.0550.470.450.3230.2660.062

80.0190.2430.1870.050.050.0530.0581.03910.8620.7150.075blank

average0.067875

251-nasalpre1st2ndblankfinal

10.0580.0440.0340.050.0540.0520.0620.3070.2120.1740.1480.119

20.0720.0430.0420.6920.530.3840.3180.8420.7840.6240.4870.311

30.0910.0230.0250.040.0330.0310.0370.8020.6850.5190.3650.272

40.0350.0610.0550.1110.0830.0720.0690.4560.3260.2250.1480.109

50.0440.0390.0370.0480.0410.0350.0390.2070.1730.1370.1010.106

60.0520.030.0270.1360.0980.0710.0570.6160.4880.4470.30.249

70.050.0450.0450.1230.0920.0740.0650.5540.4410.3130.2470.182

80.140.0650.0610.1450.1230.10.0760.6080.5190.4280.2790.21

0.04375

266-oralpre1st2ndfinal

10.0310.0220.020.0540.0510.0520.0560.0320.0370.0380.0450.052

20.0250.0270.0290.0370.0380.0420.0480.0790.0690.0630.060.062

30.0860.0610.0550.0750.070.0670.060.0670.0750.0630.0570.057

40.0260.0280.030.0650.0670.0650.0610.0520.0480.0440.0480.053

50.0390.0370.0370.0330.030.0310.0390.0380.0430.0450.0460.053

60.0240.0420.0420.0460.0470.0510.0530.0460.0480.0520.0580.062

70.0410.0540.0460.0280.0280.030.0350.0660.0570.0590.0540.059

80.0350.030.0590.030.0320.0390.0450.0740.0740.070.070.068

0.037625blank

266-nasalpre1st2ndfinal

10.2430.0750.0640.0760.0770.0710.0730.2070.1290.1110.0960.095

20.0760.0530.0540.0840.0890.0820.0820.8060.6260.5030.3680.285

30.2560.060.0550.0880.0760.0750.0720.380.2390.1790.1330.122

40.1090.0410.0430.0910.0660.060.0680.6950.6190.4420.3330.255

50.2460.0430.0420.0890.0740.0650.0680.510.3560.2730.1960.159

60.070.0390.0390.0330.0330.0340.0440.1540.1250.1150.1040.103

70.0570.0690.0530.0640.0540.0550.0580.1130.0970.0880.090.093

80.2920.0950.0610.0990.0870.0780.0770.2270.1570.1190.110.099

0.059375blank

ht230-oralpre1st2ndfinal

10.0350.0490.0490.0520.0560.0640.0550.0920.0970.1060.0970.088

20.0720.0830.0670.0560.0570.060.0590.0330.0390.0360.0410.049

30.0270.0260.0250.1260.1090.0840.0740.2390.1820.1380.1050.09

40.0930.0640.0580.0460.0420.0440.0460.0640.0610.0550.0590.059

50.0350.0510.0470.0550.0470.0510.0530.0760.0730.0710.0740.068

60.0350.030.030.0480.0470.0480.050.1350.1210.1030.0940.081

70.0450.1820.0610.0560.0620.0740.0640.150.1560.1340.1120.091

80.1030.1130.0670.0390.0450.0650.0510.0560.0530.0520.0550.059

0.07475blank

ht230-nasalpre1st2ndfinal

10.0980.140.1320.0690.0870.110.0820.080.060.0570.0640.089

20.1130.1420.1220.1160.1160.0970.0810.1090.0890.0860.0780.091

30.0940.1310.130.2120.190.1270.0970.0690.0630.0650.0640.085

40.2470.1280.1390.0550.0650.060.0540.2450.1960.1870.1610.138

50.1550.0510.0460.1180.1120.0880.0740.1050.0940.0880.0860.099

60.3340.1830.1590.030.0330.0360.040.1490.1270.1140.110.107

70.070.1950.060.0790.0710.0730.0660.0860.0850.0820.0760.094

80.1080.1590.0830.0870.0840.0930.0860.3310.30.2490.2210.188

0.141125blank

11-Oct

oralprefinalpro-onlyprepy+pro

0.0790.0690.090.0770.0670.070.1260.1080.0780.060.0740.085

0.0710.0550.0420.0380.0310.0490.0290.1090.0860.0590.0580.084

0.0760.0550.0510.0390.040.0440.0310.0620.050.0410.0510.078

0.0680.0560.0550.0870.0730.0590.0550.2920.2030.120.1040.115

0.0460.0530.0340.0280.0280.0460.0360.0450.0420.0480.0490.078

0.1110.0480.1120.0690.0580.060.0380.0520.0550.0520.0550.085

0.110.050.0310.0280.030.0340.0390.6030.4040.2890.1950.165

0.0770.0590.3220.1990.1250.1010.0490.7420.550.3620.2680.214

blank40801603204080160320

nasalprefinalpro-onlyprepy+pro

0.0610.0550.1530.1240.0950.0770.050.4950.3270.2250.1650.136

0.0580.040.0950.0770.0680.0610.0440.250.1770.1320.1070.089

0.0580.0510.0460.0440.0430.0420.0220.1580.1250.1010.0810.074

0.0570.050.1090.0950.0830.070.0730.8540.7280.5940.4810.381

0.0570.0470.0710.0640.0620.0580.0350.0690.0590.060.0540.06

0.0570.0270.0830.0680.0610.0570.0580.4940.3550.2570.1910.142

0.0550.0430.0690.0630.0580.0520.0160.9130.8290.6580.5120.351

0.0550.0440.0370.0360.0370.0410.0610.1560.1290.1220.0950.08

blank40801603204080160320

IPprefinalprefinal

266-10.0910.1170.1030.0910.0830.10.0970.0790.0750.0780.0930.102

266-20.090.0880.080.0830.0750.0950.0950.0810.0780.0720.0790.099

266-30.0610.0750.0580.050.0380.0510.0960.0530.0550.0570.0720.082

266-40.080.0840.0760.0670.0560.0560.240.1040.0910.0790.0740.082

266-50.0740.0950.0870.0780.0590.0610.0850.070.0690.0650.060.08

266-70.070.0780.0750.0670.0560.0640.1080.130.0910.0850.0680.067

266-80.0640.1010.0850.0780.0770.0920.0740.1240.1050.0860.0750.071

Blank0.0740.070.0880.0930.090.110.3480.0830.0790.0940.0960.111

IPprepro-IPprepy+problank

10.0650.0690.0630.0780.0850.0730.0490.0580.0730.0910.0840.105

20.0670.0730.0780.0760.0810.0380.0960.0810.1090.1040.0980.102

30.0870.0380.0450.0510.0570.0460.0960.0650.0860.0960.0770.09

40.1170.0330.0360.040.0480.0680.0450.0460.0650.0760.0710.094

50.001000.0010.0020.0020.002000.00100.001

60.1540.0420.0480.050.0590.0960.0940.0710.0890.0950.0820.09

70.0930.0790.070.0660.090.0910.0680.0570.0650.0960.0720.112

80.080.1160.0950.0820.0930.0760.1080.0770.0870.0880.0990.106

prefinalprefinalprefinalnaive1naive2

0.0720.1360.1060.0230.0840.0650.0960.0590.0570.0950.1210.067

0.0870.0830.0820.0460.1760.1310.0970.110.0930.040.0630.07

0.0350.0930.0890.0140.0690.0570.0350.0560.0540.0590.0590.063

0.0350.1080.1130.0570.1020.0780.0460.0610.0570.0660.060.065

0.0570.0920.0910.0280.0680.050.0260.0960.0840.0580.0590.064

0.0310.0750.0670.0420.0590.0480.0460.0780.0680.0660.0580.065

0.0460.0460.0420.0380.1080.0720.0680.0990.1090.0610.0590.063

0.0180.0740.0760.0570.1070.0730.1110.0560.0540.0580.0580.063

py-oralpy-nasalpy-ip

IgA-feces

8-Oct

230oralnasal

10.1090.0690.070.0560.0540.050.0880.0680.0610.0580.0520.761

20.0810.0660.0740.0640.0550.0510.2540.1850.1120.0830.0620.598

30.1140.0710.0660.0570.050.0450.1620.1140.0930.0710.060.447

40.1540.1080.0970.0750.0610.0540.4030.2230.1490.1010.070.298

50.1870.1480.1530.1380.0960.0850.1750.1080.090.0740.0620.045

60.1490.090.0870.0760.0610.0550.1990.1360.1060.0820.0680.049

70.2550.1930.180.1290.0890.0780.3350.2170.1740.1280.0910.17

80.2770.160.1260.0910.0710.0650.7260.5760.3810.2340.140.15pool

251nasaloralpos

10.6750.4530.3430.2290.1430.1030.1950.1270.1060.0830.0770.761

20.8180.660.5530.3780.2370.140.870.8510.8550.7560.6820.601

30.5450.2750.2190.1360.0880.0630.620.4640.3810.2580.170.431

40.440.2370.1850.1180.0710.0630.1530.0830.0760.0650.0530.303

50.5580.3470.2480.1460.0890.0690.1220.1450.1370.10.0730.052

60.5590.3430.280.1710.0990.0740.1050.0730.0710.060.0540.056

70.8680.6340.6060.4080.230.1450.5310.3330.2190.1390.0950.192pool

80.590.3740.3040.2110.1140.0920.7990.6050.4660.2640.1940.175

266oralnasalpos

10.2960.220.1530.1210.0930.0780.7250.5240.3820.270.1820.835

20.3130.2260.1580.1160.0840.0690.4860.3650.2630.1880.1410.69

30.1160.1080.0870.0740.0640.0560.7170.5720.4250.2740.1720.519

40.1530.0920.0860.0740.0650.0590.8790.7530.6360.4560.3070.377

50.4240.2180.2030.160.1240.1041.1890.9310.770.5020.3070.114

60.2190.1210.1090.0890.0690.0620.8590.7230.5790.3990.2520.056

70.2170.1280.1110.0910.0720.0630.450.3250.2430.1810.1340.13

80.5140.2920.2370.160.1110.0860.5010.3620.2540.1850.1340.167

266oralnasalpos

10.2960.220.1530.1210.0930.0780.5010.3620.2540.1850.1340.835

20.3130.2260.1580.1160.0840.0690.4860.3650.2630.1880.1410.69

30.1160.1080.0870.0740.0640.0560.7170.5720.4250.2740.1720.519

40.1530.0920.0860.0740.0650.0590.8790.7530.6360.4560.3070.377

50.4240.2180.2030.160.1240.1041.1890.9310.770.5020.3070.114

60.2190.1210.1090.0890.0690.0620.8590.7230.5790.3990.2520.056

70.2170.1280.1110.0910.0720.0630.450.3250.2430.1810.1340.13

80.5140.2920.2370.160.1110.0860.7250.5240.3820.270.1820.167

9-Oct

Py+pro-oralpro-oralpos

0.3160.2120.1470.1160.0980.0850.2670.1670.1140.0960.0760.757

0.3040.2310.1750.1330.1060.0860.1660.1240.1030.0860.0690.581

0.3050.1660.1070.0760.0620.0530.210.1870.1490.1030.0730.37

0.6180.5090.3840.2510.1860.1210.3350.2510.1880.1320.1130.231

0.4730.3430.2830.2180.1580.1090.1680.1530.1250.0890.0730.046

0.0750.0620.080.0790.0720.0690.2990.190.1270.0950.0770.055

0.4820.3750.3050.2330.1620.1410.2050.1440.1180.0910.0740.292

0.8020.6710.5470.3740.2310.160.3540.270.2110.1560.1240.308

Py+pro-nasalpro-nasalpos

0.2290.2090.1920.1480.1380.1090.4380.3190.2510.2110.1820.879

0.7130.5450.4280.2910.2180.1670.4520.2850.2380.1720.1430.723

0.3880.2840.2250.1640.1260.1140.3730.2820.2320.1880.1470.55

0.6010.4290.3130.1990.1370.10.820.6680.4830.2890.1920.377

0.640.4330.3710.2740.1970.1530.1490.2710.3510.2940.2340.083

0.5260.4110.2910.2040.1480.1350.1960.1690.1870.1660.1350.089

0.8060.7110.5380.3640.2330.1680.2460.1960.1660.1430.1120.362

0.6310.5680.4830.4110.3220.2390.2490.2190.2070.1620.1440.374

Py-oralPy-nasalpos

0.3870.3870.3640.2790.2090.1650.3720.2490.2210.1650.1340.693

0.2650.150.1540.1540.1320.1080.2540.2010.1760.1650.1240.592

0.3210.2380.2020.1730.1410.1260.4760.2960.2380.1960.1420.39

0.4450.2370.1710.1370.1210.1110.4280.2550.1940.1420.1180.288

0.2360.1290.0990.1050.1130.0850.2630.1560.1190.10.0920.07

0.3480.1970.1280.1340.1050.0910.2410.1780.1320.1160.1020.082

0.2910.1730.1140.0980.090.080.2140.1620.1280.1170.0960.312

0.160.120.1130.0980.0970.0890.4080.2320.2170.1690.1390.326

HT266-ippos

4-10.1290.0950.0960.0850.0810.0770.8210.7080.5530.4340.3230.68

4-80.260.2010.190.1170.1040.0720.5590.4350.3160.2040.140.552

5-30.7680.5730.4150.270.2020.150.4650.310.2440.1760.1750.393

5-40.2250.1710.1380.1080.0950.0880.4290.3240.2470.1850.1650.306

6-80.4780.3130.2270.1560.1080.0930.3990.2020.1350.1060.1070.065

12-70.3580.2810.2080.1340.1280.0940.1320.2370.280.2280.2070.067

nave0.3970.2480.1750.1120.0980.0890.3350.210.210.1650.1520.237

py7-10.3210.2250.170.1180.1030.090.0920.080.0710.0620.0730.257

IPPYpos

17-10.0510.0390.0390.0360.0370.0540.3090.1530.1510.1030.0810.713

17-60.1410.1170.0980.0740.0760.0990.2040.1570.140.1190.1020.607

17-70.0590.0520.0410.0390.0540.0870.4650.2530.1870.1370.1080.461

17-80.0590.0430.0340.0290.0390.0530.2150.1350.1060.0780.0650.334

16-10.5410.4330.2640.1620.1380.0930.2480.160.1380.1040.0720.037

16-40.3970.2490.1730.1150.1090.0840.3680.2260.1650.1140.0840.039

16-70.4730.420.3510.2690.2620.2090.4840.3240.2410.1760.1520.271

16-80.2540.1540.1230.1020.0890.0780.0430.0430.0420.0410.0510.238

final-IgG

25-SepTOTAL IgG

plate1HT230-ORALpy-oral

0.0730.0440.0320.0310.030.030.0810.040.0350.0290.030.034

0.0990.0420.030.0270.0260.0240.0790.0430.0350.0270.0290.028

0.3120.0940.0570.0430.0310.0280.1070.0540.0410.0280.0260.105

0.0890.0410.0340.0320.030.0250.0870.0470.0370.0250.0260.1

0.0860.0420.0320.0290.0280.0250.1140.0440.0340.0320.0240.708

0.0930.0390.030.0270.0270.0260.0960.040.0330.0290.030.808

0.0950.0390.0340.0330.0280.0290.0760.0450.0340.030.030.776

0.2690.0650.0470.0340.0290.0250.1330.050.0370.030.0340.848

plate2HT251-ORALprotein-oral

0.7250.670.6430.6330.6110.5910.6340.5840.5470.4250.2660.026

0.7120.6550.6150.5920.5550.490.0680.0480.0340.0270.0240.02

0.5370.4570.3690.2650.1730.1030.0570.0310.0260.0210.0190.069

0.1070.0450.0320.0260.0250.0220.0610.0340.0270.0240.0180.074

0.0890.0320.0250.0210.0190.0220.0620.0320.0230.0190.0160.6016400

0.7120.6660.6240.5980.5660.5520.0650.0340.0260.0190.0170.5943200

0.3740.1370.0750.0450.0320.0250.0520.0290.0210.0180.0140.5861600

0.6810.6380.6110.5770.5480.5080.0460.0240.020.0170.0150.663800

10020040080016003200

Plate3ht266-nsalht266-oral

0.3580.1810.1110.0620.0410.0330.4990.460.4070.3220.2220.025

0.5410.5090.4440.4140.3210.1860.5430.4720.3770.2610.1550.019

0.3590.160.0910.050.0340.0220.2510.0780.0420.0250.0190.069

0.6120.5630.5240.4680.4080.3430.2160.0860.0450.0330.020.077

0.5890.5260.4830.4080.3140.2230.4160.3760.3440.3260.2560.539

0.5570.450.3940.2590.1430.0990.3040.1310.0820.0460.030.533

0.4730.2440.1410.0810.0430.0260.040.0170.0130.0130.0110.442

0.5570.40.2820.1580.0980.0490.0640.0290.0230.0160.0130.514

plate4ht251-oralnasal230-nasl

0.5990.5540.5190.4730.4010.3050.4960.3230.1990.1130.0630.02

0.5080.4570.4020.2990.1980.110.3980.1710.0870.050.030.018

0.540.4810.4410.3780.2880.1910.4220.2380.140.080.0460.058

0.4830.3220.2060.1190.0740.0440.5370.4360.3260.1980.1090.059

0.5920.5350.4950.4340.3390.2320.5030.4130.3050.190.1050.525

0.5690.5080.4220.3170.1910.1110.3510.1480.0830.050.0290.566

0.6120.5530.5110.4620.3880.2780.3410.1340.0740.040.0260.567

0.5370.4150.3050.1920.1150.0640.4840.3190.1940.1110.0590.633

plate5266-ip251-ip

0.0690.030.0340.0240.0240.0180.580.4790.390.3360.2280.022

0.5860.3470.2630.1340.0860.0490.3310.1630.0940.0520.0350.023

0.0920.0350.0280.0270.020.0220.5550.4480.2450.1930.150.05

0.4810.230.1590.0860.0620.0450.4190.2480.1420.0760.0490.05

0.6010.4620.3550.2320.1390.0840.0590.0290.0210.0270.0170.569

0.3290.1040.0680.0460.0340.0250.1490.0480.030.0270.020.573

0.0990.0360.0290.0220.0210.0190.3350.1510.0820.0440.0290.604

0.4370.2130.1480.0650.0440.020.0470.0280.0220.0170.0180.662

230-ip

plate6pro-nasalpro-oral

0.0670.03-0.0140.0160.0140.0140.5980.5640.5410.4750.5090.014

0.0410.022-0.0130.0120.0170.0160.3730.2230.1420.0760.050.011

0.0460.023-0.010.0150.0150.0150.1840.0960.0440.0310.0250.076

0.0370.024-0.0080.0150.0150.0160.0180.0190.0160.0130.0150.061

0.1320.038-0.0170.020.0170.0180.4210.3990.2490.1860.1260.56

0.0350.024-0.0110.0150.0160.0160.2270.0630.0450.0280.0230.514

0.2350.084-0.0280.0280.0190.0160.0670.0180.0160.0140.0120.522

0.0580.021-0.0120.0120.0130.0120.0430.0170.0140.0120.0120.562

plate7py+pro nasalpynasal

0.2440.1310.10.0520.0430.0290.0990.0370.0330.0280.0230.028

0.350.150.0820.050.0320.020.0430.030.0140.0280.0130.016

0.620.5140.4680.3290.2070.1190.0390.0290.0270.0190.0160.059

0.3840.1770.1120.0580.0410.0290.0610.0320.0210.0160.0210.054

0.6070.4920.490.4190.310.1650.0530.030.0240.0190.0190.532

0.5350.2960.1840.1260.0580.0370.0490.0270.020.0210.0190.591

0.5060.2230.1520.0940.0410.0260.0590.0250.0190.0150.0140.597

0.6690.5690.5230.4280.3080.1940.0580.0270.0230.0160.0140.646

27-Sep37C

pool0.3130.150.0950.0650.0470.0390.030.0280.0230.0220.020.029

py-20.3160.1120.0690.0490.0370.0310.0280.0220.0210.0210.0180.023

PY-10.2680.0950.0650.0410.0370.0320.0270.0240.0220.020.0210.022

251-3-oral0.80.7720.7890.7970.7730.7260.6770.5660.4560.2780.0170.021

251-20.550.2250.1330.0870.0550.0380.030.0220.0210.0190.0160.02

251-10.820.8390.8140.7670.7590.7250.6390.5220.3710.2270.0190.022

ip-new0.8340.8310.8140.7620.7550.6920.6060.4560.320.1860.0220.019

ip-old0.8130.7840.7220.6930.640.5640.4280.2750.1690.1010.0180.018

20004000800016000320006400012800026400

4C

pool0.2320.1460.0920.0640.0450.0350.0280.0270.0240.021-0.015-0.01

py-20.220.1140.0730.0490.0330.030.0230.0230.020.019-0.009-0.008

PY-10.1920.0950.060.0410.0310.0280.0230.0210.020.023-0.01-0.009

251-3-oral0.7240.6790.6710.620.5780.5710.5460.4760.4160.309-0.011-0.009

251-20.5310.2750.1580.0890.0490.0340.0220.020.0170.015-0.011-0.009

251-10.7410.720.7240.6850.6820.6670.6430.5330.4070.265-0.01-0.008

ip-new0.7890.8040.7820.6770.6510.6110.560.4550.2830.183-0.011-0.01

ip-old0.7080.710.6310.5890.5270.480.3780.2650.1560.099-0.012-0.01

20004000800016000320006400012800026400

dilutionbuffer:BSA0.2%

no tween

28-Sep1hour

pool0.3560.1290.0690.0350.0250.0170.0140.0120.0120.010.0090.011

ip1.0851.050.9890.9270.8110.6690.4970.3020.1640.0870.0480.01

251-3-O1.0811.0741.0110.9880.8940.7390.6010.4270.2370.140.0720.011

PY-1-N0.2380.0870.0560.0340.0210.0180.0150.0130.0070.0080.0080.009

PY-N-F0.1780.090.0570.0420.0330.030.0270.0220.0210.020.0220.028

Lung-12-40.4940.3530.2250.1370.0870.0580.0410.030.0280.0260.0240.017

251-3-O-F0.890.7820.6540.4690.280.1560.0910.0560.0380.030.0280.025

251-3-N-F0.3720.2220.1520.1080.070.0490.0380.0310.0340.0290.030.03

2hour

pool0.260.1180.0620.0310.0210.0170.0160.0160.0160.0170.0110.008

ip0.8540.810.7930.7370.6690.5890.4720.3260.20.110.0610.007

251-3-O0.8820.8670.8370.7980.7160.690.5880.4210.3050.1670.0920.007

PY-1-N0.2240.1030.0740.0420.0230.0170.0120.0080.0080.0070.0060.008

PY-N-F0.2450.1120.0730.0470.0380.030.0280.0210.0180.0180.020.02

Lung-12-40.4970.4120.2830.1810.1010.0640.0450.0340.0240.0210.0190.02

251-3-O-F0.7680.7140.7150.490.3110.190.1150.0660.0420.0290.0260.02

251-3-N-F0.340.2340.1820.1130.0770.0560.0440.0230.0270.0250.0230.023

1-Oct

pool0.0390.0460.040.0420.0460.040.0380.050.0340.0290.0360.06

Nave0.1710.1230.10.0770.0720.0550.0430.0460.0380.0460.0430.065

PY-20.0410.0470.0430.0410.0380.0390.040.0420.0450.0390.0410.039

PY-10.1620.130.1040.0790.0620.0520.0430.050.0450.0470.0360.042overnight incubation 1st ab

5-30.5830.5810.5940.6080.6580.6960.7210.530.2990.1830.0750.046

5-20.3550.2630.1980.1230.090.080.0560.0510.0470.0480.050.057

5-10.6070.570.5780.6450.6690.6530.5340.360.1810.1250.0760.052

IP0.4440.4450.460.4480.4820.4930.5990.4260.2210.1370.0890.055

1-Oct

251-oralIP

10.8210.8120.8120.7410.7330.6580.6650.6770.5040.3370.8010.007

20.5120.2720.1720.10.0580.040.0230.0160.010.0090.7150.011

30.8660.8530.8940.8370.8340.8140.7580.6260.5520.40.7820.006

40.1230.0440.0220.0140.0120.0140.010.0090.0050.0040.6630.006

50.2090.0760.0470.0280.020.0160.0110.0060.0050.0040.5750.005

60.6820.6590.4640.380.2880.1470.080.0460.0240.0130.4370.008

70.7930.7910.80.7450.7120.6190.5670.4780.3350.1710.2760.11pool-py

80.8350.7930.8290.7770.7350.670.6860.6740.4930.4010.1590.121

5010020040080016003200

251-nasalIP

10.7220.6650.5650.4060.2470.1390.0730.0370.0190.0140.890.017

20.7760.7970.7720.730.6860.5780.450.2750.1380.070.8550.007

30.7390.7370.6960.5960.4190.2710.1420.0730.0340.0180.8030.005

40.7690.7780.7490.7020.5830.4860.330.1670.0770.0450.7380.008

50.7270.5850.430.2810.1460.0860.0470.0230.0110.0050.6170.006

60.80.7240.6940.6520.5090.3980.2550.1360.0630.0340.4490.009

70.7870.7290.6230.5390.370.1980.1080.0560.0240.0150.2760.117

80.8480.8110.8090.7420.670.550.4080.2560.130.0760.1680.123pool-py

5010020040080016003200

266-oral5010020040080016003200IP

10.0810.0360.0260.020.0140.010.0080.0090.0070.0080.6090.011

20.050.0180.0120.0090.0060.0040.0030.0050.0060.0040.6020.005

30.3410.1850.1070.060.0310.0160.0140.0060.0050.0030.5840.005

40.540.5050.4490.420.3540.260.1730.0950.0550.0250.5490.005

50.3780.1650.0980.0540.030.0190.0140.0060.0030.0040.4910.004

60.2670.1210.0680.0360.0210.0110.0080.0060.0040.0040.3580.005

70.7250.6820.6330.5760.4980.2960.1930.0890.0420.0220.2120.09

80.7330.6790.620.570.4680.3150.1850.0970.050.0220.1350.094pool-py

5010020040080016003200

266-nasalIP

10.4730.3560.2780.1720.1020.0610.0380.0170.0080.0050.4520.002

20.4390.3320.2120.1220.0820.0410.0230.0120.0060.0060.5590.001

30.50.5050.5110.4060.2770.2310.1210.0510.0230.0120.5190.003

40.630.5910.5470.4960.4490.3520.2610.1130.0570.0210.4850.003

50.6250.5750.5190.510.4960.3810.270.160.080.0450.4090.002

60.5120.3110.1890.1130.0530.0330.0170.0080.0070.0050.3170.004

70.6320.560.5160.4250.3770.2830.2030.080.0430.0220.1820.092pool-py

80.480.270.1430.0850.0470.0210.0150.0070.0040.0030.1170.086

5010020040080016003200

2-Oct

251-oralIP

11.0331.0581.0031.0121.0220.880.8020.6320.4130.2441.0890.009

20.6040.3520.2030.1120.0640.0370.0250.0190.0140.0131.0240.006

31.0661.0621.0861.0430.9820.9460.8820.7510.5310.3840.9930.008

40.1220.0560.030.0210.0180.0150.0140.0130.0090.0090.9020.008

50.2380.1080.0630.0340.0210.0150.0140.0110.0110.0080.8220.005

60.7620.7230.5780.3960.2560.1360.0780.0430.0270.0170.6560.11

70.9931.0411.0251.0090.9520.8740.7310.5480.3090.1650.4620.103pool-py

80.9871.0731.0281.0161.010.8960.8180.6610.4830.3250.2510.1030.3

20040080016003200640012400

251-nasalIP

10.8670.80.6910.4960.3010.1680.090.050.0320.0541.0990.031

21.0150.9880.9860.9110.8060.6830.4570.2790.1450.091.0480.023

30.9870.9350.90.7670.5660.3670.1980.110.0570.0371.0060.018

40.9490.9380.8370.8390.7020.5170.3120.1680.0920.0510.9190.018

50.7890.7270.5760.380.2380.1150.0650.0370.0210.0150.7770.014

60.8540.8830.8530.7730.6750.4330.2720.1480.0720.0440.6570.106pool-py

70.8540.8340.7380.5820.3850.2260.1190.0660.0350.0220.4410.103

80.8980.9920.9460.8810.8650.6370.480.2770.1450.090.2430.12

20040080016003200640012400

266-oral5010020040080016003200IP

10.0960.0570.0350.0280.0260.0210.0190.0180.0160.0150.9570.017

20.090.0490.0320.0250.0230.020.0190.0170.0150.0160.8980.016

30.7180.5690.3660.2330.1390.0730.0480.0340.0290.0230.8620.019

40.840.7950.730.6390.5120.3030.1810.0970.0560.0360.7630.019

50.4210.2540.1440.0810.0470.0290.0220.0170.0130.0110.7510.016

60.4370.2490.1180.0650.0440.0250.0150.0150.0130.0110.520.078pool-py

70.8390.8620.7230.50.360.1730.0930.0530.0270.0210.4070.096

80.8560.860.7440.6180.4920.2590.1550.0790.0410.0270.2160.086

20040080016003200640012400

266-nasalIP

10.8070.7330.5250.3250.210.1050.0630.040.0280.0271.0070.017

20.6970.5440.3470.2190.1290.0680.0440.0410.0220.0191.0470.017

30.7470.6650.5140.3380.2240.1150.0680.0410.0280.0260.9550.02

40.9520.9540.8390.8550.7390.5030.3190.1790.1050.0590.940.015

50.9791.0450.9020.9250.870.6790.4930.3080.1660.0930.7130.01

60.4820.3230.1820.0980.0610.0330.0230.0170.0130.0120.5920.094

70.9190.9060.8820.7930.6740.5130.3140.1920.0950.0510.3660.0920.2

80.3660.2310.1240.0710.0410.0270.0170.0120.010.0090.2080.096pool-py

20040080016003200640012400

3-Oct

230oralIP

10.5340.3030.160.0870.0490.030.0210.0160.0140.0131.130.014

20.1620.10.0590.0340.0220.0150.0140.0130.0120.011.0730.009

30.150.0810.0430.0250.0190.0150.0140.0130.0140.0091.0310.01

40.1870.1360.1110.1060.0860.0760.0670.0620.0560.0420.9540.009

50.110.0590.0340.0230.0160.0140.0130.0120.0120.0080.8730.011

60.6190.3450.1740.1020.0590.0310.0230.0170.0150.0120.6870.012

70.140.0770.0440.0260.0190.0120.0120.010.010.0090.4680.094pool-py

80.1870.1320.0980.0680.0430.0280.0220.0170.0130.0120.2830.0940.2

20040080016003200640012400

230nasalIP

10.9650.8030.6260.4520.2770.1490.0850.050.0310.0221.2090.01

20.8130.5940.3640.2120.1180.0650.0380.0270.0190.0151.1670.01

30.7490.4930.2970.170.0910.060.0340.0230.0190.0141.1120.012

41.0420.9470.8380.7330.5410.3180.1850.1080.060.0351.0440.01

51.0460.9320.8140.6950.4880.2910.1610.0930.0570.0330.9180.011

60.9230.7890.5960.4060.2390.1260.0710.0430.0290.0210.7180.012

70.8950.6050.3870.2090.120.0620.040.0250.0190.0150.4750.094pool-py

80.9620.8360.7250.5390.3240.1840.1060.060.0380.0260.2780.1010.2

20040080016003200640012400

251-266 ipIP

17-11.0730.9490.7480.4770.2840.1530.0850.0530.0330.0231.2690.013

17-61.1781.0870.9910.8940.7180.4620.2640.1560.0910.0531.2270.012

17-70.9980.8130.520.340.1890.1040.0630.040.0250.0181.170.012

17-81.2451.1621.0821.0750.9870.8310.7280.5190.3350.1981.1130.012

18-10.9490.7560.4940.3240.1860.1010.060.0360.0250.0170.9970.013

18-20.2210.1270.0750.0440.0270.020.0160.0170.0140.0150.7840.013

18-30.8190.5650.3280.1850.0960.0550.0340.0240.0160.0150.5580.0790.2

18-41.1421.0510.8920.7610.5260.2970.1920.1110.0640.0410.3350.074pool-py

20040080016003200640012400

4-OctHT251-ORALIP

11.0651.0461.0280.9980.9260.8950.8110.6710.4910.2951.1070.031

20.6240.360.2030.1550.0650.0430.0290.0220.020.021.0460.021

31.0741.0511.0491.0180.9310.9160.8680.70.5550.3550.9790.021

40.1260.060.0370.0260.020.0180.0160.0140.0140.0170.890.018

50.2090.0980.0580.0360.0290.0190.0180.0390.0150.0150.7380.017

60.8870.7770.7390.5390.3420.1970.1120.060.0380.0290.5590.018

71.0711.0761.0480.9890.9060.8660.7690.5560.3660.2120.3390.093pool-py

81.0681.0641.0711.020.9970.9360.8860.7690.5930.4220.1920.1010.2

HT251-NASALIP

10.9030.7730.6850.4890.2810.1580.0860.0520.0310.0241.0670.03

20.9410.8630.9130.8050.6350.4750.2950.150.090.0551.0050.012

30.9310.8860.8320.6860.4660.2850.1680.0880.0560.0330.9610.013

40.9710.9120.9080.7940.6350.490.3260.1540.0980.0550.8930.012

50.8280.6630.520.3070.1680.0970.0520.0280.0250.0160.7670.01

60.9290.8660.9050.7670.6380.5190.3280.1640.10.0540.6330.012

70.8920.7950.7780.5780.3540.2110.1210.060.0380.0230.4440.069pool-py

81.0340.9620.9770.9340.8220.7350.6050.3670.230.1270.2660.0810.2

HT266-ORALIP

10.0740.0340.0250.0170.0140.0140.0110.0110.0090.0130.8260.024

20.0640.030.0220.0160.0120.0110.0120.010.0110.0190.910.016

30.6610.4290.3430.1750.080.060.0320.0180.0160.0150.8080.017

40.7570.7080.7210.6220.3860.2440.150.070.0470.0350.7340.014

50.4390.2220.1330.0710.0410.0230.0190.0130.0130.0120.60.014

60.4090.190.1230.0610.0320.0260.0180.0130.0130.0130.4480.015

70.8450.6870.6870.4610.2430.1270.0880.040.0250.0190.2650.077pool-py

80.9040.8110.8320.6460.4620.2940.1750.0850.0540.0270.1590.0780.2

HT266-NASALIP

10.9020.6630.6230.3840.2030.1290.0680.0360.0260.0250.9390.041

20.810.5760.440.2560.1260.0830.0420.0240.0190.0180.9150.018

30.7830.6750.6760.4450.210.140.0830.0380.0290.0220.8860.014

40.9280.860.9590.870.6520.5870.4130.2070.1130.0710.8020.014

51.0340.9620.9340.8440.7480.6280.4760.2650.1610.090.7890.013

60.6470.360.2370.1280.0590.0410.0270.0160.0160.0120.6020.014

70.930.8370.9240.8380.6310.5780.3670.1720.1090.0640.4160.069pool-py

80.4870.2660.160.0820.0430.0320.020.0140.0130.0120.2620.0790.2

IPIP

HT2300.1620.10.0740.050.0320.0240.0180.0170.0170.0181.0410.012

0.6740.4950.3580.2130.110.070.0420.0240.0210.0180.9910.013

0.3120.20.1340.0750.0420.030.020.0150.0170.0130.9810.014

0.1780.1120.0780.0560.0330.0210.0180.0180.0160.0150.8820.013

HT266 50.7790.5730.4250.2530.1360.080.0490.0280.0230.0190.8070.016

70.3710.2190.1420.0880.0470.030.0230.0170.0170.0160.6220.013

80.2040.1240.0870.0570.0340.0250.020.0150.0170.0170.4630.085pool-py

PY1-IP0.20.1630.1090.0750.0480.0330.0260.020.0190.0170.2830.0880.2

6-Oct

Py-oralpy-nasalIP

10.180.0940.0490.0310.0210.0170.1180.0610.0320.0240.8780.012

20.1150.0590.030.020.0130.010.1560.0820.0380.0220.8630.011

30.0830.0380.0210.0150.010.010.1240.060.030.0180.7890.01

40.1260.0680.0360.0220.0150.0110.1230.0660.0320.020.7440.01

50.1150.0570.0280.0190.0120.010.1120.050.0230.0160.6280.009

60.1490.080.0370.0260.0150.0120.1130.0550.0270.0160.4540.011

70.0890.0470.0220.0160.0110.0090.0630.030.0170.0130.2490.065pool-py

80.0860.0450.0230.0170.0120.0110.0920.0440.0190.0130.1350.0710.2

py+pro-oralpro-oralIP

10.1280.0710.0330.0240.0180.0140.10.0620.0320.0220.880.016

20.1430.0780.0330.0220.0150.0110.1350.0690.0490.0260.8620.013

30.3960.2260.110.0620.0330.0220.1730.0970.0410.0230.7550.012

40.1350.0710.0360.0220.0170.0110.1510.0850.0480.0230.6910.011

50.7080.7520.5950.4840.2810.1630.1380.0790.0360.0190.6150.009

60.4140.2430.120.0770.0380.0250.1180.0720.0330.0220.4730.012

70.6890.6620.4110.3250.1610.0940.1930.1150.0560.0290.2510.07pool-py

80.730.8360.8030.8080.7810.7080.8140.7810.7180.5550.1460.0830.2

py+pro-nasalpro-nasalIP

10.8470.7610.7060.7430.520.3220.1670.10.0440.0210.8440.011

20.6740.5140.3220.1860.0930.0390.3980.2230.1160.0570.850.009

30.670.5270.310.2060.0980.0470.1160.0640.0280.0170.720.011

40.7290.6830.6460.6390.4820.2410.1750.0760.0370.0270.6380.013

50.5430.3820.2660.1670.0920.0350.1050.0490.0210.0170.6330.01

60.7640.7460.6350.5810.4080.2050.1130.0550.0310.0190.460.012

70.4960.3330.1850.1190.0560.0250.1260.0690.030.0140.30.077pool-py

80.5540.4560.2660.1920.0910.0390.2330.1230.060.0320.1890.0760.2

Py+pro-IPPro-IPIP

0.3660.2070.1120.0610.0360.0260.1590.0850.0470.0250.8330.016

0.2220.1070.0560.0330.020.0150.1620.0930.050.0240.790.012

0.2780.1330.0550.0380.020.0150.150.0770.0390.020.7540.011

0.2550.1460.0580.0380.020.0140.1520.080.040.0190.6690.012

0.1490.0810.0330.0260.0150.0150.1130.0610.0340.0170.5390.01

0.2110.1180.0620.0380.0220.0150.010.10.0580.0270.4150.012

0.3060.1370.0690.0410.0210.0160.0990.0570.0340.0180.230.071pool-py

0.250.1370.0670.040.0240.0180.10.0610.0260.020.1410.0790.2

Py-ipIP

naive10.070.0380.0220.0170.0140.0130.3820.2640.190.0880.9310.012

naive20.0820.0410.0220.0170.0140.0120.2020.0870.0420.0250.860.011

2-3-10.1110.0540.0310.0230.0160.0150.2030.1020.0440.0260.8210.011

2-3-80.7650.7110.6630.5680.4320.2580.2270.090.0360.0220.7390.01

0.0110.0080.0080.010.0090.010.2830.1610.0660.0330.5380.009

0.0120.0080.0080.0120.0120.0120.190.0940.0420.0240.390.011

0.0140.0070.0070.0090.0090.0090.2290.1310.0560.0280.2320.064pool-py

0.010.0060.0060.010.0090.010.3310.20.0950.0450.1410.0620.2

12-Octht266

IP18-118-218-318-418-518-718-83-53-89-15-45-5

0.5180.1980.7250.9240.8190.4040.3570.8531.1131.0090.1340.155

0.3160.1050.4820.8810.6350.2540.2370.81.1280.950.0660.09

0.150.0650.2620.6960.4520.150.1310.6071.0850.8090.0430.062

0.0990.0350.1230.5450.2260.0790.070.3761.0210.6060.0330.043

0.0570.0250.0690.3150.1190.0450.040.210.9520.4490.0270.038

0.0460.0250.0450.1710.0760.0350.0320.1150.8170.2720.0270.038

0.030.0210.030.0880.0470.0260.0270.0650.6160.1430.0260.035

pos0.9360.8830.840.8480.6990.4410.2370.1280.0360.030.3430.326pool

ht251

ht23016-116-416-716-817-117-617-817-76-16-3

0.1120.5970.4150.2030.8891.0051.0870.8040.0740.0760.0250.029

0.0920.420.2170.1330.7960.9720.960.5860.0410.0390.0240.028

0.050.2170.110.0690.5990.8410.9570.3670.030.0290.0240.027

0.0290.120.0560.0360.3770.7260.810.2170.0260.0240.0220.024

0.0220.0610.0380.0280.2240.5390.6640.1310.0240.0210.020.024

0.0230.0430.030.0250.1130.3420.440.0760.0250.0190.0220.026

0.0180.0280.0240.0240.0620.1820.3070.0480.0230.0230.0220.024

pos0.9270.8960.9760.8980.6510.4990.3060.1550.0250.0240.2330.211pool

seroIgG-alpha

18-Octoral

4-14-24-3

400.3920.3640.4730.6950.2820.3210.5170.2370.380.3430.5320.302

800.2910.240.370.6020.2010.2270.2750.190.2790.240.420.19

1600.1730.1540.2550.4170.1270.1630.2560.1290.1830.150.2950.106

3200.0950.0920.1540.2460.0770.1030.1620.0850.1060.0960.1810.063

6400.0540.0570.0960.120.0480.070.1170.0470.0670.0590.1170.038

12800.0360.0380.060.0660.0350.0460.0320.0390.0490.0460.0740.028

25600.0250.0280.0420.0490.0270.0370.0550.0330.0330.0330.050.022

IP0.8430.8370.7960.7090.6040.3880.2590.1470.0170.0180.2280.224

8001600320064001280025600blankpool

4-44-54-6

400.3470.3490.5630.2350.3790.3640.5970.1850.5060.3610.6250.8

800.2850.2530.5280.1990.3540.2680.5520.1390.4060.2420.5330.71

1600.1710.150.3810.1310.1960.160.3680.080.2670.1350.3680.511

3200.1020.1040.3070.1010.130.0950.2530.0510.1580.0850.2450.28

6400.0640.0590.1810.0560.0690.0630.1540.0270.0910.0540.1470.138

12800.0420.040.1150.0360.0470.040.0950.0210.0590.0360.0850.08

25600.030.0280.0650.0260.030.0410.0580.0170.0380.0250.0560.048

IP0.8560.8210.7330.6710.60.3950.2320.1240.0110.0110.1950.203

8001600320064001280025600blankpool

4-74-81-1

400.3570.4740.6410.2860.5250.4550.6770.230.3620.4830.6110.254

800.2520.3410.5510.2240.3950.3450.5820.1620.2210.3360.490.152

1600.1370.1950.4020.1430.2630.1920.4540.0950.1240.1990.3180.094

3200.0840.1140.2650.0950.1580.1220.3260.0570.0780.1140.1960.05

6400.0510.0740.1820.0540.0890.0760.2120.0290.0490.0670.120.033

12800.0350.0440.1020.0390.0560.0480.1310.0220.0350.0450.0740.026

25600.0240.0290.0610.0250.0350.0320.0740.0160.0250.0290.0460.019

IP0.8310.8040.7910.6890.6270.3960.2830.1670.0120.0120.1960.2

8001600320064001280025600blankpool

5-15-25-3

400.4450.4080.8090.8710.4480.5290.6050.780.4090.4740.8480.976

800.3030.2320.7070.8620.310.3620.4580.6540.2560.3030.7490.953

1600.1740.1480.5890.8380.2030.2340.3270.4810.1570.1890.6260.957

3200.1040.0930.4070.7720.1210.1510.2120.2270.0950.1110.4410.903

6400.0650.0610.270.8320.0810.0960.1550.1610.0660.067.0.2760.885

12800.0470.0440.150.7840.0510.0630.0950.0970.0420.0440.1660.876

25600.0270.030.0870.7140.0320.0380.0570.0560.0270.0290.0840.829

IP0.8960.8290.8040.6620.5820.5820.230.1260.0150.010.1840.179

8001600320064001280025600blankpool

5-45-55-6

400.2680.3010.4560.2070.2560.3540.5230.9410.3430.3870.4950.942

800.2080.1930.3320.1120.170.2240.3760.8520.2260.2470.3750.849

1600.1030.1130.2160.0550.0970.1220.2490.8350.1380.1430.2450.767

3200.0780.0650.1360.0280.0620.0770.1630.7280.0780.0870.1550.669

6400.050.0450.0820.0220.0410.0510.0990.6370.050.0510.0910.531

12800.0360.0320.0540.0170.0280.0340.0590.4920.0350.0340.0580.36

25600.0260.0220.0350.0130.020.0220.0380.3370.0250.0220.0380.218

IP0.8860.7620.7950.6470.570.360.2150.1270.0150.0130.2050.209

8001600320064001280025600blankpool

5-75-81-2py-2251-8251-7

400.2290.260.7460.8910.2610.3390.6870.9170.2760.4550.4550.161400.2180.2220.0160.0160.1550.2550.4040.6160.6370.7870.8370.816

800.1340.1590.6320.8060.1750.2240.5510.9450.1640.3010.3310.097800.0160.0360.030.0250.8670.0650.0250.0220.7430.0670.020.021

1600.1060.1130.4790.8340.110.1410.3970.9140.0970.1780.2120.0571600.020.0540.0460.030.8730.110.0380.0310.7390.1170.0320.03

3200.0630.0630.3020.7790.0650.0810.2390.8810.0620.1020.1340.0353200.0250.0850.0660.0420.8760.1590.0530.2650.7810.1860.050.037

6400.0370.050.1860.7810.2650.0530.1590.8760.0420.0660.0850.0256400.0350.1340.1020.0620.8810.2390.0810.0650.7790.3020.0630.063

12800.030.0320.1170.7390.0310.0380.110.8730.030.0460.0540.0212800.0570.2120.1780.0970.9140.3970.1410.110.8340.4790.1130.106

25600.0210.020.0670.7430.0220.0250.0650.8670.0250.030.0360.01625600.0970.3310.3010.1640.9450.5510.2240.1750.8060.6320.1590.134

IP0.8160.8370.7870.6370.6160.4040.2550.1550.0160.0160.2220.218IP0.1610.4550.4550.2760.9170.6870.3390.2610.8910.7460.260.229

8001600320064001280025600blankpoolnguoc

19-Oct

oral6-16-26-3

400.3370.5430.6540.1310.3350.6220.5050.1330.4390.5240.8141.008

800.2150.3670.4540.0750.2140.4440.3640.0880.2820.3390.6310.832

1600.1210.2240.3350.0510.1330.2890.2520.0510.1960.2130.4590.63

3200.0680.1360.2150.0320.0870.1810.1680.030.1040.130.3110.448

6400.0460.0850.1350.0210.0530.1130.1040.0220.0670.0820.1990.29

12800.030.0550.0850.0130.0360.0710.0670.0180.0480.0550.1270.163

25600.0220.0320.0520.0120.0240.0470.0430.0150.0330.0370.080.091

IP1.1281.0590.990.8310.630.380.2250.130.0150.0130.2080.201

8000160003200064000128000256000blankpool

6-46-56-6

400.2170.2660.7540.9570.2010.3390.4820.7680.2510.3170.5440.912

800.1430.1870.5540.8670.1320.2230.3330.5760.1690.2150.380.669

1600.0920.1180.3750.7430.0870.1460.2210.3640.1080.1460.2530.38

3200.0670.080.2560.6610.0640.1010.1580.2030.0720.1020.1820.189

6400.0470.0540.1620.5510.0440.0650.1070.1130.0540.0780.1270.106

12800.0410.0420.1040.4380.0320.0450.0750.0660.0390.0480.0880.066

25600.0240.0260.0640.3010.0220.0290.0520.0390.0270.0330.0570.071

IP1.0181.0140.9340.7850.6310.4160.2410.1340.0120.0110.1190.101

8000160003200064000128000256000blankpool

6-76-84-8serum

400.2940.6250.9521.1770.2510.4830.5391.1950.070.1970.2670.151

800.1450.3670.7421.10.1310.2840.3411.0920.0440.0820.1260.086

1600.0960.2290.5471.020.0820.1810.2191.0480.0320.0480.070.055

3200.0590.1460.3560.8890.0560.1230.1431.0430.0240.0290.0410.036

6400.0410.0920.2380.6640.040.0880.0950.9490.0210.0220.0260.027

12800.0320.0630.1510.4310.0280.0560.0620.7820.0170.020.0220.026

25600.0220.0410.0930.2490.0220.0350.0420.5610.0160.0180.0180.02

IP1.1361.1071.0320.9280.7370.5020.3120.1710.0150.0150.2010.214

8000160003200064000128000256000blankpool

20-Oct

1-41-51-6

400.2590.2490.3480.0890.2570.2620.3690.0680.240.4570.4070.111

800.1690.1520.2220.0490.1550.1510.2330.0390.1370.2740.2370.066

1600.0930.0830.1330.0360.0830.0850.1460.0310.0740.1550.1470.048

3200.050.0470.0830.0220.0490.0560.0920.0210.0450.0890.0930.027

6400.030.0290.0490.0170.0340.0350.0580.0170.030.0510.0550.019

12800.0250.0230.0350.0140.0260.0240.0390.0150.0260.0370.0390.021

25600.0160.0160.0210.0110.0160.0180.0250.0130.0170.0250.0260.015

IP0.6350.6230.5710.5030.3750.2330.1570.090.0120.0120.0940.087

8000160003200064000128000256000blankpool

3-13-23-3

400.4710.6030.6970.2350.4550.6910.7760.1680.5190.7990.8140.976

800.3830.4560.5810.120.3550.5360.7050.1080.4620.7240.7580.833

1600.2340.2680.4210.0740.2160.3290.4850.0720.3020.5170.5950.612

3200.1240.1460.2470.0410.1120.1880.3150.0480.1690.2910.3910.35

6400.0730.0910.1530.0280.0680.1210.2030.0330.1030.1770.2460.163

12800.050.0620.0790.0260.0510.0750.130.0310.0720.1090.1460.095

25600.0370.0410.0610.0240.0350.050.0830.0270.0510.0680.0850.056

IP0.8820.9020.80.7410.5870.390.2490.1510.0220.0210.2040.216

8000160003200064000128000256000blankpool

3-43-53-6

400.4940.6390.7020.180.5470.6750.8251.0670.510.5910.9050.954

800.3630.4440.5780.1050.4170.4030.7020.9660.3840.3910.7730.717

1600.2250.2580.3480.070.2710.3030.5120.8710.2560.2120.6050.469

3200.1210.1450.2610.0450.1430.1730.3320.7770.1410.1220.3870.256

6400.0690.090.1420.0290.0840.1010.1920.6370.0850.0730.2350.13

12800.0480.0620.0850.0260.0560.0650.1210.4720.0580.0530.1460.087

25600.0330.040.0610.0210.0380.0450.0760.3130.0410.0390.0890.056

IP0.9430.8730.7290.6890.5570.3780.2520.1420.020.0190.1270.308

8000160003200064000128000256000blankpool

3-73-81-3

400.3660.4910.5890.8070.360.5190.6990.8970.3740.440.5970.077

800.2750.3270.4510.7790.2550.3540.5780.870.2480.2970.4410.052

1600.1610.1870.2990.7070.1420.2120.4170.8860.1430.1720.2940.037

3200.0860.1130.1830.5990.0870.1240.2710.8890.0890.1060.1880.028

6400.0490.070.10.4530.0530.0730.1620.8420.0580.0670.1190.024

12800.0330.0490.0690.2950.0390.0530.1070.8090.0410.0460.0830.028

25600.0250.0360.0460.1750.0280.0350.0640.7760.030.0340.0550.022

IP0.7380.7870.6420.6450.5160.3590.2310.1370.0170.0170.1220.118

8000160003200064000128000256000blankpool

22-Oct9-19-29-3

400.4020.6140.8030.9320.4870.5890.7130.9470.5340.6620.7811.02

800.2360.4080.690.8910.2810.4530.550.8950.3560.4510.6320.956

1600.1290.230.5020.8950.1390.2830.3480.8450.2050.2740.4240.896

3200.0690.1260.30.8430.0750.1480.2020.7460.1110.1480.2610.758

6400.0420.0780.1790.7870.050.0870.1230.5750.0690.0870.1520.567

12800.030.0460.1040.7040.0360.0580.0770.3940.0510.0580.0980.349

25600.0210.0340.0610.5660.0270.0350.0470.2330.0340.0380.060.195

IP0.8870.880.8050.7260.5940.420.2680.1520.0180.0170.2210.193

8000160003200064000128000256000blankpool

9-49-59-6

400.4280.4860.6720.9150.4550.4470.70.8230.4970.6030.7231.004

800.2850.3370.5280.8590.2910.2790.5160.5880.3440.4060.5140.986

1600.1480.1890.3540.7740.1540.1530.3520.4150.2030.2320.3070.94

3200.0780.1030.2080.6920.0790.0840.2040.2710.1090.1250.1810.846

6400.0490.0610.1290.5790.050.050.1260.1560.0630.0680.1040.738

12800.0360.0450.0890.4840.0340.0350.0790.0970.0420.0440.0680.603

25600.0230.0270.0510.3150.0240.0220.0480.0610.030.0320.0420.424

IP0.8980.860.7970.7380.6450.4690.3180.1910.0170.0180.210.203

8000160003200064000128000256000blankpool

9-79-810-1

400.7250.7650.8430.8030.5130.5960.8290.9550.5150.6940.7681.041

800.6320.6530.7690.6870.3580.3970.6690.8620.2610.4070.5320.981

1600.3890.3860.5480.5290.1870.1940.3840.6670.1180.1930.310.898

3200.2080.2150.3360.3260.0970.0990.2170.3860.0670.1080.1760.69

6400.1130.1250.1890.1860.0570.0590.1270.2310.0430.0660.1050.448

12800.0710.0730.1120.1020.0380.0420.0770.1380.0330.0420.0660.258

25600.0450.0470.0660.0580.0270.0280.0490.0790.0240.030.0420.168

IP0.9070.8880.8160.7260.5820.3920.2520.1480.0180.0180.1760.218

8000160003200064000128000256000blankpool

10-210-310-4

400.360.5340.5790.8790.3530.4320.5240.8720.4420.3970.581.06

800.2190.3590.4260.830.1990.2590.3690.7440.270.220.4011.024

1600.1340.2250.30.7380.1150.1540.2570.5610.1610.1350.2690.856

3200.0910.1480.2020.5550.0730.1020.1740.3580.0980.0870.1830.91

6400.0640.0990.1360.3360.0520.070.1210.2030.0720.0650.1240.782

12800.0460.0630.0880.1850.040.050.080.120.050.0440.0840.631

25600.0320.0450.0610.1070.0290.0370.0570.0720.0390.0330.0560.415

IP0.9550.9460.860.7340.60.3930.2360.1350.0190.0190.210.247

8000160003200064000128000256000blankpool

10-510-610-7

400.4690.3270.5451.020.350.4030.5661.0090.3890.4750.5940.948

800.3320.2170.4320.9950.2040.2610.4190.9550.240.3250.4380.922

1600.1830.1270.3020.9390.1140.1550.2820.8710.1420.20.2940.763

3200.1170.0830.1860.8740.0710.0970.1810.7390.0880.1180.190.683

6400.0720.0530.1190.780.0480.0610.120.5110.0580.0760.1170.356

12800.0520.0390.0850.6290.0380.0460.0830.320.0440.0540.0820.252

25600.0350.0290.0520.4410.0280.0290.0510.1740.0390.0420.0550.137

IP0.9320.7490.8380.7270.5930.3960.2940.1840.0160.0160.2440.207

8000160003200064000128000256000blankpool

23-Oct10-811-111-2

400.4890.5680.7010.9330.380.4080.6340.9720.4270.5170.771.061

800.4860.3980.6190.8810.2380.2760.5170.8940.2850.3680.6451.027

1600.3280.2190.460.8320.1160.1550.3360.8510.1550.2320.4921.024

3200.2150.1320.330.7410.0690.10.2150.7550.0950.1390.30.968

6400.1250.0760.1990.6070.0450.0640.1260.6180.0620.0860.1750.955

12800.0810.0510.1210.4450.0310.0430.0780.4590.0440.0580.1080.873

25600.0490.030.0720.2720.0210.0280.0450.2860.0310.0380.0660.737

IP0.8630.8240.7570.6850.5230.3640.2140.1170.0190.0210.1510.252

8000160003200064000128000256000blankpool

11-311-411-5

400.3530.4680.6740.9590.4590.5660.770.970.5810.5450.6691.081

800.2540.3280.560.9250.2960.4140.6490.9730.370.4430.5760.98

1600.1420.1810.3780.9130.1640.2440.4810.9470.2330.250.4190.926

3200.0880.1010.2130.8520.0860.1420.2830.9060.1290.1340.2440.81

6400.0530.0580.1160.8220.0510.0820.1570.8520.0710.0750.140.642

12800.0360.0410.0720.6960.0370.0540.0950.7910.0490.0490.0850.414

25600.0270.0290.0460.5660.0270.0350.0610.6840.0320.0330.0520.212

IP0.8550.8450.7870.7090.5830.3760.2340.1260.0190.0190.2550.165

8000160003200064000128000256000blankpool

11-611-711-8

400.2050.3210.5250.8690.2890.3470.4970.9010.3020.3830.7450.982

800.1460.2380.3960.8120.1890.2560.3870.8450.1870.2860.6160.96

1600.0840.1570.2850.7920.1250.1780.2880.840.1190.2080.4480.937

3200.0720.1180.190.7740.0830.1170.1890.8460.0750.1360.3070.976

6400.040.0560.1120.6790.0530.0680.1070.690.0530.080.1710.905

12800.0340.040.0580.5340.0320.0380.0590.4390.0350.0430.0830.659

25600.0240.0250.0470.5530.0280.0340.0480.40.030.0390.0620.772

IP0.8880.8270.830.7250.6220.3790.260.1620.0190.0190.2150.131

8000160003200064000128000256000blankpool

12-112-212-3

400.2460.2140.430.9720.370.420.5510.9170.3590.410.5460.928

800.1340.1440.3020.8980.2570.2920.4830.8430.2710.3060.4480.883

1600.0820.1190.2130.8590.1670.20.3640.7690.1880.2190.3480.823

3200.0580.0790.150.8010.1030.1330.2480.6370.1250.1450.2280.712

6400.0410.0520.0990.6510.0720.0860.1590.4490.0860.0890.1510.609

12800.0370.0520.0670.4640.0530.060.10.2890.0620.0590.0990.426

25600.0280.0290.0460.2780.0360.040.0640.1630.0420.0430.0620.256

IP0.9240.8960.870.7850.6950.4850.3240.1940.0220.0210.2670.099

8000160003200064000128000256000blankpool

12-412-512-6

400.4010.3880.7340.9940.3170.370.8240.9680.4930.5320.6520.853

800.2630.2670.6470.9930.2150.2680.7610.9660.3960.4010.4810.785

1600.1450.150.5150.9760.140.1670.6670.9730.2670.2910.4180.627

3200.0890.1280.3550.9340.090.1110.5240.9690.1680.1920.3030.461

6400.0630.0630.2170.8820.0580.0690.3540.9230.1020.0980.1980.239

12800.0510.0460.140.8140.0440.050.2150.9080.0740.0880.1340.126

25600.0370.0380.090.7590.0340.0370.1310.8720.0530.0550.0850.078

IP0.9440.8120.8880.7870.6290.4320.2980.1760.0160.0190.1280.132

8000160003200064000128000256000blankpool

23-Oct12-712-816-1

400.1790.3210.5970.9060.3560.3360.5220.8330.3330.3020.7530.39

800.1920.2820.5250.90.2430.2660.4650.7460.2440.270.6720.275

1600.1140.1370.3980.80.1530.1910.2770.6420.1660.2170.5360.173

3200.0850.1040.2960.7810.1050.1320.2370.4960.1160.1630.4240.098

6400.0550.0670.1790.6770.0650.0810.1540.2930.0760.1020.2590.073

12800.0370.0420.1120.6040.0440.0550.1010.1790.0560.0640.1760.04

25600.0270.0320.070.5420.0330.0410.0730.1050.0410.0490.1130.034

IP0.8340.7680.8170.6340.620.4440.2850.1780.0210.020.2010.103

8000160003200064000128000256000blankpool

16-416-716-8

400.2770.3390.6280.7770.3640.3680.6220.7440.4090.3360.5880.368

800.2440.2230.5860.640.2390.2740.4820.6330.2920.2420.4880.245

1600.170.1540.4890.5630.1510.1930.3950.4330.1940.1970.3620.147

3200.1050.1390.3560.5660.1060.1310.30.270.1240.1150.2420.089

6400.0770.0990.2250.4660.0620.0840.160.1570.080.070.1670.066

12800.0460.0390.1380.230.0460.0560.1210.0830.0550.0580.1090.036

25600.0320.0340.0810.1410.030.0370.0630.0470.0390.0310.0680.023

IP0.880.8220.8140.7140.5960.3950.2250.1580.0230.0190.1450.193

8000160003200064000128000256000blankpool

17-117-617-7

400.2790.2870.6970.8830.2470.2690.7520.9610.3380.4760.7120.99

800.2380.2230.630.7250.1630.2090.5920.8990.2410.3250.6160.923

1600.1420.1750.5350.7180.1290.1740.5180.9120.1820.280.530.874

3200.0990.1270.3890.680.0890.1260.470.9150.1250.1940.4030.902

6400.0640.0860.2880.5770.0590.0850.3020.8220.0780.1460.2790.833

12800.0440.080.1950.5060.0470.0630.1680.780.0610.0820.1670.707

25600.030.0570.1190.3370.0340.0460.1280.6340.0390.0590.1090.66

IP0.8570.8290.8150.7430.5690.4180.2080.1950.0250.0210.2150.19

8000160003200064000128000256000blankpool

17-818-118-2

400.190.2240.7240.8450.2710.3770.6080.7820.2390.3710.6780.318

800.1580.1810.6450.7890.190.2890.5570.7430.1850.2660.5790.226

1600.1110.1610.5730.7240.1470.2240.3990.660.130.2240.480.122

3200.080.1240.4670.6830.0950.1540.3110.5550.0880.1520.3280.076

6400.0490.0760.3210.5640.0690.1170.2390.3950.060.0930.2150.045

12800.0380.0440.2020.380.0490.0780.1720.2390.0480.0750.1450.033

25600.0280.0310.1060.2050.0310.0460.0830.1450.0330.0450.0960.019

IP0.8010.7650.750.6430.5820.3740.2160.1640.0180.0150.1720.163

8000160003200064000128000256000blankpool

18-318-418-5

400.1820.2380.5790.6740.1830.3220.6190.8560.330.2890.5930.822

800.170.2760.5260.6640.130.2440.5360.890.2490.2010.5460.776

1600.1110.1610.4540.550.0930.1690.4780.8580.1760.1320.4080.683

3200.0770.1190.3460.4060.0710.1210.2970.8160.1170.0870.290.503

6400.050.0830.2360.2910.0420.0860.210.7450.080.0770.220.432

12800.0350.0550.1520.1550.0290.0530.1130.670.0560.050.1390.255

25600.0220.0240.090.0690.0220.0350.090.5750.0360.0460.0890.122

IP0.7860.7710.7340.6640.5490.3620.2340.1370.0210.0120.1410.097

8000160003200064000128000256000blankpool

24-Oct18-718-82-1

400.0640.1250.3150.7260.0640.1270.3150.230.0830.1440.1360.088

800.050.0960.2340.5370.0520.1040.2270.1550.0670.1090.0940.055

1600.0330.0650.160.3280.0390.0780.1840.1030.0490.0860.0720.037

3200.0240.0530.1170.1870.0310.0590.1310.0550.0350.0610.0440.024

6400.020.0430.0840.1110.0230.0460.0940.0390.0280.0450.030.017

12800.0170.030.060.0570.0180.0310.0640.0290.0250.0350.0260.017

25600.0160.0230.0360.0310.0140.0230.0380.0170.0170.0240.0180.015

IP1.0611.0380.9530.8020.6840.4250.2560.1380.0130.0140.0580.084

8000160003200064000128000256000blankpool

7-17-27-3

400.0780.0950.1120.0490.050.0430.0840.0710.0480.070.1240.108

800.0530.0610.0820.0350.0380.0360.0640.0410.0340.0450.0890.065

1600.0370.0390.0550.0220.0260.0250.0430.0260.0220.0310.0630.048

3200.0280.0260.0370.0130.020.0180.0330.0170.0150.0210.0390.03

7-47-57-6

400.0710.0730.1120.0540.060.0720.1260.0730.0740.0660.1360.079

800.0570.0530.080.0290.050.0510.0890.0410.0580.0410.1030.052

1600.050.0370.0630.0210.0320.0370.0590.0340.0430.0410.0680.041

1.0070.9640.8550.6670.4660.240.1570.0870.0110.0140.0650.085

8000160003200064000128000256000blankpool

13-113-213-3

400.0640.110.3230.120.0750.1260.2790.1510.0580.1170.3370.246

800.0470.080.2460.080.0550.0960.2040.1110.0460.0930.2680.171

1600.030.0540.1690.0390.040.0660.150.0620.0340.0720.2020.109

3200.0240.0390.1260.0280.0290.0510.1120.0430.0250.0470.1310.054

13-413-513-6

400.0580.0990.2690.1160.0610.0890.2880.1390.0760.1460.3850.222

800.0420.0750.1980.080.0420.0670.2050.080.0530.1020.2670.148

1600.0320.0560.1390.0460.0320.0510.160.0490.0410.0790.2030.119

0.9850.9270.9190.8330.7220.4830.3390.1890.0090.0070.0570.071

8000160003200064000128000256000blankpool

2-22-32-4

400.0730.120.1320.0670.1030.110.1370.1150.0880.130.1650.127

800.0560.0780.0970.0420.0740.0830.1010.0730.0650.0890.1140.079

1600.0390.0540.0660.0290.050.0580.0720.050.0430.0630.080.048

3200.030.0430.0480.0210.0370.0430.0520.0370.0330.0440.0570.034

2-52-62-7

400.0720.1140.1660.1170.0570.0920.1060.1060.0790.1250.1310.123

800.0650.0870.1260.0750.0430.0660.0790.0620.0620.0920.0970.082

1600.0480.0640.0910.0470.0330.050.0570.0390.0440.0650.0660.056

0.7710.7980.8090.7680.6380.3630.2510.150.0130.0130.0670.078

8000160003200064000128000256000blankpool

8-18-28-3

400.060.0820.1360.0890.0550.0640.1460.8420.0620.0880.1260.067

800.0490.0610.1030.0510.0440.0460.1050.6720.0450.060.0880.047

1600.0280.0340.0620.0310.0290.0320.0670.460.0310.0430.0610.032

3200.0260.0290.0510.020.0250.0270.0580.2840.0250.0320.050.023

8-48-58-6

400.0820.0980.1270.6770.0590.0570.0970.1130.0870.1090.1590.199

800.0680.0660.0990.4510.0470.0430.0720.0630.0670.0780.1160.123

1600.0450.0320.070.2490.0330.0320.0490.0350.0480.0550.080.059

0.8880.8330.860.7650.5730.340.210.1110.0070.0070.090.107

8000160003200064000128000256000blankpool

14-114-214-3

400.0890.1080.1780.0840.0770.0660.1330.2770.0730.0840.1310.116

800.0520.0570.1370.0480.0520.0470.0880.1210.0480.0520.0890.072

1600.0360.0520.0940.0310.0370.0320.0620.0450.0310.0360.0610.041

3200.0260.030.070.0210.0280.0270.050.0340.0230.0280.0440.025

14-414-514-6

400.0850.0680.1490.0790.0550.060.1360.0840.0650.0740.1760.114

800.0630.0530.1080.0470.0370.0410.0870.0430.0440.050.1220.067

1600.040.030.0750.0280.0270.0250.060.0230.0320.0370.0830.036

0.8840.6680.7730.6510.4360.2330.1390.0620.0070.0050.0960.096

8000160003200064000128000256000blankpool

25-Oct10-110-210-3

400.1330.2210.3150.9290.1710.2480.3710.9110.1570.2540.3640.927

800.0980.1510.2130.9140.1210.1690.270.8710.1140.1760.2640.872

1600.0660.1080.1550.9280.0880.1160.1780.790.0750.1280.1850.73

3200.0470.0790.1120.8480.070.0750.120.6140.0580.0830.1320.464

6400.0370.0550.0720.6780.0470.0510.0830.3890.0450.0640.0880.3655

12800.0270.0410.0510.4270.0420.0350.0550.20.0360.0440.0690.141

25600.0220.0270.0340.2340.0290.0250.0370.1070.0280.0310.0410.078

IP1.0280.9540.8950.7930.6230.360.2340.1220.0150.0160.180.176

8000160003200064000128000256000blankpool

10-410-510-6

400.1730.1690.3240.9890.2020.1410.3531.0790.1540.2020.3661.114

800.1180.1110.21610.1350.0920.2341.0070.1080.1360.2521.015

1600.0840.0840.1551.0090.0990.0660.1610.9730.0730.0950.1730.913

3200.0550.0550.1060.8710.0650.0420.1070.8460.0520.0650.110.699

6400.0440.0420.0720.7750.0470.0290.0670.7690.0380.0420.0710.449

12800.0340.0330.0490.6360.0320.0250.0470.5980.0340.0310.0520.26

25600.0320.0270.0360.4220.0260.020.0290.3770.0220.0240.0330.136

IP1.1411.0751.0120.840.7930.5370.3360.1940.0160.0140.1870.191

8000160003200064000128000256000blankpool

10-710-811-1

400.1960.2330.3320.9860.2930.2260.5171.1090.1410.160.3711.154

800.1320.1560.2080.9090.2020.1520.3751.0470.1030.1140.2641.136

1600.0840.0940.1550.8640.1480.1090.2670.9120.0720.0830.1761.002

3200.0620.0610.1030.6750.0980.0740.1760.8810.0530.060.1191.015

6400.0460.0410.070.4240.0650.050.1180.6820.0410.0430.0780.835

12800.0350.0320.0490.2490.0480.0380.0790.4390.0380.0350.0570.571

25600.0260.0260.0340.1340.0360.0290.0550.2590.0310.0290.0440.345

IP1.0930.9171.0250.9010.7160.5220.3170.1950.020.020.1940.198

8000160003200064000128000256000blankpool

11-211-311-4

400.1330.1180.8681.0420.1220.1890.4171.0510.1640.2310.9421.197

800.0940.1080.5791.0420.0850.1370.2850.9950.1090.1540.6531.066

1600.0630.0640.3931.0040.0640.10.20.9850.0730.1090.3811.026

3200.0440.0350.2261.0120.0480.0690.1320.9920.0510.0740.2251.016

6400.0330.030.1370.9650.0330.0480.0870.9460.0430.050.1340.984

12800.0260.0220.0840.9080.0250.0370.0610.860.0320.0410.0790.847

25600.0230.0220.0520.7030.0220.0270.0410.6460.0230.0280.0510.644

IP0.9230.8280.9650.8390.6650.4470.2830.160.0190.0180.1940.208

8000160003200064000128000256000blankpool

11-511-611-7

400.1730.1230.2920.9370.1250.1810.5191.0640.1410.1840.3421.083

800.1080.0610.1930.9260.0850.130.30.8160.090.1210.2090.89

1600.0740.0440.1390.8630.0640.0980.2130.9060.0680.0890.1420.878

3200.0540.0360.0950.7980.0460.0690.1360.9150.0490.0660.0970.905

6400.0450.0350.0730.650.0380.0520.0980.9130.040.0530.070.797

12800.0350.0280.050.4060.030.0380.0620.7620.0350.040.0480.527

25600.0250.0150.0350.2120.0220.0280.0420.5520.0260.0330.0340.289

IP0.9050.7350.9020.7610.6630.490.2960.1780.0250.0250.2020.209

8000160003200064000128000256000blankpool

11-812-112-2

400.1630.1360.8810.9970.1060.1690.3950.9970.2190.2940.4981.089

800.1120.1040.740.9520.0690.1310.2420.9840.1480.2160.3860.917

1600.0760.0770.4850.9330.0570.0960.1490.9170.0990.1490.2740.8

3200.0550.0650.2830.8850.0440.0650.1040.8370.0690.0950.1770.53

6400.040.0450.1680.8430.0340.0490.0720.6530.0510.0630.1140.312

12800.0340.0320.1020.8470.0310.0410.0520.4120.0450.0510.0820.175

25600.0320.0190.0660.7330.0320.0330.0420.2440.040.040.0580.099

IP1.0631.0180.970.9050.7020.470.2820.1630.0310.0320.2450.24

8000160003200064000128000256000blankpool

12-312-412-5

400.2010.1580.4180.9750.1780.230.820.9240.1530.2140.8950.947

800.1270.1010.2980.8930.1210.1590.6540.990.0980.1350.7730.975

1600.0830.0890.2070.9130.0760.1080.4330.9390.0690.0930.5670.943

3200.0580.0530.1450.7930.0550.070.260.9460.0460.0580.3250.989

6400.0420.0340.0930.7280.0410.0510.1660.8820.0350.0410.2040.943

12800.0370.0260.0650.5260.0370.0370.0930.9120.0250.0340.1190.953

25600.0250.0210.040.2970.0210.0270.0550.7440.0220.0240.0670.854

IP0.9570.8990.8960.780.6660.4480.2620.1580.0940.0210.2080.209

8000160003200064000128000256000blankpool

12-612-712-8

400.2240.1730.4470.8280.1230.2120.460.9160.180.2180.4750.885

800.130.1210.3470.7750.0750.130.350.9670.120.1570.3870.801

1600.0620.0860.2440.5960.0580.1020.2150.9380.0670.1120.2960.647

3200.0480.0730.1640.3590.0490.0690.1390.8450.0540.0770.2020.417

6400.0330.0450.1080.1850.0340.0480.0910.7870.0360.0550.1310.235

12800.0290.0360.070.1030.0260.0350.0550.6430.0350.0370.0790.121

25600.0220.0190.0460.0590.0180.0280.0390.5160.0210.0290.0520.07

IP0.8610.7540.8660.8030.6860.5320.30.2050.0140.0180.1710.174

8000160003200064000128000256000blankpool

26-Oct9-19-29-3

400.2090.3580.8090.9610.2570.3650.4670.9780.2910.3220.5741.068

800.110.1920.620.940.1440.2330.2960.8870.1710.1930.3910.994

1600.0680.110.4260.9080.0870.1460.1880.7820.1050.1120.2460.923

3200.0460.0730.260.9030.0680.0870.1190.5710.0660.0740.1520.789

6400.0320.0460.1570.8130.0410.0570.0810.3280.0470.0460.0930.53

12800.0250.0330.0860.610.0290.0370.0490.1720.0330.030.0590.315

25600.0180.0210.050.3780.0180.0240.0310.0890.0230.0230.0390.17

IP0.9950.9690.8730.7770.6240.4060.2460.130.0120.0120.2220.205

8000160003200064000128000256000blankpool

9-49-59-6

400.1840.1880.6090.9770.1790.1510.3140.9390.2140.2710.4391.184

800.1010.1180.4110.8820.1110.090.2080.7580.1350.1650.2971.079

1600.0660.0740.2890.820.0790.0560.1360.540.0850.0930.1910.967

3200.0420.0430.1640.7840.0460.040.0880.3510.0520.0610.1120.933

6400.0290.0320.1050.6930.030.0280.0540.1910.0350.0350.0730.863

12800.0210.0230.0610.6060.0250.0230.0340.1090.0260.0250.0480.739

25600.0170.0140.0320.4250.0170.0150.0240.0630.0190.020.0320.523

IP0.9070.8840.850.7390.6110.4240.2470.1430.0110.0090.1830.194

8000160003200064000128000256000blankpool

9-79-816-1

400.3760.3580.5240.9020.1980.1850.360.9260.1920.2370.6070.27

800.2610.2350.3580.7410.1310.1180.2280.8140.1250.1680.4350.135

1600.1710.1540.2450.5920.0870.080.1530.5850.0880.1210.3140.077

3200.110.1040.1690.3770.0650.0580.1050.3250.0630.0870.2010.047

6400.0750.0650.1040.1960.0370.0390.0690.1690.0430.0540.1410.026

12800.0520.0460.0690.1120.0320.0290.0410.1050.0340.040.0880.023

25600.030.030.0480.060.020.0190.0280.0570.0230.0280.0590.017

IP0.9550.9230.920.8270.6550.4480.2630.1620.0140.0120.2030.179

8000160003200064000128000256000blankpool

5-15-25-3

400.2120.1620.8951.0410.2270.3070.4360.8770.2290.2681.0111.074

800.1110.0920.7351.0280.1390.1910.3090.6560.1240.150.8351.007

1600.060.060.5931.0480.090.1250.2110.4290.0760.0910.6981.08

3200.0350.0390.3641.0010.0590.0830.1380.2370.050.0580.4521.086

6400.0280.0290.2130.9620.0390.0530.090.1250.0330.0370.2591.045

12800.0210.0210.1250.8870.0280.0380.060.070.0260.0290.1441

25600.0120.0150.0690.8570.020.0240.0370.0390.0180.020.0781.004

IP0.9290.9260.9070.8170.6730.4450.2850.1560.010.010.1850.143

8000160003200064000128000256000blankpool

5-45-55-6

400.0990.1490.2640.1420.1360.1860.3490.7160.1760.2210.3450.788

800.050.0930.1660.070.0860.1160.2330.5490.1070.1360.2250.679

1600.040.0670.1120.0460.0610.0740.1610.4160.0670.0880.1520.586

3200.0250.0460.0720.0260.0420.0570.110.3260.0440.0570.0980.419

6400.0210.0280.050.020.0620.040.0790.1430.0330.040.0680.298

12800.0180.0230.0340.0190.0240.030.0510.0960.0290.030.0460.179

25600.0110.0170.0230.0120.0170.0210.0330.0650.020.020.0280.096

IP0.8370.9120.8850.7550.6350.4030.2340.120.0130.0140.2230.135

8000160003200064000128000256000blankpool

5-75-86-4

400.0990.1780.7791.0290.130.1940.6560.9690.1610.2170.6390.677

800.0490.1030.5460.9960.0780.1240.4481.0390.1030.1350.4680.618

1600.0380.0690.3051.0060.0550.0820.3040.9050.070.0930.3110.534

3200.030.0460.1651.0190.0420.0580.1920.8940.0510.0630.2140.365

6400.0210.0340.1150.9890.0270.0370.1240.8610.0370.0410.120.264

12800.0210.0270.0690.9570.0210.0280.0780.8220.0290.030.0830.223

25600.0160.0190.0440.8820.0150.0180.0430.7780.0180.0210.0480.155

IP0.8270.8690.8480.7910.6540.3860.2660.110.0130.0140.1660.114

8000160003200064000128000256000blankpool

6-16-26-3

400.10.2040.3390.1380.1210.2390.2250.0510.1540.2290.3530.569

800.0660.1240.2150.0670.0750.1360.1460.0240.0950.160.2230.375

1600.0280.0720.1470.0460.0540.0890.10.0170.0690.0950.220.371

3200.030.0560.1050.0280.0410.0660.0710.0120.0480.0660.0930.159

6400.0190.0350.0720.0210.030.0470.0450.0110.0340.0410.0970.106

12800.0180.0270.050.0190.0220.0330.0330.010.0290.0310.0610.054

25600.0120.020.0340.0140.0170.0240.0220.0090.0190.0230.0380.031

IP0.8730.910.8890.8080.6820.4250.2850.1370.0110.0130.2260.113

8000160003200064000128000256000blankpool

GST-IgG

5-45-55-618-Oct4-14-24-3

400.2680.3010.4560.2070.2560.3540.5230.9410.3430.3870.4950.942400.3920.3640.4730.6950.2820.3210.5170.2370.380.3430.5320.302

800.2080.1930.3320.1120.170.2240.3760.8520.2260.2470.3750.849800.2910.240.370.6020.2010.2270.2750.190.2790.240.420.19

1600.1030.1130.2160.0550.0970.1220.2490.8350.1380.1430.2450.7671600.1730.1540.2550.4170.1270.1630.2560.1290.1830.150.2950.106

3200.0780.0650.1360.0280.0620.0770.1630.7280.0780.0870.1550.6693200.0950.0920.1540.2460.0770.1030.1620.0850.1060.0960.1810.063

6400.050.0450.0820.0220.0410.0510.0990.6370.050.0510.0910.5316